Restriction map for
pF1KB6951
(Circular) (Six-base) MAPSORT of: pF1KB6951.seq Check: 9730 from: 1 to: 3749
pF1KB6951 3749 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 787 787
Size: 3749
Acc65I G'GTAC_C
Cuts at: 765 765
Size: 3749
AclI AA'CG_TT
Cuts at: 3589 3589
Size: 3749
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 487 1574 2006 2753 3021 181
Size: 306 1087 432 747 268 909
Fragments arranged by size:
1087 909 747 432 306 268
AfeI AGC'GCT
Cuts at: 492 492
Size: 3749
AflIII A'CryG_T
Cuts at: 602 642 1101 2205 3182 602
Size: 40 459 1104 977 1169
Fragments arranged by size:
1169 1104 977 459 40
AgeI A'CCGG_T
Cuts at: 2137 2137
Size: 3749
AlwNI CAG_nnn'CTG
Cuts at: 1054 2621 1054
Size: 1567 2182
ApaLI G'TGCA_C
Cuts at: 2519 2519
Size: 3749
ApoI r'AATT_y
Cuts at: 753 3102 3358 753
Size: 2349 256 1144
Fragments arranged by size:
2349 1144 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3749
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
AvaI C'yCGr_G
Cuts at: 769 769
Size: 3749
BamHI G'GATC_C
Cuts at: 572 774 572
Size: 202 3547
BanI G'GyrC_C
Cuts at: 765 1179 1427 1462 765
Size: 414 248 35 3052
Fragments arranged by size:
3052 414 248 35
BanII G_rGCy'C
Cuts at: 763 1793 763
Size: 1030 2719
BbeI G_GCGC'C
Cuts at: 1183 1431 1183
Size: 248 3501
BciVI GTATCCnnnnn_n'
Cuts at: 1647 2408 3749 1647
Size: 761 1341 1647
Fragments arranged by size:
1647 1341 761
BclI T'GATC_A
Cuts at: 356 1269 356
Size: 913 2836
BfrBI ATG'CAT
Cuts at: 134 134
Size: 3749
BglII A'GATC_T
Cuts at: 411 629 1264 3301 411
Size: 218 635 2037 859
Fragments arranged by size:
2037 859 635 218
BlpI GC'TnA_GC
Cuts at: 861 861
Size: 3749
Bme1580I G_kGCm'C
Cuts at: 1374 1467 2523 1374
Size: 93 1056 2600
Fragments arranged by size:
2600 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 961 1042 1377 3521 961
Size: 81 335 2144 1189
Fragments arranged by size:
2144 1189 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 3749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 760 760
Size: 3749
Bpu10I CC'TnA_GC
Cuts at: 314 314
Size: 3749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 925 1804 2296 2594 505
Size: 420 879 492 298 1660
Fragments arranged by size:
1660 879 492 420 298
BsaI GGTCTCn'nnnn_
Cuts at: 3453 3453
Size: 3749
BsaAI yAC'GTr
Cuts at: 605 1102 605
Size: 497 3252
BsaBI GATnn'nnATC
Cuts at: 6 1263 3300 6
Size: 1257 2037 455
Fragments arranged by size:
2037 1257 455
BsaHI Gr'CG_yC
Cuts at: 253 1180 1428 2130 3626 253
Size: 927 248 702 1496 376
Fragments arranged by size:
1496 927 702 376 248
BsaWI w'CCGG_w
Cuts at: 258 938 1459 2137 2411 2558 2969 258
Size: 680 521 678 274 147 411 1038
Fragments arranged by size:
1038 680 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 445 475 445
Size: 30 3719
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 385 466 385
Size: 81 3668
BseYI C'CCAG_C
Cuts at: 1174 1932 2509 3005 1174
Size: 758 577 496 1918
Fragments arranged by size:
1918 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 386 211
Size: 175 3574
BsiEI CG_ry'CG
Cuts at: 104 1291 1337 2545 104
Size: 1187 46 1208 1308
Fragments arranged by size:
1308 1208 1187 46
BsiHKAI G_wGCw'C
Cuts at: 763 1541 1731 2523 763
Size: 778 190 792 1989
Fragments arranged by size:
1989 792 778 190
BsmI GAATG_Cn'
Cuts at: 333 333
Size: 3749
BsmBI CGTCTCn'nnnn_
Cuts at: 244 244
Size: 3749
Bsp1286I G_dGCh'C
Cuts at: 763 1374 1467 1541 1731 1793 2523 763
Size: 611 93 74 190 62 730 1989
Fragments arranged by size:
1989 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2969 2969
Size: 3749
BspHI T'CATG_A
Cuts at: 2925 2925
Size: 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 799 1013 1315 1696 799
Size: 214 302 381 2852
Fragments arranged by size:
2852 381 302 214
BsrBI CCG'CTC
Cuts at: 50 270 2041 2095 3579 3744 50
Size: 220 1771 54 1484 165 55
Fragments arranged by size:
1771 1484 220 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1661 1661
Size: 3749
BsrFI r'CCGG_y
Cuts at: 1747 1928 2137 1747
Size: 181 209 3359
Fragments arranged by size:
3359 209 181
BssSI C'ACGA_G
Cuts at: 2020 2378 2020
Size: 358 3391
BstAPI GCAn_nnn'nTGC
Cuts at: 1126 1126
Size: 3749
BstBI TT'CG_AA
Cuts at: 2110 2110
Size: 3749
BstXI CCAn_nnnn'nTGG
Cuts at: 559 559
Size: 3749
BstYI r'GATC_y
Cuts at: 1 326 411 572 629 774 1241 1264 1599
Size: 325 85 161 57 145 467 23 335
Cuts at: 1599 1845 2857 2943 2955 3301 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 325 246 161
145 86 85 57 23 12
BtgI C'CryG_G
Cuts at: 107 243 107
Size: 136 3613
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1699 1880 2043 1699
Size: 181 163 3405
Fragments arranged by size:
3405 181 163
BtsI GCAGTG_nn'
Cuts at: 992 992
Size: 3749
DraI TTT'AAA
Cuts at: 748 748
Size: 3749
DrdI GACnn_nn'nnGTC
Cuts at: 1455 2313 1455
Size: 858 2891
EaeI y'GGCC_r
Cuts at: 1334 1508 1899 1926 3271 1334
Size: 174 391 27 1345 1812
Fragments arranged by size:
1812 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1334 1334
Size: 3749
EarI CTCTTCn'nnn_
Cuts at: 1772 1982 1772
Size: 210 3539
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1957 2267 2413 3559 1957
Size: 310 146 1146 2147
Fragments arranged by size:
2147 1146 310 146
EcoICRI GAG'CTC
Cuts at: 761 761
Size: 3749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 487 760 1574 2006 2753 3021 181
Size: 306 273 814 432 747 268 909
Fragments arranged by size:
909 814 747 432 306 273 268
EcoO109I rG'GnC_Cy
Cuts at: 201 234 888 201
Size: 33 654 3062
Fragments arranged by size:
3062 654 33
EcoRI G'AATT_C
Cuts at: 753 753
Size: 3749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1768 1768
Size: 3749
FspI TGC'GCA
Cuts at: 1530 1530
Size: 3749
HaeII r_GCGC'y
Cuts at: 494 1183 1431 2453 3432 494
Size: 689 248 1022 979 811
Fragments arranged by size:
1022 979 811 689 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 223 255 344 376 526 558 1591 1621 1623
Size: 32 89 32 150 32 1033 30 2
Cuts at: 1623 1653 223
Size: 30 2319
Fragments arranged by size:
2319 1033 150 89 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 788 788
Size: 3749
KasI G'GCGC_C
Cuts at: 1179 1427 1179
Size: 248 3501
KpnI G_GTAC'C
Cuts at: 769 769
Size: 3749
MluI A'CGCG_T
Cuts at: 3182 3182
Size: 3749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2420 2604 2420
Size: 184 3565
MscI TGG'CCA
Cuts at: 1510 1510
Size: 3749
MslI CAynn'nnrTG
Cuts at: 187 557 1738 1865 187
Size: 370 1181 127 2071
Fragments arranged by size:
2071 1181 370 127
MspA1I CmG'CkG
Cuts at: 738 860 1174 1534 2547 2792 738
Size: 122 314 360 1013 245 1695
Fragments arranged by size:
1695 1013 360 314 245 122
NaeI GCC'GGC
Cuts at: 1930 1930
Size: 3749
NarI GG'CG_CC
Cuts at: 1180 1428 1180
Size: 248 3501
NcoI C'CATG_G
Cuts at: 107 107
Size: 3749
NgoMIV G'CCGG_C
Cuts at: 1928 1928
Size: 3749
NsiI A_TGCA'T
Cuts at: 136 136
Size: 3749
NspI r_CATG'y
Cuts at: 134 646 802 1302 2209 134
Size: 512 156 500 907 1674
Fragments arranged by size:
1674 907 512 500 156
PasI CC'CwG_GG
Cuts at: 728 728
Size: 3749
PciI A'CATG_T
Cuts at: 642 2205 642
Size: 1563 2186
PmeI GTTT'AAAC
Cuts at: 748 748
Size: 3749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2888 2920 2888
Size: 32 3717
PpuMI rG'GwC_Cy
Cuts at: 201 201
Size: 3749
PstI C_TGCA'G
Cuts at: 796 1481 796
Size: 685 3064
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3749
PvuII CAG'CTG
Cuts at: 738 1174 1534 738
Size: 436 360 2953
Fragments arranged by size:
2953 436 360
RsrII CG'GwC_CG
Cuts at: 1944 1944
Size: 3749
SacI G_AGCT'C
Cuts at: 763 763
Size: 3749
SalI G'TCGA_C
Cuts at: 786 786
Size: 3749
SapI GCTCTTCn'nnn_
Cuts at: 1772 1982 1772
Size: 210 3539
SbfI CC_TGCA'GG
Cuts at: 796 796
Size: 3749
ScaI AGT'ACT
Cuts at: 3266 3266
Size: 3749
SfcI C'TryA_G
Cuts at: 33 792 1477 2470 2661 33
Size: 759 685 993 191 1121
Fragments arranged by size:
1121 993 759 685 191
SfoI GGC'GCC
Cuts at: 1181 1429 1181
Size: 248 3501
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
SmaI CCC'GGG
Cuts at: 771 771
Size: 3749
SmlI C'TyrA_G
Cuts at: 520 904 1819 2311 2573 520
Size: 384 915 492 262 1696
Fragments arranged by size:
1696 915 492 384 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3749
SphI G_CATG'C
Cuts at: 802 1302 802
Size: 500 3249
StyI C'CwwG_G
Cuts at: 107 883 1237 107
Size: 776 354 2619
Fragments arranged by size:
2619 776 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1332 1465 1924 2133 1332
Size: 133 459 209 2948
Fragments arranged by size:
2948 459 209 133
TatI w'GTAC_w
Cuts at: 3264 3264
Size: 3749
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 445 477 3266 3298 445
Size: 32 2789 32 896
Fragments arranged by size:
2789 896 32 32
Tth111I GACn'n_nGTC
Cuts at: 1546 1546
Size: 3749
XbaI T'CTAG_A
Cuts at: 780 780
Size: 3749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 735 1244 735
Size: 509 3240
XmaI C'CCGG_G
Cuts at: 769 769
Size: 3749
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI