Restriction map for
pF1KB6981
(Circular) (Six-base) MAPSORT of: pF1KB6981.seq Check: 9412 from: 1 to: 3767
pF1KB6981 3767 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 476 805 476
Size: 329 3438
Acc65I G'GTAC_C
Cuts at: 783 783
Size: 3767
AclI AA'CG_TT
Cuts at: 3607 3607
Size: 3767
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1592 2024 2771 3039 1592
Size: 432 747 268 2320
Fragments arranged by size:
2320 747 432 268
AfeI AGC'GCT
Cuts at: 361 361
Size: 3767
AflIII A'CryG_T
Cuts at: 1119 2223 3200 1119
Size: 1104 977 1686
Fragments arranged by size:
1686 1104 977
AgeI A'CCGG_T
Cuts at: 2155 2155
Size: 3767
AlwNI CAG_nnn'CTG
Cuts at: 417 1072 2639 417
Size: 655 1567 1545
Fragments arranged by size:
1567 1545 655
ApaLI G'TGCA_C
Cuts at: 2537 2537
Size: 3767
ApoI r'AATT_y
Cuts at: 218 771 3120 3376 218
Size: 553 2349 256 609
Fragments arranged by size:
2349 609 553 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3767
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3767
AvaI C'yCGr_G
Cuts at: 453 787 453
Size: 334 3433
BamHI G'GATC_C
Cuts at: 792 792
Size: 3767
BanI G'GyrC_C
Cuts at: 397 783 1197 1445 1480 397
Size: 386 414 248 35 2684
Fragments arranged by size:
2684 414 386 248 35
BanII G_rGCy'C
Cuts at: 781 1811 781
Size: 1030 2737
BbeI G_GCGC'C
Cuts at: 1201 1449 1201
Size: 248 3519
BbvCI CC'TCA_GC
Cuts at: 153 153
Size: 3767
BciVI GTATCCnnnnn_n'
Cuts at: 1665 2426 3767 1665
Size: 761 1341 1665
Fragments arranged by size:
1665 1341 761
BclI T'GATC_A
Cuts at: 1287 1287
Size: 3767
BglI GCCn_nnn'nGGC
Cuts at: 293 293
Size: 3767
BglII A'GATC_T
Cuts at: 1282 3319 1282
Size: 2037 1730
BlpI GC'TnA_GC
Cuts at: 879 879
Size: 3767
Bme1580I G_kGCm'C
Cuts at: 298 400 1392 1485 2541 298
Size: 102 992 93 1056 1524
Fragments arranged by size:
1524 1056 992 102 93
BmgBI CAC'GTC
Cuts at: 474 474
Size: 3767
BmrI ACTGGGnnnn_n'
Cuts at: 979 1060 1395 3539 979
Size: 81 335 2144 1207
Fragments arranged by size:
2144 1207 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 198 714 2134 198
Size: 516 1420 1831
Fragments arranged by size:
1831 1420 516
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 367
Size: 3767
Bpu10I CC'TnA_GC
Cuts at: 153 153
Size: 3767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 1822 2314 2612 943
Size: 879 492 298 2098
Fragments arranged by size:
2098 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 181 244 256 3471 181
Size: 63 12 3215 477
Fragments arranged by size:
3215 477 63 12
BsaAI yAC'GTr
Cuts at: 1120 1120
Size: 3767
BsaBI GATnn'nnATC
Cuts at: 6 1281 3318 6
Size: 1275 2037 455
Fragments arranged by size:
2037 1275 455
BsaHI Gr'CG_yC
Cuts at: 746 1198 1446 2148 3644 746
Size: 452 248 702 1496 869
Fragments arranged by size:
1496 869 702 452 248
BsaWI w'CCGG_w
Cuts at: 956 1477 2155 2429 2576 2987 956
Size: 521 678 274 147 411 1736
Fragments arranged by size:
1736 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 316 364 316
Size: 48 3719
BseYI C'CCAG_C
Cuts at: 1192 1950 2527 3023 1192
Size: 758 577 496 1936
Fragments arranged by size:
1936 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 668 723 668
Size: 55 3712
BsiEI CG_ry'CG
Cuts at: 104 1309 1355 2563 104
Size: 1205 46 1208 1308
Fragments arranged by size:
1308 1208 1205 46
BsiHKAI G_wGCw'C
Cuts at: 509 605 781 1559 1749 2541 509
Size: 96 176 778 190 792 1735
Fragments arranged by size:
1735 792 778 190 176 96
BsmI GAATG_Cn'
Cuts at: 699 699
Size: 3767
Bsp1286I G_dGCh'C
Cuts at: 298 400 509 605 781 1392 1485 1559 1749
Size: 102 109 96 176 611 93 74 190
Cuts at: 1749 1811 2541 298
Size: 62 730 1524
Fragments arranged by size:
1524 730 611 190 176 109 102 96
93 74 62
BspEI T'CCGG_A
Cuts at: 2987 2987
Size: 3767
BspHI T'CATG_A
Cuts at: 590 2943 590
Size: 2353 1414
BspMI ACCTGCnnnn'nnnn_
Cuts at: 416 817 1031 1333 1714 416
Size: 401 214 302 381 2469
Fragments arranged by size:
2469 401 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2059 2113 3597 3762 50
Size: 2009 54 1484 165 55
Fragments arranged by size:
2009 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1679 1679
Size: 3767
BsrFI r'CCGG_y
Cuts at: 1765 1946 2155 1765
Size: 181 209 3377
Fragments arranged by size:
3377 209 181
BssSI C'ACGA_G
Cuts at: 628 2038 2396 628
Size: 1410 358 1999
Fragments arranged by size:
1999 1410 358
BstAPI GCAn_nnn'nTGC
Cuts at: 233 1144 233
Size: 911 2856
BstBI TT'CG_AA
Cuts at: 494 2128 494
Size: 1634 2133
BstYI r'GATC_y
Cuts at: 1 314 414 530 792 1259 1282 1617 1863
Size: 313 100 116 262 467 23 335 246
Cuts at: 1863 2875 2961 2973 3319 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 313 262 246
116 100 86 23 12
BtgI C'CryG_G
Cuts at: 107 671 107
Size: 564 3203
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1717 1898 2061 1717
Size: 181 163 3423
Fragments arranged by size:
3423 181 163
BtsI GCAGTG_nn'
Cuts at: 1010 1010
Size: 3767
DraI TTT'AAA
Cuts at: 766 766
Size: 3767
DraIII CAC_nnn'GTG
Cuts at: 275 275
Size: 3767
DrdI GACnn_nn'nnGTC
Cuts at: 1473 2331 1473
Size: 858 2909
EaeI y'GGCC_r
Cuts at: 710 755 1352 1526 1917 1944 3289 710
Size: 45 597 174 391 27 1345 1188
Fragments arranged by size:
1345 1188 597 391 174 45 27
EagI C'GGCC_G
Cuts at: 1352 1352
Size: 3767
EarI CTCTTCn'nnn_
Cuts at: 493 1790 2000 493
Size: 1297 210 2260
Fragments arranged by size:
2260 1297 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 274 1975 2285 2431 3577 274
Size: 1701 310 146 1146 464
Fragments arranged by size:
1701 1146 464 310 146
EcoICRI GAG'CTC
Cuts at: 779 779
Size: 3767
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 1592 2024 2771 3039 367
Size: 1225 432 747 268 1095
Fragments arranged by size:
1225 1095 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 906 906
Size: 3767
EcoRI G'AATT_C
Cuts at: 771 771
Size: 3767
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1786 1786
Size: 3767
FspI TGC'GCA
Cuts at: 1548 1548
Size: 3767
HaeII r_GCGC'y
Cuts at: 243 363 1201 1449 2471 3450 243
Size: 120 838 248 1022 979 560
Fragments arranged by size:
1022 979 838 560 248 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 556 588 1609 1639 1641 1671 556
Size: 32 1021 30 2 30 2652
Fragments arranged by size:
2652 1021 32 30 30 2
HincII GTy'rAC
Cuts at: 617 806 617
Size: 189 3578
KasI G'GCGC_C
Cuts at: 1197 1445 1197
Size: 248 3519
KpnI G_GTAC'C
Cuts at: 787 787
Size: 3767
MluI A'CGCG_T
Cuts at: 3200 3200
Size: 3767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2438 2622 2438
Size: 184 3583
MscI TGG'CCA
Cuts at: 712 1528 712
Size: 816 2951
MslI CAynn'nnrTG
Cuts at: 610 1756 1883 610
Size: 1146 127 2494
Fragments arranged by size:
2494 1146 127
MspA1I CmG'CkG
Cuts at: 345 878 1192 1552 2565 2810 345
Size: 533 314 360 1013 245 1302
Fragments arranged by size:
1302 1013 533 360 314 245
NaeI GCC'GGC
Cuts at: 1948 1948
Size: 3767
NarI GG'CG_CC
Cuts at: 1198 1446 1198
Size: 248 3519
NcoI C'CATG_G
Cuts at: 107 107
Size: 3767
NdeI CA'TA_TG
Cuts at: 622 622
Size: 3767
NgoMIV G'CCGG_C
Cuts at: 1946 1946
Size: 3767
NspI r_CATG'y
Cuts at: 820 1320 2227 820
Size: 500 907 2360
Fragments arranged by size:
2360 907 500
PciI A'CATG_T
Cuts at: 2223 2223
Size: 3767
PmeI GTTT'AAAC
Cuts at: 766 766
Size: 3767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2906 2938 2906
Size: 32 3735
PstI C_TGCA'G
Cuts at: 413 814 1499 413
Size: 401 685 2681
Fragments arranged by size:
2681 685 401
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3767
PvuII CAG'CTG
Cuts at: 345 1192 1552 345
Size: 847 360 2560
Fragments arranged by size:
2560 847 360
RsrII CG'GwC_CG
Cuts at: 1962 1962
Size: 3767
SacI G_AGCT'C
Cuts at: 781 781
Size: 3767
SalI G'TCGA_C
Cuts at: 804 804
Size: 3767
SapI GCTCTTCn'nnn_
Cuts at: 493 1790 2000 493
Size: 1297 210 2260
Fragments arranged by size:
2260 1297 210
SbfI CC_TGCA'GG
Cuts at: 413 814 413
Size: 401 3366
ScaI AGT'ACT
Cuts at: 3284 3284
Size: 3767
SfcI C'TryA_G
Cuts at: 33 409 810 1495 2488 2679 33
Size: 376 401 685 993 191 1121
Fragments arranged by size:
1121 993 685 401 376 191
SfoI GGC'GCC
Cuts at: 1199 1447 1199
Size: 248 3519
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3767
SmaI CCC'GGG
Cuts at: 789 789
Size: 3767
SmlI C'TyrA_G
Cuts at: 922 1837 2329 2591 922
Size: 915 492 262 2098
Fragments arranged by size:
2098 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3767
SphI G_CATG'C
Cuts at: 820 1320 820
Size: 500 3267
StyI C'CwwG_G
Cuts at: 107 901 1255 107
Size: 794 354 2619
Fragments arranged by size:
2619 794 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 205 1350 1483 1942 2151 205
Size: 1145 133 459 209 1821
Fragments arranged by size:
1821 1145 459 209 133
TatI w'GTAC_w
Cuts at: 3282 3282
Size: 3767
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3284 3316 3284
Size: 32 3735
Tth111I GACn'n_nGTC
Cuts at: 1564 1564
Size: 3767
XbaI T'CTAG_A
Cuts at: 798 798
Size: 3767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 564 627 1262 564
Size: 63 635 3069
Fragments arranged by size:
3069 635 63
XmaI C'CCGG_G
Cuts at: 787 787
Size: 3767
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BmtI BsaXI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI