Restriction map for
pF1KB6991
(Circular) (Six-base) MAPSORT of: pF1KB6991.seq Check: 4252 from: 1 to: 3776
pF1KB6991 3776 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 756 756
Size: 3776
AccI GT'mk_AC
Cuts at: 158 381 814 158
Size: 223 433 3120
Fragments arranged by size:
3120 433 223
Acc65I G'GTAC_C
Cuts at: 792 792
Size: 3776
AclI AA'CG_TT
Cuts at: 586 3616 586
Size: 3030 746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 391 1601 2033 2780 3048 179
Size: 212 1210 432 747 268 907
Fragments arranged by size:
1210 907 747 432 268 212
AfeI AGC'GCT
Cuts at: 472 472
Size: 3776
AflIII A'CryG_T
Cuts at: 1128 2232 3209 1128
Size: 1104 977 1695
Fragments arranged by size:
1695 1104 977
AgeI A'CCGG_T
Cuts at: 2164 2164
Size: 3776
AlwNI CAG_nnn'CTG
Cuts at: 214 651 1081 2648 214
Size: 437 430 1567 1342
Fragments arranged by size:
1567 1342 437 430
ApaI G_GGCC'C
Cuts at: 292 292
Size: 3776
ApaLI G'TGCA_C
Cuts at: 2546 2546
Size: 3776
ApoI r'AATT_y
Cuts at: 780 3129 3385 780
Size: 2349 256 1171
Fragments arranged by size:
2349 1171 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3776
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3776
AvaI C'yCGr_G
Cuts at: 246 796 246
Size: 550 3226
BamHI G'GATC_C
Cuts at: 801 801
Size: 3776
BanI G'GyrC_C
Cuts at: 591 792 1206 1454 1489 591
Size: 201 414 248 35 2878
Fragments arranged by size:
2878 414 248 201 35
BanII G_rGCy'C
Cuts at: 292 790 1820 292
Size: 498 1030 2248
Fragments arranged by size:
2248 1030 498
BbeI G_GCGC'C
Cuts at: 1210 1458 1210
Size: 248 3528
BbvCI CC'TCA_GC
Cuts at: 207 207
Size: 3776
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3742
BciVI GTATCCnnnnn_n'
Cuts at: 1674 2435 3776 1674
Size: 761 1341 1674
Fragments arranged by size:
1674 1341 761
BclI T'GATC_A
Cuts at: 1296 1296
Size: 3776
BglI GCCn_nnn'nGGC
Cuts at: 761 761
Size: 3776
BglII A'GATC_T
Cuts at: 1291 3328 1291
Size: 2037 1739
BlpI GC'TnA_GC
Cuts at: 888 888
Size: 3776
Bme1580I G_kGCm'C
Cuts at: 292 596 766 1401 1494 2550 292
Size: 304 170 635 93 1056 1518
Fragments arranged by size:
1518 1056 635 304 170 93
BmrI ACTGGGnnnn_n'
Cuts at: 988 1069 1404 3548 988
Size: 81 335 2144 1216
Fragments arranged by size:
2144 1216 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 3776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 538
Size: 3776
Bpu10I CC'TnA_GC
Cuts at: 207 207
Size: 3776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 952 1831 2323 2621 952
Size: 879 492 298 2107
Fragments arranged by size:
2107 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3480 3480
Size: 3776
BsaAI yAC'GTr
Cuts at: 1129 1129
Size: 3776
BsaBI GATnn'nnATC
Cuts at: 6 358 1290 3327 6
Size: 352 932 2037 455
Fragments arranged by size:
2037 932 455 352
BsaHI Gr'CG_yC
Cuts at: 1207 1455 2157 3653 1207
Size: 248 702 1496 1330
Fragments arranged by size:
1496 1330 702 248
BsaWI w'CCGG_w
Cuts at: 965 1486 2164 2438 2585 2996 965
Size: 521 678 274 147 411 1745
Fragments arranged by size:
1745 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 182 254 1201 1959 2536 3032 182
Size: 72 947 758 577 496 926
Fragments arranged by size:
947 926 758 577 496 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 640 361
Size: 279 3497
BsiEI CG_ry'CG
Cuts at: 104 1318 1364 2572 104
Size: 1214 46 1208 1308
Fragments arranged by size:
1308 1214 1208 46
BsiHKAI G_wGCw'C
Cuts at: 790 1568 1758 2550 790
Size: 778 190 792 2016
Fragments arranged by size:
2016 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 123 123
Size: 3776
Bsp1286I G_dGCh'C
Cuts at: 292 596 766 790 1401 1494 1568 1758 1820
Size: 304 170 24 611 93 74 190 62
Cuts at: 1820 2550 292
Size: 730 1518
Fragments arranged by size:
1518 730 611 304 190 170 93 74
62 24
BspEI T'CCGG_A
Cuts at: 2996 2996
Size: 3776
BspHI T'CATG_A
Cuts at: 2952 2952
Size: 3776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 756 826 1040 1342 1723 756
Size: 70 214 302 381 2809
Fragments arranged by size:
2809 381 302 214 70
BsrBI CCG'CTC
Cuts at: 50 302 2068 2122 3606 3771 50
Size: 252 1766 54 1484 165 55
Fragments arranged by size:
1766 1484 252 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1688 1688
Size: 3776
BsrFI r'CCGG_y
Cuts at: 1774 1955 2164 1774
Size: 181 209 3386
Fragments arranged by size:
3386 209 181
BssSI C'ACGA_G
Cuts at: 2047 2405 2047
Size: 358 3418
BstAPI GCAn_nnn'nTGC
Cuts at: 748 1153 748
Size: 405 3371
BstBI TT'CG_AA
Cuts at: 2137 2137
Size: 3776
BstYI r'GATC_y
Cuts at: 1 801 1268 1291 1626 1872 2884 2970 2982
Size: 800 467 23 335 246 1012 86 12
Cuts at: 2982 3328 1
Size: 346 449
Fragments arranged by size:
1012 800 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1726 1907 2070 1726
Size: 181 163 3432
Fragments arranged by size:
3432 181 163
BtsI GCAGTG_nn'
Cuts at: 459 623 1019 459
Size: 164 396 3216
Fragments arranged by size:
3216 396 164
DraI TTT'AAA
Cuts at: 775 775
Size: 3776
DraIII CAC_nnn'GTG
Cuts at: 508 508
Size: 3776
DrdI GACnn_nn'nnGTC
Cuts at: 1482 2340 1482
Size: 858 2918
EaeI y'GGCC_r
Cuts at: 1361 1535 1926 1953 3298 1361
Size: 174 391 27 1345 1839
Fragments arranged by size:
1839 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1361 1361
Size: 3776
EarI CTCTTCn'nnn_
Cuts at: 485 1799 2009 485
Size: 1314 210 2252
Fragments arranged by size:
2252 1314 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1984 2294 2440 3586 1984
Size: 310 146 1146 2174
Fragments arranged by size:
2174 1146 310 146
EcoICRI GAG'CTC
Cuts at: 788 788
Size: 3776
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 391 538 1601 2033 2780 3048 179
Size: 212 147 1063 432 747 268 907
Fragments arranged by size:
1063 907 747 432 268 212 147
EcoO109I rG'GnC_Cy
Cuts at: 226 250 289 915 226
Size: 24 39 626 3087
Fragments arranged by size:
3087 626 39 24
EcoRI G'AATT_C
Cuts at: 780 780
Size: 3776
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1795 1795
Size: 3776
FspI TGC'GCA
Cuts at: 1557 1557
Size: 3776
HaeII r_GCGC'y
Cuts at: 474 1210 1458 2480 3459 474
Size: 736 248 1022 979 791
Fragments arranged by size:
1022 979 791 736 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 120 152 535 567 1618 1648 1650 1680 120
Size: 32 383 32 1051 30 2 30 2216
Fragments arranged by size:
2216 1051 383 32 32 30 30 2
HincII GTy'rAC
Cuts at: 815 815
Size: 3776
HindIII A'AGCT_T
Cuts at: 191 191
Size: 3776
KasI G'GCGC_C
Cuts at: 1206 1454 1206
Size: 248 3528
KpnI G_GTAC'C
Cuts at: 796 796
Size: 3776
MluI A'CGCG_T
Cuts at: 3209 3209
Size: 3776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 607 2447 2631 607
Size: 1840 184 1752
Fragments arranged by size:
1840 1752 184
MscI TGG'CCA
Cuts at: 1537 1537
Size: 3776
MslI CAynn'nnrTG
Cuts at: 1765 1892 1765
Size: 127 3649
MspA1I CmG'CkG
Cuts at: 199 211 369 516 887 1201 1561 2574 2819
Size: 12 158 147 371 314 360 1013 245
Cuts at: 2819 199
Size: 1156
Fragments arranged by size:
1156 1013 371 360 314 245 158 147
12
NaeI GCC'GGC
Cuts at: 1957 1957
Size: 3776
NarI GG'CG_CC
Cuts at: 1207 1455 1207
Size: 248 3528
NgoMIV G'CCGG_C
Cuts at: 1955 1955
Size: 3776
NspI r_CATG'y
Cuts at: 829 1329 2236 829
Size: 500 907 2369
Fragments arranged by size:
2369 907 500
PasI CC'CwG_GG
Cuts at: 757 757
Size: 3776
PciI A'CATG_T
Cuts at: 2232 2232
Size: 3776
PflMI CCAn_nnn'nTGG
Cuts at: 751 751
Size: 3776
PmeI GTTT'AAAC
Cuts at: 775 775
Size: 3776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2915 2947 2915
Size: 32 3744
PpuMI rG'GwC_Cy
Cuts at: 226 250 226
Size: 24 3752
PsiI TTA'TAA
Cuts at: 350 350
Size: 3776
PspOMI G'GGCC_C
Cuts at: 288 288
Size: 3776
PstI C_TGCA'G
Cuts at: 314 823 1508 314
Size: 509 685 2582
Fragments arranged by size:
2582 685 509
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3776
PvuII CAG'CTG
Cuts at: 211 369 516 1201 1561 211
Size: 158 147 685 360 2426
Fragments arranged by size:
2426 685 360 158 147
RsrII CG'GwC_CG
Cuts at: 1971 1971
Size: 3776
SacI G_AGCT'C
Cuts at: 790 790
Size: 3776
SalI G'TCGA_C
Cuts at: 813 813
Size: 3776
SanDI GG'GwC_CC
Cuts at: 250 250
Size: 3776
SapI GCTCTTCn'nnn_
Cuts at: 1799 2009 1799
Size: 210 3566
SbfI CC_TGCA'GG
Cuts at: 823 823
Size: 3776
ScaI AGT'ACT
Cuts at: 3293 3293
Size: 3776
SfcI C'TryA_G
Cuts at: 33 310 382 819 1504 2497 2688 33
Size: 277 72 437 685 993 191 1121
Fragments arranged by size:
1121 993 685 437 277 191 72
SfoI GGC'GCC
Cuts at: 1208 1456 1208
Size: 248 3528
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3776
SmaI CCC'GGG
Cuts at: 248 798 248
Size: 550 3226
SmlI C'TyrA_G
Cuts at: 931 1846 2338 2600 931
Size: 915 492 262 2107
Fragments arranged by size:
2107 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3776
SphI G_CATG'C
Cuts at: 829 1329 829
Size: 500 3276
StyI C'CwwG_G
Cuts at: 683 910 1264 683
Size: 227 354 3195
Fragments arranged by size:
3195 354 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1359 1492 1951 2160 1359
Size: 133 459 209 2975
Fragments arranged by size:
2975 459 209 133
TatI w'GTAC_w
Cuts at: 3291 3291
Size: 3776
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3293 3325 3293
Size: 32 3744
Tth111I GACn'n_nGTC
Cuts at: 134 1573 134
Size: 1439 2337
XbaI T'CTAG_A
Cuts at: 807 807
Size: 3776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1271 1271
Size: 3776
XmaI C'CCGG_G
Cuts at: 246 796 246
Size: 550 3226
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BfrBI BmgBI BmtI BsaXI BseRI BsiWI
BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI EcoNI EcoRV FseI FspAI HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PshAI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI