Restriction map for
pF1KB7002
(Circular) (Six-base) MAPSORT of: pF1KB7002.seq Check: 360 from: 1 to: 12194
pF1KB7002 12194 bp
With 182 enzymes: SgfI *
December 28, 2009 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 8736 8736
Size: 12194
AatII G_ACGT'C
Cuts at: 5586 5586
Size: 12194
AccI GT'mk_AC
Cuts at: 296 4816 8138 9232 296
Size: 4520 3322 1094 3258
Fragments arranged by size:
4520 3322 3258 1094
Acc65I G'GTAC_C
Cuts at: 1491 9210 1491
Size: 7719 4475
AclI AA'CG_TT
Cuts at: 7281 12034 7281
Size: 4753 7441
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 560 3193 3998 5897 6301 6368 6428 8945 10019
Size: 2633 805 1899 404 67 60 2517 1074
Cuts at: 10019 10451 11198 11466 560
Size: 432 747 268 1288
Fragments arranged by size:
2633 2517 1899 1288 1074 805 747 432
404 268 67 60
AfeI AGC'GCT
Cuts at: 187 187
Size: 12194
AflIII A'CryG_T
Cuts at: 2756 3476 4871 7200 9546 10650 11627 2756
Size: 720 1395 2329 2346 1104 977 3323
Fragments arranged by size:
3323 2346 2329 1395 1104 977 720
AgeI A'CCGG_T
Cuts at: 10582 10582
Size: 12194
AleI CACnn'nnGTG
Cuts at: 7081 7081
Size: 12194
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 859 1178 8772 859
Size: 319 7594 4281
Fragments arranged by size:
7594 4281 319
AlwNI CAG_nnn'CTG
Cuts at: 126 1941 5889 6346 7400 7801 8578 8947 9076
Size: 1815 3948 457 1054 401 777 369 129
Cuts at: 9076 9499 11066 126
Size: 423 1567 1254
Fragments arranged by size:
3948 1815 1567 1254 1054 777 457 423
401 369 129
ApaLI G'TGCA_C
Cuts at: 3260 7196 10964 3260
Size: 3936 3768 4490
Fragments arranged by size:
4490 3936 3768
ApoI r'AATT_y
Cuts at: 387 549 631 649 1694 2477 2763 3694 4732
Size: 162 82 18 1045 783 286 931 1038
Cuts at: 4732 5308 6193 6385 6450 7020 7554 7916 8120
Size: 576 885 192 65 570 534 362 204
Cuts at: 8120 9178 9198 11547 11803 387
Size: 1058 20 2349 256 778
Fragments arranged by size:
2349 1058 1045 1038 931 885 783 778
576 570 534 362 286 256 204 192
162 82 65 20 18
AseI AT'TA_AT
Cuts at: 20 977 2291 5476 5657 8339 20
Size: 957 1314 3185 181 2682 3875
Fragments arranged by size:
3875 3185 2682 1314 957 181
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 12194
AvaI C'yCGr_G
Cuts at: 800 9214 800
Size: 8414 3780
AvrII C'CTAG_G
Cuts at: 847 847
Size: 12194
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2878 2911 4063 4096 8705 8738 2878
Size: 33 1152 33 4609 33 6334
Fragments arranged by size:
6334 4609 1152 33 33 33
BamHI G'GATC_C
Cuts at: 462 1122 2196 6529 9219 462
Size: 660 1074 4333 2690 3437
Fragments arranged by size:
4333 3437 2690 1074 660
BanI G'GyrC_C
Cuts at: 1491 7338 9210 9624 9872 9907 1491
Size: 5847 1872 414 248 35 3778
Fragments arranged by size:
5847 3778 1872 414 248 35
BanII G_rGCy'C
Cuts at: 4794 7394 8397 9208 10238 4794
Size: 2600 1003 811 1030 6750
Fragments arranged by size:
6750 2600 1030 1003 811
BbeI G_GCGC'C
Cuts at: 9628 9876 9628
Size: 248 11946
BbsI GAAGACnn'nnnn_
Cuts at: 1270 2618 3557 5855 7621 8634 8983 1270
Size: 1348 939 2298 1766 1013 349 4481
Fragments arranged by size:
4481 2298 1766 1348 1013 939 349
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1945 1979 8061 8095 1945
Size: 34 6082 34 6044
Fragments arranged by size:
6082 6044 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5796 8706 10092 10853 12194 5796
Size: 2910 1386 761 1341 5796
Fragments arranged by size:
5796 2910 1386 1341 761
BclI T'GATC_A
Cuts at: 1533 5505 9714 1533
Size: 3972 4209 4013
Fragments arranged by size:
4209 4013 3972
BfrBI ATG'CAT
Cuts at: 202 590 2086 2543 3336 3813 4970 5406 6801
Size: 388 1496 457 793 477 1157 436 1395
Cuts at: 6801 8749 202
Size: 1948 3647
Fragments arranged by size:
3647 1948 1496 1395 1157 793 477 457
436 388
BglII A'GATC_T
Cuts at: 564 3313 9709 11746 564
Size: 2749 6396 2037 1012
Fragments arranged by size:
6396 2749 2037 1012
BlpI GC'TnA_GC
Cuts at: 7418 9306 7418
Size: 1888 10306
Bme1580I G_kGCm'C
Cuts at: 3264 3686 7200 9819 9912 10968 3264
Size: 422 3514 2619 93 1056 4490
Fragments arranged by size:
4490 3514 2619 1056 422 93
BmgBI CAC'GTC
Cuts at: 2184 3140 2184
Size: 956 11238
BmrI ACTGGGnnnn_n'
Cuts at: 235 2195 2236 2342 7035 9406 9487 9822 11966
Size: 1960 41 106 4693 2371 81 335 2144
Cuts at: 11966 235
Size: 463
Fragments arranged by size:
4693 2371 2144 1960 463 335 106 81
41
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2932 4813 5940 6657 10561 2932
Size: 1881 1127 717 3904 4565
Fragments arranged by size:
4565 3904 1881 1127 717
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 716 2285 2846 2902 3126 3903 5982 7519 8015
Size: 1569 561 56 224 777 2079 1537 496
Cuts at: 8015 9071 716
Size: 1056 3839
Fragments arranged by size:
3839 2079 1569 1537 1056 777 561 496
224 56
Bpu10I CC'TnA_GC
Cuts at: 4556 4565 4641 7388 8591 4556
Size: 9 76 2747 1203 8159
Fragments arranged by size:
8159 2747 1203 76 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1485 2653 2717 3406 5272 8381 9370 10249 10741
Size: 1168 64 689 1866 3109 989 879 492
Cuts at: 10741 11039 1485
Size: 298 2640
Fragments arranged by size:
3109 2640 1866 1168 989 879 689 492
298 64
BsaI GGTCTCn'nnnn_
Cuts at: 4229 4253 11898 4229
Size: 24 7645 4525
Fragments arranged by size:
7645 4525 24
BsaAI yAC'GTr
Cuts at: 5344 9547 5344
Size: 4203 7991
BsaBI GATnn'nnATC
Cuts at: 6 1127 1394 7529 9708 11745 6
Size: 1121 267 6135 2179 2037 455
Fragments arranged by size:
6135 2179 2037 1121 455 267
BsaHI Gr'CG_yC
Cuts at: 5583 9625 9873 10575 12071 5583
Size: 4042 248 702 1496 5706
Fragments arranged by size:
5706 4042 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1267 4943 9383 9904 10582 10856 11003 11414 1267
Size: 3676 4440 521 678 274 147 411 2047
Fragments arranged by size:
4440 3676 2047 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 145 175 4612 4642 7827 7857 8303 8333 8762
Size: 30 4437 30 3185 30 446 30 429
Cuts at: 8762 8792 9076 9106 145
Size: 30 284 30 3233
Fragments arranged by size:
4437 3233 3185 446 429 284 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1086 2282 6643 7480 8324 8646 1086
Size: 1196 4361 837 844 322 4634
Fragments arranged by size:
4634 4361 1196 844 837 322
BseYI C'CCAG_C
Cuts at: 664 1200 4168 9619 10377 10954 11450 664
Size: 536 2968 5451 758 577 496 1408
Fragments arranged by size:
5451 2968 1408 758 577 536 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 100 2225 100
Size: 2125 10069
BsiEI CG_ry'CG
Cuts at: 104 9736 9782 10990 104
Size: 9632 46 1208 1308
Fragments arranged by size:
9632 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3060 3264 3883 4794 5466 5700 7200 7394 7604
Size: 204 619 911 672 234 1500 194 210
Cuts at: 7604 8397 9208 9986 10176 10968 3060
Size: 793 811 778 190 792 4286
Fragments arranged by size:
4286 1500 911 811 793 792 778 672
619 234 210 204 194 190
BsmI GAATG_Cn'
Cuts at: 2152 2731 4003 4270 5163 2152
Size: 579 1272 267 893 9183
Fragments arranged by size:
9183 1272 893 579 267
BsmBI CGTCTCn'nnnn_
Cuts at: 5574 5574
Size: 12194
Bsp1286I G_dGCh'C
Cuts at: 3060 3264 3686 3883 4794 5466 5700 7200 7394
Size: 204 422 197 911 672 234 1500 194
Cuts at: 7394 7604 8397 9208 9819 9912 9986 10176 10238
Size: 210 793 811 611 93 74 190 62
Cuts at: 10238 10968 3060
Size: 730 4286
Fragments arranged by size:
4286 1500 911 811 793 730 672 611
422 234 210 204 197 194 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4943 11414 4943
Size: 6471 5723
BspHI T'CATG_A
Cuts at: 4206 5409 7638 8091 11370 4206
Size: 1203 2229 453 3279 5030
Fragments arranged by size:
5030 3279 2229 1203 453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 122 2921 7624 7786 8736 8764 9244 9458 9760
Size: 2799 4703 162 950 28 480 214 302
Cuts at: 9760 10141 122
Size: 381 2175
Fragments arranged by size:
4703 2799 2175 950 480 381 302 214
162 28
BsrBI CCG'CTC
Cuts at: 50 10486 10540 12024 12189 50
Size: 10436 54 1484 165 55
Fragments arranged by size:
10436 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 597 4163 5048 10106 597
Size: 3566 885 5058 2685
Fragments arranged by size:
5058 3566 2685 885
BsrFI r'CCGG_y
Cuts at: 10192 10373 10582 10192
Size: 181 209 11804
Fragments arranged by size:
11804 209 181
BsrGI T'GTAC_A
Cuts at: 1281 6664 1281
Size: 5383 6811
BssSI C'ACGA_G
Cuts at: 8207 10465 10823 8207
Size: 2258 358 9578
Fragments arranged by size:
9578 2258 358
BstAPI GCAn_nnn'nTGC
Cuts at: 123 3055 7599 7801 9571 123
Size: 2932 4544 202 1770 2746
Fragments arranged by size:
4544 2932 2746 1770 202
BstBI TT'CG_AA
Cuts at: 10555 10555
Size: 12194
BstXI CCAn_nnnn'nTGG
Cuts at: 6986 8767 6986
Size: 1781 10413
BstYI r'GATC_y
Cuts at: 1 462 564 1122 2196 2556 2870 3313 4110
Size: 461 102 558 1074 360 314 443 797
Cuts at: 4110 5905 6529 8944 9219 9686 9709 10044 10290
Size: 1795 624 2415 275 467 23 335 246
Cuts at: 10290 11302 11388 11400 11746 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2415 1795 1074 1012 797 624 558 467
461 449 443 360 346 335 314 275
246 102 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 4219 4219
Size: 12194
BtgI C'CryG_G
Cuts at: 164 1322 1601 6534 7104 7737 8281 164
Size: 1158 279 4933 570 633 544 4077
Fragments arranged by size:
4933 4077 1158 633 570 544 279
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 7600 10144 10325 10488 7600
Size: 2544 181 163 9306
Fragments arranged by size:
9306 2544 181 163
BtsI GCAGTG_nn'
Cuts at: 153 659 1150 3060 5687 7088 8617 9437 153
Size: 506 491 1910 2627 1401 1529 820 2910
Fragments arranged by size:
2910 2627 1910 1529 1401 820 506 491
ClaI AT'CG_AT
Cuts at: 5363 5363
Size: 12194
DraI TTT'AAA
Cuts at: 554 1871 5153 5705 6158 6209 9152 9193 554
Size: 1317 3282 552 453 51 2943 41 3555
Fragments arranged by size:
3555 3282 2943 1317 552 453 51 41
DraIII CAC_nnn'GTG
Cuts at: 123 123
Size: 12194
DrdI GACnn_nn'nnGTC
Cuts at: 9900 10758 9900
Size: 858 11336
EaeI y'GGCC_r
Cuts at: 420 3184 4578 6988 7112 8126 9779 9953 10344
Size: 2764 1394 2410 124 1014 1653 174 391
Cuts at: 10344 10371 11716 420
Size: 27 1345 898
Fragments arranged by size:
2764 2410 1653 1394 1345 1014 898 391
174 124 27
EagI C'GGCC_G
Cuts at: 9779 9779
Size: 12194
EarI CTCTTCn'nnn_
Cuts at: 1253 2303 6320 7102 7440 7700 7775 7876 8378
Size: 1050 4017 782 338 260 75 101 502
Cuts at: 8378 8921 10217 10427 1253
Size: 543 1296 210 3020
Fragments arranged by size:
4017 3020 1296 1050 782 543 502 338
260 210 101 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 729 10402 10712 10858 12004 729
Size: 9673 310 146 1146 919
Fragments arranged by size:
9673 1146 919 310 146
EcoICRI GAG'CTC
Cuts at: 4792 7392 8395 9206 4792
Size: 2600 1003 811 7780
Fragments arranged by size:
7780 2600 1003 811
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 560 716 2285 2846 2902 3126 3193 3903 3998
Size: 156 1569 561 56 224 67 710 95
Cuts at: 3998 5897 5982 6301 6368 6428 7519 8015 8945
Size: 1899 85 319 67 60 1091 496 930
Cuts at: 8945 9071 10019 10451 11198 11466 560
Size: 126 948 432 747 268 1288
Fragments arranged by size:
1899 1569 1288 1091 948 930 747 710
561 496 432 319 268 224 156 126
95 85 67 67 60 56
EcoNI CCTnn'n_nnAGG
Cuts at: 118 118
Size: 12194
EcoO109I rG'GnC_Cy
Cuts at: 3381 7798 9073 9333 3381
Size: 4417 1275 260 6242
Fragments arranged by size:
6242 4417 1275 260
EcoRI G'AATT_C
Cuts at: 1694 7020 8120 9198 1694
Size: 5326 1100 1078 4690
Fragments arranged by size:
5326 4690 1100 1078
EcoRV GAT'ATC
Cuts at: 1105 1907 6774 7990 8031 1105
Size: 802 4867 1216 41 5268
Fragments arranged by size:
5268 4867 1216 802 41
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3223 4158 8375 10213 3223
Size: 935 4217 1838 5204
Fragments arranged by size:
5204 4217 1838 935
FspI TGC'GCA
Cuts at: 9975 9975
Size: 12194
HaeII r_GCGC'y
Cuts at: 189 9628 9876 10898 11877 189
Size: 9439 248 1022 979 506
Fragments arranged by size:
9439 1022 979 506 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1396 1428 1711 1743 2280 2312 2862 2894 3115
Size: 32 283 32 537 32 550 32 221
Cuts at: 3115 3147 3526 3558 4059 4091 4458 4490 4612
Size: 32 379 32 501 32 367 32 122
Cuts at: 4612 4644 5920 5952 6322 6354 6458 6490 7608
Size: 32 1276 32 370 32 104 32 1118
Cuts at: 7608 7640 7718 7750 7774 7806 8349 8379 8381
Size: 32 78 32 24 32 543 30 2
Cuts at: 8381 8411 8958 8990 10036 10066 10068 10098 1396
Size: 30 547 32 1046 30 2 30 3492
Fragments arranged by size:
3492 1276 1118 1046 550 547 543 537
501 379 370 367 283 221 122 104
78 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 30 30 30 30 24 2 2
HincII GTy'rAC
Cuts at: 366 875 1773 2181 2238 2518 2936 8857 9233
Size: 509 898 408 57 280 418 5921 376
Cuts at: 9233 366
Size: 3327
Fragments arranged by size:
5921 3327 898 509 418 408 376 280
57
HindIII A'AGCT_T
Cuts at: 6945 7820 6945
Size: 875 11319
HpaI GTT'AAC
Cuts at: 366 366
Size: 12194
KasI G'GCGC_C
Cuts at: 9624 9872 9624
Size: 248 11946
KpnI G_GTAC'C
Cuts at: 1495 9214 1495
Size: 7719 4475
MfeI C'AATT_G
Cuts at: 4652 5237 7594 4652
Size: 585 2357 9252
Fragments arranged by size:
9252 2357 585
MluI A'CGCG_T
Cuts at: 11627 11627
Size: 12194
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1048 4564 5822 6077 7746 8392 8944 10865 11049
Size: 3516 1258 255 1669 646 552 1921 184
Cuts at: 11049 1048
Size: 2193
Fragments arranged by size:
3516 2193 1921 1669 1258 646 552 255
184
MscI TGG'CCA
Cuts at: 422 3186 4580 6990 7114 8128 9955 422
Size: 2764 1394 2410 124 1014 1827 2661
Fragments arranged by size:
2764 2661 2410 1827 1394 1014 124
MslI CAynn'nnrTG
Cuts at: 207 1160 1747 1828 1919 2027 2081 3404 3787
Size: 953 587 81 91 108 54 1323 383
Cuts at: 3787 5095 5401 5617 7081 8207 8765 10183 10310
Size: 1308 306 216 1464 1126 558 1418 127
Cuts at: 10310 207
Size: 2091
Fragments arranged by size:
2091 1464 1418 1323 1308 1126 953 587
558 383 306 216 127 108 91 81
54
MspA1I CmG'CkG
Cuts at: 721 1525 8224 8674 9305 9619 9979 10992 11237
Size: 804 6699 450 631 314 360 1013 245
Cuts at: 11237 721
Size: 1678
Fragments arranged by size:
6699 1678 1013 804 631 450 360 314
245
NaeI GCC'GGC
Cuts at: 10375 10375
Size: 12194
NarI GG'CG_CC
Cuts at: 9625 9873 9625
Size: 248 11946
NcoI C'CATG_G
Cuts at: 1322 1601 6534 7104 7737 8281 1322
Size: 279 4933 570 633 544 5235
Fragments arranged by size:
5235 4933 633 570 544 279
NdeI CA'TA_TG
Cuts at: 4478 5618 8787 8838 4478
Size: 1140 3169 51 7834
Fragments arranged by size:
7834 3169 1140 51
NgoMIV G'CCGG_C
Cuts at: 10373 10373
Size: 12194
NsiI A_TGCA'T
Cuts at: 204 592 2088 2545 3338 3815 4972 5408 6803
Size: 388 1496 457 793 477 1157 436 1395
Cuts at: 6803 8751 204
Size: 1948 3647
Fragments arranged by size:
3647 1948 1496 1395 1157 793 477 457
436 388
NspI r_CATG'y
Cuts at: 1159 1630 1918 2390 2760 3053 3340 3480 3817
Size: 471 288 472 370 293 287 140 337
Cuts at: 3817 4564 4875 6206 7204 8206 8280 8589 9019
Size: 747 311 1331 998 1002 74 309 430
Cuts at: 9019 9247 9747 10654 1159
Size: 228 500 907 2699
Fragments arranged by size:
2699 1331 1002 998 907 747 500 472
471 430 370 337 311 309 293 288
287 228 140 74
PciI A'CATG_T
Cuts at: 2756 3476 4871 7200 10650 2756
Size: 720 1395 2329 3450 4300
Fragments arranged by size:
4300 3450 2329 1395 720
PflMI CCAn_nnn'nTGG
Cuts at: 7110 8287 8578 7110
Size: 1177 291 10726
Fragments arranged by size:
10726 1177 291
PfoI T'CCnGG_A
Cuts at: 3773 7535 7992 3773
Size: 3762 457 7975
Fragments arranged by size:
7975 3762 457
PmeI GTTT'AAAC
Cuts at: 9193 9193
Size: 12194
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5553 5585 11333 11365 5553
Size: 32 5748 32 6382
Fragments arranged by size:
6382 5748 32 32
PpuMI rG'GwC_Cy
Cuts at: 3381 7798 3381
Size: 4417 7777
PshAI GACnn'nnGTC
Cuts at: 8852 8852
Size: 12194
PsiI TTA'TAA
Cuts at: 3290 3859 3290
Size: 569 11625
PspXI vC'TCGA_Gb
Cuts at: 800 800
Size: 12194
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3906 3938 5802 5834 3906
Size: 32 1864 32 10266
Fragments arranged by size:
10266 1864 32 32
PstI C_TGCA'G
Cuts at: 958 5202 5325 7797 9241 9926 958
Size: 4244 123 2472 1444 685 3226
Fragments arranged by size:
4244 3226 2472 1444 685 123
PvuI CG_AT'CG
Cuts at: 104 104
Size: 12194
PvuII CAG'CTG
Cuts at: 721 1525 8224 8674 9619 9979 721
Size: 804 6699 450 945 360 2936
Fragments arranged by size:
6699 2936 945 804 450 360
RsrII CG'GwC_CG
Cuts at: 10389 10389
Size: 12194
SacI G_AGCT'C
Cuts at: 4794 7394 8397 9208 4794
Size: 2600 1003 811 7780
Fragments arranged by size:
7780 2600 1003 811
SalI G'TCGA_C
Cuts at: 9231 9231
Size: 12194
SapI GCTCTTCn'nnn_
Cuts at: 6320 10217 10427 6320
Size: 3897 210 8087
Fragments arranged by size:
8087 3897 210
SbfI CC_TGCA'GG
Cuts at: 9241 9241
Size: 12194
ScaI AGT'ACT
Cuts at: 1550 6911 8932 11711 1550
Size: 5361 2021 2779 2033
Fragments arranged by size:
5361 2779 2033 2021
SexAI A'CCwGG_T
Cuts at: 119 3234 119
Size: 3115 9079
SfcI C'TryA_G
Cuts at: 33 954 3245 3572 3731 5198 5321 5531 6355
Size: 921 2291 327 159 1467 123 210 824
Cuts at: 6355 6861 7793 8669 9237 9922 10915 11106 33
Size: 506 932 876 568 685 993 191 1121
Fragments arranged by size:
2291 1467 1121 993 932 921 876 824
685 568 506 327 210 191 159 123
SfoI GGC'GCC
Cuts at: 9626 9874 9626
Size: 248 11946
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 12194
SmaI CCC'GGG
Cuts at: 9216 9216
Size: 12194
SmlI C'TyrA_G
Cuts at: 800 1500 2632 2732 3385 5251 8396 9349 10264
Size: 700 1132 100 653 1866 3145 953 915
Cuts at: 10264 10756 11018 800
Size: 492 262 1976
Fragments arranged by size:
3145 1976 1866 1132 953 915 700 653
492 262 100
SnaBI TAC'GTA
Cuts at: 5344 5344
Size: 12194
SpeI A'CTAG_T
Cuts at: 67 5806 7251 67
Size: 5739 1445 5010
Fragments arranged by size:
5739 5010 1445
SphI G_CATG'C
Cuts at: 1159 2390 3053 4564 8589 9247 9747 1159
Size: 1231 663 1511 4025 658 500 3606
Fragments arranged by size:
4025 3606 1511 1231 663 658 500
SspI AAT'ATT
Cuts at: 279 2139 9042 279
Size: 1860 6903 3431
Fragments arranged by size:
6903 3431 1860
StuI AGG'CCT
Cuts at: 3243 4811 3243
Size: 1568 10626
StyI C'CwwG_G
Cuts at: 759 847 1322 1601 2646 4133 4698 4806 6534
Size: 88 475 279 1045 1487 565 108 1728
Cuts at: 6534 7104 7737 8281 9328 9682 759
Size: 570 633 544 1047 354 3271
Fragments arranged by size:
3271 1728 1487 1047 1045 633 570 565
544 475 354 279 108 88
SwaI ATTT'AAAT
Cuts at: 554 554
Size: 12194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2350 4915 9777 9910 10369 10578 2350
Size: 2565 4862 133 459 209 3966
Fragments arranged by size:
4862 3966 2565 459 209 133
TatI w'GTAC_w
Cuts at: 867 1281 1548 1671 3699 4056 4532 6664 6909
Size: 414 267 123 2028 357 476 2132 245
Cuts at: 6909 8293 8930 11709 867
Size: 1384 637 2779 1352
Fragments arranged by size:
2779 2132 2028 1384 1352 637 476 414
357 267 245 123
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 145 177 1197 1229 5119 5151 8303 8335 9038
Size: 32 1020 32 3890 32 3152 32 703
Cuts at: 9038 9070 9076 9108 11711 11743 145
Size: 32 6 32 2603 32 596
Fragments arranged by size:
3890 3152 2603 1020 703 596 32 32
32 32 32 32 32 6
Tth111I GACn'n_nGTC
Cuts at: 9991 9991
Size: 12194
XbaI T'CTAG_A
Cuts at: 3544 9225 3544
Size: 5681 6513
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 522 2355 7863 8603 9689 522
Size: 1833 5508 740 1086 3027
Fragments arranged by size:
5508 3027 1833 1086 740
XhoI C'TCGA_G
Cuts at: 800 800
Size: 12194
XmaI C'CCGG_G
Cuts at: 9214 9214
Size: 12194
XmnI GAAnn'nnTTC
Cuts at: 1005 1022 1550 5364 6452 7712 7916 8358 1005
Size: 17 528 3814 1088 1260 204 442 4841
Fragments arranged by size:
4841 3814 1260 1088 528 442 204 17
ZraI GAC'GTC
Cuts at: 5584 5584
Size: 12194
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AflII AhdI AloI ApaI AscI BbvCI BglI BmtI
BsiWI BssHII BstEII BstZ17I CspCI FseI FspAI NheI
NotI NruI PacI PasI PmlI PspOMI SacII SanDI
SfiI SgrAI SrfI