Restriction map for
pF1KB7019
(Circular) (Six-base) MAPSORT of: pF1KB7019.seq Check: 2684 from: 1 to: 5366
pF1KB7019 5366 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1393 2404 1393
Size: 1011 4355
Acc65I G'GTAC_C
Cuts at: 2382 2382
Size: 5366
AclI AA'CG_TT
Cuts at: 5206 5206
Size: 5366
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 3191 3623 3976 4638 225
Size: 2966 432 353 662 953
Fragments arranged by size:
2966 953 662 432 353
AflIII A'CryG_T
Cuts at: 786 2718 4518 4799 786
Size: 1932 1800 281 1353
Fragments arranged by size:
1932 1800 1353 281
AhdI GACnn_n'nnGTC
Cuts at: 2127 2127
Size: 5366
AleI CACnn'nnGTG
Cuts at: 440 440
Size: 5366
AlwNI CAG_nnn'CTG
Cuts at: 694 2671 4109 694
Size: 1977 1438 1951
Fragments arranged by size:
1977 1951 1438
ApaI G_GGCC'C
Cuts at: 1184 1184
Size: 5366
ApaLI G'TGCA_C
Cuts at: 4204 4204
Size: 5366
ApoI r'AATT_y
Cuts at: 1214 1577 2370 4719 4975 1214
Size: 363 793 2349 256 1605
Fragments arranged by size:
2349 1605 793 363 256
AseI AT'TA_AT
Cuts at: 20 280 20
Size: 260 5106
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5366
AvaI C'yCGr_G
Cuts at: 1053 2386 1053
Size: 1333 4033
BamHI G'GATC_C
Cuts at: 2391 2391
Size: 5366
BanI G'GyrC_C
Cuts at: 2382 2796 3044 3079 2382
Size: 414 248 35 4669
Fragments arranged by size:
4669 414 248 35
BanII G_rGCy'C
Cuts at: 1184 1523 2380 3410 1184
Size: 339 857 1030 3140
Fragments arranged by size:
3140 1030 857 339
BbeI G_GCGC'C
Cuts at: 2800 3048 2800
Size: 248 5118
BbsI GAAGACnn'nnnn_
Cuts at: 998 1767 998
Size: 769 4597
BciVI GTATCCnnnnn_n'
Cuts at: 3264 4320 5366 3264
Size: 1056 1046 3264
Fragments arranged by size:
3264 1056 1046
BclI T'GATC_A
Cuts at: 641 738 2886 641
Size: 97 2148 3121
Fragments arranged by size:
3121 2148 97
BglII A'GATC_T
Cuts at: 2881 4918 2881
Size: 2037 3329
BlpI GC'TnA_GC
Cuts at: 2478 2478
Size: 5366
Bme1580I G_kGCm'C
Cuts at: 1184 1887 2991 3084 4208 1184
Size: 703 1104 93 1124 2342
Fragments arranged by size:
2342 1124 1104 703 93
BmrI ACTGGGnnnn_n'
Cuts at: 1751 2578 2659 2994 5138 1751
Size: 827 81 335 2144 1979
Fragments arranged by size:
2144 1979 827 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 675 3733 675
Size: 3058 2308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1549 1897 1549
Size: 348 5018
Bpu10I CC'TnA_GC
Cuts at: 821 821
Size: 5366
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2542 3421 4135 4433 2542
Size: 879 714 298 3475
Fragments arranged by size:
3475 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5070 5070
Size: 5366
BsaAI yAC'GTr
Cuts at: 2719 2719
Size: 5366
BsaBI GATnn'nnATC
Cuts at: 6 290 2880 4917 6
Size: 284 2590 2037 455
Fragments arranged by size:
2590 2037 455 284
BsaHI Gr'CG_yC
Cuts at: 2797 3045 3747 5243 2797
Size: 248 702 1496 2920
Fragments arranged by size:
2920 1496 702 248
BsaWI w'CCGG_w
Cuts at: 116 299 2555 3076 3754 4165 4312 4586 116
Size: 183 2256 521 678 411 147 274 896
Fragments arranged by size:
2256 896 678 521 411 274 183 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1973 2003 1973
Size: 30 5336
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 558 664 1262 1546 1767 558
Size: 106 598 284 221 4157
Fragments arranged by size:
4157 598 284 221 106
BseYI C'CCAG_C
Cuts at: 176 2791 3549 4214 4622 176
Size: 2615 758 665 408 920
Fragments arranged by size:
2615 920 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1624 1864 1624
Size: 240 5126
BsiEI CG_ry'CG
Cuts at: 104 2908 2954 4184 104
Size: 2804 46 1230 1286
Fragments arranged by size:
2804 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 871 979 1523 2380 3158 3348 4208 871
Size: 108 544 857 778 190 860 2029
Fragments arranged by size:
2029 860 857 778 544 190 108
BsmI GAATG_Cn'
Cuts at: 112 412 729 2194 112
Size: 300 317 1465 3284
Fragments arranged by size:
3284 1465 317 300
Bsp1286I G_dGCh'C
Cuts at: 871 979 1184 1523 1887 2380 2991 3084 3158
Size: 108 205 339 364 493 611 93 74
Cuts at: 3158 3348 3410 4208 871
Size: 190 62 798 2029
Fragments arranged by size:
2029 798 611 493 364 339 205 190
108 93 74 62
BspEI T'CCGG_A
Cuts at: 116 116
Size: 5366
BspHI T'CATG_A
Cuts at: 566 3798 566
Size: 3232 2134
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2416 2630 2932 3313 2416
Size: 214 302 381 4469
Fragments arranged by size:
4469 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2000 3658 3712 5196 5361 50
Size: 1950 1658 54 1484 165 55
Fragments arranged by size:
1950 1658 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2036 3278 2036
Size: 1242 4124
BsrFI r'CCGG_y
Cuts at: 3364 3545 3364
Size: 181 5185
BsrGI T'GTAC_A
Cuts at: 1328 1328
Size: 5366
BssSI C'ACGA_G
Cuts at: 3637 4345 3637
Size: 708 4658
BstAPI GCAn_nnn'nTGC
Cuts at: 2743 2743
Size: 5366
BstBI TT'CG_AA
Cuts at: 705 3727 705
Size: 3022 2344
BstXI CCAn_nnnn'nTGG
Cuts at: 1657 1875 1657
Size: 218 5148
BstYI r'GATC_y
Cuts at: 1 169 426 2391 2858 2881 3216 3462 3768
Size: 168 257 1965 467 23 335 246 306
Cuts at: 3768 3780 3866 4918 1
Size: 12 86 1052 449
Fragments arranged by size:
1965 1052 467 449 335 306 257 246
168 86 23 12
BstZ17I GTA'TAC
Cuts at: 1394 1394
Size: 5366
BtgI C'CryG_G
Cuts at: 107 2134 107
Size: 2027 3339
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3316 3497 3660 3316
Size: 181 163 5022
Fragments arranged by size:
5022 181 163
BtsI GCAGTG_nn'
Cuts at: 2031 2609 2031
Size: 578 4788
DraI TTT'AAA
Cuts at: 2365 2365
Size: 5366
DrdI GACnn_nn'nnGTC
Cuts at: 3072 4416 3072
Size: 1344 4022
EaeI y'GGCC_r
Cuts at: 379 1713 2951 3125 3516 3543 4888 379
Size: 1334 1238 174 391 27 1345 857
Fragments arranged by size:
1345 1334 1238 857 391 174 27
EagI C'GGCC_G
Cuts at: 2951 2951
Size: 5366
EarI CTCTTCn'nnn_
Cuts at: 3389 3599 3389
Size: 210 5156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3574 4316 4462 5176 3574
Size: 742 146 714 3764
Fragments arranged by size:
3764 742 714 146
EcoICRI GAG'CTC
Cuts at: 1521 2378 1521
Size: 857 4509
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 1549 1897 3191 3623 3976 4638 225
Size: 1324 348 1294 432 353 662 953
Fragments arranged by size:
1324 1294 953 662 432 353 348
EcoO109I rG'GnC_Cy
Cuts at: 1180 1692 2307 2505 1180
Size: 512 615 198 4041
Fragments arranged by size:
4041 615 512 198
EcoRI G'AATT_C
Cuts at: 1214 2370 1214
Size: 1156 4210
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 846 2097 3385 846
Size: 1251 1288 2827
Fragments arranged by size:
2827 1288 1251
FspI TGC'GCA
Cuts at: 3147 3147
Size: 5366
HaeII r_GCGC'y
Cuts at: 509 2800 3048 4278 5049 509
Size: 2291 248 1230 771 826
Fragments arranged by size:
2291 1230 826 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 459 491 1188 1220 1427 1459 1979 2011 2113
Size: 32 697 32 207 32 520 32 102
Cuts at: 2113 2145 3208 3238 3240 3270 459
Size: 32 1063 30 2 30 2555
Fragments arranged by size:
2555 1063 697 520 207 102 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 939 2405 939
Size: 1466 3900
HindIII A'AGCT_T
Cuts at: 362 362
Size: 5366
KasI G'GCGC_C
Cuts at: 2796 3044 2796
Size: 248 5118
KpnI G_GTAC'C
Cuts at: 2386 2386
Size: 5366
MluI A'CGCG_T
Cuts at: 4799 4799
Size: 5366
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 117 2302 4125 4309 117
Size: 2185 1823 184 1174
Fragments arranged by size:
2185 1823 1174 184
MscI TGG'CCA
Cuts at: 381 1715 3127 381
Size: 1334 1412 2620
Fragments arranged by size:
2620 1412 1334
MslI CAynn'nnrTG
Cuts at: 440 488 1013 1655 3355 3482 440
Size: 48 525 642 1700 127 2324
Fragments arranged by size:
2324 1700 642 525 127 48
MspA1I CmG'CkG
Cuts at: 1194 1646 1834 2477 2791 3151 3935 4180 1194
Size: 452 188 643 314 360 784 245 2380
Fragments arranged by size:
2380 784 643 452 360 314 245 188
NaeI GCC'GGC
Cuts at: 3547 3547
Size: 5366
NarI GG'CG_CC
Cuts at: 2797 3045 2797
Size: 248 5118
NcoI C'CATG_G
Cuts at: 107 107
Size: 5366
NgoMIV G'CCGG_C
Cuts at: 3545 3545
Size: 5366
NspI r_CATG'y
Cuts at: 790 2419 2919 4522 790
Size: 1629 500 1603 1634
Fragments arranged by size:
1634 1629 1603 500
PciI A'CATG_T
Cuts at: 786 4518 786
Size: 3732 1634
PfoI T'CCnGG_A
Cuts at: 1242 1242
Size: 5366
PmeI GTTT'AAAC
Cuts at: 2365 2365
Size: 5366
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 3812
Size: 32 5334
PpuMI rG'GwC_Cy
Cuts at: 1692 1692
Size: 5366
PspOMI G'GGCC_C
Cuts at: 1180 1180
Size: 5366
PstI C_TGCA'G
Cuts at: 2413 3098 2413
Size: 685 4681
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5366
PvuII CAG'CTG
Cuts at: 1194 1834 2791 3151 1194
Size: 640 957 360 3409
Fragments arranged by size:
3409 957 640 360
RsrII CG'GwC_CG
Cuts at: 3561 3561
Size: 5366
SacI G_AGCT'C
Cuts at: 1523 2380 1523
Size: 857 4509
SalI G'TCGA_C
Cuts at: 2403 2403
Size: 5366
SanDI GG'GwC_CC
Cuts at: 1692 1692
Size: 5366
SapI GCTCTTCn'nnn_
Cuts at: 3389 3599 3389
Size: 210 5156
SbfI CC_TGCA'GG
Cuts at: 2413 2413
Size: 5366
ScaI AGT'ACT
Cuts at: 4883 4883
Size: 5366
SfcI C'TryA_G
Cuts at: 33 267 2042 2409 3094 4062 4253 33
Size: 234 1775 367 685 968 191 1146
Fragments arranged by size:
1775 1146 968 685 367 234 191
SfoI GGC'GCC
Cuts at: 2798 3046 2798
Size: 248 5118
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5366
SmaI CCC'GGG
Cuts at: 2388 2388
Size: 5366
SmlI C'TyrA_G
Cuts at: 2521 3436 4150 4412 2521
Size: 915 714 262 3475
Fragments arranged by size:
3475 915 714 262
SpeI A'CTAG_T
Cuts at: 67 1065 67
Size: 998 4368
SphI G_CATG'C
Cuts at: 2419 2919 2419
Size: 500 4866
SspI AAT'ATT
Cuts at: 342 342
Size: 5366
StuI AGG'CCT
Cuts at: 1359 1359
Size: 5366
StyI C'CwwG_G
Cuts at: 107 1277 2019 2500 2854 107
Size: 1170 742 481 354 2619
Fragments arranged by size:
2619 1170 742 481 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1275 2949 3082 3541 3750 1275
Size: 1674 133 459 209 2891
Fragments arranged by size:
2891 1674 459 209 133
TatI w'GTAC_w
Cuts at: 523 827 1328 4881 523
Size: 304 501 3553 1008
Fragments arranged by size:
3553 1008 501 304
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 316 348 776 808 1100 1132 1973 2005 4883
Size: 32 428 32 292 32 841 32 2878
Cuts at: 4883 4915 316
Size: 32 767
Fragments arranged by size:
2878 841 767 428 292 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3163 3163
Size: 5366
XbaI T'CTAG_A
Cuts at: 2397 2397
Size: 5366
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2861 2861
Size: 5366
XmaI C'CCGG_G
Cuts at: 2386 2386
Size: 5366
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BsiWI BsmBI BssHII BstEII Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI XmnI ZraI