Restriction map for
pF1KB6955
(Circular) (Six-base) MAPSORT of: pF1KB6955.seq Check: 1898 from: 1 to: 3749
pF1KB6955 3749 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 321 787 321
Size: 466 3283
Acc65I G'GTAC_C
Cuts at: 765 765
Size: 3749
AclI AA'CG_TT
Cuts at: 3589 3589
Size: 3749
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1574 2006 2753 3021 1574
Size: 432 747 268 2302
Fragments arranged by size:
2302 747 432 268
AflIII A'CryG_T
Cuts at: 1101 2205 3182 1101
Size: 1104 977 1668
Fragments arranged by size:
1668 1104 977
AgeI A'CCGG_T
Cuts at: 2137 2137
Size: 3749
AlwNI CAG_nnn'CTG
Cuts at: 464 1054 2621 464
Size: 590 1567 1592
Fragments arranged by size:
1592 1567 590
ApaLI G'TGCA_C
Cuts at: 2519 2519
Size: 3749
ApoI r'AATT_y
Cuts at: 262 753 3102 3358 262
Size: 491 2349 256 653
Fragments arranged by size:
2349 653 491 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3749
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
AvaI C'yCGr_G
Cuts at: 769 769
Size: 3749
BamHI G'GATC_C
Cuts at: 634 774 634
Size: 140 3609
BanI G'GyrC_C
Cuts at: 765 1179 1427 1462 765
Size: 414 248 35 3052
Fragments arranged by size:
3052 414 248 35
BanII G_rGCy'C
Cuts at: 763 1793 763
Size: 1030 2719
BbeI G_GCGC'C
Cuts at: 1183 1431 1183
Size: 248 3501
BciVI GTATCCnnnnn_n'
Cuts at: 1647 2408 3749 1647
Size: 761 1341 1647
Fragments arranged by size:
1647 1341 761
BclI T'GATC_A
Cuts at: 1269 1269
Size: 3749
BglII A'GATC_T
Cuts at: 544 730 1264 3301 544
Size: 186 534 2037 992
Fragments arranged by size:
2037 992 534 186
BlpI GC'TnA_GC
Cuts at: 861 861
Size: 3749
Bme1580I G_kGCm'C
Cuts at: 1374 1467 2523 1374
Size: 93 1056 2600
Fragments arranged by size:
2600 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 113 961 1042 1377 3521 113
Size: 848 81 335 2144 341
Fragments arranged by size:
2144 848 341 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 162 2116 162
Size: 1954 1795
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 1804 2296 2594 925
Size: 879 492 298 2080
Fragments arranged by size:
2080 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 617 3453 617
Size: 2836 913
BsaAI yAC'GTr
Cuts at: 1102 1102
Size: 3749
BsaBI GATnn'nnATC
Cuts at: 6 735 1263 3300 6
Size: 729 528 2037 455
Fragments arranged by size:
2037 729 528 455
BsaHI Gr'CG_yC
Cuts at: 1180 1428 2130 3626 1180
Size: 248 702 1496 1303
Fragments arranged by size:
1496 1303 702 248
BsaWI w'CCGG_w
Cuts at: 938 1459 2137 2411 2558 2969 938
Size: 521 678 274 147 411 1718
Fragments arranged by size:
1718 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 534 564 534
Size: 30 3719
BseYI C'CCAG_C
Cuts at: 1174 1932 2509 3005 1174
Size: 758 577 496 1918
Fragments arranged by size:
1918 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 327 683 327
Size: 356 3393
BsiEI CG_ry'CG
Cuts at: 104 1291 1337 2545 104
Size: 1187 46 1208 1308
Fragments arranged by size:
1308 1208 1187 46
BsiHKAI G_wGCw'C
Cuts at: 249 763 1541 1731 2523 249
Size: 514 778 190 792 1475
Fragments arranged by size:
1475 792 778 514 190
Bsp1286I G_dGCh'C
Cuts at: 249 763 1374 1467 1541 1731 1793 2523 249
Size: 514 611 93 74 190 62 730 1475
Fragments arranged by size:
1475 730 611 514 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2969 2969
Size: 3749
BspHI T'CATG_A
Cuts at: 675 2925 675
Size: 2250 1499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 499 799 1013 1315 1696 499
Size: 300 214 302 381 2552
Fragments arranged by size:
2552 381 302 300 214
BsrBI CCG'CTC
Cuts at: 50 2041 2095 3579 3744 50
Size: 1991 54 1484 165 55
Fragments arranged by size:
1991 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 406 1661 406
Size: 1255 2494
BsrFI r'CCGG_y
Cuts at: 1747 1928 2137 1747
Size: 181 209 3359
Fragments arranged by size:
3359 209 181
BssSI C'ACGA_G
Cuts at: 212 2020 2378 212
Size: 1808 358 1583
Fragments arranged by size:
1808 1583 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1126 1126
Size: 3749
BstBI TT'CG_AA
Cuts at: 2110 2110
Size: 3749
BstXI CCAn_nnnn'nTGG
Cuts at: 403 403
Size: 3749
BstYI r'GATC_y
Cuts at: 1 387 544 634 730 774 1241 1264 1599
Size: 386 157 90 96 44 467 23 335
Cuts at: 1599 1845 2857 2943 2955 3301 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 386 346 335 246 157
96 90 86 44 23 12
BstZ17I GTA'TAC
Cuts at: 322 322
Size: 3749
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1699 1880 2043 1699
Size: 181 163 3405
Fragments arranged by size:
3405 181 163
BtsI GCAGTG_nn'
Cuts at: 522 992 522
Size: 470 3279
DraI TTT'AAA
Cuts at: 267 748 267
Size: 481 3268
DrdI GACnn_nn'nnGTC
Cuts at: 1455 2313 1455
Size: 858 2891
EaeI y'GGCC_r
Cuts at: 1334 1508 1899 1926 3271 1334
Size: 174 391 27 1345 1812
Fragments arranged by size:
1812 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1334 1334
Size: 3749
EarI CTCTTCn'nnn_
Cuts at: 152 254 473 1772 1982 152
Size: 102 219 1299 210 1919
Fragments arranged by size:
1919 1299 219 210 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1957 2267 2413 3559 1957
Size: 310 146 1146 2147
Fragments arranged by size:
2147 1146 310 146
EcoICRI GAG'CTC
Cuts at: 761 761
Size: 3749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1574 2006 2753 3021 1574
Size: 432 747 268 2302
Fragments arranged by size:
2302 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 888 888
Size: 3749
EcoRI G'AATT_C
Cuts at: 753 753
Size: 3749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1768 1768
Size: 3749
FspI TGC'GCA
Cuts at: 1530 1530
Size: 3749
HaeII r_GCGC'y
Cuts at: 1183 1431 2453 3432 1183
Size: 248 1022 979 1500
Fragments arranged by size:
1500 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 534 566 577 609 709 741 1591 1621 1623
Size: 32 11 32 100 32 850 30 2
Cuts at: 1623 1653 534
Size: 30 2630
Fragments arranged by size:
2630 850 100 32 32 32 30 30
11 2
HincII GTy'rAC
Cuts at: 788 788
Size: 3749
KasI G'GCGC_C
Cuts at: 1179 1427 1179
Size: 248 3501
KpnI G_GTAC'C
Cuts at: 769 769
Size: 3749
MluI A'CGCG_T
Cuts at: 3182 3182
Size: 3749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2420 2604 2420
Size: 184 3565
MscI TGG'CCA
Cuts at: 1510 1510
Size: 3749
MslI CAynn'nnrTG
Cuts at: 527 1738 1865 527
Size: 1211 127 2411
Fragments arranged by size:
2411 1211 127
MspA1I CmG'CkG
Cuts at: 461 860 1174 1534 2547 2792 461
Size: 399 314 360 1013 245 1418
Fragments arranged by size:
1418 1013 399 360 314 245
NaeI GCC'GGC
Cuts at: 1930 1930
Size: 3749
NarI GG'CG_CC
Cuts at: 1180 1428 1180
Size: 248 3501
NgoMIV G'CCGG_C
Cuts at: 1928 1928
Size: 3749
NspI r_CATG'y
Cuts at: 802 1302 2209 802
Size: 500 907 2342
Fragments arranged by size:
2342 907 500
PciI A'CATG_T
Cuts at: 2205 2205
Size: 3749
PmeI GTTT'AAAC
Cuts at: 748 748
Size: 3749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2888 2920 2888
Size: 32 3717
PstI C_TGCA'G
Cuts at: 796 1481 796
Size: 685 3064
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3749
PvuII CAG'CTG
Cuts at: 461 1174 1534 461
Size: 713 360 2676
Fragments arranged by size:
2676 713 360
RsrII CG'GwC_CG
Cuts at: 1944 1944
Size: 3749
SacI G_AGCT'C
Cuts at: 763 763
Size: 3749
SalI G'TCGA_C
Cuts at: 786 786
Size: 3749
SapI GCTCTTCn'nnn_
Cuts at: 152 254 473 1772 1982 152
Size: 102 219 1299 210 1919
Fragments arranged by size:
1919 1299 219 210 102
SbfI CC_TGCA'GG
Cuts at: 796 796
Size: 3749
ScaI AGT'ACT
Cuts at: 3266 3266
Size: 3749
SfcI C'TryA_G
Cuts at: 33 792 1477 2470 2661 33
Size: 759 685 993 191 1121
Fragments arranged by size:
1121 993 759 685 191
SfoI GGC'GCC
Cuts at: 1181 1429 1181
Size: 248 3501
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
SmaI CCC'GGG
Cuts at: 771 771
Size: 3749
SmlI C'TyrA_G
Cuts at: 904 1819 2311 2573 904
Size: 915 492 262 2080
Fragments arranged by size:
2080 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3749
SphI G_CATG'C
Cuts at: 802 1302 802
Size: 500 3249
StyI C'CwwG_G
Cuts at: 883 1237 883
Size: 354 3395
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 430 1332 1465 1924 2133 430
Size: 902 133 459 209 2046
Fragments arranged by size:
2046 902 459 209 133
TatI w'GTAC_w
Cuts at: 3264 3264
Size: 3749
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3266 3298 3266
Size: 32 3717
Tth111I GACn'n_nGTC
Cuts at: 1546 1546
Size: 3749
XbaI T'CTAG_A
Cuts at: 780 780
Size: 3749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1244 1244
Size: 3749
XmaI C'CCGG_G
Cuts at: 769 769
Size: 3749
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BseRI BsiWI BsmI
BsmBI BsrGI BssHII BstEII Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI