Restriction map for
pF1KB7005
(Circular) (Six-base) MAPSORT of: pF1KB7005.seq Check: 4638 from: 1 to: 6890
pF1KB7005 6890 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AatII G_ACGT'C
Cuts at: 2059 3593 2059
Size: 1534 5356
AccI GT'mk_AC
Cuts at: 1513 2365 2630 3244 3458 3680 3928 1513
Size: 852 265 614 214 222 248 4475
Fragments arranged by size:
4475 852 614 265 248 222 214
Acc65I G'GTAC_C
Cuts at: 3906 3906
Size: 6890
AclI AA'CG_TT
Cuts at: 6730 6730
Size: 6890
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1153 1784 1983 2750 2913 2925 4715 5147
Size: 681 631 199 767 163 12 1790 432
Cuts at: 5147 5894 6162 472
Size: 747 268 1200
Fragments arranged by size:
1790 1200 767 747 681 631 432 268
199 163 12
AflIII A'CryG_T
Cuts at: 2444 4242 5346 6323 2444
Size: 1798 1104 977 3011
Fragments arranged by size:
3011 1798 1104 977
AgeI A'CCGG_T
Cuts at: 3052 5278 3052
Size: 2226 4664
AhdI GACnn_n'nnGTC
Cuts at: 3672 3672
Size: 6890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1075 1877 2722 1075
Size: 802 845 5243
Fragments arranged by size:
5243 845 802
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3079 3111 3079
Size: 32 6858
AlwNI CAG_nnn'CTG
Cuts at: 1131 3211 3520 3792 4195 5762 1131
Size: 2080 309 272 403 1567 2259
Fragments arranged by size:
2259 2080 1567 403 309 272
ApaLI G'TGCA_C
Cuts at: 1050 5660 1050
Size: 4610 2280
ApoI r'AATT_y
Cuts at: 189 2737 3003 3253 3267 3894 6243 6499 189
Size: 2548 266 250 14 627 2349 256 580
Fragments arranged by size:
2548 2349 627 580 266 256 250 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 6890
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6890
AvaI C'yCGr_G
Cuts at: 2083 3524 3822 3910 2083
Size: 1441 298 88 5063
Fragments arranged by size:
5063 1441 298 88
AvrII C'CTAG_G
Cuts at: 699 699
Size: 6890
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 386 419 386
Size: 33 6857
BamHI G'GATC_C
Cuts at: 3915 3915
Size: 6890
BanI G'GyrC_C
Cuts at: 901 2275 3906 4320 4568 4603 901
Size: 1374 1631 414 248 35 3188
Fragments arranged by size:
3188 1631 1374 414 248 35
BanII G_rGCy'C
Cuts at: 273 2398 2462 2816 3904 4934 273
Size: 2125 64 354 1088 1030 2229
Fragments arranged by size:
2229 2125 1088 1030 354 64
BbeI G_GCGC'C
Cuts at: 4324 4572 4324
Size: 248 6642
BbsI GAAGACnn'nnnn_
Cuts at: 2032 2645 3801 2032
Size: 613 1156 5121
Fragments arranged by size:
5121 1156 613
BbvCI CC'TCA_GC
Cuts at: 1640 2287 1640
Size: 647 6243
BciVI GTATCCnnnnn_n'
Cuts at: 2115 4788 5549 6890 2115
Size: 2673 761 1341 2115
Fragments arranged by size:
2673 2115 1341 761
BclI T'GATC_A
Cuts at: 3362 4410 3362
Size: 1048 5842
BfrBI ATG'CAT
Cuts at: 1854 1854
Size: 6890
BglII A'GATC_T
Cuts at: 1020 1350 1557 1875 3299 4405 6442 1020
Size: 330 207 318 1424 1106 2037 1468
Fragments arranged by size:
2037 1468 1424 1106 330 318 207
BlpI GC'TnA_GC
Cuts at: 1391 2575 4002 1391
Size: 1184 1427 4279
Fragments arranged by size:
4279 1427 1184
Bme1580I G_kGCm'C
Cuts at: 1054 1236 1653 2280 3829 4515 4608 5664 1054
Size: 182 417 627 1549 686 93 1056 2280
Fragments arranged by size:
2280 1549 1056 686 627 417 182 93
BmgBI CAC'GTC
Cuts at: 2445 3864 2445
Size: 1419 5471
BmrI ACTGGGnnnn_n'
Cuts at: 2192 3483 4102 4183 4518 6662 2192
Size: 1291 619 81 335 2144 2420
Fragments arranged by size:
2420 2144 1291 619 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2250 2268 5257 2250
Size: 18 2989 3883
Fragments arranged by size:
3883 2989 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 777 1216 2149 2287 3373 3801 200
Size: 577 439 933 138 1086 428 3289
Fragments arranged by size:
3289 1086 933 577 439 428 138
Bpu10I CC'TnA_GC
Cuts at: 1640 2287 1640
Size: 647 6243
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 750 3130 3703 4066 4945 5437 5735 750
Size: 2380 573 363 879 492 298 1905
Fragments arranged by size:
2380 1905 879 573 492 363 298
BsaI GGTCTCn'nnnn_
Cuts at: 2080 6594 2080
Size: 4514 2376
BsaAI yAC'GTr
Cuts at: 1911 1971 2283 2301 2472 2730 4243 1911
Size: 60 312 18 171 258 1513 4558
Fragments arranged by size:
4558 1513 312 258 171 60 18
BsaBI GATnn'nnATC
Cuts at: 6 3038 4404 6441 6
Size: 3032 1366 2037 455
Fragments arranged by size:
3032 2037 1366 455
BsaHI Gr'CG_yC
Cuts at: 113 830 857 2056 3590 4321 4569 5271 6767
Size: 717 27 1199 1534 731 248 702 1496
Cuts at: 6767 113
Size: 236
Fragments arranged by size:
1534 1496 1199 731 717 702 248 236
27
BsaWI w'CCGG_w
Cuts at: 2021 2669 3052 4079 4600 5278 5552 5699 6110
Size: 648 383 1027 521 678 274 147 411
Cuts at: 6110 2021
Size: 2801
Fragments arranged by size:
2801 1027 678 648 521 411 383 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 750 780 2942 2972 750
Size: 30 2162 30 4668
Fragments arranged by size:
4668 2162 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 331 802 1783 2226 2284 3621 136
Size: 195 471 981 443 58 1337 3405
Fragments arranged by size:
3405 1337 981 471 443 195 58
BseYI C'CCAG_C
Cuts at: 1091 1175 1646 2462 2772 2799 3098 3204 3847
Size: 84 471 816 310 27 299 106 643
Cuts at: 3847 4315 5073 5650 6146 1091
Size: 468 758 577 496 1835
Fragments arranged by size:
1835 816 758 643 577 496 471 468
310 299 106 84 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 1096 1144 2313 2452 2455 3608 859
Size: 237 48 1169 139 3 1153 4141
Fragments arranged by size:
4141 1169 1153 237 139 48 3
BsiEI CG_ry'CG
Cuts at: 104 3800 4432 4478 5686 104
Size: 3696 632 46 1208 1308
Fragments arranged by size:
3696 1308 1208 632 46
BsiHKAI G_wGCw'C
Cuts at: 602 1054 1167 1623 3761 3904 4682 4872 5664
Size: 452 113 456 2138 143 778 190 792
Cuts at: 5664 602
Size: 1828
Fragments arranged by size:
2138 1828 792 778 456 452 190 143
113
BsmI GAATG_Cn'
Cuts at: 651 1854 2504 3738 651
Size: 1203 650 1234 3803
Fragments arranged by size:
3803 1234 1203 650
Bsp1286I G_dGCh'C
Cuts at: 273 602 1054 1167 1236 1623 1653 2280 2398
Size: 329 452 113 69 387 30 627 118
Cuts at: 2398 2462 2816 3761 3829 3904 4515 4608 4682
Size: 64 354 945 68 75 611 93 74
Cuts at: 4682 4872 4934 5664 273
Size: 190 62 730 1499
Fragments arranged by size:
1499 945 730 627 611 452 387 354
329 190 118 113 93 75 74 69
68 64 62 30
BspEI T'CCGG_A
Cuts at: 2669 6110 2669
Size: 3441 3449
BspHI T'CATG_A
Cuts at: 6066 6066
Size: 6890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1369 1730 3077 3940 4154 4456 4837 1369
Size: 361 1347 863 214 302 381 3422
Fragments arranged by size:
3422 1347 863 381 361 302 214
BsrBI CCG'CTC
Cuts at: 50 3561 5182 5236 6720 6885 50
Size: 3511 1621 54 1484 165 55
Fragments arranged by size:
3511 1621 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 670 1250 2586 3177 4802 670
Size: 580 1336 591 1625 2758
Fragments arranged by size:
2758 1625 1336 591 580
BsrFI r'CCGG_y
Cuts at: 135 2536 3052 3182 3795 3799 4888 5069 5278
Size: 2401 516 130 613 4 1089 181 209
Cuts at: 5278 135
Size: 1747
Fragments arranged by size:
2401 1747 1089 613 516 209 181 130
4
BsrGI T'GTAC_A
Cuts at: 1943 1943
Size: 6890
BssHII G'CGCG_C
Cuts at: 1095 1095
Size: 6890
BssSI C'ACGA_G
Cuts at: 2640 2694 3366 5161 5519 2640
Size: 54 672 1795 358 4011
Fragments arranged by size:
4011 1795 672 358 54
BstAPI GCAn_nnn'nTGC
Cuts at: 3792 4267 3792
Size: 475 6415
BstBI TT'CG_AA
Cuts at: 5251 5251
Size: 6890
BstEII G'GTnAC_C
Cuts at: 2859 2859
Size: 6890
BstXI CCAn_nnnn'nTGG
Cuts at: 248 975 1902 3516 248
Size: 727 927 1614 3622
Fragments arranged by size:
3622 1614 927 727
BstYI r'GATC_y
Cuts at: 1 1020 1350 1557 1875 3299 3915 4382 4405
Size: 1019 330 207 318 1424 616 467 23
Cuts at: 4405 4740 4986 5998 6084 6096 6442 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1424 1019 1012 616 467 449 346 335
330 318 246 207 86 23 12
BstZ17I GTA'TAC
Cuts at: 1514 2366 3245 1514
Size: 852 879 5159
Fragments arranged by size:
5159 879 852
Bsu36I CC'TnA_GG
Cuts at: 2253 2967 2253
Size: 714 6176
BtgI C'CryG_G
Cuts at: 107 149 1078 1901 2893 107
Size: 42 929 823 992 4104
Fragments arranged by size:
4104 992 929 823 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2901 4840 5021 5184 2901
Size: 1939 181 163 4607
Fragments arranged by size:
4607 1939 181 163
BtsI GCAGTG_nn'
Cuts at: 3485 3790 4133 3485
Size: 305 343 6242
Fragments arranged by size:
6242 343 305
ClaI AT'CG_AT
Cuts at: 991 991
Size: 6890
DraI TTT'AAA
Cuts at: 3258 3889 3258
Size: 631 6259
DraIII CAC_nnn'GTG
Cuts at: 437 1162 1263 2877 437
Size: 725 101 1614 4450
Fragments arranged by size:
4450 1614 725 101
DrdI GACnn_nn'nnGTC
Cuts at: 4596 5454 4596
Size: 858 6032
EaeI y'GGCC_r
Cuts at: 264 1682 2538 2847 3797 3801 4475 4649 5040
Size: 1418 856 309 950 4 674 174 391
Cuts at: 5040 5067 6412 264
Size: 27 1345 742
Fragments arranged by size:
1418 1345 950 856 742 674 391 309
174 27 4
EagI C'GGCC_G
Cuts at: 3797 4475 3797
Size: 678 6212
EarI CTCTTCn'nnn_
Cuts at: 627 733 868 931 1069 1760 3152 3568 4913
Size: 106 135 63 138 691 1392 416 1345
Cuts at: 4913 5123 627
Size: 210 2394
Fragments arranged by size:
2394 1392 1345 691 416 210 138 135
106 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2626 5098 5408 5554 6700 2626
Size: 2472 310 146 1146 2816
Fragments arranged by size:
2816 2472 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3902 3902
Size: 6890
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 472 777 1153 1216 1784 1983 2149 2287
Size: 272 305 376 63 568 199 166 138
Cuts at: 2287 2750 2913 2925 3373 3801 4715 5147 5894
Size: 463 163 12 448 428 914 432 747
Cuts at: 5894 6162 200
Size: 268 928
Fragments arranged by size:
928 914 747 568 463 448 432 428
376 305 272 268 199 166 163 138
63 12
EcoNI CCTnn'n_nnAGG
Cuts at: 1639 2605 3385 1639
Size: 966 780 5144
Fragments arranged by size:
5144 966 780
EcoO109I rG'GnC_Cy
Cuts at: 257 1336 4029 257
Size: 1079 2693 3118
Fragments arranged by size:
3118 2693 1079
EcoRI G'AATT_C
Cuts at: 3894 3894
Size: 6890
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4909 4909
Size: 6890
FseI GG_CCGG'CC
Cuts at: 3803 3803
Size: 6890
FspI TGC'GCA
Cuts at: 1027 4671 1027
Size: 3644 3246
HaeII r_GCGC'y
Cuts at: 4324 4572 5594 6573 4324
Size: 248 1022 979 4641
Fragments arranged by size:
4641 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 941 973 2434 2466 2688 2720 3658 3690 4732
Size: 32 1461 32 222 32 938 32 1042
Cuts at: 4732 4762 4764 4794 941
Size: 30 2 30 3037
Fragments arranged by size:
3037 1461 1042 938 222 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 882 1368 3459 3929 882
Size: 486 2091 470 3843
Fragments arranged by size:
3843 2091 486 470
HindIII A'AGCT_T
Cuts at: 1463 3439 1463
Size: 1976 4914
KasI G'GCGC_C
Cuts at: 4320 4568 4320
Size: 248 6642
KpnI G_GTAC'C
Cuts at: 3910 3910
Size: 6890
MluI A'CGCG_T
Cuts at: 6323 6323
Size: 6890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 235 629 1530 2660 2708 3174 5561 5745 235
Size: 394 901 1130 48 466 2387 184 1380
Fragments arranged by size:
2387 1380 1130 901 466 394 184 48
MscI TGG'CCA
Cuts at: 4651 4651
Size: 6890
MslI CAynn'nnrTG
Cuts at: 973 1507 3508 3514 3550 4879 5006 973
Size: 534 2001 6 36 1329 127 2857
Fragments arranged by size:
2857 2001 1329 534 127 36 6
MspA1I CmG'CkG
Cuts at: 129 1680 2379 2466 2481 2852 3027 3222 3653
Size: 1551 699 87 15 371 175 195 431
Cuts at: 3653 4001 4315 4675 5688 5933 129
Size: 348 314 360 1013 245 1086
Fragments arranged by size:
1551 1086 1013 699 431 371 360 348
314 245 195 175 87 15
NaeI GCC'GGC
Cuts at: 3797 3801 5071 3797
Size: 4 1270 5616
Fragments arranged by size:
5616 1270 4
NarI GG'CG_CC
Cuts at: 4321 4569 4321
Size: 248 6642
NcoI C'CATG_G
Cuts at: 107 149 107
Size: 42 6848
NgoMIV G'CCGG_C
Cuts at: 3795 3799 5069 3795
Size: 4 1270 5616
Fragments arranged by size:
5616 1270 4
NsiI A_TGCA'T
Cuts at: 1856 1856
Size: 6890
NspI r_CATG'y
Cuts at: 2211 2799 3943 4443 5350 2211
Size: 588 1144 500 907 3751
Fragments arranged by size:
3751 1144 907 588 500
PciI A'CATG_T
Cuts at: 5346 5346
Size: 6890
PflMI CCAn_nnn'nTGG
Cuts at: 1263 3867 1263
Size: 2604 4286
PfoI T'CCnGG_A
Cuts at: 640 3866 640
Size: 3226 3664
PmeI GTTT'AAAC
Cuts at: 3889 3889
Size: 6890
PmlI CAC'GTG
Cuts at: 1911 1971 2283 2301 2472 2730 1911
Size: 60 312 18 171 258 6071
Fragments arranged by size:
6071 312 258 171 60 18
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1121 1153 6029 6061 1121
Size: 32 4876 32 1950
Fragments arranged by size:
4876 1950 32 32
PpuMI rG'GwC_Cy
Cuts at: 257 1336 257
Size: 1079 5811
PshAI GACnn'nnGTC
Cuts at: 1323 1323
Size: 6890
PsiI TTA'TAA
Cuts at: 1430 1430
Size: 6890
PstI C_TGCA'G
Cuts at: 818 1073 1727 2197 2573 3751 3788 3937 4622
Size: 255 654 470 376 1178 37 149 685
Cuts at: 4622 818
Size: 3086
Fragments arranged by size:
3086 1178 685 654 470 376 255 149
37
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6890
PvuII CAG'CTG
Cuts at: 1680 2379 2466 3027 3222 3653 4315 4675 1680
Size: 699 87 561 195 431 662 360 3895
Fragments arranged by size:
3895 699 662 561 431 360 195 87
RsrII CG'GwC_CG
Cuts at: 5085 5085
Size: 6890
SacI G_AGCT'C
Cuts at: 3904 3904
Size: 6890
SalI G'TCGA_C
Cuts at: 3457 3927 3457
Size: 470 6420
SapI GCTCTTCn'nnn_
Cuts at: 931 1069 3152 3568 4913 5123 931
Size: 138 2083 416 1345 210 2698
Fragments arranged by size:
2698 2083 1345 416 210 138
SbfI CC_TGCA'GG
Cuts at: 3937 3937
Size: 6890
ScaI AGT'ACT
Cuts at: 6407 6407
Size: 6890
SexAI A'CCwGG_T
Cuts at: 1158 1158
Size: 6890
SfcI C'TryA_G
Cuts at: 33 413 814 1069 1723 2193 2569 3111 3747
Size: 380 401 255 654 470 376 542 636
Cuts at: 3747 3784 3933 4618 5611 5802 33
Size: 37 149 685 993 191 1121
Fragments arranged by size:
1121 993 685 654 636 542 470 401
380 376 255 191 149 37
SfoI GGC'GCC
Cuts at: 4322 4570 4322
Size: 248 6642
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6890
SgrAI Cr'CCGG_yG
Cuts at: 135 135
Size: 6890
SmaI CCC'GGG
Cuts at: 3912 3912
Size: 6890
SmlI C'TyrA_G
Cuts at: 729 3145 3718 4045 4960 5452 5714 729
Size: 2416 573 327 915 492 262 1905
Fragments arranged by size:
2416 1905 915 573 492 327 262
SpeI A'CTAG_T
Cuts at: 67 3075 67
Size: 3008 3882
SphI G_CATG'C
Cuts at: 3943 4443 3943
Size: 500 6390
SspI AAT'ATT
Cuts at: 772 772
Size: 6890
StuI AGG'CCT
Cuts at: 3767 3767
Size: 6890
StyI C'CwwG_G
Cuts at: 107 149 699 1301 1701 2348 4024 4378 107
Size: 42 550 602 400 647 1676 354 2619
Fragments arranged by size:
2619 1676 647 602 550 400 354 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1167 4473 4606 5065 5274 1167
Size: 3306 133 459 209 2783
Fragments arranged by size:
3306 2783 459 209 133
TatI w'GTAC_w
Cuts at: 1943 2447 6405 1943
Size: 504 3958 2428
Fragments arranged by size:
3958 2428 504
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 750 782 2041 2073 2942 2974 3133 3165 6407
Size: 32 1259 32 869 32 159 32 3242
Cuts at: 6407 6439 750
Size: 32 1201
Fragments arranged by size:
3242 1259 1201 869 159 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2689 3862 4687 2689
Size: 1173 825 4892
Fragments arranged by size:
4892 1173 825
XbaI T'CTAG_A
Cuts at: 2241 3921 2241
Size: 1680 5210
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 938 4385 938
Size: 3447 3443
XmaI C'CCGG_G
Cuts at: 3910 3910
Size: 6890
XmnI GAAnn'nnTTC
Cuts at: 649 649
Size: 6890
ZraI GAC'GTC
Cuts at: 2057 3591 2057
Size: 1534 5356
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI ApaI AscI BcgI BglI
BmtI BsiWI BsmBI CspCI EcoRV FspAI HpaI MfeI
NdeI NheI NotI NruI PacI PasI PspOMI PspXI
PsrI SacII SanDI SfiI SnaBI SrfI SwaI XhoI