Restriction map for
pF1KB6963
(Circular) (Six-base) MAPSORT of: pF1KB6963.seq Check: 9907 from: 1 to: 3755
pF1KB6963 3755 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 169 793 169
Size: 624 3131
Acc65I G'GTAC_C
Cuts at: 771 771
Size: 3755
AclI AA'CG_TT
Cuts at: 3595 3595
Size: 3755
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 1580 2012 2759 3027 141
Size: 1439 432 747 268 869
Fragments arranged by size:
1439 869 747 432 268
AflII C'TTAA_G
Cuts at: 609 609
Size: 3755
AflIII A'CryG_T
Cuts at: 1107 2211 3188 1107
Size: 1104 977 1674
Fragments arranged by size:
1674 1104 977
AgeI A'CCGG_T
Cuts at: 2143 2143
Size: 3755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 563 563
Size: 3755
AlwNI CAG_nnn'CTG
Cuts at: 1060 2627 1060
Size: 1567 2188
ApaLI G'TGCA_C
Cuts at: 2525 2525
Size: 3755
ApoI r'AATT_y
Cuts at: 759 3108 3364 759
Size: 2349 256 1150
Fragments arranged by size:
2349 1150 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3755
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3755
AvaI C'yCGr_G
Cuts at: 775 775
Size: 3755
BamHI G'GATC_C
Cuts at: 780 780
Size: 3755
BanI G'GyrC_C
Cuts at: 262 771 1185 1433 1468 262
Size: 509 414 248 35 2549
Fragments arranged by size:
2549 509 414 248 35
BanII G_rGCy'C
Cuts at: 165 769 1799 165
Size: 604 1030 2121
Fragments arranged by size:
2121 1030 604
BbeI G_GCGC'C
Cuts at: 1189 1437 1189
Size: 248 3507
BbsI GAAGACnn'nnnn_
Cuts at: 147 746 147
Size: 599 3156
BciVI GTATCCnnnnn_n'
Cuts at: 1653 2414 3755 1653
Size: 761 1341 1653
Fragments arranged by size:
1653 1341 761
BclI T'GATC_A
Cuts at: 1275 1275
Size: 3755
BglI GCCn_nnn'nGGC
Cuts at: 455 455
Size: 3755
BglII A'GATC_T
Cuts at: 1270 3307 1270
Size: 2037 1718
BlpI GC'TnA_GC
Cuts at: 308 867 308
Size: 559 3196
Bme1580I G_kGCm'C
Cuts at: 1380 1473 2529 1380
Size: 93 1056 2606
Fragments arranged by size:
2606 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 450 967 1048 1383 3527 450
Size: 517 81 335 2144 678
Fragments arranged by size:
2144 678 517 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 3755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 173
Size: 3755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 384 931 1810 2302 2600 384
Size: 547 879 492 298 1539
Fragments arranged by size:
1539 879 547 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3459 3459
Size: 3755
BsaAI yAC'GTr
Cuts at: 1108 1108
Size: 3755
BsaBI GATnn'nnATC
Cuts at: 6 1269 3306 6
Size: 1263 2037 455
Fragments arranged by size:
2037 1263 455
BsaHI Gr'CG_yC
Cuts at: 1186 1434 2136 3632 1186
Size: 248 702 1496 1309
Fragments arranged by size:
1496 1309 702 248
BsaWI w'CCGG_w
Cuts at: 944 1465 2143 2417 2564 2975 944
Size: 521 678 274 147 411 1724
Fragments arranged by size:
1724 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1180 1938 2515 3011 1180
Size: 758 577 496 1924
Fragments arranged by size:
1924 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1297 1343 2551 104
Size: 1193 46 1208 1308
Fragments arranged by size:
1308 1208 1193 46
BsiHKAI G_wGCw'C
Cuts at: 769 1547 1737 2529 769
Size: 778 190 792 1995
Fragments arranged by size:
1995 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 165 769 1380 1473 1547 1737 1799 2529 165
Size: 604 611 93 74 190 62 730 1391
Fragments arranged by size:
1391 730 611 604 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2975 2975
Size: 3755
BspHI T'CATG_A
Cuts at: 2931 2931
Size: 3755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 805 1019 1321 1702 805
Size: 214 302 381 2858
Fragments arranged by size:
2858 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2047 2101 3585 3750 50
Size: 1997 54 1484 165 55
Fragments arranged by size:
1997 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 334 720 1667 334
Size: 386 947 2422
Fragments arranged by size:
2422 947 386
BsrFI r'CCGG_y
Cuts at: 1753 1934 2143 1753
Size: 181 209 3365
Fragments arranged by size:
3365 209 181
BssSI C'ACGA_G
Cuts at: 2026 2384 2026
Size: 358 3397
BstAPI GCAn_nnn'nTGC
Cuts at: 595 1132 595
Size: 537 3218
BstBI TT'CG_AA
Cuts at: 702 2116 702
Size: 1414 2341
BstYI r'GATC_y
Cuts at: 1 780 1247 1270 1605 1851 2863 2949 2961
Size: 779 467 23 335 246 1012 86 12
Cuts at: 2961 3307 1
Size: 346 449
Fragments arranged by size:
1012 779 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 170 170
Size: 3755
BtgI C'CryG_G
Cuts at: 450 450
Size: 3755
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1705 1886 2049 1705
Size: 181 163 3411
Fragments arranged by size:
3411 181 163
BtsI GCAGTG_nn'
Cuts at: 428 998 428
Size: 570 3185
DraI TTT'AAA
Cuts at: 754 754
Size: 3755
DrdI GACnn_nn'nnGTC
Cuts at: 1461 2319 1461
Size: 858 2897
EaeI y'GGCC_r
Cuts at: 1340 1514 1905 1932 3277 1340
Size: 174 391 27 1345 1818
Fragments arranged by size:
1818 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1340 1340
Size: 3755
EarI CTCTTCn'nnn_
Cuts at: 1778 1988 1778
Size: 210 3545
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1963 2273 2419 3565 1963
Size: 310 146 1146 2153
Fragments arranged by size:
2153 1146 310 146
EcoICRI GAG'CTC
Cuts at: 767 767
Size: 3755
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 173 1580 2012 2759 3027 141
Size: 32 1407 432 747 268 869
Fragments arranged by size:
1407 869 747 432 268 32
EcoO109I rG'GnC_Cy
Cuts at: 894 894
Size: 3755
EcoRI G'AATT_C
Cuts at: 759 759
Size: 3755
EcoRV GAT'ATC
Cuts at: 570 570
Size: 3755
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 651 1774 651
Size: 1123 2632
FspI TGC'GCA
Cuts at: 1536 1536
Size: 3755
HaeII r_GCGC'y
Cuts at: 1189 1437 2459 3438 1189
Size: 248 1022 979 1506
Fragments arranged by size:
1506 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 727 759 1597 1627 1629 1659 727
Size: 32 838 30 2 30 2823
Fragments arranged by size:
2823 838 32 30 30 2
HincII GTy'rAC
Cuts at: 794 794
Size: 3755
KasI G'GCGC_C
Cuts at: 1185 1433 1185
Size: 248 3507
KpnI G_GTAC'C
Cuts at: 775 775
Size: 3755
MfeI C'AATT_G
Cuts at: 249 249
Size: 3755
MluI A'CGCG_T
Cuts at: 3188 3188
Size: 3755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2426 2610 2426
Size: 184 3571
MscI TGG'CCA
Cuts at: 1516 1516
Size: 3755
MslI CAynn'nnrTG
Cuts at: 1744 1871 1744
Size: 127 3628
MspA1I CmG'CkG
Cuts at: 866 1180 1540 2553 2798 866
Size: 314 360 1013 245 1823
Fragments arranged by size:
1823 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1936 1936
Size: 3755
NarI GG'CG_CC
Cuts at: 1186 1434 1186
Size: 248 3507
NgoMIV G'CCGG_C
Cuts at: 1934 1934
Size: 3755
NspI r_CATG'y
Cuts at: 544 808 1308 2215 544
Size: 264 500 907 2084
Fragments arranged by size:
2084 907 500 264
PciI A'CATG_T
Cuts at: 2211 2211
Size: 3755
PflMI CCAn_nnn'nTGG
Cuts at: 254 254
Size: 3755
PmeI GTTT'AAAC
Cuts at: 754 754
Size: 3755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2894 2926 2894
Size: 32 3723
PstI C_TGCA'G
Cuts at: 802 1487 802
Size: 685 3070
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3755
PvuII CAG'CTG
Cuts at: 1180 1540 1180
Size: 360 3395
RsrII CG'GwC_CG
Cuts at: 1950 1950
Size: 3755
SacI G_AGCT'C
Cuts at: 769 769
Size: 3755
SalI G'TCGA_C
Cuts at: 792 792
Size: 3755
SapI GCTCTTCn'nnn_
Cuts at: 1778 1988 1778
Size: 210 3545
SbfI CC_TGCA'GG
Cuts at: 802 802
Size: 3755
ScaI AGT'ACT
Cuts at: 3272 3272
Size: 3755
SfcI C'TryA_G
Cuts at: 33 798 1483 2476 2667 33
Size: 765 685 993 191 1121
Fragments arranged by size:
1121 993 765 685 191
SfoI GGC'GCC
Cuts at: 1187 1435 1187
Size: 248 3507
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3755
SmaI CCC'GGG
Cuts at: 777 777
Size: 3755
SmlI C'TyrA_G
Cuts at: 363 609 910 1825 2317 2579 363
Size: 246 301 915 492 262 1539
Fragments arranged by size:
1539 915 492 301 262 246
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3755
SphI G_CATG'C
Cuts at: 544 808 1308 544
Size: 264 500 2991
Fragments arranged by size:
2991 500 264
SspI AAT'ATT
Cuts at: 498 498
Size: 3755
StyI C'CwwG_G
Cuts at: 889 1243 889
Size: 354 3401
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 138 388 1338 1471 1930 2139 138
Size: 250 950 133 459 209 1754
Fragments arranged by size:
1754 950 459 250 209 133
TatI w'GTAC_w
Cuts at: 3270 3270
Size: 3755
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3272 3304 3272
Size: 32 3723
Tth111I GACn'n_nGTC
Cuts at: 1552 1552
Size: 3755
XbaI T'CTAG_A
Cuts at: 786 786
Size: 3755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1250 1250
Size: 3755
XmaI C'CCGG_G
Cuts at: 775 775
Size: 3755
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI