Restriction map for
pF1KB7003
(Circular) (Six-base) MAPSORT of: pF1KB7003.seq Check: 6260 from: 1 to: 6974
pF1KB7003 6974 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1641 1907 2506 1641
Size: 266 599 6109
Fragments arranged by size:
6109 599 266
AccI GT'mk_AC
Cuts at: 2986 4012 2986
Size: 1026 5948
Acc65I G'GTAC_C
Cuts at: 171 3990 171
Size: 3819 3155
AclI AA'CG_TT
Cuts at: 1910 3032 6814 1910
Size: 1122 3782 2070
Fragments arranged by size:
3782 2070 1122
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 528 1852 2030 2299 4799 5231 5978 6246 528
Size: 1324 178 269 2500 432 747 268 1256
Fragments arranged by size:
2500 1324 1256 747 432 269 268 178
AfeI AGC'GCT
Cuts at: 1176 1176
Size: 6974
AflII C'TTAA_G
Cuts at: 3103 3264 3103
Size: 161 6813
AflIII A'CryG_T
Cuts at: 3449 4326 5430 6407 3449
Size: 877 1104 977 4016
Fragments arranged by size:
4016 1104 977 877
AgeI A'CCGG_T
Cuts at: 5362 5362
Size: 6974
AleI CACnn'nnGTG
Cuts at: 715 2042 3148 3182 715
Size: 1327 1106 34 4507
Fragments arranged by size:
4507 1327 1106 34
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3565 3597 3565
Size: 32 6942
AlwNI CAG_nnn'CTG
Cuts at: 994 1381 1732 2352 4279 5846 994
Size: 387 351 620 1927 1567 2122
Fragments arranged by size:
2122 1927 1567 620 387 351
ApaLI G'TGCA_C
Cuts at: 200 5744 200
Size: 5544 1430
ApoI r'AATT_y
Cuts at: 330 661 890 999 1224 1959 2564 2583 2695
Size: 331 229 109 225 735 605 19 112
Cuts at: 2695 2915 3912 3978 6327 6583 330
Size: 220 997 66 2349 256 721
Fragments arranged by size:
2349 997 735 721 605 331 256 229
225 220 112 109 66 19
AseI AT'TA_AT
Cuts at: 20 2600 3401 3641 20
Size: 2580 801 240 3353
Fragments arranged by size:
3353 2580 801 240
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6974
AvaI C'yCGr_G
Cuts at: 3994 3994
Size: 6974
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3671 3704 3671
Size: 33 6941
BamHI G'GATC_C
Cuts at: 1987 3999 1987
Size: 2012 4962
BanI G'GyrC_C
Cuts at: 171 2827 3922 3990 4404 4652 4687 171
Size: 2656 1095 68 414 248 35 2458
Fragments arranged by size:
2656 2458 1095 414 248 68 35
BanII G_rGCy'C
Cuts at: 3988 5018 3988
Size: 1030 5944
BbeI G_GCGC'C
Cuts at: 4408 4656 4408
Size: 248 6726
BbsI GAAGACnn'nnnn_
Cuts at: 292 1235 1787 1860 2393 2412 2432 2611 292
Size: 943 552 73 533 19 20 179 4655
Fragments arranged by size:
4655 943 552 533 179 73 20 19
BciVI GTATCCnnnnn_n'
Cuts at: 1769 2727 4872 5633 6974 1769
Size: 958 2145 761 1341 1769
Fragments arranged by size:
2145 1769 1341 958 761
BclI T'GATC_A
Cuts at: 1281 4494 1281
Size: 3213 3761
BfrBI ATG'CAT
Cuts at: 2071 2272 3197 2071
Size: 201 925 5848
Fragments arranged by size:
5848 925 201
BglII A'GATC_T
Cuts at: 4489 6526 4489
Size: 2037 4937
BlpI GC'TnA_GC
Cuts at: 314 4086 314
Size: 3772 3202
Bme1580I G_kGCm'C
Cuts at: 204 4599 4692 5748 204
Size: 4395 93 1056 1430
Fragments arranged by size:
4395 1430 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2654 4186 4267 4602 6746 2654
Size: 1532 81 335 2144 2882
Fragments arranged by size:
2882 2144 1532 335 81
BmtI G_CTAG'C
Cuts at: 2498 2498
Size: 6974
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2553 5341 2553
Size: 2788 4186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 1568 2249 245
Size: 1323 681 4970
Fragments arranged by size:
4970 1323 681
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 767 780 1581 2121 4150 5029 5521 5819
Size: 285 13 801 540 2029 879 492 298
Cuts at: 5819 482
Size: 1637
Fragments arranged by size:
2029 1637 879 801 540 492 298 285
13
BsaI GGTCTCn'nnnn_
Cuts at: 2097 6678 2097
Size: 4581 2393
BsaAI yAC'GTr
Cuts at: 4327 4327
Size: 6974
BsaBI GATnn'nnATC
Cuts at: 6 2273 4488 6525 6
Size: 2267 2215 2037 455
Fragments arranged by size:
2267 2215 2037 455
BsaHI Gr'CG_yC
Cuts at: 4405 4653 5355 6851 4405
Size: 248 702 1496 4528
Fragments arranged by size:
4528 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4163 4684 5362 5636 5783 6194 4163
Size: 521 678 274 147 411 4943
Fragments arranged by size:
4943 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1508 4399 5157 5734 6230 1508
Size: 2891 758 577 496 2252
Fragments arranged by size:
2891 2252 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3361 4516 4562 5770 104
Size: 3257 1155 46 1208 1308
Fragments arranged by size:
3257 1308 1208 1155 46
BsiHKAI G_wGCw'C
Cuts at: 204 2488 3988 4766 4956 5748 204
Size: 2284 1500 778 190 792 1430
Fragments arranged by size:
2284 1500 1430 792 778 190
BsmI GAATG_Cn'
Cuts at: 436 1057 2025 2071 2514 3019 436
Size: 621 968 46 443 505 4391
Fragments arranged by size:
4391 968 621 505 443 46
BsmBI CGTCTCn'nnnn_
Cuts at: 3949 3949
Size: 6974
Bsp1286I G_dGCh'C
Cuts at: 204 2488 3988 4599 4692 4766 4956 5018 5748
Size: 2284 1500 611 93 74 190 62 730
Cuts at: 5748 204
Size: 1430
Fragments arranged by size:
2284 1500 1430 730 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 6194 6194
Size: 6974
BspHI T'CATG_A
Cuts at: 1701 3009 6150 1701
Size: 1308 3141 2525
Fragments arranged by size:
3141 2525 1308
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1641 1907 2506 4024 4238 4540 4921 1641
Size: 266 599 1518 214 302 381 3694
Fragments arranged by size:
3694 1518 599 381 302 266 214
BsrBI CCG'CTC
Cuts at: 50 3260 5266 5320 6804 6969 50
Size: 3210 2006 54 1484 165 55
Fragments arranged by size:
3210 2006 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1498 2474 2678 4886 1498
Size: 976 204 2208 3586
Fragments arranged by size:
3586 2208 976 204
BsrFI r'CCGG_y
Cuts at: 4972 5153 5362 4972
Size: 181 209 6584
Fragments arranged by size:
6584 209 181
BsrGI T'GTAC_A
Cuts at: 1743 1743
Size: 6974
BssSI C'ACGA_G
Cuts at: 5245 5603 5245
Size: 358 6616
BstAPI GCAn_nnn'nTGC
Cuts at: 2213 2877 2886 4351 2213
Size: 664 9 1465 4836
Fragments arranged by size:
4836 1465 664 9
BstBI TT'CG_AA
Cuts at: 3798 5335 3798
Size: 1537 5437
BstXI CCAn_nnnn'nTGG
Cuts at: 3189 3279 3189
Size: 90 6884
BstYI r'GATC_y
Cuts at: 1 1364 1987 3999 4466 4489 4824 5070 6082
Size: 1363 623 2012 467 23 335 246 1012
Cuts at: 6082 6168 6180 6526 1
Size: 86 12 346 449
Fragments arranged by size:
2012 1363 1012 623 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 926 2108 3577 926
Size: 1182 1469 4323
Fragments arranged by size:
4323 1469 1182
BtgI C'CryG_G
Cuts at: 107 818 107
Size: 711 6263
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2138 4924 5105 5268 2138
Size: 2786 181 163 3844
Fragments arranged by size:
3844 2786 181 163
BtsI GCAGTG_nn'
Cuts at: 1611 1885 3218 3507 3794 4217 1611
Size: 274 1333 289 287 423 4368
Fragments arranged by size:
4368 1333 423 289 287 274
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 254 289 3053 3088 254
Size: 35 2764 35 4140
Fragments arranged by size:
4140 2764 35 35
DraI TTT'AAA
Cuts at: 557 705 3323 3973 557
Size: 148 2618 650 3558
Fragments arranged by size:
3558 2618 650 148
DraIII CAC_nnn'GTG
Cuts at: 2213 2213
Size: 6974
DrdI GACnn_nn'nnGTC
Cuts at: 4680 5538 4680
Size: 858 6116
EaeI y'GGCC_r
Cuts at: 2775 4559 4733 5124 5151 6496 2775
Size: 1784 174 391 27 1345 3253
Fragments arranged by size:
3253 1784 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4559 4559
Size: 6974
EarI CTCTTCn'nnn_
Cuts at: 464 1748 2663 2730 3472 3515 4997 5207 464
Size: 1284 915 67 742 43 1482 210 2231
Fragments arranged by size:
2231 1482 1284 915 742 210 67 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5182 5492 5638 6784 5182
Size: 310 146 1146 5372
Fragments arranged by size:
5372 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3986 3986
Size: 6974
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 528 1568 1852 2030 2249 2299 4799 5231
Size: 283 1040 284 178 219 50 2500 432
Cuts at: 5231 5978 6246 245
Size: 747 268 973
Fragments arranged by size:
2500 1040 973 747 432 284 283 268
219 178 50
EcoO109I rG'GnC_Cy
Cuts at: 4113 4113
Size: 6974
EcoRI G'AATT_C
Cuts at: 3912 3978 3912
Size: 66 6908
EcoRV GAT'ATC
Cuts at: 811 811
Size: 6974
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2189 4993 2189
Size: 2804 4170
FspI TGC'GCA
Cuts at: 4755 4755
Size: 6974
HaeII r_GCGC'y
Cuts at: 1178 4408 4656 5678 6657 1178
Size: 3230 248 1022 979 1495
Fragments arranged by size:
3230 1495 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 218 250 384 416 614 646 753 785 1273
Size: 32 134 32 198 32 107 32 488
Cuts at: 1273 1305 1863 1895 1990 2022 2968 3000 3473
Size: 32 558 32 95 32 946 32 473
Cuts at: 3473 3505 4816 4846 4848 4878 218
Size: 32 1311 30 2 30 2314
Fragments arranged by size:
2314 1311 946 558 488 473 198 134
107 95 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3740 4013 3740
Size: 273 6701
HindIII A'AGCT_T
Cuts at: 268 268
Size: 6974
KasI G'GCGC_C
Cuts at: 4404 4652 4404
Size: 248 6726
KpnI G_GTAC'C
Cuts at: 175 3994 175
Size: 3819 3155
MluI A'CGCG_T
Cuts at: 6407 6407
Size: 6974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 318 763 830 1379 1392 1965 2318 2744 5645
Size: 445 67 549 13 573 353 426 2901
Cuts at: 5645 5829 318
Size: 184 1463
Fragments arranged by size:
2901 1463 573 549 445 426 353 184
67 13
MscI TGG'CCA
Cuts at: 2777 4735 2777
Size: 1958 5016
MslI CAynn'nnrTG
Cuts at: 195 715 2042 2479 2683 3148 3182 4963 5090
Size: 520 1327 437 204 465 34 1781 127
Cuts at: 5090 195
Size: 2079
Fragments arranged by size:
2079 1781 1327 520 465 437 204 127
34
MspA1I CmG'CkG
Cuts at: 2781 3336 4085 4399 4759 5772 6017 2781
Size: 555 749 314 360 1013 245 3738
Fragments arranged by size:
3738 1013 749 555 360 314 245
NaeI GCC'GGC
Cuts at: 5155 5155
Size: 6974
NarI GG'CG_CC
Cuts at: 4405 4653 4405
Size: 248 6726
NcoI C'CATG_G
Cuts at: 107 818 107
Size: 711 6263
NdeI CA'TA_TG
Cuts at: 2180 2180
Size: 6974
NgoMIV G'CCGG_C
Cuts at: 5153 5153
Size: 6974
NheI G'CTAG_C
Cuts at: 2494 2494
Size: 6974
NsiI A_TGCA'T
Cuts at: 2073 2274 3199 2073
Size: 201 925 5848
Fragments arranged by size:
5848 925 201
NspI r_CATG'y
Cuts at: 2220 2802 2875 3197 3237 3453 4027 4527 5434
Size: 582 73 322 40 216 574 500 907
Cuts at: 5434 2220
Size: 3760
Fragments arranged by size:
3760 907 582 574 500 322 216 73
40
PciI A'CATG_T
Cuts at: 3449 5430 3449
Size: 1981 4993
PmeI GTTT'AAAC
Cuts at: 3973 3973
Size: 6974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6113 6145 6113
Size: 32 6942
PsiI TTA'TAA
Cuts at: 800 800
Size: 6974
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1856 1888 2738 2770 1856
Size: 32 850 32 6060
Fragments arranged by size:
6060 850 32 32
PstI C_TGCA'G
Cuts at: 3789 4021 4706 3789
Size: 232 685 6057
Fragments arranged by size:
6057 685 232
PvuI CG_AT'CG
Cuts at: 104 3361 104
Size: 3257 3717
PvuII CAG'CTG
Cuts at: 2781 3336 4399 4759 2781
Size: 555 1063 360 4996
Fragments arranged by size:
4996 1063 555 360
RsrII CG'GwC_CG
Cuts at: 5169 5169
Size: 6974
SacI G_AGCT'C
Cuts at: 3988 3988
Size: 6974
SalI G'TCGA_C
Cuts at: 4011 4011
Size: 6974
SapI GCTCTTCn'nnn_
Cuts at: 4997 5207 4997
Size: 210 6764
SbfI CC_TGCA'GG
Cuts at: 4021 4021
Size: 6974
ScaI AGT'ACT
Cuts at: 6491 6491
Size: 6974
SexAI A'CCwGG_T
Cuts at: 148 148
Size: 6974
SfcI C'TryA_G
Cuts at: 33 255 565 918 2093 3305 3785 4017 4702
Size: 222 310 353 1175 1212 480 232 685
Cuts at: 4702 5695 5886 33
Size: 993 191 1121
Fragments arranged by size:
1212 1175 1121 993 685 480 353 310
232 222 191
SfoI GGC'GCC
Cuts at: 4406 4654 4406
Size: 248 6726
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6974
SmaI CCC'GGG
Cuts at: 3996 3996
Size: 6974
SmlI C'TyrA_G
Cuts at: 497 759 782 1560 2100 3103 3264 4129 5044
Size: 262 23 778 540 1003 161 865 915
Cuts at: 5044 5536 5798 497
Size: 492 262 1673
Fragments arranged by size:
1673 1003 915 865 778 540 492 262
262 161 23
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6974
SphI G_CATG'C
Cuts at: 2220 3197 4027 4527 2220
Size: 977 830 500 4667
Fragments arranged by size:
4667 977 830 500
SspI AAT'ATT
Cuts at: 777 2145 3319 3559 3699 777
Size: 1368 1174 240 140 4052
Fragments arranged by size:
4052 1368 1174 240 140
StyI C'CwwG_G
Cuts at: 107 593 818 863 2966 3120 4108 4462 107
Size: 486 225 45 2103 154 988 354 2619
Fragments arranged by size:
2619 2103 988 486 354 225 154 45
SwaI ATTT'AAAT
Cuts at: 3323 3323
Size: 6974
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2054 2241 4557 4690 5149 5358 2054
Size: 187 2316 133 459 209 3670
Fragments arranged by size:
3670 2316 459 209 187 133
TatI w'GTAC_w
Cuts at: 1743 2060 6489 1743
Size: 317 4429 2228
Fragments arranged by size:
4429 2228 317
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2604 2636 3810 3842 6491 6523 2604
Size: 32 1174 32 2649 32 3055
Fragments arranged by size:
3055 2649 1174 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2235 4771 2235
Size: 2536 4438
XbaI T'CTAG_A
Cuts at: 4005 4005
Size: 6974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 645 4469 645
Size: 3824 3150
XmaI C'CCGG_G
Cuts at: 3994 3994
Size: 6974
XmnI GAAnn'nnTTC
Cuts at: 2015 2015
Size: 6974
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NsiI NspI PciI
PmeI PpiI PsiI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AhdI AlfI ApaI AscI AvrII BbvCI BcgI
BglI BmgBI Bpu10I BsaXI BseRI BsgI BsiWI BssHII
BstEII BstZ17I ClaI EcoNI FseI FspAI HpaI MfeI
NotI NruI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PspOMI PspXI SacII SanDI SfiI SgrAI SnaBI
SrfI StuI XhoI ZraI