Restriction map for
pF1KB6950
(Circular) (Six-base) MAPSORT of: pF1KB6950.seq Check: 7548 from: 1 to: 3815
pF1KB6950 3815 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 853 853
Size: 3815
Acc65I G'GTAC_C
Cuts at: 831 831
Size: 3815
AclI AA'CG_TT
Cuts at: 3655 3655
Size: 3815
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 800 1640 2072 2819 3087 800
Size: 840 432 747 268 1528
Fragments arranged by size:
1528 840 747 432 268
AflIII A'CryG_T
Cuts at: 1167 2271 3248 1167
Size: 1104 977 1734
Fragments arranged by size:
1734 1104 977
AgeI A'CCGG_T
Cuts at: 2203 2203
Size: 3815
AlwNI CAG_nnn'CTG
Cuts at: 1120 2687 1120
Size: 1567 2248
ApaI G_GGCC'C
Cuts at: 349 349
Size: 3815
ApaLI G'TGCA_C
Cuts at: 367 2585 367
Size: 2218 1597
ApoI r'AATT_y
Cuts at: 819 3168 3424 819
Size: 2349 256 1210
Fragments arranged by size:
2349 1210 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3815
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3815
AvaI C'yCGr_G
Cuts at: 835 835
Size: 3815
BamHI G'GATC_C
Cuts at: 840 840
Size: 3815
BanI G'GyrC_C
Cuts at: 831 1245 1493 1528 831
Size: 414 248 35 3118
Fragments arranged by size:
3118 414 248 35
BanII G_rGCy'C
Cuts at: 207 349 378 829 1859 207
Size: 142 29 451 1030 2163
Fragments arranged by size:
2163 1030 451 142 29
BbeI G_GCGC'C
Cuts at: 1249 1497 1249
Size: 248 3567
BbsI GAAGACnn'nnnn_
Cuts at: 625 625
Size: 3815
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 358 392 358
Size: 34 3781
BciVI GTATCCnnnnn_n'
Cuts at: 1713 2474 3815 1713
Size: 761 1341 1713
Fragments arranged by size:
1713 1341 761
BclI T'GATC_A
Cuts at: 1335 1335
Size: 3815
BglII A'GATC_T
Cuts at: 648 1330 3367 648
Size: 682 2037 1096
Fragments arranged by size:
2037 1096 682
BlpI GC'TnA_GC
Cuts at: 431 927 431
Size: 496 3319
Bme1580I G_kGCm'C
Cuts at: 349 371 1440 1533 2589 349
Size: 22 1069 93 1056 1575
Fragments arranged by size:
1575 1069 1056 93 22
BmrI ACTGGGnnnn_n'
Cuts at: 1027 1108 1443 3587 1027
Size: 81 335 2144 1255
Fragments arranged by size:
2144 1255 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 788 2182 788
Size: 1394 2421
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 584 218
Size: 366 3449
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 991 1870 2362 2660 649
Size: 342 879 492 298 1804
Fragments arranged by size:
1804 879 492 342 298
BsaI GGTCTCn'nnnn_
Cuts at: 3519 3519
Size: 3815
BsaAI yAC'GTr
Cuts at: 1168 1168
Size: 3815
BsaBI GATnn'nnATC
Cuts at: 6 1329 3366 6
Size: 1323 2037 455
Fragments arranged by size:
2037 1323 455
BsaHI Gr'CG_yC
Cuts at: 1246 1494 2196 3692 1246
Size: 248 702 1496 1369
Fragments arranged by size:
1496 1369 702 248
BsaWI w'CCGG_w
Cuts at: 1004 1525 2203 2477 2624 3035 1004
Size: 521 678 274 147 411 1784
Fragments arranged by size:
1784 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 106 136 106
Size: 30 3785
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 215 215
Size: 3815
BseYI C'CCAG_C
Cuts at: 1240 1998 2575 3071 1240
Size: 758 577 496 1984
Fragments arranged by size:
1984 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1357 1403 2611 104
Size: 1253 46 1208 1308
Fragments arranged by size:
1308 1253 1208 46
BsiHKAI G_wGCw'C
Cuts at: 371 378 829 1607 1797 2589 371
Size: 7 451 778 190 792 1597
Fragments arranged by size:
1597 792 778 451 190 7
Bsp1286I G_dGCh'C
Cuts at: 207 349 371 378 829 1440 1533 1607 1797
Size: 142 22 7 451 611 93 74 190
Cuts at: 1797 1859 2589 207
Size: 62 730 1433
Fragments arranged by size:
1433 730 611 451 190 142 93 74
62 22 7
BspEI T'CCGG_A
Cuts at: 3035 3035
Size: 3815
BspHI T'CATG_A
Cuts at: 2991 2991
Size: 3815
BspMI ACCTGCnnnn'nnnn_
Cuts at: 865 1079 1381 1762 865
Size: 214 302 381 2918
Fragments arranged by size:
2918 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2107 2161 3645 3810 50
Size: 2057 54 1484 165 55
Fragments arranged by size:
2057 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 599 753 1727 599
Size: 154 974 2687
Fragments arranged by size:
2687 974 154
BsrFI r'CCGG_y
Cuts at: 1813 1994 2203 1813
Size: 181 209 3425
Fragments arranged by size:
3425 209 181
BsrGI T'GTAC_A
Cuts at: 291 332 291
Size: 41 3774
BssSI C'ACGA_G
Cuts at: 2086 2444 2086
Size: 358 3457
BstAPI GCAn_nnn'nTGC
Cuts at: 1192 1192
Size: 3815
BstBI TT'CG_AA
Cuts at: 2176 2176
Size: 3815
BstYI r'GATC_y
Cuts at: 1 555 648 840 1307 1330 1665 1911 2923
Size: 554 93 192 467 23 335 246 1012
Cuts at: 2923 3009 3021 3367 1
Size: 86 12 346 449
Fragments arranged by size:
1012 554 467 449 346 335 246 192
93 86 23 12
BtgI C'CryG_G
Cuts at: 107 212 107
Size: 105 3710
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1765 1946 2109 1765
Size: 181 163 3471
Fragments arranged by size:
3471 181 163
BtsI GCAGTG_nn'
Cuts at: 1058 1058
Size: 3815
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 218 253 218
Size: 35 3780
DraI TTT'AAA
Cuts at: 814 814
Size: 3815
DrdI GACnn_nn'nnGTC
Cuts at: 1521 2379 1521
Size: 858 2957
EaeI y'GGCC_r
Cuts at: 1400 1574 1965 1992 3337 1400
Size: 174 391 27 1345 1878
Fragments arranged by size:
1878 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1400 1400
Size: 3815
EarI CTCTTCn'nnn_
Cuts at: 1838 2048 1838
Size: 210 3605
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2023 2333 2479 3625 127
Size: 1896 310 146 1146 317
Fragments arranged by size:
1896 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 376 827 376
Size: 451 3364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 584 800 1640 2072 2819 3087 218
Size: 366 216 840 432 747 268 946
Fragments arranged by size:
946 840 747 432 366 268 216
EcoO109I rG'GnC_Cy
Cuts at: 346 954 346
Size: 608 3207
EcoRI G'AATT_C
Cuts at: 819 819
Size: 3815
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 717 810 1834 717
Size: 93 1024 2698
Fragments arranged by size:
2698 1024 93
FspI TGC'GCA
Cuts at: 1596 1596
Size: 3815
HaeII r_GCGC'y
Cuts at: 1249 1497 2519 3498 1249
Size: 248 1022 979 1566
Fragments arranged by size:
1566 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1657 1687 1689 1719 1657
Size: 30 2 30 3753
Fragments arranged by size:
3753 30 30 2
HincII GTy'rAC
Cuts at: 854 854
Size: 3815
HindIII A'AGCT_T
Cuts at: 699 699
Size: 3815
KasI G'GCGC_C
Cuts at: 1245 1493 1245
Size: 248 3567
KpnI G_GTAC'C
Cuts at: 835 835
Size: 3815
MluI A'CGCG_T
Cuts at: 3248 3248
Size: 3815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 502 717 799 2486 2670 502
Size: 215 82 1687 184 1647
Fragments arranged by size:
1687 1647 215 184 82
MscI TGG'CCA
Cuts at: 1576 1576
Size: 3815
MslI CAynn'nnrTG
Cuts at: 1804 1931 1804
Size: 127 3688
MspA1I CmG'CkG
Cuts at: 435 926 1240 1600 2613 2858 435
Size: 491 314 360 1013 245 1392
Fragments arranged by size:
1392 1013 491 360 314 245
NaeI GCC'GGC
Cuts at: 1996 1996
Size: 3815
NarI GG'CG_CC
Cuts at: 1246 1494 1246
Size: 248 3567
NcoI C'CATG_G
Cuts at: 107 107
Size: 3815
NgoMIV G'CCGG_C
Cuts at: 1994 1994
Size: 3815
NspI r_CATG'y
Cuts at: 868 1368 2275 868
Size: 500 907 2408
Fragments arranged by size:
2408 907 500
PciI A'CATG_T
Cuts at: 2271 2271
Size: 3815
PmeI GTTT'AAAC
Cuts at: 814 814
Size: 3815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2954 2986 2954
Size: 32 3783
PspOMI G'GGCC_C
Cuts at: 345 345
Size: 3815
PstI C_TGCA'G
Cuts at: 862 1547 862
Size: 685 3130
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3815
PvuII CAG'CTG
Cuts at: 435 1240 1600 435
Size: 805 360 2650
Fragments arranged by size:
2650 805 360
RsrII CG'GwC_CG
Cuts at: 2010 2010
Size: 3815
SacI G_AGCT'C
Cuts at: 378 829 378
Size: 451 3364
SalI G'TCGA_C
Cuts at: 852 852
Size: 3815
SapI GCTCTTCn'nnn_
Cuts at: 1838 2048 1838
Size: 210 3605
SbfI CC_TGCA'GG
Cuts at: 862 862
Size: 3815
ScaI AGT'ACT
Cuts at: 3332 3332
Size: 3815
SfcI C'TryA_G
Cuts at: 33 191 425 587 858 1543 2536 2727 33
Size: 158 234 162 271 685 993 191 1121
Fragments arranged by size:
1121 993 685 271 234 191 162 158
SfoI GGC'GCC
Cuts at: 1247 1495 1247
Size: 248 3567
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3815
SmaI CCC'GGG
Cuts at: 837 837
Size: 3815
SmlI C'TyrA_G
Cuts at: 628 970 1885 2377 2639 628
Size: 342 915 492 262 1804
Fragments arranged by size:
1804 915 492 342 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3815
SphI G_CATG'C
Cuts at: 868 1368 868
Size: 500 3315
StyI C'CwwG_G
Cuts at: 107 949 1303 107
Size: 842 354 2619
Fragments arranged by size:
2619 842 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1398 1531 1990 2199 1398
Size: 133 459 209 3014
Fragments arranged by size:
3014 459 209 133
TatI w'GTAC_w
Cuts at: 291 332 3330 291
Size: 41 2998 776
Fragments arranged by size:
2998 776 41
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3332 3364 3332
Size: 32 3783
Tth111I GACn'n_nGTC
Cuts at: 1612 1612
Size: 3815
XbaI T'CTAG_A
Cuts at: 846 846
Size: 3815
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1310 1310
Size: 3815
XmaI C'CCGG_G
Cuts at: 835 835
Size: 3815
XmnI GAAnn'nnTTC
Cuts at: 419 622 419
Size: 203 3612
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI