Restriction map for
pF1KB6996
(Circular) (Six-base) MAPSORT of: pF1KB6996.seq Check: 3672 from: 1 to: 3875
pF1KB6996 3875 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 913 913
Size: 3875
Acc65I G'GTAC_C
Cuts at: 891 891
Size: 3875
AclI AA'CG_TT
Cuts at: 3715 3715
Size: 3875
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 617 1700 2132 2879 3147 563
Size: 54 1083 432 747 268 1291
Fragments arranged by size:
1291 1083 747 432 268 54
AflIII A'CryG_T
Cuts at: 281 1227 2331 3308 281
Size: 946 1104 977 848
Fragments arranged by size:
1104 977 946 848
AgeI A'CCGG_T
Cuts at: 2263 2263
Size: 3875
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 329 361 329
Size: 32 3843
AlwNI CAG_nnn'CTG
Cuts at: 216 595 1180 2747 216
Size: 379 585 1567 1344
Fragments arranged by size:
1567 1344 585 379
ApaLI G'TGCA_C
Cuts at: 2645 2645
Size: 3875
ApoI r'AATT_y
Cuts at: 498 776 879 3228 3484 498
Size: 278 103 2349 256 889
Fragments arranged by size:
2349 889 278 256 103
AseI AT'TA_AT
Cuts at: 20 20
Size: 3875
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
AvaI C'yCGr_G
Cuts at: 895 895
Size: 3875
BamHI G'GATC_C
Cuts at: 900 900
Size: 3875
BanI G'GyrC_C
Cuts at: 891 1305 1553 1588 891
Size: 414 248 35 3178
Fragments arranged by size:
3178 414 248 35
BanII G_rGCy'C
Cuts at: 889 1919 889
Size: 1030 2845
BbeI G_GCGC'C
Cuts at: 1309 1557 1309
Size: 248 3627
BbsI GAAGACnn'nnnn_
Cuts at: 435 435
Size: 3875
BciVI GTATCCnnnnn_n'
Cuts at: 1773 2534 3875 1773
Size: 761 1341 1773
Fragments arranged by size:
1773 1341 761
BclI T'GATC_A
Cuts at: 1395 1395
Size: 3875
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3875
BglII A'GATC_T
Cuts at: 1390 3427 1390
Size: 2037 1838
BlpI GC'TnA_GC
Cuts at: 987 987
Size: 3875
Bme1580I G_kGCm'C
Cuts at: 1500 1593 2649 1500
Size: 93 1056 2726
Fragments arranged by size:
2726 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 265 1087 1168 1503 3647 265
Size: 822 81 335 2144 493
Fragments arranged by size:
2144 822 493 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2242 2242
Size: 3875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 709
Size: 3875
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 1051 1930 2422 2720 370
Size: 681 879 492 298 1525
Fragments arranged by size:
1525 879 681 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3579 3579
Size: 3875
BsaAI yAC'GTr
Cuts at: 1228 1228
Size: 3875
BsaBI GATnn'nnATC
Cuts at: 6 1389 3426 6
Size: 1383 2037 455
Fragments arranged by size:
2037 1383 455
BsaHI Gr'CG_yC
Cuts at: 1306 1554 2256 3752 1306
Size: 248 702 1496 1429
Fragments arranged by size:
1496 1429 702 248
BsaWI w'CCGG_w
Cuts at: 804 1064 1585 2263 2537 2684 3095 804
Size: 260 521 678 274 147 411 1584
Fragments arranged by size:
1584 678 521 411 274 260 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 665 695 665
Size: 30 3845
BseYI C'CCAG_C
Cuts at: 131 378 1300 2058 2635 3131 131
Size: 247 922 758 577 496 875
Fragments arranged by size:
922 875 758 577 496 247
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 682
Size: 3875
BsiEI CG_ry'CG
Cuts at: 104 1417 1463 2671 104
Size: 1313 46 1208 1308
Fragments arranged by size:
1313 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 889 1667 1857 2649 889
Size: 778 190 792 2115
Fragments arranged by size:
2115 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 889 1500 1593 1667 1857 1919 2649 889
Size: 611 93 74 190 62 730 2115
Fragments arranged by size:
2115 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 804 3095 804
Size: 2291 1584
BspHI T'CATG_A
Cuts at: 3051 3051
Size: 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 261 925 1139 1441 1822 261
Size: 664 214 302 381 2314
Fragments arranged by size:
2314 664 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2167 2221 3705 3870 50
Size: 2117 54 1484 165 55
Fragments arranged by size:
2117 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 626 1787 626
Size: 1161 2714
BsrFI r'CCGG_y
Cuts at: 1873 2054 2263 1873
Size: 181 209 3485
Fragments arranged by size:
3485 209 181
BsrGI T'GTAC_A
Cuts at: 228 711 228
Size: 483 3392
BssSI C'ACGA_G
Cuts at: 2146 2504 2146
Size: 358 3517
BstAPI GCAn_nnn'nTGC
Cuts at: 1252 1252
Size: 3875
BstBI TT'CG_AA
Cuts at: 2236 2236
Size: 3875
BstYI r'GATC_y
Cuts at: 1 592 900 1367 1390 1725 1971 2983 3069
Size: 591 308 467 23 335 246 1012 86
Cuts at: 3069 3081 3427 1
Size: 12 346 449
Fragments arranged by size:
1012 591 467 449 346 335 308 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 362 107
Size: 255 3620
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1825 2006 2169 1825
Size: 181 163 3531
Fragments arranged by size:
3531 181 163
BtsI GCAGTG_nn'
Cuts at: 1118 1118
Size: 3875
DraI TTT'AAA
Cuts at: 874 874
Size: 3875
DraIII CAC_nnn'GTG
Cuts at: 273 273
Size: 3875
DrdI GACnn_nn'nnGTC
Cuts at: 1581 2439 1581
Size: 858 3017
EaeI y'GGCC_r
Cuts at: 119 652 1460 1634 2025 2052 3397 119
Size: 533 808 174 391 27 1345 597
Fragments arranged by size:
1345 808 597 533 391 174 27
EagI C'GGCC_G
Cuts at: 1460 1460
Size: 3875
EarI CTCTTCn'nnn_
Cuts at: 1898 2108 1898
Size: 210 3665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2083 2393 2539 3685 2083
Size: 310 146 1146 2273
Fragments arranged by size:
2273 1146 310 146
EcoICRI GAG'CTC
Cuts at: 887 887
Size: 3875
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 617 709 1700 2132 2879 3147 563
Size: 54 92 991 432 747 268 1291
Fragments arranged by size:
1291 991 747 432 268 92 54
EcoO109I rG'GnC_Cy
Cuts at: 1014 1014
Size: 3875
EcoRI G'AATT_C
Cuts at: 776 879 776
Size: 103 3772
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1894 1894
Size: 3875
FspI TGC'GCA
Cuts at: 163 1656 163
Size: 1493 2382
HaeII r_GCGC'y
Cuts at: 1309 1557 2579 3558 1309
Size: 248 1022 979 1626
Fragments arranged by size:
1626 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 826 858 1717 1747 1749 1779 826
Size: 32 859 30 2 30 2922
Fragments arranged by size:
2922 859 32 30 30 2
HincII GTy'rAC
Cuts at: 914 914
Size: 3875
KasI G'GCGC_C
Cuts at: 1305 1553 1305
Size: 248 3627
KpnI G_GTAC'C
Cuts at: 895 895
Size: 3875
MluI A'CGCG_T
Cuts at: 3308 3308
Size: 3875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2546 2730 2546
Size: 184 3691
MscI TGG'CCA
Cuts at: 1636 1636
Size: 3875
MslI CAynn'nnrTG
Cuts at: 1864 1991 1864
Size: 127 3748
MspA1I CmG'CkG
Cuts at: 382 717 743 986 1300 1660 2673 2918 382
Size: 335 26 243 314 360 1013 245 1339
Fragments arranged by size:
1339 1013 360 335 314 245 243 26
NaeI GCC'GGC
Cuts at: 2056 2056
Size: 3875
NarI GG'CG_CC
Cuts at: 1306 1554 1306
Size: 248 3627
NcoI C'CATG_G
Cuts at: 107 362 107
Size: 255 3620
NdeI CA'TA_TG
Cuts at: 179 179
Size: 3875
NgoMIV G'CCGG_C
Cuts at: 2054 2054
Size: 3875
NspI r_CATG'y
Cuts at: 285 789 928 1428 2335 285
Size: 504 139 500 907 1825
Fragments arranged by size:
1825 907 504 500 139
PciI A'CATG_T
Cuts at: 281 2331 281
Size: 2050 1825
PmeI GTTT'AAAC
Cuts at: 874 874
Size: 3875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3014 3046 3014
Size: 32 3843
PstI C_TGCA'G
Cuts at: 258 387 794 922 1607 258
Size: 129 407 128 685 2526
Fragments arranged by size:
2526 685 407 129 128
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3875
PvuII CAG'CTG
Cuts at: 382 717 743 1300 1660 382
Size: 335 26 557 360 2597
Fragments arranged by size:
2597 557 360 335 26
RsrII CG'GwC_CG
Cuts at: 2070 2070
Size: 3875
SacI G_AGCT'C
Cuts at: 889 889
Size: 3875
SalI G'TCGA_C
Cuts at: 912 912
Size: 3875
SapI GCTCTTCn'nnn_
Cuts at: 1898 2108 1898
Size: 210 3665
SbfI CC_TGCA'GG
Cuts at: 922 922
Size: 3875
ScaI AGT'ACT
Cuts at: 3392 3392
Size: 3875
SfcI C'TryA_G
Cuts at: 33 254 383 790 918 1603 2596 2787 33
Size: 221 129 407 128 685 993 191 1121
Fragments arranged by size:
1121 993 685 407 221 191 129 128
SfoI GGC'GCC
Cuts at: 1307 1555 1307
Size: 248 3627
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
SmaI CCC'GGG
Cuts at: 897 897
Size: 3875
SmlI C'TyrA_G
Cuts at: 349 1030 1945 2437 2699 349
Size: 681 915 492 262 1525
Fragments arranged by size:
1525 915 681 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3875
SphI G_CATG'C
Cuts at: 928 1428 928
Size: 500 3375
StyI C'CwwG_G
Cuts at: 107 362 1009 1363 107
Size: 255 647 354 2619
Fragments arranged by size:
2619 647 354 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 284 1458 1591 2050 2259 284
Size: 1174 133 459 209 1900
Fragments arranged by size:
1900 1174 459 209 133
TatI w'GTAC_w
Cuts at: 228 711 3390 228
Size: 483 2679 713
Fragments arranged by size:
2679 713 483
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3392 3424 3392
Size: 32 3843
Tth111I GACn'n_nGTC
Cuts at: 1672 1672
Size: 3875
XbaI T'CTAG_A
Cuts at: 906 906
Size: 3875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1370 1370
Size: 3875
XmaI C'CCGG_G
Cuts at: 895 895
Size: 3875
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BseRI BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI