Restriction map for
pF1KB7017
(Circular) (Six-base) MAPSORT of: pF1KB7017.seq Check: 9216 from: 1 to: 5777
pF1KB7017 5777 bp
With 182 enzymes: SgfI *
August 12, 2008 14:28 ..
AatII G_ACGT'C
Cuts at: 958 1513 958
Size: 555 5222
AccI GT'mk_AC
Cuts at: 830 2815 830
Size: 1985 3792
Acc65I G'GTAC_C
Cuts at: 404 2793 404
Size: 2389 3388
AclI AA'CG_TT
Cuts at: 5617 5617
Size: 5777
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 985 1938 3602 4034 4781 5049 985
Size: 953 1664 432 747 268 1713
Fragments arranged by size:
1713 1664 953 747 432 268
AflIII A'CryG_T
Cuts at: 449 1805 2648 3129 4233 5210 449
Size: 1356 843 481 1104 977 1016
Fragments arranged by size:
1356 1104 1016 977 843 481
AgeI A'CCGG_T
Cuts at: 4165 4165
Size: 5777
AleI CACnn'nnGTG
Cuts at: 1036 1036
Size: 5777
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1280 1280
Size: 5777
AlwNI CAG_nnn'CTG
Cuts at: 691 1599 3082 4649 691
Size: 908 1483 1567 1819
Fragments arranged by size:
1819 1567 1483 908
ApaI G_GGCC'C
Cuts at: 266 1749 2204 2302 2382 2527 2611 266
Size: 1483 455 98 80 145 84 3432
Fragments arranged by size:
3432 1483 455 145 98 84 80
ApaLI G'TGCA_C
Cuts at: 4547 4547
Size: 5777
ApoI r'AATT_y
Cuts at: 2781 5130 5386 2781
Size: 2349 256 3172
Fragments arranged by size:
3172 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5777
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5777
AvaI C'yCGr_G
Cuts at: 736 899 1048 1180 1231 1249 1391 1695 1776
Size: 163 149 132 51 18 142 304 81
Cuts at: 1776 2092 2217 2236 2375 2383 2797 736
Size: 316 125 19 139 8 414 3716
Fragments arranged by size:
3716 414 316 304 163 149 142 139
132 125 81 51 19 18 8
BamHI G'GATC_C
Cuts at: 1358 2802 1358
Size: 1444 4333
BanI G'GyrC_C
Cuts at: 172 404 1200 1215 1332 1379 1780 2122 2151
Size: 232 796 15 117 47 401 342 29
Cuts at: 2151 2367 2793 3207 3455 3490 172
Size: 216 426 414 248 35 2459
Fragments arranged by size:
2459 796 426 414 401 342 248 232
216 117 47 35 29 15
BanII G_rGCy'C
Cuts at: 266 383 1520 1749 2204 2302 2382 2527 2575
Size: 117 1137 229 455 98 80 145 48
Cuts at: 2575 2611 2791 3821 266
Size: 36 180 1030 2222
Fragments arranged by size:
2222 1137 1030 455 229 180 145 117
98 80 48 36
BbeI G_GCGC'C
Cuts at: 176 2126 2155 2371 3211 3459 176
Size: 1950 29 216 840 248 2494
Fragments arranged by size:
2494 1950 840 248 216 29
BbsI GAAGACnn'nnnn_
Cuts at: 1434 1518 1434
Size: 84 5693
BbvCI CC'TCA_GC
Cuts at: 1771 2541 1771
Size: 770 5007
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5743
BciVI GTATCCnnnnn_n'
Cuts at: 3675 4436 5777 3675
Size: 761 1341 3675
Fragments arranged by size:
3675 1341 761
BclI T'GATC_A
Cuts at: 3297 3297
Size: 5777
BglI GCCn_nnn'nGGC
Cuts at: 1052 1424 2026 2069 2149 2623 2690 1052
Size: 372 602 43 80 474 67 4139
Fragments arranged by size:
4139 602 474 372 80 67 43
BglII A'GATC_T
Cuts at: 427 3292 5329 427
Size: 2865 2037 875
Fragments arranged by size:
2865 2037 875
BlpI GC'TnA_GC
Cuts at: 208 1603 2053 2889 208
Size: 1395 450 836 3096
Fragments arranged by size:
3096 1395 836 450
Bme1580I G_kGCm'C
Cuts at: 266 1337 1749 1783 2204 2302 2382 2527 2611
Size: 1071 412 34 421 98 80 145 84
Cuts at: 2611 2713 2771 3402 3495 4551 266
Size: 102 58 631 93 1056 1492
Fragments arranged by size:
1492 1071 1056 631 421 412 145 102
98 93 84 80 58 34
BmgBI CAC'GTC
Cuts at: 389 918 1407 1806 389
Size: 529 489 399 4360
Fragments arranged by size:
4360 529 489 399
BmrI ACTGGGnnnn_n'
Cuts at: 175 2614 2989 3070 3405 5549 175
Size: 2439 375 81 335 2144 403
Fragments arranged by size:
2439 2144 403 375 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4144 4144
Size: 5777
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 444 892 961 1254 1552 2589 397
Size: 47 448 69 293 298 1037 3585
Fragments arranged by size:
3585 1037 448 298 293 69 47
Bpu10I CC'TnA_GC
Cuts at: 1771 1830 2541 1771
Size: 59 711 5007
Fragments arranged by size:
5007 711 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1456 2953 3832 4324 4622 1456
Size: 1497 879 492 298 2611
Fragments arranged by size:
2611 1497 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1537 5481 1537
Size: 3944 1833
BsaAI yAC'GTr
Cuts at: 3130 3130
Size: 5777
BsaBI GATnn'nnATC
Cuts at: 6 3291 5328 6
Size: 3285 2037 455
Fragments arranged by size:
3285 2037 455
BsaHI Gr'CG_yC
Cuts at: 173 270 955 1195 1510 2123 2152 2245 2368
Size: 97 685 240 315 613 29 93 123
Cuts at: 2368 3208 3456 4158 5654 173
Size: 840 248 702 1496 296
Fragments arranged by size:
1496 840 702 685 613 315 296 248
240 123 97 93 29
BsaWI w'CCGG_w
Cuts at: 2966 3487 4165 4439 4586 4997 2966
Size: 521 678 274 147 411 3746
Fragments arranged by size:
3746 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 388 418 466 496 934 964 1252 1282 1928
Size: 30 48 30 438 30 288 30 646
Cuts at: 1928 1958 388
Size: 30 4207
Fragments arranged by size:
4207 646 438 288 48 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 208 1549 2610 2719 208
Size: 1341 1061 109 3266
Fragments arranged by size:
3266 1341 1061 109
BseYI C'CCAG_C
Cuts at: 436 1087 1341 2171 2535 3202 3960 4537 5033
Size: 651 254 830 364 667 758 577 496
Cuts at: 5033 436
Size: 1180
Fragments arranged by size:
1180 830 758 667 651 577 496 364
254
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1824 1824
Size: 5777
BsiEI CG_ry'CG
Cuts at: 104 1555 2424 2448 2627 2707 3319 3365 4573
Size: 1451 869 24 179 80 612 46 1208
Cuts at: 4573 104
Size: 1308
Fragments arranged by size:
1451 1308 1208 869 612 179 80 46
24
BsiHKAI G_wGCw'C
Cuts at: 2791 3569 3759 4551 2791
Size: 778 190 792 4017
Fragments arranged by size:
4017 792 778 190
BsiWI C'GTAC_G
Cuts at: 632 632
Size: 5777
Bsp1286I G_dGCh'C
Cuts at: 266 383 1337 1520 1749 1783 2204 2302 2382
Size: 117 954 183 229 34 421 98 80
Cuts at: 2382 2527 2575 2611 2713 2771 2791 3402 3495
Size: 145 48 36 102 58 20 611 93
Cuts at: 3495 3569 3759 3821 4551 266
Size: 74 190 62 730 1492
Fragments arranged by size:
1492 954 730 611 421 229 190 183
