Restriction map for
pF1KB6966
(Circular) (Six-base) MAPSORT of: pF1KB6966.seq Check: 6244 from: 1 to: 3836
pF1KB6966 3836 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 874 874
Size: 3836
Acc65I G'GTAC_C
Cuts at: 383 852 383
Size: 469 3367
AclI AA'CG_TT
Cuts at: 3676 3676
Size: 3836
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 341 538 599 1661 2093 2840 3108 134
Size: 207 197 61 1062 432 747 268 862
Fragments arranged by size:
1062 862 747 432 268 207 197 61
AflIII A'CryG_T
Cuts at: 1188 2292 3269 1188
Size: 1104 977 1755
Fragments arranged by size:
1755 1104 977
AgeI A'CCGG_T
Cuts at: 2224 2224
Size: 3836
AleI CACnn'nnGTG
Cuts at: 322 322
Size: 3836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 340 387 664 340
Size: 47 277 3512
Fragments arranged by size:
3512 277 47
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 397 429 397
Size: 32 3804
AlwNI CAG_nnn'CTG
Cuts at: 195 279 1141 2708 195
Size: 84 862 1567 1323
Fragments arranged by size:
1567 1323 862 84
ApaLI G'TGCA_C
Cuts at: 325 2606 325
Size: 2281 1555
ApoI r'AATT_y
Cuts at: 352 445 465 760 840 3189 3445 352
Size: 93 20 295 80 2349 256 743
Fragments arranged by size:
2349 743 295 256 93 80 20
AseI AT'TA_AT
Cuts at: 20 20
Size: 3836
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
AvaI C'yCGr_G
Cuts at: 706 752 856 706
Size: 46 104 3686
Fragments arranged by size:
3686 104 46
BamHI G'GATC_C
Cuts at: 861 861
Size: 3836
BanI G'GyrC_C
Cuts at: 383 852 1266 1514 1549 383
Size: 469 414 248 35 2670
Fragments arranged by size:
2670 469 414 248 35
BanII G_rGCy'C
Cuts at: 626 800 850 1880 626
Size: 174 50 1030 2582
Fragments arranged by size:
2582 1030 174 50
BbeI G_GCGC'C
Cuts at: 1270 1518 1270
Size: 248 3588
BciVI GTATCCnnnnn_n'
Cuts at: 1734 2495 3836 1734
Size: 761 1341 1734
Fragments arranged by size:
1734 1341 761
BclI T'GATC_A
Cuts at: 1356 1356
Size: 3836
BglII A'GATC_T
Cuts at: 1351 3388 1351
Size: 2037 1799
BlpI GC'TnA_GC
Cuts at: 948 948
Size: 3836
Bme1580I G_kGCm'C
Cuts at: 286 329 376 1461 1554 2610 286
Size: 43 47 1085 93 1056 1512
Fragments arranged by size:
1512 1085 1056 93 47 43
BmrI ACTGGGnnnn_n'
Cuts at: 1048 1129 1464 3608 1048
Size: 81 335 2144 1276
Fragments arranged by size:
2144 1276 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 3836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 514 379
Size: 135 3701
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 756 1012 1891 2383 2681 199
Size: 557 256 879 492 298 1354
Fragments arranged by size:
1354 879 557 492 298 256
BsaI GGTCTCn'nnnn_
Cuts at: 3540 3540
Size: 3836
BsaAI yAC'GTr
Cuts at: 1189 1189
Size: 3836
BsaBI GATnn'nnATC
Cuts at: 6 1350 3387 6
Size: 1344 2037 455
Fragments arranged by size:
2037 1344 455
BsaHI Gr'CG_yC
Cuts at: 1267 1515 2217 3713 1267
Size: 248 702 1496 1390
Fragments arranged by size:
1496 1390 702 248
BsaWI w'CCGG_w
Cuts at: 1025 1546 2224 2498 2645 3056 1025
Size: 521 678 274 147 411 1805
Fragments arranged by size:
1805 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 802 802
Size: 3836
BseYI C'CCAG_C
Cuts at: 424 474 1261 2019 2596 3092 424
Size: 50 787 758 577 496 1168
Fragments arranged by size:
1168 787 758 577 496 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 292
Size: 3836
BsiEI CG_ry'CG
Cuts at: 104 1378 1424 2632 104
Size: 1274 46 1208 1308
Fragments arranged by size:
1308 1274 1208 46
BsiHKAI G_wGCw'C
Cuts at: 329 626 800 850 1628 1818 2610 329
Size: 297 174 50 778 190 792 1555
Fragments arranged by size:
1555 792 778 297 190 174 50
Bsp1286I G_dGCh'C
Cuts at: 286 329 376 626 800 850 1461 1554 1628
Size: 43 47 250 174 50 611 93 74
Cuts at: 1628 1818 1880 2610 286
Size: 190 62 730 1512
Fragments arranged by size:
1512 730 611 250 190 174 93 74
62 50 47 43
BspEI T'CCGG_A
Cuts at: 3056 3056
Size: 3836
BspHI T'CATG_A
Cuts at: 3012 3012
Size: 3836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 117 886 1100 1402 1783 117
Size: 769 214 302 381 2170
Fragments arranged by size:
2170 769 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2128 2182 3666 3831 50
Size: 2078 54 1484 165 55
Fragments arranged by size:
2078 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 747 1748 747
Size: 1001 2835
BsrFI r'CCGG_y
Cuts at: 1834 2015 2224 1834
Size: 181 209 3446
Fragments arranged by size:
3446 209 181
BssSI C'ACGA_G
Cuts at: 2107 2465 2107
Size: 358 3478
BstAPI GCAn_nnn'nTGC
Cuts at: 1213 1213
Size: 3836
BstBI TT'CG_AA
Cuts at: 2197 2197
Size: 3836
BstYI r'GATC_y
Cuts at: 1 861 1328 1351 1686 1932 2944 3030 3042
Size: 860 467 23 335 246 1012 86 12
Cuts at: 3042 3388 1
Size: 346 449
Fragments arranged by size:
1012 860 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3836
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1786 1967 2130 1786
Size: 181 163 3492
Fragments arranged by size:
3492 181 163
BtsI GCAGTG_nn'
Cuts at: 159 371 1079 159
Size: 212 708 2916
Fragments arranged by size:
2916 708 212
DraI TTT'AAA
Cuts at: 835 835
Size: 3836
DrdI GACnn_nn'nnGTC
Cuts at: 1542 2400 1542
Size: 858 2978
EaeI y'GGCC_r
Cuts at: 1421 1595 1986 2013 3358 1421
Size: 174 391 27 1345 1899
Fragments arranged by size:
1899 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1421 1421
Size: 3836
EarI CTCTTCn'nnn_
Cuts at: 1859 2069 1859
Size: 210 3626
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 273 2044 2354 2500 3646 273
Size: 1771 310 146 1146 463
Fragments arranged by size:
1771 1146 463 310 146
EcoICRI GAG'CTC
Cuts at: 624 798 848 624
Size: 174 50 3612
Fragments arranged by size:
3612 174 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 341 379 514 538 599 1661 2093 2840
Size: 207 38 135 24 61 1062 432 747
Cuts at: 2840 3108 134
Size: 268 862
Fragments arranged by size:
1062 862 747 432 268 207 135 61
38 24
EcoO109I rG'GnC_Cy
Cuts at: 822 975 822
Size: 153 3683
EcoRI G'AATT_C
Cuts at: 465 760 840 465
Size: 295 80 3461
Fragments arranged by size:
3461 295 80
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1855 1855
Size: 3836
FspI TGC'GCA
Cuts at: 1617 1617
Size: 3836
HaeII r_GCGC'y
Cuts at: 1270 1518 2540 3519 1270
Size: 248 1022 979 1587
Fragments arranged by size:
1587 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 686 718 1678 1708 1710 1740 686
Size: 32 960 30 2 30 2782
Fragments arranged by size:
2782 960 32 30 30 2
HincII GTy'rAC
Cuts at: 246 875 246
Size: 629 3207
KasI G'GCGC_C
Cuts at: 1266 1514 1266
Size: 248 3588
KpnI G_GTAC'C
Cuts at: 387 856 387
Size: 469 3367
MluI A'CGCG_T
Cuts at: 3269 3269
Size: 3836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 440 2507 2691 440
Size: 2067 184 1585
Fragments arranged by size:
2067 1585 184
MscI TGG'CCA
Cuts at: 1597 1597
Size: 3836
MslI CAynn'nnrTG
Cuts at: 322 1825 1952 322
Size: 1503 127 2206
Fragments arranged by size:
2206 1503 127
MspA1I CmG'CkG
Cuts at: 424 947 1261 1621 2634 2879 424
Size: 523 314 360 1013 245 1381
Fragments arranged by size:
1381 1013 523 360 314 245
NaeI GCC'GGC
Cuts at: 2017 2017
Size: 3836
NarI GG'CG_CC
Cuts at: 1267 1515 1267
Size: 248 3588
NcoI C'CATG_G
Cuts at: 107 107
Size: 3836
NgoMIV G'CCGG_C
Cuts at: 2015 2015
Size: 3836
NspI r_CATG'y
Cuts at: 889 1389 2296 889
Size: 500 907 2429
Fragments arranged by size:
2429 907 500
PasI CC'CwG_GG
Cuts at: 286 286
Size: 3836
PciI A'CATG_T
Cuts at: 2292 2292
Size: 3836
PmeI GTTT'AAAC
Cuts at: 835 835
Size: 3836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2975 3007 2975
Size: 32 3804
PstI C_TGCA'G
Cuts at: 883 1568 883
Size: 685 3151
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3836
PvuII CAG'CTG
Cuts at: 424 1261 1621 424
Size: 837 360 2639
Fragments arranged by size:
2639 837 360
RsrII CG'GwC_CG
Cuts at: 260 2031 260
Size: 1771 2065
SacI G_AGCT'C
Cuts at: 626 800 850 626
Size: 174 50 3612
Fragments arranged by size:
3612 174 50
SalI G'TCGA_C
Cuts at: 873 873
Size: 3836
SapI GCTCTTCn'nnn_
Cuts at: 1859 2069 1859
Size: 210 3626
SbfI CC_TGCA'GG
Cuts at: 883 883
Size: 3836
ScaI AGT'ACT
Cuts at: 3353 3353
Size: 3836
SfcI C'TryA_G
Cuts at: 33 879 1564 2557 2748 33
Size: 846 685 993 191 1121
Fragments arranged by size:
1121 993 846 685 191
SfoI GGC'GCC
Cuts at: 1268 1516 1268
Size: 248 3588
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
SmaI CCC'GGG
Cuts at: 754 858 754
Size: 104 3732
SmlI C'TyrA_G
Cuts at: 214 735 991 1906 2398 2660 214
Size: 521 256 915 492 262 1390
Fragments arranged by size:
1390 915 521 492 262 256
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3836
SphI G_CATG'C
Cuts at: 889 1389 889
Size: 500 3336
StuI AGG'CCT
Cuts at: 712 712
Size: 3836
StyI C'CwwG_G
Cuts at: 107 970 1324 107
Size: 863 354 2619
Fragments arranged by size:
2619 863 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 277 1419 1552 2011 2220 277
Size: 1142 133 459 209 1893
Fragments arranged by size:
1893 1142 459 209 133
TatI w'GTAC_w
Cuts at: 666 3351 666
Size: 2685 1151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3353 3385 3353
Size: 32 3804
Tth111I GACn'n_nGTC
Cuts at: 1633 1633
Size: 3836
XbaI T'CTAG_A
Cuts at: 867 867
Size: 3836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1331 1331
Size: 3836
XmaI C'CCGG_G
Cuts at: 752 856 752
Size: 104 3732
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI