Restriction map for
pF1KB6978
(Circular) (Six-base) MAPSORT of: pF1KB6978.seq Check: 7160 from: 1 to: 3848
pF1KB6978 3848 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 886 886
Size: 3848
Acc65I G'GTAC_C
Cuts at: 864 864
Size: 3848
AclI AA'CG_TT
Cuts at: 3688 3688
Size: 3848
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 1673 2105 2852 3120 766
Size: 907 432 747 268 1494
Fragments arranged by size:
1494 907 747 432 268
AflIII A'CryG_T
Cuts at: 670 1200 2304 3281 670
Size: 530 1104 977 1237
Fragments arranged by size:
1237 1104 977 530
AgeI A'CCGG_T
Cuts at: 2236 2236
Size: 3848
AlwNI CAG_nnn'CTG
Cuts at: 371 1153 2720 371
Size: 782 1567 1499
Fragments arranged by size:
1567 1499 782
ApaLI G'TGCA_C
Cuts at: 2618 2618
Size: 3848
ApoI r'AATT_y
Cuts at: 388 603 723 769 852 3201 3457 388
Size: 215 120 46 83 2349 256 779
Fragments arranged by size:
2349 779 256 215 120 83 46
AseI AT'TA_AT
Cuts at: 20 20
Size: 3848
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
AvaI C'yCGr_G
Cuts at: 868 868
Size: 3848
BamHI G'GATC_C
Cuts at: 873 873
Size: 3848
BanI G'GyrC_C
Cuts at: 864 1278 1526 1561 864
Size: 414 248 35 3151
Fragments arranged by size:
3151 414 248 35
BanII G_rGCy'C
Cuts at: 862 1892 862
Size: 1030 2818
BbeI G_GCGC'C
Cuts at: 1282 1530 1282
Size: 248 3600
BciVI GTATCCnnnnn_n'
Cuts at: 1746 2507 3848 1746
Size: 761 1341 1746
Fragments arranged by size:
1746 1341 761
BclI T'GATC_A
Cuts at: 1368 1368
Size: 3848
BglII A'GATC_T
Cuts at: 473 1363 3400 473
Size: 890 2037 921
Fragments arranged by size:
2037 921 890
BlpI GC'TnA_GC
Cuts at: 960 960
Size: 3848
Bme1580I G_kGCm'C
Cuts at: 1473 1566 2622 1473
Size: 93 1056 2699
Fragments arranged by size:
2699 1056 93
BmgBI CAC'GTC
Cuts at: 134 134
Size: 3848
BmrI ACTGGGnnnn_n'
Cuts at: 1060 1141 1476 3620 1060
Size: 81 335 2144 1288
Fragments arranged by size:
2144 1288 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 138 2215 138
Size: 2077 1771
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 1024 1903 2395 2693 464
Size: 560 879 492 298 1619
Fragments arranged by size:
1619 879 560 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3552 3552
Size: 3848
BsaAI yAC'GTr
Cuts at: 1201 1201
Size: 3848
BsaBI GATnn'nnATC
Cuts at: 6 1362 3399 6
Size: 1356 2037 455
Fragments arranged by size:
2037 1356 455
BsaHI Gr'CG_yC
Cuts at: 1279 1527 2229 3725 1279
Size: 248 702 1496 1402
Fragments arranged by size:
1496 1402 702 248
BsaWI w'CCGG_w
Cuts at: 1037 1558 2236 2510 2657 3068 1037
Size: 521 678 274 147 411 1817
Fragments arranged by size:
1817 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 758 788 758
Size: 30 3818
BseYI C'CCAG_C
Cuts at: 1273 2031 2608 3104 1273
Size: 758 577 496 2017
Fragments arranged by size:
2017 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1390 1436 2644 104
Size: 1286 46 1208 1308
Fragments arranged by size:
1308 1286 1208 46
BsiHKAI G_wGCw'C
Cuts at: 182 264 862 1640 1830 2622 182
Size: 82 598 778 190 792 1408
Fragments arranged by size:
1408 792 778 598 190 82
Bsp1286I G_dGCh'C
Cuts at: 182 264 862 1473 1566 1640 1830 1892 2622
Size: 82 598 611 93 74 190 62 730
Cuts at: 2622 182
Size: 1408
Fragments arranged by size:
1408 730 611 598 190 93 82 74
62
BspEI T'CCGG_A
Cuts at: 3068 3068
Size: 3848
BspHI T'CATG_A
Cuts at: 3024 3024
Size: 3848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 898 1112 1414 1795 898
Size: 214 302 381 2951
Fragments arranged by size:
2951 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2140 2194 3678 3843 50
Size: 2090 54 1484 165 55
Fragments arranged by size:
2090 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 251 1760 251
Size: 1509 2339
BsrFI r'CCGG_y
Cuts at: 1846 2027 2236 1846
Size: 181 209 3458
Fragments arranged by size:
3458 209 181
BssSI C'ACGA_G
Cuts at: 2119 2477 2119
Size: 358 3490
BstAPI GCAn_nnn'nTGC
Cuts at: 1225 1225
Size: 3848
BstBI TT'CG_AA
Cuts at: 2209 2209
Size: 3848
BstYI r'GATC_y
Cuts at: 1 473 702 873 1340 1363 1698 1944 2956
Size: 472 229 171 467 23 335 246 1012
Cuts at: 2956 3042 3054 3400 1
Size: 86 12 346 449
Fragments arranged by size:
1012 472 467 449 346 335 246 229
171 86 23 12
BtgI C'CryG_G
Cuts at: 107 200 107
Size: 93 3755
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1798 1979 2142 1798
Size: 181 163 3504
Fragments arranged by size:
3504 181 163
BtsI GCAGTG_nn'
Cuts at: 1091 1091
Size: 3848
DraI TTT'AAA
Cuts at: 847 847
Size: 3848
DrdI GACnn_nn'nnGTC
Cuts at: 1554 2412 1554
Size: 858 2990
EaeI y'GGCC_r
Cuts at: 110 128 1433 1607 1998 2025 3370 110
Size: 18 1305 174 391 27 1345 588
Fragments arranged by size:
1345 1305 588 391 174 27 18
EagI C'GGCC_G
Cuts at: 1433 1433
Size: 3848
EarI CTCTTCn'nnn_
Cuts at: 291 695 1871 2081 291
Size: 404 1176 210 2058
Fragments arranged by size:
2058 1176 404 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2056 2366 2512 3658 2056
Size: 310 146 1146 2246
Fragments arranged by size:
2246 1146 310 146
EcoICRI GAG'CTC
Cuts at: 860 860
Size: 3848
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 1673 2105 2852 3120 766
Size: 907 432 747 268 1494
Fragments arranged by size:
1494 907 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 117 987 117
Size: 870 2978
EcoRI G'AATT_C
Cuts at: 852 852
Size: 3848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1867 1867
Size: 3848
FspI TGC'GCA
Cuts at: 1629 1629
Size: 3848
HaeII r_GCGC'y
Cuts at: 1282 1530 2552 3531 1282
Size: 248 1022 979 1599
Fragments arranged by size:
1599 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 294 326 1690 1720 1722 1752 294
Size: 32 1364 30 2 30 2390
Fragments arranged by size:
2390 1364 32 30 30 2
HincII GTy'rAC
Cuts at: 887 887
Size: 3848
KasI G'GCGC_C
Cuts at: 1278 1526 1278
Size: 248 3600
KpnI G_GTAC'C
Cuts at: 868 868
Size: 3848
MluI A'CGCG_T
Cuts at: 3281 3281
Size: 3848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2519 2703 2519
Size: 184 3664
MscI TGG'CCA
Cuts at: 1609 1609
Size: 3848
MslI CAynn'nnrTG
Cuts at: 1837 1964 1837
Size: 127 3721
MspA1I CmG'CkG
Cuts at: 959 1273 1633 2646 2891 959
Size: 314 360 1013 245 1916
Fragments arranged by size:
1916 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2029 2029
Size: 3848
NarI GG'CG_CC
Cuts at: 1279 1527 1279
Size: 248 3600
NcoI C'CATG_G
Cuts at: 107 107
Size: 3848
NgoMIV G'CCGG_C
Cuts at: 2027 2027
Size: 3848
NspI r_CATG'y
Cuts at: 311 674 901 1401 2308 311
Size: 363 227 500 907 1851
Fragments arranged by size:
1851 907 500 363 227
PciI A'CATG_T
Cuts at: 670 2304 670
Size: 1634 2214
PmeI GTTT'AAAC
Cuts at: 847 847
Size: 3848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2987 3019 2987
Size: 32 3816
PpuMI rG'GwC_Cy
Cuts at: 117 117
Size: 3848
PstI C_TGCA'G
Cuts at: 367 895 1580 367
Size: 528 685 2635
Fragments arranged by size:
2635 685 528
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3848
PvuII CAG'CTG
Cuts at: 1273 1633 1273
Size: 360 3488
RsrII CG'GwC_CG
Cuts at: 2043 2043
Size: 3848
SacI G_AGCT'C
Cuts at: 862 862
Size: 3848
SalI G'TCGA_C
Cuts at: 885 885
Size: 3848
SapI GCTCTTCn'nnn_
Cuts at: 1871 2081 1871
Size: 210 3638
SbfI CC_TGCA'GG
Cuts at: 895 895
Size: 3848
ScaI AGT'ACT
Cuts at: 3365 3365
Size: 3848
SfcI C'TryA_G
Cuts at: 33 222 363 891 1576 2569 2760 33
Size: 189 141 528 685 993 191 1121
Fragments arranged by size:
1121 993 685 528 191 189 141
SfoI GGC'GCC
Cuts at: 1280 1528 1280
Size: 248 3600
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
SmaI CCC'GGG
Cuts at: 870 870
Size: 3848
SmlI C'TyrA_G
Cuts at: 479 1003 1918 2410 2672 479
Size: 524 915 492 262 1655
Fragments arranged by size:
1655 915 524 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3848
SphI G_CATG'C
Cuts at: 901 1401 901
Size: 500 3348
StyI C'CwwG_G
Cuts at: 107 694 982 1336 107
Size: 587 288 354 2619
Fragments arranged by size:
2619 587 354 288
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1431 1564 2023 2232 1431
Size: 133 459 209 3047
Fragments arranged by size:
3047 459 209 133
TatI w'GTAC_w
Cuts at: 3363 3363
Size: 3848
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 758 790 3365 3397 758
Size: 32 2575 32 1209
Fragments arranged by size:
2575 1209 32 32
Tth111I GACn'n_nGTC
Cuts at: 1645 1645
Size: 3848
XbaI T'CTAG_A
Cuts at: 879 879
Size: 3848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1343 1343
Size: 3848
XmaI C'CCGG_G
Cuts at: 868 868
Size: 3848
XmnI GAAnn'nnTTC
Cuts at: 827 827
Size: 3848
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmtI BpmI Bpu10I BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI