Restriction map for
pF1KB6948
(Circular) (Six-base) MAPSORT of: pF1KB6948.seq Check: 9194 from: 1 to: 3758
pF1KB6948 3758 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 796 796
Size: 3758
Acc65I G'GTAC_C
Cuts at: 774 774
Size: 3758
AclI AA'CG_TT
Cuts at: 3598 3598
Size: 3758
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1583 2015 2762 3030 1583
Size: 432 747 268 2311
Fragments arranged by size:
2311 747 432 268
AflIII A'CryG_T
Cuts at: 1110 2214 3191 1110
Size: 1104 977 1677
Fragments arranged by size:
1677 1104 977
AgeI A'CCGG_T
Cuts at: 2146 2146
Size: 3758
AlwNI CAG_nnn'CTG
Cuts at: 1063 2630 1063
Size: 1567 2191
ApaLI G'TGCA_C
Cuts at: 2528 2528
Size: 3758
ApoI r'AATT_y
Cuts at: 544 762 3111 3367 544
Size: 218 2349 256 935
Fragments arranged by size:
2349 935 256 218
AseI AT'TA_AT
Cuts at: 20 20
Size: 3758
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3758
AvaI C'yCGr_G
Cuts at: 778 778
Size: 3758
BamHI G'GATC_C
Cuts at: 329 783 329
Size: 454 3304
BanI G'GyrC_C
Cuts at: 774 1188 1436 1471 774
Size: 414 248 35 3061
Fragments arranged by size:
3061 414 248 35
BanII G_rGCy'C
Cuts at: 772 1802 772
Size: 1030 2728
BbeI G_GCGC'C
Cuts at: 1192 1440 1192
Size: 248 3510
BciVI GTATCCnnnnn_n'
Cuts at: 1656 2417 3758 1656
Size: 761 1341 1656
Fragments arranged by size:
1656 1341 761
BclI T'GATC_A
Cuts at: 1278 1278
Size: 3758
BglII A'GATC_T
Cuts at: 1273 3310 1273
Size: 2037 1721
BlpI GC'TnA_GC
Cuts at: 870 870
Size: 3758
Bme1580I G_kGCm'C
Cuts at: 1383 1476 2532 1383
Size: 93 1056 2609
Fragments arranged by size:
2609 1056 93
BmgBI CAC'GTC
Cuts at: 394 394
Size: 3758
BmrI ACTGGGnnnn_n'
Cuts at: 705 970 1051 1386 3530 705
Size: 265 81 335 2144 933
Fragments arranged by size:
2144 933 335 265 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2125 2125
Size: 3758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 492 293
Size: 199 3559
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 934 1813 2305 2603 934
Size: 879 492 298 2089
Fragments arranged by size:
2089 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3462 3462
Size: 3758
BsaAI yAC'GTr
Cuts at: 1111 1111
Size: 3758
BsaBI GATnn'nnATC
Cuts at: 6 1272 3309 6
Size: 1266 2037 455
Fragments arranged by size:
2037 1266 455
BsaHI Gr'CG_yC
Cuts at: 1189 1437 2139 3635 1189
Size: 248 702 1496 1312
Fragments arranged by size:
1496 1312 702 248
BsaWI w'CCGG_w
Cuts at: 947 1468 2146 2420 2567 2978 947
Size: 521 678 274 147 411 1727
Fragments arranged by size:
1727 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 430 430
Size: 3758
BseYI C'CCAG_C
Cuts at: 1183 1941 2518 3014 1183
Size: 758 577 496 1927
Fragments arranged by size:
1927 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 698 698
Size: 3758
BsiEI CG_ry'CG
Cuts at: 104 1300 1346 2554 104
Size: 1196 46 1208 1308
Fragments arranged by size:
1308 1208 1196 46
BsiHKAI G_wGCw'C
Cuts at: 772 1550 1740 2532 772
Size: 778 190 792 1998
Fragments arranged by size:
1998 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 772 1383 1476 1550 1740 1802 2532 772
Size: 611 93 74 190 62 730 1998
Fragments arranged by size:
1998 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2978 2978
Size: 3758
BspHI T'CATG_A
Cuts at: 2934 2934
Size: 3758
BspMI ACCTGCnnnn'nnnn_
Cuts at: 808 1022 1324 1705 808
Size: 214 302 381 2861
Fragments arranged by size:
2861 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2050 2104 3588 3753 50
Size: 2000 54 1484 165 55
Fragments arranged by size:
2000 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1670 1670
Size: 3758
BsrFI r'CCGG_y
Cuts at: 1756 1937 2146 1756
Size: 181 209 3368
Fragments arranged by size:
3368 209 181
BssSI C'ACGA_G
Cuts at: 2029 2387 2029
Size: 358 3400
BstAPI GCAn_nnn'nTGC
Cuts at: 1135 1135
Size: 3758
BstBI TT'CG_AA
Cuts at: 499 2119 499
Size: 1620 2138
BstXI CCAn_nnnn'nTGG
Cuts at: 572 572
Size: 3758
BstYI r'GATC_y
Cuts at: 1 329 718 783 1250 1273 1608 1854 2866
Size: 328 389 65 467 23 335 246 1012
Cuts at: 2866 2952 2964 3310 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 389 346 335 328 246
86 65 23 12
Bsu36I CC'TnA_GG
Cuts at: 413 413
Size: 3758
BtgI C'CryG_G
Cuts at: 107 607 107
Size: 500 3258
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1708 1889 2052 1708
Size: 181 163 3414
Fragments arranged by size:
3414 181 163
BtsI GCAGTG_nn'
Cuts at: 285 686 1001 285
Size: 401 315 3042
Fragments arranged by size:
3042 401 315
DraI TTT'AAA
Cuts at: 757 757
Size: 3758
DrdI GACnn_nn'nnGTC
Cuts at: 1464 2322 1464
Size: 858 2900
EaeI y'GGCC_r
Cuts at: 402 1343 1517 1908 1935 3280 402
Size: 941 174 391 27 1345 880
Fragments arranged by size:
1345 941 880 391 174 27
EagI C'GGCC_G
Cuts at: 1343 1343
Size: 3758
EarI CTCTTCn'nnn_
Cuts at: 470 1781 1991 470
Size: 1311 210 2237
Fragments arranged by size:
2237 1311 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1966 2276 2422 3568 1966
Size: 310 146 1146 2156
Fragments arranged by size:
2156 1146 310 146
EcoICRI GAG'CTC
Cuts at: 770 770
Size: 3758
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 492 1583 2015 2762 3030 293
Size: 199 1091 432 747 268 1021
Fragments arranged by size:
1091 1021 747 432 268 199
EcoO109I rG'GnC_Cy
Cuts at: 897 897
Size: 3758
EcoRI G'AATT_C
Cuts at: 762 762
Size: 3758
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 485 1777 485
Size: 1292 2466
FspI TGC'GCA
Cuts at: 1539 1539
Size: 3758
HaeII r_GCGC'y
Cuts at: 1192 1440 2462 3441 1192
Size: 248 1022 979 1509
Fragments arranged by size:
1509 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 286 318 1600 1630 1632 1662 286
Size: 32 1282 30 2 30 2382
Fragments arranged by size:
2382 1282 32 30 30 2
HincII GTy'rAC
Cuts at: 797 797
Size: 3758
HindIII A'AGCT_T
Cuts at: 248 748 248
Size: 500 3258
KasI G'GCGC_C
Cuts at: 1188 1436 1188
Size: 248 3510
KpnI G_GTAC'C
Cuts at: 778 778
Size: 3758
MluI A'CGCG_T
Cuts at: 3191 3191
Size: 3758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 458 2429 2613 458
Size: 1971 184 1603
Fragments arranged by size:
1971 1603 184
MscI TGG'CCA
Cuts at: 404 1519 404
Size: 1115 2643
MslI CAynn'nnrTG
Cuts at: 570 1747 1874 570
Size: 1177 127 2454
Fragments arranged by size:
2454 1177 127
MspA1I CmG'CkG
Cuts at: 869 1183 1543 2556 2801 869
Size: 314 360 1013 245 1826
Fragments arranged by size:
1826 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1939 1939
Size: 3758
NarI GG'CG_CC
Cuts at: 1189 1437 1189
Size: 248 3510
NcoI C'CATG_G
Cuts at: 107 607 107
Size: 500 3258
NgoMIV G'CCGG_C
Cuts at: 1937 1937
Size: 3758
NspI r_CATG'y
Cuts at: 811 1311 2218 811
Size: 500 907 2351
Fragments arranged by size:
2351 907 500
PciI A'CATG_T
Cuts at: 2214 2214
Size: 3758
PmeI GTTT'AAAC
Cuts at: 757 757
Size: 3758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2897 2929 2897
Size: 32 3726
PstI C_TGCA'G
Cuts at: 805 1490 805
Size: 685 3073
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3758
PvuII CAG'CTG
Cuts at: 1183 1543 1183
Size: 360 3398
RsrII CG'GwC_CG
Cuts at: 1953 1953
Size: 3758
SacI G_AGCT'C
Cuts at: 772 772
Size: 3758
SalI G'TCGA_C
Cuts at: 795 795
Size: 3758
SapI GCTCTTCn'nnn_
Cuts at: 1781 1991 1781
Size: 210 3548
SbfI CC_TGCA'GG
Cuts at: 805 805
Size: 3758
ScaI AGT'ACT
Cuts at: 3275 3275
Size: 3758
SfcI C'TryA_G
Cuts at: 33 521 801 1486 2479 2670 33
Size: 488 280 685 993 191 1121
Fragments arranged by size:
1121 993 685 488 280 191
SfoI GGC'GCC
Cuts at: 1190 1438 1190
Size: 248 3510
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3758
SmaI CCC'GGG
Cuts at: 780 780
Size: 3758
SmlI C'TyrA_G
Cuts at: 913 1828 2320 2582 913
Size: 915 492 262 2089
Fragments arranged by size:
2089 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3758
SphI G_CATG'C
Cuts at: 811 1311 811
Size: 500 3258
StyI C'CwwG_G
Cuts at: 107 117 607 892 1246 107
Size: 10 490 285 354 2619
Fragments arranged by size:
2619 490 354 285 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 600 1341 1474 1933 2142 600
Size: 741 133 459 209 2216
Fragments arranged by size:
2216 741 459 209 133
TatI w'GTAC_w
Cuts at: 493 3273 493
Size: 2780 978
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3275 3307 3275
Size: 32 3726
Tth111I GACn'n_nGTC
Cuts at: 1555 1555
Size: 3758
XbaI T'CTAG_A
Cuts at: 789 789
Size: 3758
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1253 1253
Size: 3758
XmaI C'CCGG_G
Cuts at: 778 778
Size: 3758
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI