Restriction map for
pF1KB7021
(Circular) (Six-base) MAPSORT of: pF1KB7021.seq Check: 9443 from: 1 to: 4757
pF1KB7021 4757 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 455 455
Size: 4757
AccI GT'mk_AC
Cuts at: 1795 1795
Size: 4757
Acc65I G'GTAC_C
Cuts at: 1465 1773 1465
Size: 308 4449
AclI AA'CG_TT
Cuts at: 4597 4597
Size: 4757
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 2582 3014 3367 4029 907
Size: 1675 432 353 662 1635
Fragments arranged by size:
1675 1635 662 432 353
AfeI AGC'GCT
Cuts at: 195 195
Size: 4757
AflIII A'CryG_T
Cuts at: 2109 3909 4190 2109
Size: 1800 281 2676
Fragments arranged by size:
2676 1800 281
AleI CACnn'nnGTG
Cuts at: 679 1394 679
Size: 715 4042
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 969 969
Size: 4757
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 340 372 340
Size: 32 4725
AlwNI CAG_nnn'CTG
Cuts at: 558 738 792 875 1215 1491 1629 1716 2062
Size: 180 54 83 340 276 138 87 346
Cuts at: 2062 3500 558
Size: 1438 1815
Fragments arranged by size:
1815 1438 346 340 276 180 138 87
83 54
ApaI G_GGCC'C
Cuts at: 136 136
Size: 4757
ApaLI G'TGCA_C
Cuts at: 949 3595 949
Size: 2646 2111
ApoI r'AATT_y
Cuts at: 1761 4110 4366 1761
Size: 2349 256 2152
Fragments arranged by size:
2349 2152 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4757
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4757
AvaI C'yCGr_G
Cuts at: 135 1499 1646 1777 135
Size: 1364 147 131 3115
Fragments arranged by size:
3115 1364 147 131
BamHI G'GATC_C
Cuts at: 1032 1782 1032
Size: 750 4007
BanI G'GyrC_C
Cuts at: 279 684 1162 1184 1226 1299 1365 1465 1773
Size: 405 478 22 42 73 66 100 308
Cuts at: 1773 2187 2435 2470 279
Size: 414 248 35 2566
Fragments arranged by size:
2566 478 414 405 308 248 100 73
66 42 35 22
BanII G_rGCy'C
Cuts at: 136 821 1507 1771 2801 136
Size: 685 686 264 1030 2092
Fragments arranged by size:
2092 1030 686 685 264
BbeI G_GCGC'C
Cuts at: 1166 2191 2439 1166
Size: 1025 248 3484
Fragments arranged by size:
3484 1025 248
BbvCI CC'TCA_GC
Cuts at: 1423 1423
Size: 4757
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1627 1661 1627
Size: 34 4723
BciVI GTATCCnnnnn_n'
Cuts at: 2655 3711 4757 2655
Size: 1056 1046 2655
Fragments arranged by size:
2655 1056 1046
BclI T'GATC_A
Cuts at: 2277 2277
Size: 4757
BglII A'GATC_T
Cuts at: 1580 2272 4309 1580
Size: 692 2037 2028
Fragments arranged by size:
2037 2028 692
BlpI GC'TnA_GC
Cuts at: 815 1869 815
Size: 1054 3703
Bme1580I G_kGCm'C
Cuts at: 136 284 486 953 2382 2475 3599 136
Size: 148 202 467 1429 93 1124 1294
Fragments arranged by size:
1429 1294 1124 467 202 148 93
BmrI ACTGGGnnnn_n'
Cuts at: 1038 1564 1969 2050 2385 4529 1038
Size: 526 405 81 335 2144 1266
Fragments arranged by size:
2144 1266 526 405 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 324 3124 324
Size: 2800 1957
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 816 1264 816
Size: 448 4309
Bpu10I CC'TnA_GC
Cuts at: 1423 1423
Size: 4757
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 1933 2812 3526 3824 496
Size: 1437 879 714 298 1429
Fragments arranged by size:
1437 1429 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4461 4461
Size: 4757
BsaAI yAC'GTr
Cuts at: 987 2110 987
Size: 1123 3634
BsaBI GATnn'nnATC
Cuts at: 6 2271 4308 6
Size: 2265 2037 455
Fragments arranged by size:
2265 2037 455
BsaHI Gr'CG_yC
Cuts at: 214 1163 1676 2188 2436 3138 4634 214
Size: 949 513 512 248 702 1496 337
Fragments arranged by size:
1496 949 702 513 512 337 248
BsaWI w'CCGG_w
Cuts at: 1946 2467 3145 3556 3703 3977 1946
Size: 521 678 411 147 274 2726
Fragments arranged by size:
2726 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1634 1634
Size: 4757
BseYI C'CCAG_C
Cuts at: 229 801 857 933 998 1457 1669 1679 1696
Size: 572 56 76 65 459 212 10 17
Cuts at: 1696 2182 2940 3605 4013 229
Size: 486 758 665 408 973
Fragments arranged by size:
973 758 665 572 486 459 408 212
76 65 56 17 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 861 1201 1504 1657 751
Size: 110 340 303 153 3851
Fragments arranged by size:
3851 340 303 153 110
BsiEI CG_ry'CG
Cuts at: 104 1404 2299 2345 3575 104
Size: 1300 895 46 1230 1286
Fragments arranged by size:
1300 1286 1230 895 46
BsiHKAI G_wGCw'C
Cuts at: 725 953 1771 2549 2739 3599 725
Size: 228 818 778 190 860 1883
Fragments arranged by size:
1883 860 818 778 228 190
BsmI GAATG_Cn'
Cuts at: 330 330
Size: 4757
Bsp1286I G_dGCh'C
Cuts at: 136 284 486 725 821 953 1507 1771 2382
Size: 148 202 239 96 132 554 264 611
Cuts at: 2382 2475 2549 2739 2801 3599 136
Size: 93 74 190 62 798 1294
Fragments arranged by size:
1294 798 611 554 264 239 202 190
148 132 96 93 74 62
BspHI T'CATG_A
Cuts at: 3189 3189
Size: 4757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 299 455 557 614 746 1807 2021 2323 2704
Size: 156 102 57 132 1061 214 302 381
Cuts at: 2704 299
Size: 2352
Fragments arranged by size:
2352 1061 381 302 214 156 132 102
57
BsrBI CCG'CTC
Cuts at: 50 906 3049 3103 4587 4752 50
Size: 856 2143 54 1484 165 55
Fragments arranged by size:
2143 1484 856 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2669 2669
Size: 4757
BsrFI r'CCGG_y
Cuts at: 1399 2755 2936 1399
Size: 1356 181 3220
Fragments arranged by size:
3220 1356 181
BssSI C'ACGA_G
Cuts at: 3028 3736 3028
Size: 708 4049
BstAPI GCAn_nnn'nTGC
Cuts at: 558 729 738 1083 1491 2134 558
Size: 171 9 345 408 643 3181
Fragments arranged by size:
3181 643 408 345 171 9
BstBI TT'CG_AA
Cuts at: 3118 3118
Size: 4757
BstEII G'GTnAC_C
Cuts at: 1356 1356
Size: 4757
BstXI CCAn_nnnn'nTGG
Cuts at: 1006 1006
Size: 4757
BstYI r'GATC_y
Cuts at: 1 341 1032 1448 1580 1740 1782 2249 2272
Size: 340 691 416 132 160 42 467 23
Cuts at: 2272 2607 2853 3159 3171 3257 4309 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 691 467 449 416 340 335 306
246 160 132 86 42 23 12
BtgI C'CryG_G
Cuts at: 107 677 1139 1270 107
Size: 570 462 131 3594
Fragments arranged by size:
3594 570 462 131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2707 2888 3051 2707
Size: 181 163 4413
Fragments arranged by size:
4413 181 163
BtsI GCAGTG_nn'
Cuts at: 556 812 951 953 1213 1566 2000 556
Size: 256 139 2 260 353 434 3313
Fragments arranged by size:
3313 434 353 260 256 139 2
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 756 791 756
Size: 35 4722
DraI TTT'AAA
Cuts at: 1756 1756
Size: 4757
DraIII CAC_nnn'GTG
Cuts at: 729 1734 729
Size: 1005 3752
DrdI GACnn_nn'nnGTC
Cuts at: 2463 3807 2463
Size: 1344 3413
EaeI y'GGCC_r
Cuts at: 536 1401 1536 2342 2516 2907 2934 4279 536
Size: 865 135 806 174 391 27 1345 1014
Fragments arranged by size:
1345 1014 865 806 391 174 135 27
EagI C'GGCC_G
Cuts at: 1401 2342 1401
Size: 941 3816
EarI CTCTTCn'nnn_
Cuts at: 463 2780 2990 463
Size: 2317 210 2230
Fragments arranged by size:
2317 2230 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2965 3707 3853 4567 2965
Size: 742 146 714 3155
Fragments arranged by size:
3155 742 714 146
EcoICRI GAG'CTC
Cuts at: 1769 1769
Size: 4757
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 816 907 1264 2582 3014 3367 4029 816
Size: 91 357 1318 432 353 662 1544
Fragments arranged by size:
1544 1318 662 432 357 353 91
EcoO109I rG'GnC_Cy
Cuts at: 132 203 735 1488 1896 132
Size: 71 532 753 408 2993
Fragments arranged by size:
2993 753 532 408 71
EcoRI G'AATT_C
Cuts at: 1761 1761
Size: 4757
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 516 2776 516
Size: 2260 2497
FspI TGC'GCA
Cuts at: 1087 2538 1087
Size: 1451 3306
HaeII r_GCGC'y
Cuts at: 157 197 1166 2191 2439 3669 4440 157
Size: 40 969 1025 248 1230 771 474
Fragments arranged by size:
1230 1025 969 771 474 248 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2599 2629 2631 2661 2599
Size: 30 2 30 4695
Fragments arranged by size:
4695 30 30 2
HincII GTy'rAC
Cuts at: 1796 1796
Size: 4757
KasI G'GCGC_C
Cuts at: 1162 2187 2435 1162
Size: 1025 248 3484
Fragments arranged by size:
3484 1025 248
KpnI G_GTAC'C
Cuts at: 1469 1777 1469
Size: 308 4449
MluI A'CGCG_T
Cuts at: 4190 4190
Size: 4757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 422 3516 3700 422
Size: 3094 184 1479
Fragments arranged by size:
3094 1479 184
MscI TGG'CCA
Cuts at: 538 2518 538
Size: 1980 2777
MslI CAynn'nnrTG
Cuts at: 679 1190 1394 1735 2746 2873 679
Size: 511 204 341 1011 127 2563
Fragments arranged by size:
2563 1011 511 341 204 127
MspA1I CmG'CkG
Cuts at: 233 372 744 981 1002 1181 1868 2182 2542
Size: 139 372 237 21 179 687 314 360
Cuts at: 2542 3326 3571 233
Size: 784 245 1419
Fragments arranged by size:
1419 784 687 372 360 314 245 237
179 139 21
NaeI GCC'GGC
Cuts at: 1401 2938 1401
Size: 1537 3220
NarI GG'CG_CC
Cuts at: 1163 2188 2436 1163
Size: 1025 248 3484
Fragments arranged by size:
3484 1025 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4757
NgoMIV G'CCGG_C
Cuts at: 1399 2936 1399
Size: 1537 3220
NspI r_CATG'y
Cuts at: 1665 1810 2310 3913 1665
Size: 145 500 1603 2509
Fragments arranged by size:
2509 1603 500 145
PasI CC'CwG_GG
Cuts at: 1347 1347
Size: 4757
PciI A'CATG_T
Cuts at: 3909 3909
Size: 4757
PflMI CCAn_nnn'nTGG
Cuts at: 792 1629 792
Size: 837 3920
PfoI T'CCnGG_A
Cuts at: 344 716 1622 344
Size: 372 906 3479
Fragments arranged by size:
3479 906 372
PmeI GTTT'AAAC
Cuts at: 1756 1756
Size: 4757
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 280 312 3203 3235 280
Size: 32 2891 32 1802
Fragments arranged by size:
2891 1802 32 32
PpuMI rG'GwC_Cy
Cuts at: 735 735
Size: 4757
PshAI GACnn'nnGTC
Cuts at: 513 513
Size: 4757
PspOMI G'GGCC_C
Cuts at: 132 132
Size: 4757
PstI C_TGCA'G
Cuts at: 380 452 554 948 1520 1804 2489 380
Size: 72 102 394 572 284 685 2648
Fragments arranged by size:
2648 685 572 394 284 102 72
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4757
PvuII CAG'CTG
Cuts at: 233 981 1002 2182 2542 233
Size: 748 21 1180 360 2448
Fragments arranged by size:
2448 1180 748 360 21
RsrII CG'GwC_CG
Cuts at: 2952 2952
Size: 4757
SacI G_AGCT'C
Cuts at: 1771 1771
Size: 4757
SalI G'TCGA_C
Cuts at: 1794 1794
Size: 4757
SapI GCTCTTCn'nnn_
Cuts at: 2780 2990 2780
Size: 210 4547
SbfI CC_TGCA'GG
Cuts at: 1804 1804
Size: 4757
ScaI AGT'ACT
Cuts at: 4274 4274
Size: 4757
SfcI C'TryA_G
Cuts at: 33 376 448 550 944 1516 1800 2485 3453
Size: 343 72 102 394 572 284 685 968
Cuts at: 3453 3644 33
Size: 191 1146
Fragments arranged by size:
1146 968 685 572 394 343 284 191
102 72
SfoI GGC'GCC
Cuts at: 1164 2189 2437 1164
Size: 1025 248 3484
Fragments arranged by size:
3484 1025 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4757
SmaI CCC'GGG
Cuts at: 137 1501 1779 137
Size: 1364 278 3115
Fragments arranged by size:
3115 1364 278
SmlI C'TyrA_G
Cuts at: 511 1912 2827 3541 3803 511
Size: 1401 915 714 262 1465
Fragments arranged by size:
1465 1401 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4757
SphI G_CATG'C
Cuts at: 1810 2310 1810
Size: 500 4257
StyI C'CwwG_G
Cuts at: 107 1154 1891 2245 107
Size: 1047 737 354 2619
Fragments arranged by size:
2619 1047 737 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 702 1342 1522 1591 2340 2473 2932 3141 702
Size: 640 180 69 749 133 459 209 2318
Fragments arranged by size:
2318 749 640 459 209 180 133 69
TatI w'GTAC_w
Cuts at: 4272 4272
Size: 4757
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4274 4306 4274
Size: 32 4725
Tth111I GACn'n_nGTC
Cuts at: 2554 2554
Size: 4757
XbaI T'CTAG_A
Cuts at: 1788 1788
Size: 4757
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 678 2252 678
Size: 1574 3183
XmaI C'CCGG_G
Cuts at: 135 1499 1777 135
Size: 1364 278 3115
Fragments arranged by size:
3115 1364 278
XmnI GAAnn'nnTTC
Cuts at: 1195 1195
Size: 4757
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AscI AvrII BaeI BbsI
BfrBI BglI BmgBI BmtI BsaXI BsiWI BsmBI BspEI
BsrGI BssHII BstZ17I Bsu36I ClaI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI