Restriction map for
pF1KB6985
(Circular) (Six-base) MAPSORT of: pF1KB6985.seq Check: 5859 from: 1 to: 3770
pF1KB6985 3770 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 684 684
Size: 3770
AccI GT'mk_AC
Cuts at: 808 808
Size: 3770
Acc65I G'GTAC_C
Cuts at: 786 786
Size: 3770
AclI AA'CG_TT
Cuts at: 3610 3610
Size: 3770
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 746 1595 2027 2774 3042 746
Size: 849 432 747 268 1474
Fragments arranged by size:
1474 849 747 432 268
AflIII A'CryG_T
Cuts at: 1122 2226 3203 1122
Size: 1104 977 1689
Fragments arranged by size:
1689 1104 977
AgeI A'CCGG_T
Cuts at: 2158 2158
Size: 3770
AlwNI CAG_nnn'CTG
Cuts at: 670 1075 2642 670
Size: 405 1567 1798
Fragments arranged by size:
1798 1567 405
ApaLI G'TGCA_C
Cuts at: 2540 2540
Size: 3770
ApoI r'AATT_y
Cuts at: 736 774 3123 3379 736
Size: 38 2349 256 1127
Fragments arranged by size:
2349 1127 256 38
AseI AT'TA_AT
Cuts at: 20 20
Size: 3770
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3770
AvaI C'yCGr_G
Cuts at: 790 790
Size: 3770
BamHI G'GATC_C
Cuts at: 795 795
Size: 3770
BanI G'GyrC_C
Cuts at: 568 786 1200 1448 1483 568
Size: 218 414 248 35 2855
Fragments arranged by size:
2855 414 248 218 35
BanII G_rGCy'C
Cuts at: 784 1814 784
Size: 1030 2740
BbeI G_GCGC'C
Cuts at: 1204 1452 1204
Size: 248 3522
BbsI GAAGACnn'nnnn_
Cuts at: 647 647
Size: 3770
BciVI GTATCCnnnnn_n'
Cuts at: 1668 2429 3770 1668
Size: 761 1341 1668
Fragments arranged by size:
1668 1341 761
BclI T'GATC_A
Cuts at: 1290 1290
Size: 3770
BglII A'GATC_T
Cuts at: 1285 3322 1285
Size: 2037 1733
BlpI GC'TnA_GC
Cuts at: 227 882 227
Size: 655 3115
Bme1580I G_kGCm'C
Cuts at: 1395 1488 2544 1395
Size: 93 1056 2621
Fragments arranged by size:
2621 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 448 982 1063 1398 3542 448
Size: 534 81 335 2144 676
Fragments arranged by size:
2144 676 534 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 3770
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1825 2317 2615 946
Size: 879 492 298 2101
Fragments arranged by size:
2101 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3474 3474
Size: 3770
BsaAI yAC'GTr
Cuts at: 1123 1123
Size: 3770
BsaBI GATnn'nnATC
Cuts at: 6 1284 3321 6
Size: 1278 2037 455
Fragments arranged by size:
2037 1278 455
BsaHI Gr'CG_yC
Cuts at: 1201 1449 2151 3647 1201
Size: 248 702 1496 1324
Fragments arranged by size:
1496 1324 702 248
BsaWI w'CCGG_w
Cuts at: 959 1480 2158 2432 2579 2990 959
Size: 521 678 274 147 411 1739
Fragments arranged by size:
1739 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 713 743 713
Size: 30 3740
BseYI C'CCAG_C
Cuts at: 151 1195 1953 2530 3026 151
Size: 1044 758 577 496 895
Fragments arranged by size:
1044 895 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 656 656
Size: 3770
BsiEI CG_ry'CG
Cuts at: 104 1312 1358 2566 104
Size: 1208 46 1208 1308
Fragments arranged by size:
1308 1208 1208 46
BsiHKAI G_wGCw'C
Cuts at: 784 1562 1752 2544 784
Size: 778 190 792 2010
Fragments arranged by size:
2010 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 784 1395 1488 1562 1752 1814 2544 784
Size: 611 93 74 190 62 730 2010
Fragments arranged by size:
2010 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2990 2990
Size: 3770
BspHI T'CATG_A
Cuts at: 2946 2946
Size: 3770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 684 820 1034 1336 1717 684
Size: 136 214 302 381 2737
Fragments arranged by size:
2737 381 302 214 136
BsrBI CCG'CTC
Cuts at: 50 2062 2116 3600 3765 50
Size: 2012 54 1484 165 55
Fragments arranged by size:
2012 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1682 1682
Size: 3770
BsrFI r'CCGG_y
Cuts at: 1768 1949 2158 1768
Size: 181 209 3380
Fragments arranged by size:
3380 209 181
BssSI C'ACGA_G
Cuts at: 2041 2399 2041
Size: 358 3412
BstAPI GCAn_nnn'nTGC
Cuts at: 670 1147 670
Size: 477 3293
BstBI TT'CG_AA
Cuts at: 2131 2131
Size: 3770
BstYI r'GATC_y
Cuts at: 1 752 795 1262 1285 1620 1866 2878 2964
Size: 751 43 467 23 335 246 1012 86
Cuts at: 2964 2976 3322 1
Size: 12 346 449
Fragments arranged by size:
1012 751 467 449 346 335 246 86
43 23 12
BtgI C'CryG_G
Cuts at: 419 419
Size: 3770
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1720 1901 2064 1720
Size: 181 163 3426
Fragments arranged by size:
3426 181 163
BtsI GCAGTG_nn'
Cuts at: 1013 1013
Size: 3770
DraI TTT'AAA
Cuts at: 314 769 314
Size: 455 3315
DraIII CAC_nnn'GTG
Cuts at: 619 619
Size: 3770
DrdI GACnn_nn'nnGTC
Cuts at: 1476 2334 1476
Size: 858 2912
EaeI y'GGCC_r
Cuts at: 1355 1529 1920 1947 3292 1355
Size: 174 391 27 1345 1833
Fragments arranged by size:
1833 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1355 1355
Size: 3770
EarI CTCTTCn'nnn_
Cuts at: 124 1793 2003 124
Size: 1669 210 1891
Fragments arranged by size:
1891 1669 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 134 1978 2288 2434 3580 134
Size: 1844 310 146 1146 324
Fragments arranged by size:
1844 1146 324 310 146
EcoICRI GAG'CTC
Cuts at: 782 782
Size: 3770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 746 1595 2027 2774 3042 746
Size: 849 432 747 268 1474
Fragments arranged by size:
1474 849 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 271 909 271
Size: 638 3132
EcoRI G'AATT_C
Cuts at: 774 774
Size: 3770
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1789 1789
Size: 3770
FspI TGC'GCA
Cuts at: 1551 1551
Size: 3770
HaeII r_GCGC'y
Cuts at: 1204 1452 2474 3453 1204
Size: 248 1022 979 1521
Fragments arranged by size:
1521 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 255 287 652 684 1612 1642 1644 1674 255
Size: 32 365 32 928 30 2 30 2351
Fragments arranged by size:
2351 928 365 32 32 30 30 2
HincII GTy'rAC
Cuts at: 809 809
Size: 3770
KasI G'GCGC_C
Cuts at: 1200 1448 1200
Size: 248 3522
KpnI G_GTAC'C
Cuts at: 790 790
Size: 3770
MluI A'CGCG_T
Cuts at: 3203 3203
Size: 3770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 587 2441 2625 587
Size: 1854 184 1732
Fragments arranged by size:
1854 1732 184
MscI TGG'CCA
Cuts at: 1531 1531
Size: 3770
MslI CAynn'nnrTG
Cuts at: 1759 1886 1759
Size: 127 3643
MspA1I CmG'CkG
Cuts at: 342 881 1195 1555 2568 2813 342
Size: 539 314 360 1013 245 1299
Fragments arranged by size:
1299 1013 539 360 314 245
NaeI GCC'GGC
Cuts at: 1951 1951
Size: 3770
NarI GG'CG_CC
Cuts at: 1201 1449 1201
Size: 248 3522
NgoMIV G'CCGG_C
Cuts at: 1949 1949
Size: 3770
NspI r_CATG'y
Cuts at: 823 1323 2230 823
Size: 500 907 2363
Fragments arranged by size:
2363 907 500
PciI A'CATG_T
Cuts at: 2226 2226
Size: 3770
PmeI GTTT'AAAC
Cuts at: 769 769
Size: 3770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2909 2941 2909
Size: 32 3738
PpuMI rG'GwC_Cy
Cuts at: 271 271
Size: 3770
PstI C_TGCA'G
Cuts at: 817 1502 817
Size: 685 3085
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3770
PvuII CAG'CTG
Cuts at: 1195 1555 1195
Size: 360 3410
RsrII CG'GwC_CG
Cuts at: 1965 1965
Size: 3770
SacI G_AGCT'C
Cuts at: 784 784
Size: 3770
SalI G'TCGA_C
Cuts at: 807 807
Size: 3770
SapI GCTCTTCn'nnn_
Cuts at: 1793 2003 1793
Size: 210 3560
SbfI CC_TGCA'GG
Cuts at: 817 817
Size: 3770
ScaI AGT'ACT
Cuts at: 3287 3287
Size: 3770
SfcI C'TryA_G
Cuts at: 33 813 1498 2491 2682 33
Size: 780 685 993 191 1121
Fragments arranged by size:
1121 993 780 685 191
SfoI GGC'GCC
Cuts at: 1202 1450 1202
Size: 248 3522
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3770
SmaI CCC'GGG
Cuts at: 792 792
Size: 3770
SmlI C'TyrA_G
Cuts at: 925 1840 2332 2594 925
Size: 915 492 262 2101
Fragments arranged by size:
2101 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3770
SphI G_CATG'C
Cuts at: 823 1323 823
Size: 500 3270
StyI C'CwwG_G
Cuts at: 137 904 1258 137
Size: 767 354 2649
Fragments arranged by size:
2649 767 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1353 1486 1945 2154 1353
Size: 133 459 209 2969
Fragments arranged by size:
2969 459 209 133
TatI w'GTAC_w
Cuts at: 3285 3285
Size: 3770
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 713 745 3287 3319 713
Size: 32 2542 32 1164
Fragments arranged by size:
2542 1164 32 32
Tth111I GACn'n_nGTC
Cuts at: 1567 1567
Size: 3770
XbaI T'CTAG_A
Cuts at: 801 801
Size: 3770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1265 1265
Size: 3770
XmaI C'CCGG_G
Cuts at: 790 790
Size: 3770
XmnI GAAnn'nnTTC
Cuts at: 692 692
Size: 3770
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI