Restriction map for
pF1KB7004
(Circular) (Six-base) MAPSORT of: pF1KB7004.seq Check: 2686 from: 1 to: 7133
pF1KB7004 7133 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 396 396
Size: 7133
AccI GT'mk_AC
Cuts at: 4171 4171
Size: 7133
Acc65I G'GTAC_C
Cuts at: 3237 4149 3237
Size: 912 6221
AclI AA'CG_TT
Cuts at: 6973 6973
Size: 7133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 842 1790 2494 2692 4958 5390 6137 6405 842
Size: 948 704 198 2266 432 747 268 1570
Fragments arranged by size:
2266 1570 948 747 704 432 268 198
AfeI AGC'GCT
Cuts at: 967 967
Size: 7133
AflIII A'CryG_T
Cuts at: 419 4485 5589 6566 419
Size: 4066 1104 977 986
Fragments arranged by size:
4066 1104 986 977
AgeI A'CCGG_T
Cuts at: 5521 5521
Size: 7133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2464 3441 2464
Size: 977 6156
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2521 2553 2521
Size: 32 7101
AlwNI CAG_nnn'CTG
Cuts at: 457 1791 2587 3594 3907 4438 6005 457
Size: 1334 796 1007 313 531 1567 1585
Fragments arranged by size:
1585 1567 1334 1007 796 531 313
ApaI G_GGCC'C
Cuts at: 800 1151 1939 1993 2116 2227 2303 2581 800
Size: 351 788 54 123 111 76 278 5352
Fragments arranged by size:
5352 788 351 278 123 111 76 54
ApaLI G'TGCA_C
Cuts at: 5903 5903
Size: 7133
ApoI r'AATT_y
Cuts at: 241 624 1021 1695 1973 4137 6486 6742 241
Size: 383 397 674 278 2164 2349 256 632
Fragments arranged by size:
2349 2164 674 632 397 383 278 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7133
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7133
AvaI C'yCGr_G
Cuts at: 1285 1406 1521 2523 3442 3608 4055 4153 1285
Size: 121 115 1002 919 166 447 98 4265
Fragments arranged by size:
4265 1002 919 447 166 121 115 98
AvrII C'CTAG_G
Cuts at: 1131 1131
Size: 7133
BamHI G'GATC_C
Cuts at: 2065 2720 4158 2065
Size: 655 1438 5040
Fragments arranged by size:
5040 1438 655
BanI G'GyrC_C
Cuts at: 669 1464 2178 3237 4149 4563 4811 4846 669
Size: 795 714 1059 912 414 248 35 2956
Fragments arranged by size:
2956 1059 912 795 714 414 248 35
BanII G_rGCy'C
Cuts at: 139 383 800 908 1151 1170 1939 1993 2116
Size: 244 417 108 243 19 769 54 123
Cuts at: 2116 2227 2303 2518 2581 3953 3979 4147 5177
Size: 111 76 215 63 1372 26 168 1030
Cuts at: 5177 139
Size: 2095
Fragments arranged by size:
2095 1372 1030 769 417 244 243 215
168 123 111 108 76 63 54 26
19
BbeI G_GCGC'C
Cuts at: 4567 4815 4567
Size: 248 6885
BbsI GAAGACnn'nnnn_
Cuts at: 335 403 2836 335
Size: 68 2433 4632
Fragments arranged by size:
4632 2433 68
BbvCI CC'TCA_GC
Cuts at: 3079 3079
Size: 7133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1387 1421 90
Size: 34 1263 34 5802
Fragments arranged by size:
5802 1263 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5031 5792 7133 5031
Size: 761 1341 5031
Fragments arranged by size:
5031 1341 761
BclI T'GATC_A
Cuts at: 4653 4653
Size: 7133
BfrBI ATG'CAT
Cuts at: 111 111
Size: 7133
BglI GCCn_nnn'nGGC
Cuts at: 1114 2144 3380 3948 1114
Size: 1030 1236 568 4299
Fragments arranged by size:
4299 1236 1030 568
BglII A'GATC_T
Cuts at: 3504 4648 6685 3504
Size: 1144 2037 3952
Fragments arranged by size:
3952 2037 1144
BlpI GC'TnA_GC
Cuts at: 2165 4245 2165
Size: 2080 5053
Bme1580I G_kGCm'C
Cuts at: 800 1151 1467 1939 1993 2116 2227 2303 2581
Size: 351 316 472 54 123 111 76 278
Cuts at: 2581 4758 4851 5907 800
Size: 2177 93 1056 2026
Fragments arranged by size:
2177 2026 1056 472 351 316 278 123
111 93 76 54
BmgBI CAC'GTC
Cuts at: 700 1161 4074 700
Size: 461 2913 3759
Fragments arranged by size:
3759 2913 461
BmrI ACTGGGnnnn_n'
Cuts at: 803 925 1236 2936 3317 4345 4426 4761 6905
Size: 122 311 1700 381 1028 81 335 2144
Cuts at: 6905 803
Size: 1031
Fragments arranged by size:
2144 1700 1031 1028 381 335 311 122
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 155 5500 155
Size: 5345 1788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 973 1193 1360 1670 2375 2858 2990 3260
Size: 587 220 167 310 705 483 132 270
Cuts at: 3260 3455 3548 3965 386
Size: 195 93 417 3554
Fragments arranged by size:
3554 705 587 483 417 310 270 220
195 167 132 93
Bpu10I CC'TnA_GC
Cuts at: 3079 3079
Size: 7133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1166 2026 2518 2632 3227 4309 5188 5680 5978
Size: 860 492 114 595 1082 879 492 298
Cuts at: 5978 1166
Size: 2321
Fragments arranged by size:
2321 1082 879 860 595 492 492 298
114
BsaI GGTCTCn'nnnn_
Cuts at: 1816 3792 6837 1816
Size: 1976 3045 2112
Fragments arranged by size:
3045 2112 1976
BsaAI yAC'GTr
Cuts at: 420 4486 420
Size: 4066 3067
BsaBI GATnn'nnATC
Cuts at: 6 2045 4647 6684 6
Size: 2039 2602 2037 455
Fragments arranged by size:
2602 2039 2037 455
BsaHI Gr'CG_yC
Cuts at: 4564 4812 5514 7010 4564
Size: 248 702 1496 4687
Fragments arranged by size:
4687 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3535 3747 4322 4843 5521 5795 5942 6353 3535
Size: 212 575 521 678 274 147 411 4315
Fragments arranged by size:
4315 678 575 521 411 274 212 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2373 2403 2676 2706 2856 2886 2373
Size: 30 273 30 150 30 6620
Fragments arranged by size:
6620 273 150 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 147 1350 2134 2378 3149 147
Size: 1203 784 244 771 4131
Fragments arranged by size:
4131 1203 784 771 244
BseYI C'CCAG_C
Cuts at: 227 293 600 1460 1806 1846 1880 2104 2204
Size: 66 307 860 346 40 34 224 100
Cuts at: 2204 2511 2580 2760 2769 3098 3388 3583 4558
Size: 307 69 180 9 329 290 195 975
Cuts at: 4558 5316 5893 6389 227
Size: 758 577 496 971
Fragments arranged by size:
975 971 860 758 577 496 346 329
307 307 290 224 195 180 100 69
66 40 34 9
BsiEI CG_ry'CG
Cuts at: 104 4675 4721 5929 104
Size: 4571 46 1208 1308
Fragments arranged by size:
4571 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 139 959 1035 1170 2201 4147 4925 5115 5907
Size: 820 76 135 1031 1946 778 190 792
Cuts at: 5907 139
Size: 1365
Fragments arranged by size:
1946 1365 1031 820 792 778 190 135
76
BsmI GAATG_Cn'
Cuts at: 111 2451 111
Size: 2340 4793
BsmBI CGTCTCn'nnnn_
Cuts at: 492 492
Size: 7133
Bsp1286I G_dGCh'C
Cuts at: 139 383 800 908 959 1035 1151 1170 1467
Size: 244 417 108 51 76 116 19 297
Cuts at: 1467 1939 1993 2116 2201 2227 2303 2518 2581
Size: 472 54 123 85 26 76 215 63
Cuts at: 2581 3953 3979 4147 4758 4851 4925 5115 5177
Size: 1372 26 168 611 93 74 190 62
Cuts at: 5177 5907 139
Size: 730 1365
Fragments arranged by size:
1372 1365 730 611 472 417 297 244
215 190 168 123 116 108 93 85
76 76 74 63 62 54 51 26
26 19
BspEI T'CCGG_A
Cuts at: 3747 6353 3747
Size: 2606 4527
BspHI T'CATG_A
Cuts at: 4031 6309 4031
Size: 2278 4855
BspMI ACCTGCnnnn'nnnn_
Cuts at: 396 3288 3404 4183 4397 4699 5080 396
Size: 2892 116 779 214 302 381 2449
Fragments arranged by size:
2892 2449 779 381 302 214 116
BsrBI CCG'CTC
Cuts at: 50 5425 5479 6963 7128 50
Size: 5375 54 1484 165 55
Fragments arranged by size:
5375 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2942 5045 2942
Size: 2103 5030
BsrFI r'CCGG_y
Cuts at: 2321 5131 5312 5521 2321
Size: 2810 181 209 3933
Fragments arranged by size:
3933 2810 209 181
BssSI C'ACGA_G
Cuts at: 5404 5762 5404
Size: 358 6775
BstAPI GCAn_nnn'nTGC
Cuts at: 4510 4510
Size: 7133
BstBI TT'CG_AA
Cuts at: 5494 5494
Size: 7133
BstXI CCAn_nnnn'nTGG
Cuts at: 3646 4102 3646
Size: 456 6677
BstYI r'GATC_y
Cuts at: 1 864 2065 2720 3504 4158 4625 4648 4983
Size: 863 1201 655 784 654 467 23 335
Cuts at: 4983 5229 6241 6327 6339 6685 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1201 1012 863 784 655 654 467 449
346 335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2117 2117
Size: 7133
BtgI C'CryG_G
Cuts at: 357 1829 2750 3059 3140 3911 357
Size: 1472 921 309 81 771 3579
Fragments arranged by size:
3579 1472 921 771 309 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2537 5083 5264 5427 2537
Size: 2546 181 163 4243
Fragments arranged by size:
4243 2546 181 163
BtsI GCAGTG_nn'
Cuts at: 775 830 1372 2182 4376 775
Size: 55 542 810 2194 3532
Fragments arranged by size:
3532 2194 810 542 55
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3041 3076 3122 3157 3041
Size: 35 46 35 7017
Fragments arranged by size:
7017 46 35 35
DraI TTT'AAA
Cuts at: 4132 4132
Size: 7133
DraIII CAC_nnn'GTG
Cuts at: 1039 1039
Size: 7133
DrdI GACnn_nn'nnGTC
Cuts at: 1387 4839 5697 1387
Size: 3452 858 2823
Fragments arranged by size:
3452 2823 858
EaeI y'GGCC_r
Cuts at: 360 437 608 1660 1707 1731 1826 2981 3381
Size: 77 171 1052 47 24 95 1155 400
Cuts at: 3381 3876 4718 4892 5283 5310 6655 360
Size: 495 842 174 391 27 1345 838
Fragments arranged by size:
1345 1155 1052 842 838 495 400 391
174 171 95 77 47 27 24
EagI C'GGCC_G
Cuts at: 4718 4718
Size: 7133
EarI CTCTTCn'nnn_
Cuts at: 861 1322 1327 1766 2694 5156 5366 861
Size: 461 5 439 928 2462 210 2628
Fragments arranged by size:
2628 2462 928 461 439 210 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 869 2427 5341 5651 5797 6943 869
Size: 1558 2914 310 146 1146 1059
Fragments arranged by size:
2914 1558 1146 1059 310 146
EcoICRI GAG'CTC
Cuts at: 137 1168 4145 137
Size: 1031 2977 3125
Fragments arranged by size:
3125 2977 1031
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 842 973 1193 1360 1670 1790 2375 2494
Size: 456 131 220 167 310 120 585 119
Cuts at: 2494 2692 2858 2990 3260 3455 3548 3965 4958
Size: 198 166 132 270 195 93 417 993
Cuts at: 4958 5390 6137 6405 386
Size: 432 747 268 1114
Fragments arranged by size:
1114 993 747 585 456 432 417 310
270 268 220 198 195 167 166 132
131 120 119 93
EcoNI CCTnn'n_nnAGG
Cuts at: 749 940 1135 1454 1733 749
Size: 191 195 319 279 6149
Fragments arranged by size:
6149 319 279 195 191
EcoO109I rG'GnC_Cy
Cuts at: 1141 1148 1258 1423 1935 1936 1990 2113 2224
Size: 7 110 165 512 1 54 123 111
Cuts at: 2224 3145 3168 3178 3218 3373 3439 3448 3643
Size: 921 23 10 40 155 66 9 195
Cuts at: 3643 3832 3940 4015 4052 4099 4272 1141
Size: 189 108 75 37 47 173 4002
Fragments arranged by size:
4002 921 512 195 189 173 165 155
123 111 110 108 75 66 54 47
40 37 23 10 9 7 1
EcoRI G'AATT_C
Cuts at: 624 1021 1973 4137 624
Size: 397 952 2164 3620
Fragments arranged by size:
3620 2164 952 397
EcoRV GAT'ATC
Cuts at: 557 990 557
Size: 433 6700
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3394 5152 3394
Size: 1758 5375
FspI TGC'GCA
Cuts at: 4914 4914
Size: 7133
HaeII r_GCGC'y
Cuts at: 541 969 1887 4567 4815 5837 6816 541
Size: 428 918 2680 248 1022 979 858
Fragments arranged by size:
2680 1022 979 918 858 428 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 177 209 922 954 1377 1409 2070 2102 2676
Size: 32 713 32 423 32 661 32 574
Cuts at: 2676 2708 2983 3015 3114 3146 3225 3257 3819
Size: 32 275 32 99 32 79 32 562
Cuts at: 3819 3851 3882 3914 4975 5005 5007 5037 177
Size: 32 31 32 1061 30 2 30 2273
Fragments arranged by size:
2273 1061 713 661 574 562 423 275
99 79 32 32 32 32 32 32
32 32 32 32 31 30 30 2
HincII GTy'rAC
Cuts at: 2058 4172 2058
Size: 2114 5019
KasI G'GCGC_C
Cuts at: 4563 4811 4563
Size: 248 6885
KpnI G_GTAC'C
Cuts at: 3241 4153 3241
Size: 912 6221
MfeI C'AATT_G
Cuts at: 2294 2294
Size: 7133
MluI A'CGCG_T
Cuts at: 6566 6566
Size: 7133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2093 3546 3840 5804 5988 2093
Size: 1453 294 1964 184 3238
Fragments arranged by size:
3238 1964 1453 294 184
MscI TGG'CCA
Cuts at: 362 439 1662 1709 1828 2983 4894 362
Size: 77 1223 47 119 1155 1911 2601
Fragments arranged by size:
2601 1911 1223 1155 119 77 47
MslI CAynn'nnrTG
Cuts at: 1193 1855 2679 3889 3991 5122 5249 1193
Size: 662 824 1210 102 1131 127 3077
Fragments arranged by size:
3077 1210 1131 824 662 127 102
MspA1I CmG'CkG
Cuts at: 231 951 1724 1791 1806 2559 2584 3904 4244
Size: 720 773 67 15 753 25 1320 340
Cuts at: 4244 4558 4918 5931 6176 231
Size: 314 360 1013 245 1188
Fragments arranged by size:
1320 1188 1013 773 753 720 360 340
314 245 67 25 15
NaeI GCC'GGC
Cuts at: 2323 5314 2323
Size: 2991 4142
NarI GG'CG_CC
Cuts at: 4564 4812 4564
Size: 248 6885
NcoI C'CATG_G
Cuts at: 357 1829 2750 357
Size: 1472 921 4740
Fragments arranged by size:
4740 1472 921
NdeI CA'TA_TG
Cuts at: 1277 2333 1277
Size: 1056 6077
NgoMIV G'CCGG_C
Cuts at: 2321 5312 2321
Size: 2991 4142
NsiI A_TGCA'T
Cuts at: 113 113
Size: 7133
NspI r_CATG'y
Cuts at: 1002 1506 1551 2157 2571 3483 3669 4186 4686
Size: 504 45 606 414 912 186 517 500
Cuts at: 4686 5593 1002
Size: 907 2542
Fragments arranged by size:
2542 912 907 606 517 504 500 414
186 45
PasI CC'CwG_GG
Cuts at: 840 1819 3264 3639 3964 840
Size: 979 1445 375 325 4009
Fragments arranged by size:
4009 1445 979 375 325
PciI A'CATG_T
Cuts at: 5589 5589
Size: 7133
PfoI T'CCnGG_A
Cuts at: 1376 3645 1376
Size: 2269 4864
PmeI GTTT'AAAC
Cuts at: 4132 4132
Size: 7133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2887 2919 3602 3634 6272 6304 2887
Size: 32 683 32 2638 32 3716
Fragments arranged by size:
3716 2638 683 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1141 1258 1423 3145 3168 3218 3448 3643 3832
Size: 117 165 1722 23 50 230 195 189
Cuts at: 3832 4015 4099 1141
Size: 183 84 4175
Fragments arranged by size:
4175 1722 230 195 189 183 165 117
84 50 23
PshAI GACnn'nnGTC
Cuts at: 832 832
Size: 7133
PspOMI G'GGCC_C
Cuts at: 796 1147 1935 1989 2112 2223 2299 2577 796
Size: 351 788 54 123 111 76 278 5352
Fragments arranged by size:
5352 788 351 278 123 111 76 54
PspXI vC'TCGA_Gb
Cuts at: 3608 3608
Size: 7133
PstI C_TGCA'G
Cuts at: 453 650 1313 3512 4180 4865 453
Size: 197 663 2199 668 685 2721
Fragments arranged by size:
2721 2199 685 668 663 197
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7133
PvuII CAG'CTG
Cuts at: 231 951 1791 1806 2559 2584 3904 4558 4918
Size: 720 840 15 753 25 1320 654 360
Cuts at: 4918 231
Size: 2446
Fragments arranged by size:
2446 1320 840 753 720 654 360 25
15
RsrII CG'GwC_CG
Cuts at: 5328 5328
Size: 7133
SacI G_AGCT'C
Cuts at: 139 1170 4147 139
Size: 1031 2977 3125
Fragments arranged by size:
3125 2977 1031
SalI G'TCGA_C
Cuts at: 4170 4170
Size: 7133
SanDI GG'GwC_CC
Cuts at: 3145 3168 3218 4015 3145
Size: 23 50 797 6263
Fragments arranged by size:
6263 797 50 23
SapI GCTCTTCn'nnn_
Cuts at: 861 5156 5366 861
Size: 4295 210 2628
Fragments arranged by size:
4295 2628 210
SbfI CC_TGCA'GG
Cuts at: 4180 4180
Size: 7133
ScaI AGT'ACT
Cuts at: 6650 6650
Size: 7133
SexAI A'CCwGG_T
Cuts at: 1638 1638
Size: 7133
SfcI C'TryA_G
Cuts at: 33 200 329 449 646 749 1309 2804 3508
Size: 167 129 120 197 103 560 1495 704
Cuts at: 3508 4176 4861 5854 6045 33
Size: 668 685 993 191 1121
Fragments arranged by size:
1495 1121 993 704 685 668 560 197
191 167 129 120 103
SfiI GGCCn_nnn'nGGCC
Cuts at: 1114 3380 1114
Size: 2266 4867
SfoI GGC'GCC
Cuts at: 4565 4813 4565
Size: 248 6885
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7133
SmaI CCC'GGG
Cuts at: 3444 4057 4155 3444
Size: 613 98 6422
Fragments arranged by size:
6422 613 98
SmlI C'TyrA_G
Cuts at: 1181 2005 2533 2647 3242 3608 4288 5203 5695
Size: 824 528 114 595 366 680 915 492
Cuts at: 5695 5957 1181
Size: 262 2357
Fragments arranged by size:
2357 915 824 680 595 528 492 366
262 114
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7133
SphI G_CATG'C
Cuts at: 1002 2571 3483 4186 4686 1002
Size: 1569 912 703 500 3449
Fragments arranged by size:
3449 1569 912 703 500
SspI AAT'ATT
Cuts at: 3601 3601
Size: 7133
StyI C'CwwG_G
Cuts at: 357 1131 1758 1829 2420 2750 3378 4095 4267
Size: 774 627 71 591 330 628 717 172
Cuts at: 4267 4621 357
Size: 354 2869
Fragments arranged by size:
2869 774 717 628 627 591 354 330
172 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 675 693 3973 4072 4716 4849 5308 5517 675
Size: 18 3280 99 644 133 459 209 2291
Fragments arranged by size:
3280 2291 644 459 209 133 99 18
TatI w'GTAC_w
Cuts at: 2854 3279 6648 2854
Size: 425 3369 3339
Fragments arranged by size:
3369 3339 425
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 265 297 2373 2405 6650 6682 265
Size: 32 2076 32 4245 32 716
Fragments arranged by size:
4245 2076 716 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1640 4930 1640
Size: 3290 3843
XbaI T'CTAG_A
Cuts at: 1633 4164 1633
Size: 2531 4602
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1894 2212 4628 1894
Size: 318 2416 4399
Fragments arranged by size:
4399 2416 318
XhoI C'TCGA_G
Cuts at: 3608 3608
Size: 7133
XmaI C'CCGG_G
Cuts at: 3442 4055 4153 3442
Size: 613 98 6422
Fragments arranged by size:
6422 613 98
XmnI GAAnn'nnTTC
Cuts at: 1021 1220 1021
Size: 199 6934
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PasI PciI PfoI PmeI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AscI BaeI BmtI BsgI
BsiWI BsrGI BssHII BstEII BstZ17I ClaI FseI FspAI
HindIII HpaI NheI NotI NruI PacI PflMI PmlI
PsiI PsrI SacII SgrAI SnaBI SrfI StuI SwaI
ZraI