145 117 102 98 93 80 74 62
58 48 36 34 20
BspEI T'CCGG_A
Cuts at: 4997 4997
Size: 5777
BspHI T'CATG_A
Cuts at: 4953 4953
Size: 5777
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1907 2827 3041 3343 3724 1907
Size: 920 214 302 381 3960
Fragments arranged by size:
3960 920 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1986 2472 4069 4123 5607 5772 50
Size: 1936 486 1597 54 1484 165 55
Fragments arranged by size:
1936 1597 1484 486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3689 3689
Size: 5777
BsrFI r'CCGG_y
Cuts at: 1055 1197 1414 1550 2498 3775 3956 4165 1055
Size: 142 217 136 948 1277 181 209 2667
Fragments arranged by size:
2667 1277 948 217 209 181 142 136
BssSI C'ACGA_G
Cuts at: 4048 4406 4048
Size: 358 5419
BstAPI GCAn_nnn'nTGC
Cuts at: 289 1599 3154 289
Size: 1310 1555 2912
Fragments arranged by size:
2912 1555 1310
BstBI TT'CG_AA
Cuts at: 4138 4138
Size: 5777
BstYI r'GATC_y
Cuts at: 1 427 1358 2802 3269 3292 3627 3873 4885
Size: 426 931 1444 467 23 335 246 1012
Cuts at: 4885 4971 4983 5329 1
Size: 86 12 346 449
Fragments arranged by size:
1444 1012 931 467 449 426 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 191 1503 1824 2593 191
Size: 1312 321 769 3375
Fragments arranged by size:
3375 1312 769 321
BtgI C'CryG_G
Cuts at: 177 392 1061 1940 2316 2457 2519 2560 2688
Size: 215 669 879 376 141 62 41 128
Cuts at: 2688 177
Size: 3266
Fragments arranged by size:
3266 879 669 376 215 141 128 62
41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 868 3727 3908 4071 868
Size: 2859 181 163 2574
Fragments arranged by size:
2859 2574 181 163
BtsI GCAGTG_nn'
Cuts at: 955 3020 955
Size: 2065 3712
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 513 548 513
Size: 35 5742
DraI TTT'AAA
Cuts at: 2776 2776
Size: 5777
DrdI GACnn_nn'nnGTC
Cuts at: 3483 4341 3483
Size: 858 4919
EaeI y'GGCC_r
Cuts at: 1070 1552 2319 2421 2445 2624 2704 3362 3536
Size: 482 767 102 24 179 80 658 174
Cuts at: 3536 3927 3954 5299 1070
Size: 391 27 1345 1548
Fragments arranged by size:
1548 1345 767 658 482 391 179 174
102 80 27 24
EagI C'GGCC_G
Cuts at: 1552 2421 2445 2624 2704 3362 1552
Size: 869 24 179 80 658 3967
Fragments arranged by size:
3967 869 658 179 80 24
EarI CTCTTCn'nnn_
Cuts at: 667 685 3800 4010 667
Size: 18 3115 210 2434
Fragments arranged by size:
3115 2434 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2120 2761 3985 4295 4441 5587 2120
Size: 641 1224 310 146 1146 2310
Fragments arranged by size:
2310 1224 1146 641 310 146
EcoICRI GAG'CTC
Cuts at: 2789 2789
Size: 5777
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 444 892 961 985 1254 1552 1938 2589
Size: 47 448 69 24 269 298 386 651
Cuts at: 2589 3602 4034 4781 5049 397
Size: 1013 432 747 268 1125
Fragments arranged by size:
1125 1013 747 651 448 432 386 298
269 268 69 47 24
EcoNI CCTnn'n_nnAGG
Cuts at: 874 1132 1867 874
Size: 258 735 4784
Fragments arranged by size:
4784 735 258
EcoO109I rG'GnC_Cy
Cuts at: 1632 1728 1746 2089 2142 2201 2299 2379 2524
Size: 96 18 343 53 59 98 80 145
Cuts at: 2524 2608 2916 1632
Size: 84 308 4493
Fragments arranged by size:
4493 343 308 145 98 96 84 80
59 53 18
EcoRI G'AATT_C
Cuts at: 2781 2781
Size: 5777
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3796 3796
Size: 5777
FseI GG_CCGG'CC
Cuts at: 1059 1059
Size: 5777
FspI TGC'GCA
Cuts at: 757 1242 3558 757
Size: 485 2316 2976
Fragments arranged by size:
2976 2316 485
HaeII r_GCGC'y
Cuts at: 176 1277 2126 2155 2265 2371 3211 3459 4481
Size: 1101 849 29 110 106 840 248 1022
Cuts at: 4481 5460 176
Size: 979 493
Fragments arranged by size:
1101 1022 979 849 840 493 248 110
106 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 593 625 1159 1191 1299 1331 2200 2232 2524
Size: 32 534 32 108 32 869 32 292
Cuts at: 2524 2556 3619 3649 3651 3681 593
Size: 32 1063 30 2 30 2689
Fragments arranged by size:
2689 1063 869 534 292 108 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 327 2816 327
Size: 2489 3288
KasI G'GCGC_C
Cuts at: 172 2122 2151 2367 3207 3455 172
Size: 1950 29 216 840 248 2494
Fragments arranged by size:
2494 1950 840 248 216 29
KpnI G_GTAC'C
Cuts at: 408 2797 408
Size: 2389 3388
MluI A'CGCG_T
Cuts at: 5210 5210
Size: 5777
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 436 569 579 4448 4632 436
Size: 133 10 3869 184 1581
Fragments arranged by size:
3869 1581 184 133 10
MscI TGG'CCA
Cuts at: 3538 3538
Size: 5777
MslI CAynn'nnrTG
Cuts at: 448 650 1036 3766 3893 448
Size: 202 386 2730 127 2332
Fragments arranged by size:
2730 2332 386 202 127
MspA1I CmG'CkG
Cuts at: 260 614 626 1144 1386 1596 1655 2250 2562
Size: 354 12 518 242 210 59 595 312
Cuts at: 2562 2660 2690 2888 3202 3562 4575 4820 260
Size: 98 30 198 314 360 1013 245 1217
Fragments arranged by size:
1217 1013 595 518 360 354 314 312
245 242 210 198 98 59 30 12
NaeI GCC'GGC
Cuts at: 1057 1416 2500 3958 1057
Size: 359 1084 1458 2876
Fragments arranged by size:
2876 1458 1084 359
NarI GG'CG_CC
Cuts at: 173 2123 2152 2368 3208 3456 173
Size: 1950 29 216 840 248 2494
Fragments arranged by size:
2494 1950 840 248 216 29
NgoMIV G'CCGG_C
Cuts at: 1055 1414 2498 3956 1055
Size: 359 1084 1458 2876
Fragments arranged by size:
2876 1458 1084 359
NspI r_CATG'y
Cuts at: 2830 3330 4237 2830
Size: 500 907 4370
Fragments arranged by size:
4370 907 500
PasI CC'CwG_GG
Cuts at: 221 1421 2295 221
Size: 1200 874 3703
Fragments arranged by size:
3703 1200 874
PciI A'CATG_T
Cuts at: 4233 4233
Size: 5777
PflMI CCAn_nnn'nTGG
Cuts at: 810 2211 2295 810
Size: 1401 84 4292
Fragments arranged by size:
4292 1401 84
PfoI T'CCnGG_A
Cuts at: 842 1529 842
Size: 687 5090
PmeI GTTT'AAAC
Cuts at: 2776 2776
Size: 5777
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4916 4948 4916
Size: 32 5745
PpuMI rG'GwC_Cy
Cuts at: 1728 2089 1728
Size: 361 5416
PshAI GACnn'nnGTC
Cuts at: 1438 1438
Size: 5777
PspOMI G'GGCC_C
Cuts at: 262 1745 2200 2298 2378 2523 2607 262
Size: 1483 455 98 80 145 84 3432
Fragments arranged by size:
3432 1483 455 145 98 84 80
PspXI vC'TCGA_Gb
Cuts at: 1391 1391
Size: 5777
PstI C_TGCA'G
Cuts at: 593 1025 2195 2824 3509 593
Size: 432 1170 629 685 2861
Fragments arranged by size:
2861 1170 685 629 432
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5777
PvuII CAG'CTG
Cuts at: 1144 1386 3202 3562 1144
Size: 242 1816 360 3359
Fragments arranged by size:
3359 1816 360 242
RsrII CG'GwC_CG
Cuts at: 2493 3972 2493
Size: 1479 4298
SacI G_AGCT'C
Cuts at: 2791 2791
Size: 5777
SacII CC_GC'GG
Cuts at: 2563 2691 2563
Size: 128 5649
SalI G'TCGA_C
Cuts at: 2814 2814
Size: 5777
SanDI GG'GwC_CC
Cuts at: 2089 2089
Size: 5777
SapI GCTCTTCn'nnn_
Cuts at: 685 3800 4010 685
Size: 3115 210 2452
Fragments arranged by size:
3115 2452 210
SbfI CC_TGCA'GG
Cuts at: 2824 2824
Size: 5777
ScaI AGT'ACT
Cuts at: 1693 5294 1693
Size: 3601 2176
SfcI C'TryA_G
Cuts at: 33 573 589 1021 2191 2820 3505 4498 4689
Size: 540 16 432 1170 629 685 993 191
Cuts at: 4689 33
Size: 1121
Fragments arranged by size:
1170 1121 993 685 629 540 432 191
16
SfoI GGC'GCC
Cuts at: 174 2124 2153 2369 3209 3457 174
Size: 1950 29 216 840 248 2494
Fragments arranged by size:
2494 1950 840 248 216 29
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5777
SgrAI Cr'CCGG_yG
Cuts at: 1197 2498 1197
Size: 1301 4476
SmaI CCC'GGG
Cuts at: 1233 1251 1778 2094 2377 2799 1233
Size: 18 527 316 283 422 4211
Fragments arranged by size:
4211 527 422 316 283 18
SmlI C'TyrA_G
Cuts at: 1391 1471 2932 3847 4339 4601 1391
Size: 80 1461 915 492 262 2567
Fragments arranged by size:
2567 1461 915 492 262 80
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5777
SphI G_CATG'C
Cuts at: 2830 3330 2830
Size: 500 5277
SrfI GCCC'GGGC
Cuts at: 1778 2377 1778
Size: 599 5178
StyI C'CwwG_G
Cuts at: 2177 2528 2911 3265 2177
Size: 351 383 354 4689
Fragments arranged by size:
4689 383 354 351
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2659 3360 3493 3952 4161 2659
Size: 701 133 459 209 4275
Fragments arranged by size:
4275 701 459 209 133
TatI w'GTAC_w
Cuts at: 1259 1691 5292 1259
Size: 432 3601 1744
Fragments arranged by size:
3601 1744 432
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 466 498 934 966 1928 1960 5294 5326 466
Size: 32 436 32 962 32 3334 32 917
Fragments arranged by size:
3334 962 917 436 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3574 3574
Size: 5777
XbaI T'CTAG_A
Cuts at: 2808 2808
Size: 5777
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2526 3272 2526
Size: 746 5031
XhoI C'TCGA_G
Cuts at: 1391 1391
Size: 5777
XmaI C'CCGG_G
Cuts at: 1231 1249 1776 2092 2375 2797 1231
Size: 18 527 316 283 422 4211
Fragments arranged by size:
4211 527 422 316 283 18
ZraI GAC'GTC
Cuts at: 956 1511 956
Size: 555 5222
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AloI AscI AvrII BaeI
BfrBI BmtI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI DraIII EcoRV FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PmlI
PsiI PsrI SexAI SfiI SnaBI SspI StuI SwaI
XmnI