Restriction map for
pF1KB6967
(Circular) (Six-base) MAPSORT of: pF1KB6967.seq Check: 9353 from: 1 to: 3758
pF1KB6967 3758 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AatII G_ACGT'C
Cuts at: 690 690
Size: 3758
AccI GT'mk_AC
Cuts at: 796 796
Size: 3758
Acc65I G'GTAC_C
Cuts at: 774 774
Size: 3758
AclI AA'CG_TT
Cuts at: 3598 3598
Size: 3758
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1583 2015 2762 3030 1583
Size: 432 747 268 2311
Fragments arranged by size:
2311 747 432 268
AflIII A'CryG_T
Cuts at: 1110 2214 3191 1110
Size: 1104 977 1677
Fragments arranged by size:
1677 1104 977
AgeI A'CCGG_T
Cuts at: 2146 2146
Size: 3758
AlwNI CAG_nnn'CTG
Cuts at: 178 1063 2630 178
Size: 885 1567 1306
Fragments arranged by size:
1567 1306 885
ApaLI G'TGCA_C
Cuts at: 2528 2528
Size: 3758
ApoI r'AATT_y
Cuts at: 491 762 3111 3367 491
Size: 271 2349 256 882
Fragments arranged by size:
2349 882 271 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3758
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3758
AvaI C'yCGr_G
Cuts at: 124 778 124
Size: 654 3104
BamHI G'GATC_C
Cuts at: 783 783
Size: 3758
BanI G'GyrC_C
Cuts at: 148 774 1188 1436 1471 148
Size: 626 414 248 35 2435
Fragments arranged by size:
2435 626 414 248 35
BanII G_rGCy'C
Cuts at: 374 393 772 1802 374
Size: 19 379 1030 2330
Fragments arranged by size:
2330 1030 379 19
BbeI G_GCGC'C
Cuts at: 1192 1440 1192
Size: 248 3510
BbsI GAAGACnn'nnnn_
Cuts at: 202 202
Size: 3758
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3724
BciVI GTATCCnnnnn_n'
Cuts at: 1656 2417 3758 1656
Size: 761 1341 1656
Fragments arranged by size:
1656 1341 761
BclI T'GATC_A
Cuts at: 1278 1278
Size: 3758
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3758
BglII A'GATC_T
Cuts at: 1273 3310 1273
Size: 2037 1721
BlpI GC'TnA_GC
Cuts at: 870 870
Size: 3758
Bme1580I G_kGCm'C
Cuts at: 151 172 1383 1476 2532 151
Size: 21 1211 93 1056 1377
Fragments arranged by size:
1377 1211 1056 93 21
BmrI ACTGGGnnnn_n'
Cuts at: 248 970 1051 1386 3530 248
Size: 722 81 335 2144 476
Fragments arranged by size:
2144 722 476 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 575 2125 575
Size: 1550 2208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 934 1813 2305 2603 934
Size: 879 492 298 2089
Fragments arranged by size:
2089 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3462 3462
Size: 3758
BsaAI yAC'GTr
Cuts at: 1111 1111
Size: 3758
BsaBI GATnn'nnATC
Cuts at: 6 579 671 1272 3309 6
Size: 573 92 601 2037 455
Fragments arranged by size:
2037 601 573 455 92
BsaHI Gr'CG_yC
Cuts at: 687 1189 1437 2139 3635 687
Size: 502 248 702 1496 810
Fragments arranged by size:
1496 810 702 502 248
BsaWI w'CCGG_w
Cuts at: 332 947 1468 2146 2420 2567 2978 332
Size: 615 521 678 274 147 411 1112
Fragments arranged by size:
1112 678 615 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 587 587
Size: 3758
BseYI C'CCAG_C
Cuts at: 178 1183 1941 2518 3014 178
Size: 1005 758 577 496 922
Fragments arranged by size:
1005 922 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 752 752
Size: 3758
BsiEI CG_ry'CG
Cuts at: 104 1300 1346 2554 104
Size: 1196 46 1208 1308
Fragments arranged by size:
1308 1208 1196 46
BsiHKAI G_wGCw'C
Cuts at: 374 393 772 1550 1740 2532 374
Size: 19 379 778 190 792 1600
Fragments arranged by size:
1600 792 778 379 190 19
BsmBI CGTCTCn'nnnn_
Cuts at: 678 732 678
Size: 54 3704
Bsp1286I G_dGCh'C
Cuts at: 151 172 374 393 772 1383 1476 1550 1740
Size: 21 202 19 379 611 93 74 190
Cuts at: 1740 1802 2532 151
Size: 62 730 1377
Fragments arranged by size:
1377 730 611 379 202 190 93 74
62 21 19
BspEI T'CCGG_A
Cuts at: 332 2978 332
Size: 2646 1112
BspHI T'CATG_A
Cuts at: 2934 2934
Size: 3758
BspMI ACCTGCnnnn'nnnn_
Cuts at: 106 486 808 1022 1324 1705 106
Size: 380 322 214 302 381 2159
Fragments arranged by size:
2159 381 380 322 302 214
BsrBI CCG'CTC
Cuts at: 50 2050 2104 3588 3753 50
Size: 2000 54 1484 165 55
Fragments arranged by size:
2000 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1670 1670
Size: 3758
BsrFI r'CCGG_y
Cuts at: 1756 1937 2146 1756
Size: 181 209 3368
Fragments arranged by size:
3368 209 181
BssSI C'ACGA_G
Cuts at: 636 2029 2387 636
Size: 1393 358 2007
Fragments arranged by size:
2007 1393 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1135 1135
Size: 3758
BstBI TT'CG_AA
Cuts at: 2119 2119
Size: 3758
BstXI CCAn_nnnn'nTGG
Cuts at: 289 564 289
Size: 275 3483
BstYI r'GATC_y
Cuts at: 1 783 1250 1273 1608 1854 2866 2952 2964
Size: 782 467 23 335 246 1012 86 12
Cuts at: 2964 3310 1
Size: 346 449
Fragments arranged by size:
1012 782 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1708 1889 2052 1708
Size: 181 163 3414
Fragments arranged by size:
3414 181 163
BtsI GCAGTG_nn'
Cuts at: 1001 1001
Size: 3758
DraI TTT'AAA
Cuts at: 617 757 617
Size: 140 3618
DrdI GACnn_nn'nnGTC
Cuts at: 1464 2322 1464
Size: 858 2900
EaeI y'GGCC_r
Cuts at: 537 1343 1517 1908 1935 3280 537
Size: 806 174 391 27 1345 1015
Fragments arranged by size:
1345 1015 806 391 174 27
EagI C'GGCC_G
Cuts at: 1343 1343
Size: 3758
EarI CTCTTCn'nnn_
Cuts at: 509 693 1781 1991 509
Size: 184 1088 210 2276
Fragments arranged by size:
2276 1088 210 184
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1966 2276 2422 3568 1966
Size: 310 146 1146 2156
Fragments arranged by size:
2156 1146 310 146
EcoICRI GAG'CTC
Cuts at: 372 391 770 372
Size: 19 379 3360
Fragments arranged by size:
3360 379 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1583 2015 2762 3030 1583
Size: 432 747 268 2311
Fragments arranged by size:
2311 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 135 162 897 135
Size: 27 735 2996
Fragments arranged by size:
2996 735 27
EcoRI G'AATT_C
Cuts at: 762 762
Size: 3758
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1777 1777
Size: 3758
FspI TGC'GCA
Cuts at: 1539 1539
Size: 3758
HaeII r_GCGC'y
Cuts at: 1192 1440 2462 3441 1192
Size: 248 1022 979 1509
Fragments arranged by size:
1509 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1600 1630 1632 1662 1600
Size: 30 2 30 3696
Fragments arranged by size:
3696 30 30 2
HincII GTy'rAC
Cuts at: 121 797 121
Size: 676 3082
KasI G'GCGC_C
Cuts at: 1188 1436 1188
Size: 248 3510
KpnI G_GTAC'C
Cuts at: 778 778
Size: 3758
MluI A'CGCG_T
Cuts at: 3191 3191
Size: 3758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2429 2613 2429
Size: 184 3574
MscI TGG'CCA
Cuts at: 539 1519 539
Size: 980 2778
MslI CAynn'nnrTG
Cuts at: 344 1747 1874 344
Size: 1403 127 2228
Fragments arranged by size:
2228 1403 127
MspA1I CmG'CkG
Cuts at: 202 399 405 508 869 1183 1543 2556 2801
Size: 197 6 103 361 314 360 1013 245
Cuts at: 2801 202
Size: 1159
Fragments arranged by size:
1159 1013 361 360 314 245 197 103
6
NaeI GCC'GGC
Cuts at: 1939 1939
Size: 3758
NarI GG'CG_CC
Cuts at: 1189 1437 1189
Size: 248 3510
NgoMIV G'CCGG_C
Cuts at: 1937 1937
Size: 3758
NspI r_CATG'y
Cuts at: 811 1311 2218 811
Size: 500 907 2351
Fragments arranged by size:
2351 907 500
PciI A'CATG_T
Cuts at: 2214 2214
Size: 3758
PflMI CCAn_nnn'nTGG
Cuts at: 178 178
Size: 3758
PfoI T'CCnGG_A
Cuts at: 679 679
Size: 3758
PmeI GTTT'AAAC
Cuts at: 757 757
Size: 3758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2897 2929 2897
Size: 32 3726
PpuMI rG'GwC_Cy
Cuts at: 135 162 135
Size: 27 3731
PshAI GACnn'nnGTC
Cuts at: 300 300
Size: 3758
PspXI vC'TCGA_Gb
Cuts at: 124 124
Size: 3758
PstI C_TGCA'G
Cuts at: 398 404 432 805 1490 398
Size: 6 28 373 685 2666
Fragments arranged by size:
2666 685 373 28 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3758
PvuII CAG'CTG
Cuts at: 202 399 508 1183 1543 202
Size: 197 109 675 360 2417
Fragments arranged by size:
2417 675 360 197 109
RsrII CG'GwC_CG
Cuts at: 1953 1953
Size: 3758
SacI G_AGCT'C
Cuts at: 374 393 772 374
Size: 19 379 3360
Fragments arranged by size:
3360 379 19
SalI G'TCGA_C
Cuts at: 795 795
Size: 3758
SanDI GG'GwC_CC
Cuts at: 135 135
Size: 3758
SapI GCTCTTCn'nnn_
Cuts at: 693 1781 1991 693
Size: 1088 210 2460
Fragments arranged by size:
2460 1088 210
SbfI CC_TGCA'GG
Cuts at: 805 805
Size: 3758
ScaI AGT'ACT
Cuts at: 3275 3275
Size: 3758
SfcI C'TryA_G
Cuts at: 33 394 400 428 801 1486 2479 2670 33
Size: 361 6 28 373 685 993 191 1121
Fragments arranged by size:
1121 993 685 373 361 191 28 6
SfoI GGC'GCC
Cuts at: 1190 1438 1190
Size: 248 3510
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3758
SmaI CCC'GGG
Cuts at: 780 780
Size: 3758
SmlI C'TyrA_G
Cuts at: 124 913 1828 2320 2582 124
Size: 789 915 492 262 1300
Fragments arranged by size:
1300 915 789 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3758
SphI G_CATG'C
Cuts at: 811 1311 811
Size: 500 3258
StyI C'CwwG_G
Cuts at: 892 1246 892
Size: 354 3404
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 166 170 1341 1474 1933 2142 166
Size: 4 1171 133 459 209 1782
Fragments arranged by size:
1782 1171 459 209 133 4
TatI w'GTAC_w
Cuts at: 3273 3273
Size: 3758
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3275 3307 3275
Size: 32 3726
Tth111I GACn'n_nGTC
Cuts at: 1555 1555
Size: 3758
XbaI T'CTAG_A
Cuts at: 789 789
Size: 3758
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 286 1253 286
Size: 967 2791
XhoI C'TCGA_G
Cuts at: 124 124
Size: 3758
XmaI C'CCGG_G
Cuts at: 778 778
Size: 3758
XmnI GAAnn'nnTTC
Cuts at: 492 492
Size: 3758
ZraI GAC'GTC
Cuts at: 688 688
Size: 3758
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI BpmI
Bpu10I BsaXI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PsiI PspOMI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI