Restriction map for
pF1KB7024
(Circular) (Six-base) MAPSORT of: pF1KB7024.seq Check: 6002 from: 1 to: 4391
pF1KB7024 4391 bp
With 182 enzymes: SgfI *
February 20, 2009 16:23 ..
AccI GT'mk_AC
Cuts at: 1429 1429
Size: 4391
Acc65I G'GTAC_C
Cuts at: 1407 1407
Size: 4391
AclI AA'CG_TT
Cuts at: 4231 4231
Size: 4391
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 436 2216 2648 3001 3663 403
Size: 33 1780 432 353 662 1131
Fragments arranged by size:
1780 1131 662 432 353 33
AflIII A'CryG_T
Cuts at: 1743 3543 3824 1743
Size: 1800 281 2310
Fragments arranged by size:
2310 1800 281
AleI CACnn'nnGTG
Cuts at: 888 888
Size: 4391
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 319 319
Size: 4391
AlwNI CAG_nnn'CTG
Cuts at: 790 1349 1696 3134 790
Size: 559 347 1438 2047
Fragments arranged by size:
2047 1438 559 347
ApaI G_GGCC'C
Cuts at: 520 520
Size: 4391
ApaLI G'TGCA_C
Cuts at: 3229 3229
Size: 4391
ApoI r'AATT_y
Cuts at: 1395 3744 4000 1395
Size: 2349 256 1786
Fragments arranged by size:
2349 1786 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4391
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
AvaI C'yCGr_G
Cuts at: 270 519 670 1411 270
Size: 249 151 741 3250
Fragments arranged by size:
3250 741 249 151
BamHI G'GATC_C
Cuts at: 1416 1416
Size: 4391
BanI G'GyrC_C
Cuts at: 310 349 367 525 1407 1821 2069 2104 310
Size: 39 18 158 882 414 248 35 2597
Fragments arranged by size:
2597 882 414 248 158 39 35 18
BanII G_rGCy'C
Cuts at: 520 953 1405 2435 520
Size: 433 452 1030 2476
Fragments arranged by size:
2476 1030 452 433
BbeI G_GCGC'C
Cuts at: 353 371 529 1825 2073 353
Size: 18 158 1296 248 2671
Fragments arranged by size:
2671 1296 248 158 18
BbsI GAAGACnn'nnnn_
Cuts at: 922 922
Size: 4391
BciVI GTATCCnnnnn_n'
Cuts at: 2289 3345 4391 2289
Size: 1056 1046 2289
Fragments arranged by size:
2289 1056 1046
BclI T'GATC_A
Cuts at: 1911 1911
Size: 4391
BglI GCCn_nnn'nGGC
Cuts at: 358 358
Size: 4391
BglII A'GATC_T
Cuts at: 1906 3943 1906
Size: 2037 2354
BlpI GC'TnA_GC
Cuts at: 1503 1503
Size: 4391
Bme1580I G_kGCm'C
Cuts at: 520 2016 2109 3233 520
Size: 1496 93 1124 1678
Fragments arranged by size:
1678 1496 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 915 1603 1684 2019 4163 915
Size: 688 81 335 2144 1143
Fragments arranged by size:
2144 1143 688 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2758 2758
Size: 4391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 427 451 726 886 1146 168
Size: 259 24 275 160 260 3413
Fragments arranged by size:
3413 275 260 259 160 24
Bpu10I CC'TnA_GC
Cuts at: 700 700
Size: 4391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 1567 2446 3160 3458 391
Size: 1176 879 714 298 1324
Fragments arranged by size:
1324 1176 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4095 4095
Size: 4391
BsaAI yAC'GTr
Cuts at: 1744 1744
Size: 4391
BsaBI GATnn'nnATC
Cuts at: 6 1905 3942 6
Size: 1899 2037 455
Fragments arranged by size:
2037 1899 455
BsaHI Gr'CG_yC
Cuts at: 141 350 368 526 1822 2070 2772 4268 141
Size: 209 18 158 1296 248 702 1496 264
Fragments arranged by size:
1496 1296 702 264 248 209 158 18
BsaWI w'CCGG_w
Cuts at: 1580 2101 2779 3190 3337 3611 1580
Size: 521 678 411 147 274 2360
Fragments arranged by size:
2360 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 141 171 1144 1174 141
Size: 30 973 30 3358
Fragments arranged by size:
3358 973 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 165 193 196 218 221 925 165
Size: 28 3 22 3 704 3631
Fragments arranged by size:
3631 704 28 22 3 3
BseYI C'CCAG_C
Cuts at: 513 1313 1816 2574 3239 3647 513
Size: 800 503 758 665 408 1257
Fragments arranged by size:
1257 800 758 665 503 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1057 1312 1057
Size: 255 4136
BsiEI CG_ry'CG
Cuts at: 104 362 498 1933 1979 3209 104
Size: 258 136 1435 46 1230 1286
Fragments arranged by size:
1435 1286 1230 258 136 46
BsiHKAI G_wGCw'C
Cuts at: 407 1405 2183 2373 3233 407
Size: 998 778 190 860 1565
Fragments arranged by size:
1565 998 860 778 190
BsmI GAATG_Cn'
Cuts at: 615 615
Size: 4391
BsmBI CGTCTCn'nnnn_
Cuts at: 148 148
Size: 4391
Bsp1286I G_dGCh'C
Cuts at: 407 520 953 1405 2016 2109 2183 2373 2435
Size: 113 433 452 611 93 74 190 62
Cuts at: 2435 3233 407
Size: 798 1565
Fragments arranged by size:
1565 798 611 452 433 190 113 93
74 62
BspHI T'CATG_A
Cuts at: 2823 2823
Size: 4391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1441 1655 1957 2338 1441
Size: 214 302 381 3494
Fragments arranged by size:
3494 381 302 214
BsrBI CCG'CTC
Cuts at: 50 423 2683 2737 4221 4386 50
Size: 373 2260 54 1484 165 55
Fragments arranged by size:
2260 1484 373 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2303 2303
Size: 4391
BsrFI r'CCGG_y
Cuts at: 256 352 532 2389 2570 256
Size: 96 180 1857 181 2077
Fragments arranged by size:
2077 1857 181 180 96
BsrGI T'GTAC_A
Cuts at: 1103 1103
Size: 4391
BssHII G'CGCG_C
Cuts at: 536 536
Size: 4391
BssSI C'ACGA_G
Cuts at: 1255 2662 3370 1255
Size: 1407 708 2276
Fragments arranged by size:
2276 1407 708
BstAPI GCAn_nnn'nTGC
Cuts at: 402 479 1044 1768 402
Size: 77 565 724 3025
Fragments arranged by size:
3025 724 565 77
BstBI TT'CG_AA
Cuts at: 2752 2752
Size: 4391
BstEII G'GTnAC_C
Cuts at: 319 319
Size: 4391
BstYI r'GATC_y
Cuts at: 1 378 606 1416 1883 1906 2241 2487 2793
Size: 377 228 810 467 23 335 246 306
Cuts at: 2793 2805 2891 3943 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 810 467 449 377 335 306 246
228 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 980 980
Size: 4391
BtgI C'CryG_G
Cuts at: 344 449 459 1273 344
Size: 105 10 814 3462
Fragments arranged by size:
3462 814 105 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2341 2522 2685 2341
Size: 181 163 4047
Fragments arranged by size:
4047 181 163
BtsI GCAGTG_nn'
Cuts at: 1634 1634
Size: 4391
DraI TTT'AAA
Cuts at: 1390 1390
Size: 4391
DraIII CAC_nnn'GTG
Cuts at: 402 479 1185 402
Size: 77 706 3608
Fragments arranged by size:
3608 706 77
DrdI GACnn_nn'nnGTC
Cuts at: 2097 3441 2097
Size: 1344 3047
EaeI y'GGCC_r
Cuts at: 359 495 601 1976 2150 2541 2568 3913 359
Size: 136 106 1375 174 391 27 1345 837
Fragments arranged by size:
1375 1345 837 391 174 136 106 27
EagI C'GGCC_G
Cuts at: 359 495 1976 359
Size: 136 1481 2774
Fragments arranged by size:
2774 1481 136
EarI CTCTTCn'nnn_
Cuts at: 2414 2624 2414
Size: 210 4181
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 391 1128 2599 3341 3487 4201 391
Size: 737 1471 742 146 714 581
Fragments arranged by size:
1471 742 737 714 581 146
EcoICRI GAG'CTC
Cuts at: 1403 1403
Size: 4391
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 403 427 436 451 726 886 1146 2216
Size: 235 24 9 15 275 160 260 1070
Cuts at: 2216 2648 3001 3663 168
Size: 432 353 662 896
Fragments arranged by size:
1070 896 662 432 353 275 260 235
160 24 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 295 295
Size: 4391
EcoO109I rG'GnC_Cy
Cuts at: 1530 1530
Size: 4391
EcoRI G'AATT_C
Cuts at: 1395 1395
Size: 4391
EcoRV GAT'ATC
Cuts at: 963 963
Size: 4391
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 4391
FspI TGC'GCA
Cuts at: 548 2172 548
Size: 1624 2767
HaeII r_GCGC'y
Cuts at: 353 371 391 529 1825 2073 3303 4074 353
Size: 18 20 138 1296 248 1230 771 670
Fragments arranged by size:
1296 1230 771 670 248 138 20 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 116 148 2233 2263 2265 2295 116
Size: 32 2085 30 2 30 2212
Fragments arranged by size:
2212 2085 32 30 30 2
HincII GTy'rAC
Cuts at: 1430 1430
Size: 4391
KasI G'GCGC_C
Cuts at: 349 367 525 1821 2069 349
Size: 18 158 1296 248 2671
Fragments arranged by size:
2671 1296 248 158 18
KpnI G_GTAC'C
Cuts at: 1411 1411
Size: 4391
MluI A'CGCG_T
Cuts at: 3824 3824
Size: 4391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3150 3334 3150
Size: 184 4207
MscI TGG'CCA
Cuts at: 2152 2152
Size: 4391
MslI CAynn'nnrTG
Cuts at: 888 2380 2507 888
Size: 1492 127 2772
Fragments arranged by size:
2772 1492 127
MspA1I CmG'CkG
Cuts at: 346 429 461 805 1032 1074 1242 1349 1502
Size: 83 32 344 227 42 168 107 153
Cuts at: 1502 1816 2176 2960 3205 346
Size: 314 360 784 245 1532
Fragments arranged by size:
1532 784 360 344 314 245 227 168
153 107 83 42 32
NaeI GCC'GGC
Cuts at: 354 534 2572 354
Size: 180 2038 2173
Fragments arranged by size:
2173 2038 180
NarI GG'CG_CC
Cuts at: 350 368 526 1822 2070 350
Size: 18 158 1296 248 2671
Fragments arranged by size:
2671 1296 248 158 18
NgoMIV G'CCGG_C
Cuts at: 352 532 2570 352
Size: 180 2038 2173
Fragments arranged by size:
2173 2038 180
NspI r_CATG'y
Cuts at: 1444 1944 3547 1444
Size: 500 1603 2288
Fragments arranged by size:
2288 1603 500
PasI CC'CwG_GG
Cuts at: 835 835
Size: 4391
PciI A'CATG_T
Cuts at: 3543 3543
Size: 4391
PflMI CCAn_nnn'nTGG
Cuts at: 450 450
Size: 4391
PfoI T'CCnGG_A
Cuts at: 944 1162 944
Size: 218 4173
PmeI GTTT'AAAC
Cuts at: 1390 1390
Size: 4391
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2837 2869 2837
Size: 32 4359
PspOMI G'GGCC_C
Cuts at: 516 516
Size: 4391
PstI C_TGCA'G
Cuts at: 511 777 1241 1438 2123 511
Size: 266 464 197 685 2779
Fragments arranged by size:
2779 685 464 266 197
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4391
PvuII CAG'CTG
Cuts at: 429 1032 1074 1349 1816 2176 429
Size: 603 42 275 467 360 2644
Fragments arranged by size:
2644 603 467 360 275 42
RsrII CG'GwC_CG
Cuts at: 2586 2586
Size: 4391
SacI G_AGCT'C
Cuts at: 1405 1405
Size: 4391
SacII CC_GC'GG
Cuts at: 347 462 347
Size: 115 4276
SalI G'TCGA_C
Cuts at: 1428 1428
Size: 4391
SapI GCTCTTCn'nnn_
Cuts at: 2414 2624 2414
Size: 210 4181
SbfI CC_TGCA'GG
Cuts at: 1438 1438
Size: 4391
ScaI AGT'ACT
Cuts at: 3908 3908
Size: 4391
SfcI C'TryA_G
Cuts at: 33 507 773 1237 1434 2119 3087 3278 33
Size: 474 266 464 197 685 968 191 1146
Fragments arranged by size:
1146 968 685 474 464 266 197 191
SfoI GGC'GCC
Cuts at: 351 369 527 1823 2071 351
Size: 18 158 1296 248 2671
Fragments arranged by size:
2671 1296 248 158 18
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
SgrAI Cr'CCGG_yG
Cuts at: 352 532 352
Size: 180 4211
SmaI CCC'GGG
Cuts at: 272 521 1413 272
Size: 249 892 3250
Fragments arranged by size:
3250 892 249
SmlI C'TyrA_G
Cuts at: 406 1546 2461 3175 3437 406
Size: 1140 915 714 262 1360
Fragments arranged by size:
1360 1140 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4391
SphI G_CATG'C
Cuts at: 1444 1944 1444
Size: 500 3891
SrfI GCCC'GGGC
Cuts at: 272 272
Size: 4391
StyI C'CwwG_G
Cuts at: 1525 1879 1525
Size: 354 4037
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 869 1089 1974 2107 2566 2775 869
Size: 220 885 133 459 209 2485
Fragments arranged by size:
2485 885 459 220 209 133
TatI w'GTAC_w
Cuts at: 1103 3906 1103
Size: 2803 1588
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 141 173 1081 1113 3908 3940 141
Size: 32 908 32 2795 32 592
Fragments arranged by size:
2795 908 592 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2188 2188
Size: 4391
XbaI T'CTAG_A
Cuts at: 1422 1422
Size: 4391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 750 1170 1886 750
Size: 420 716 3255
Fragments arranged by size:
3255 716 420
XmaI C'CCGG_G
Cuts at: 270 519 1411 270
Size: 249 892 3250
Fragments arranged by size:
3250 892 249
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI SrfI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BsiWI
BspEI BstXI BstZ17I ClaI CspCI FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PmlI PpuMI PshAI PsiI PspXI PsrI SanDI
SexAI SfiI SnaBI SspI StuI SwaI XhoI XmnI
ZraI