Restriction map for
pF1KB6973
(Circular) (Six-base) MAPSORT of: pF1KB6973.seq Check: 1833 from: 1 to: 3764
pF1KB6973 3764 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 451 451
Size: 3764
AccI GT'mk_AC
Cuts at: 802 802
Size: 3764
Acc65I G'GTAC_C
Cuts at: 780 780
Size: 3764
AclI AA'CG_TT
Cuts at: 3604 3604
Size: 3764
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1589 2021 2768 3036 1589
Size: 432 747 268 2317
Fragments arranged by size:
2317 747 432 268
AflIII A'CryG_T
Cuts at: 1116 2220 3197 1116
Size: 1104 977 1683
Fragments arranged by size:
1683 1104 977
AgeI A'CCGG_T
Cuts at: 2152 2152
Size: 3764
AlwNI CAG_nnn'CTG
Cuts at: 321 1069 2636 321
Size: 748 1567 1449
Fragments arranged by size:
1567 1449 748
ApaI G_GGCC'C
Cuts at: 644 644
Size: 3764
ApaLI G'TGCA_C
Cuts at: 2534 2534
Size: 3764
ApoI r'AATT_y
Cuts at: 294 768 3117 3373 294
Size: 474 2349 256 685
Fragments arranged by size:
2349 685 474 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3764
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
AvaI C'yCGr_G
Cuts at: 520 682 784 520
Size: 162 102 3500
Fragments arranged by size:
3500 162 102
BamHI G'GATC_C
Cuts at: 789 789
Size: 3764
BanI G'GyrC_C
Cuts at: 741 780 1194 1442 1477 741
Size: 39 414 248 35 3028
Fragments arranged by size:
3028 414 248 39 35
BanII G_rGCy'C
Cuts at: 644 778 1808 644
Size: 134 1030 2600
Fragments arranged by size:
2600 1030 134
BbeI G_GCGC'C
Cuts at: 745 1198 1446 745
Size: 453 248 3063
Fragments arranged by size:
3063 453 248
BciVI GTATCCnnnnn_n'
Cuts at: 1662 2423 3764 1662
Size: 761 1341 1662
Fragments arranged by size:
1662 1341 761
BclI T'GATC_A
Cuts at: 194 407 1284 194
Size: 213 877 2674
Fragments arranged by size:
2674 877 213
BglI GCCn_nnn'nGGC
Cuts at: 452 553 452
Size: 101 3663
BglII A'GATC_T
Cuts at: 473 1279 3316 473
Size: 806 2037 921
Fragments arranged by size:
2037 921 806
BlpI GC'TnA_GC
Cuts at: 565 876 565
Size: 311 3453
Bme1580I G_kGCm'C
Cuts at: 644 1389 1482 2538 644
Size: 745 93 1056 1870
Fragments arranged by size:
1870 1056 745 93
BmrI ACTGGGnnnn_n'
Cuts at: 403 976 1057 1392 3536 403
Size: 573 81 335 2144 631
Fragments arranged by size:
2144 631 573 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 3764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 328 514 205
Size: 123 186 3455
Fragments arranged by size:
3455 186 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 940 1819 2311 2609 940
Size: 879 492 298 2095
Fragments arranged by size:
2095 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3468 3468
Size: 3764
BsaAI yAC'GTr
Cuts at: 1117 1117
Size: 3764
BsaBI GATnn'nnATC
Cuts at: 6 1278 3315 6
Size: 1272 2037 455
Fragments arranged by size:
2037 1272 455
BsaHI Gr'CG_yC
Cuts at: 589 742 1195 1443 2145 3641 589
Size: 153 453 248 702 1496 712
Fragments arranged by size:
1496 712 702 453 248 153
BsaWI w'CCGG_w
Cuts at: 953 1474 2152 2426 2573 2984 953
Size: 521 678 274 147 411 1733
Fragments arranged by size:
1733 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 643 643
Size: 3764
BseYI C'CCAG_C
Cuts at: 117 652 1189 1947 2524 3020 117
Size: 535 537 758 577 496 861
Fragments arranged by size:
861 758 577 537 535 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 462 462
Size: 3764
BsiEI CG_ry'CG
Cuts at: 104 731 1306 1352 2560 104
Size: 627 575 46 1208 1308
Fragments arranged by size:
1308 1208 627 575 46
BsiHKAI G_wGCw'C
Cuts at: 778 1556 1746 2538 778
Size: 778 190 792 2004
Fragments arranged by size:
2004 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 580 580
Size: 3764
Bsp1286I G_dGCh'C
Cuts at: 644 778 1389 1482 1556 1746 1808 2538 644
Size: 134 611 93 74 190 62 730 1870
Fragments arranged by size:
1870 730 611 190 134 93 74 62
BspEI T'CCGG_A
Cuts at: 2984 2984
Size: 3764
BspHI T'CATG_A
Cuts at: 2940 2940
Size: 3764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 451 747 814 1028 1330 1711 451
Size: 296 67 214 302 381 2504
Fragments arranged by size:
2504 381 302 296 214 67
BsrBI CCG'CTC
Cuts at: 50 2056 2110 3594 3759 50
Size: 2006 54 1484 165 55
Fragments arranged by size:
2006 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1676 1676
Size: 3764
BsrFI r'CCGG_y
Cuts at: 591 1762 1943 2152 591
Size: 1171 181 209 2203
Fragments arranged by size:
2203 1171 209 181
BsrGI T'GTAC_A
Cuts at: 413 413
Size: 3764
BssSI C'ACGA_G
Cuts at: 2035 2393 2035
Size: 358 3406
BstAPI GCAn_nnn'nTGC
Cuts at: 1141 1141
Size: 3764
BstBI TT'CG_AA
Cuts at: 2125 2125
Size: 3764
BstYI r'GATC_y
Cuts at: 1 473 789 1256 1279 1614 1860 2872 2958
Size: 472 316 467 23 335 246 1012 86
Cuts at: 2958 2970 3316 1
Size: 12 346 449
Fragments arranged by size:
1012 472 467 449 346 335 316 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 176 383 107
Size: 69 207 3488
Fragments arranged by size:
3488 207 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1714 1895 2058 1714
Size: 181 163 3420
Fragments arranged by size:
3420 181 163
BtsI GCAGTG_nn'
Cuts at: 1007 1007
Size: 3764
DraI TTT'AAA
Cuts at: 763 763
Size: 3764
DrdI GACnn_nn'nnGTC
Cuts at: 244 1470 2328 244
Size: 1226 858 1680
Fragments arranged by size:
1680 1226 858
EaeI y'GGCC_r
Cuts at: 545 668 695 728 1349 1523 1914 1941 3286
Size: 123 27 33 621 174 391 27 1345
Cuts at: 3286 545
Size: 1023
Fragments arranged by size:
1345 1023 621 391 174 123 33 27
27
EagI C'GGCC_G
Cuts at: 728 1349 728
Size: 621 3143
EarI CTCTTCn'nnn_
Cuts at: 1787 1997 1787
Size: 210 3554
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 718 1972 2282 2428 3574 718
Size: 1254 310 146 1146 908
Fragments arranged by size:
1254 1146 908 310 146
EcoICRI GAG'CTC
Cuts at: 776 776
Size: 3764
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 328 514 1589 2021 2768 3036 205
Size: 123 186 1075 432 747 268 933
Fragments arranged by size:
1075 933 747 432 268 186 123
EcoO109I rG'GnC_Cy
Cuts at: 554 640 903 554
Size: 86 263 3415
Fragments arranged by size:
3415 263 86
EcoRI G'AATT_C
Cuts at: 768 768
Size: 3764
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1783 1783
Size: 3764
FspI TGC'GCA
Cuts at: 1545 1545
Size: 3764
HaeII r_GCGC'y
Cuts at: 391 528 745 1198 1446 2468 3447 391
Size: 137 217 453 248 1022 979 708
Fragments arranged by size:
1022 979 708 453 248 217 137
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1606 1636 1638 1668 1606
Size: 30 2 30 3702
Fragments arranged by size:
3702 30 30 2
HincII GTy'rAC
Cuts at: 803 803
Size: 3764
KasI G'GCGC_C
Cuts at: 741 1194 1442 741
Size: 453 248 3063
Fragments arranged by size:
3063 453 248
KpnI G_GTAC'C
Cuts at: 784 784
Size: 3764
MluI A'CGCG_T
Cuts at: 3197 3197
Size: 3764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2435 2619 2435
Size: 184 3580
MscI TGG'CCA
Cuts at: 1525 1525
Size: 3764
MslI CAynn'nnrTG
Cuts at: 511 1753 1880 511
Size: 1242 127 2395
Fragments arranged by size:
2395 1242 127
MspA1I CmG'CkG
Cuts at: 273 486 564 875 1189 1549 2562 2807 273
Size: 213 78 311 314 360 1013 245 1230
Fragments arranged by size:
1230 1013 360 314 311 245 213 78
NaeI GCC'GGC
Cuts at: 593 1945 593
Size: 1352 2412
NarI GG'CG_CC
Cuts at: 742 1195 1443 742
Size: 453 248 3063
Fragments arranged by size:
3063 453 248
NcoI C'CATG_G
Cuts at: 107 176 383 107
Size: 69 207 3488
Fragments arranged by size:
3488 207 69
NgoMIV G'CCGG_C
Cuts at: 591 1943 591
Size: 1352 2412
NspI r_CATG'y
Cuts at: 817 1317 2224 817
Size: 500 907 2357
Fragments arranged by size:
2357 907 500
PasI CC'CwG_GG
Cuts at: 154 154
Size: 3764
PciI A'CATG_T
Cuts at: 2220 2220
Size: 3764
PmeI GTTT'AAAC
Cuts at: 763 763
Size: 3764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2903 2935 2903
Size: 32 3732
PspOMI G'GGCC_C
Cuts at: 640 640
Size: 3764
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 421 453 421
Size: 32 3732
PstI C_TGCA'G
Cuts at: 127 331 811 1496 127
Size: 204 480 685 2395
Fragments arranged by size:
2395 685 480 204
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3764
PvuII CAG'CTG
Cuts at: 273 1189 1549 273
Size: 916 360 2488
Fragments arranged by size:
2488 916 360
RsrII CG'GwC_CG
Cuts at: 1959 1959
Size: 3764
SacI G_AGCT'C
Cuts at: 778 778
Size: 3764
SalI G'TCGA_C
Cuts at: 801 801
Size: 3764
SapI GCTCTTCn'nnn_
Cuts at: 1787 1997 1787
Size: 210 3554
SbfI CC_TGCA'GG
Cuts at: 811 811
Size: 3764
ScaI AGT'ACT
Cuts at: 3281 3281
Size: 3764
SexAI A'CCwGG_T
Cuts at: 239 239
Size: 3764
SfcI C'TryA_G
Cuts at: 33 123 327 807 1492 2485 2676 33
Size: 90 204 480 685 993 191 1121
Fragments arranged by size:
1121 993 685 480 204 191 90
SfiI GGCCn_nnn'nGGCC
Cuts at: 553 553
Size: 3764
SfoI GGC'GCC
Cuts at: 743 1196 1444 743
Size: 453 248 3063
Fragments arranged by size:
3063 453 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
SgrAI Cr'CCGG_yG
Cuts at: 591 591
Size: 3764
SmaI CCC'GGG
Cuts at: 684 786 684
Size: 102 3662
SmlI C'TyrA_G
Cuts at: 919 1834 2326 2588 919
Size: 915 492 262 2095
Fragments arranged by size:
2095 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3764
SphI G_CATG'C
Cuts at: 817 1317 817
Size: 500 3264
StyI C'CwwG_G
Cuts at: 107 176 203 383 623 698 898 1252 107
Size: 69 27 180 240 75 200 354 2619
Fragments arranged by size:
2619 354 240 200 180 75 69 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 350 661 1347 1480 1939 2148 350
Size: 311 686 133 459 209 1966
Fragments arranged by size:
1966 686 459 311 209 133
TatI w'GTAC_w
Cuts at: 413 500 3279 413
Size: 87 2779 898
Fragments arranged by size:
2779 898 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3281 3313 3281
Size: 32 3732
Tth111I GACn'n_nGTC
Cuts at: 1561 1561
Size: 3764
XbaI T'CTAG_A
Cuts at: 795 795
Size: 3764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 390 1259 114
Size: 276 869 2619
Fragments arranged by size:
2619 869 276
XmaI C'CCGG_G
Cuts at: 682 784 682
Size: 102 3662
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PmeI PpiI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI
SacII SanDI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI