Restriction map for
pF1KB6944
(Circular) (Six-base) MAPSORT of: pF1KB6944.seq Check: 7113 from: 1 to: 3806
pF1KB6944 3806 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 432 432
Size: 3806
AccI GT'mk_AC
Cuts at: 844 844
Size: 3806
Acc65I G'GTAC_C
Cuts at: 822 822
Size: 3806
AclI AA'CG_TT
Cuts at: 3646 3646
Size: 3806
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1631 2063 2810 3078 1631
Size: 432 747 268 2359
Fragments arranged by size:
2359 747 432 268
AflIII A'CryG_T
Cuts at: 674 1158 2262 3239 674
Size: 484 1104 977 1241
Fragments arranged by size:
1241 1104 977 484
AgeI A'CCGG_T
Cuts at: 2194 2194
Size: 3806
AleI CACnn'nnGTG
Cuts at: 145 145
Size: 3806
AlwNI CAG_nnn'CTG
Cuts at: 1111 2678 1111
Size: 1567 2239
ApaI G_GGCC'C
Cuts at: 615 615
Size: 3806
ApaLI G'TGCA_C
Cuts at: 643 2576 643
Size: 1933 1873
ApoI r'AATT_y
Cuts at: 810 3159 3415 810
Size: 2349 256 1201
Fragments arranged by size:
2349 1201 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3806
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3806
AvaI C'yCGr_G
Cuts at: 500 608 826 500
Size: 108 218 3480
Fragments arranged by size:
3480 218 108
BamHI G'GATC_C
Cuts at: 831 831
Size: 3806
BanI G'GyrC_C
Cuts at: 412 655 822 1236 1484 1519 412
Size: 243 167 414 248 35 2699
Fragments arranged by size:
2699 414 248 243 167 35
BanII G_rGCy'C
Cuts at: 290 615 729 794 820 1850 290
Size: 325 114 65 26 1030 2246
Fragments arranged by size:
2246 1030 325 114 65 26
BbeI G_GCGC'C
Cuts at: 1240 1488 1240
Size: 248 3558
BbsI GAAGACnn'nnnn_
Cuts at: 225 225
Size: 3806
BciVI GTATCCnnnnn_n'
Cuts at: 1704 2465 3806 1704
Size: 761 1341 1704
Fragments arranged by size:
1704 1341 761
BclI T'GATC_A
Cuts at: 1326 1326
Size: 3806
BglI GCCn_nnn'nGGC
Cuts at: 704 704
Size: 3806
BglII A'GATC_T
Cuts at: 275 1321 3358 275
Size: 1046 2037 723
Fragments arranged by size:
2037 1046 723
BlpI GC'TnA_GC
Cuts at: 918 918
Size: 3806
Bme1580I G_kGCm'C
Cuts at: 415 615 647 1431 1524 2580 415
Size: 200 32 784 93 1056 1641
Fragments arranged by size:
1641 1056 784 200 93 32
BmrI ACTGGGnnnn_n'
Cuts at: 1018 1099 1434 3578 1018
Size: 81 335 2144 1246
Fragments arranged by size:
2144 1246 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 309 2173 309
Size: 1864 1942
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 808 379
Size: 429 3377
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 1861 2353 2651 982
Size: 879 492 298 2137
Fragments arranged by size:
2137 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3510 3510
Size: 3806
BsaAI yAC'GTr
Cuts at: 1159 1159
Size: 3806
BsaBI GATnn'nnATC
Cuts at: 6 1320 3357 6
Size: 1314 2037 455
Fragments arranged by size:
2037 1314 455
BsaHI Gr'CG_yC
Cuts at: 1237 1485 2187 3683 1237
Size: 248 702 1496 1360
Fragments arranged by size:
1496 1360 702 248
BsaWI w'CCGG_w
Cuts at: 995 1516 2194 2468 2615 3026 995
Size: 521 678 274 147 411 1775
Fragments arranged by size:
1775 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 287 133
Size: 154 3652
BseYI C'CCAG_C
Cuts at: 126 513 1231 1989 2566 3062 126
Size: 387 718 758 577 496 870
Fragments arranged by size:
870 758 718 577 496 387
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 715 169
Size: 546 3260
BsiEI CG_ry'CG
Cuts at: 104 1348 1394 2602 104
Size: 1244 46 1208 1308
Fragments arranged by size:
1308 1244 1208 46
BsiHKAI G_wGCw'C
Cuts at: 290 647 780 820 1598 1788 2580 290
Size: 357 133 40 778 190 792 1516
Fragments arranged by size:
1516 792 778 357 190 133 40
Bsp1286I G_dGCh'C
Cuts at: 290 415 615 647 729 780 794 820 1431
Size: 125 200 32 82 51 14 26 611
Cuts at: 1431 1524 1598 1788 1850 2580 290
Size: 93 74 190 62 730 1516
Fragments arranged by size:
1516 730 611 200 190 125 93 82
74 62 51 32 26 14
BspEI T'CCGG_A
Cuts at: 3026 3026
Size: 3806
BspHI T'CATG_A
Cuts at: 669 2982 669
Size: 2313 1493
BspMI ACCTGCnnnn'nnnn_
Cuts at: 432 695 856 1070 1372 1753 432
Size: 263 161 214 302 381 2485
Fragments arranged by size:
2485 381 302 263 214 161
BsrBI CCG'CTC
Cuts at: 50 2098 2152 3636 3801 50
Size: 2048 54 1484 165 55
Fragments arranged by size:
2048 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1718 1718
Size: 3806
BsrFI r'CCGG_y
Cuts at: 144 1804 1985 2194 144
Size: 1660 181 209 1756
Fragments arranged by size:
1756 1660 209 181
BssSI C'ACGA_G
Cuts at: 2077 2435 2077
Size: 358 3448
BstAPI GCAn_nnn'nTGC
Cuts at: 738 1183 738
Size: 445 3361
BstBI TT'CG_AA
Cuts at: 2167 2167
Size: 3806
BstXI CCAn_nnnn'nTGG
Cuts at: 396 396
Size: 3806
BstYI r'GATC_y
Cuts at: 1 275 831 1298 1321 1656 1902 2914 3000
Size: 274 556 467 23 335 246 1012 86
Cuts at: 3000 3012 3358 1
Size: 12 346 449
Fragments arranged by size:
1012 556 467 449 346 335 274 246
86 23 12
BtgI C'CryG_G
Cuts at: 423 423
Size: 3806
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1756 1937 2100 1756
Size: 181 163 3462
Fragments arranged by size:
3462 181 163
BtsI GCAGTG_nn'
Cuts at: 1049 1049
Size: 3806
DraI TTT'AAA
Cuts at: 805 805
Size: 3806
DrdI GACnn_nn'nnGTC
Cuts at: 250 1512 2370 250
Size: 1262 858 1686
Fragments arranged by size:
1686 1262 858
EaeI y'GGCC_r
Cuts at: 600 1391 1565 1956 1983 3328 600
Size: 791 174 391 27 1345 1078
Fragments arranged by size:
1345 1078 791 391 174 27
EagI C'GGCC_G
Cuts at: 1391 1391
Size: 3806
EarI CTCTTCn'nnn_
Cuts at: 295 1829 2039 295
Size: 1534 210 2062
Fragments arranged by size:
2062 1534 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2014 2324 2470 3616 2014
Size: 310 146 1146 2204
Fragments arranged by size:
2204 1146 310 146
EcoICRI GAG'CTC
Cuts at: 288 818 288
Size: 530 3276
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 808 1631 2063 2810 3078 379
Size: 429 823 432 747 268 1107
Fragments arranged by size:
1107 823 747 432 429 268
EcoO109I rG'GnC_Cy
Cuts at: 945 945
Size: 3806
EcoRI G'AATT_C
Cuts at: 810 810
Size: 3806
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1825 1825
Size: 3806
FspI TGC'GCA
Cuts at: 1587 1587
Size: 3806
HaeII r_GCGC'y
Cuts at: 174 1240 1488 2510 3489 174
Size: 1066 248 1022 979 491
Fragments arranged by size:
1066 1022 979 491 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1648 1678 1680 1710 1648
Size: 30 2 30 3744
Fragments arranged by size:
3744 30 30 2
HincII GTy'rAC
Cuts at: 845 845
Size: 3806
KasI G'GCGC_C
Cuts at: 1236 1484 1236
Size: 248 3558
KpnI G_GTAC'C
Cuts at: 826 826
Size: 3806
MluI A'CGCG_T
Cuts at: 3239 3239
Size: 3806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2477 2661 2477
Size: 184 3622
MscI TGG'CCA
Cuts at: 602 1567 602
Size: 965 2841
MslI CAynn'nnrTG
Cuts at: 145 650 1795 1922 145
Size: 505 1145 127 2029
Fragments arranged by size:
2029 1145 505 127
MspA1I CmG'CkG
Cuts at: 917 1231 1591 2604 2849 917
Size: 314 360 1013 245 1874
Fragments arranged by size:
1874 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1987 1987
Size: 3806
NarI GG'CG_CC
Cuts at: 1237 1485 1237
Size: 248 3558
NgoMIV G'CCGG_C
Cuts at: 1985 1985
Size: 3806
NspI r_CATG'y
Cuts at: 307 346 678 859 1359 2266 307
Size: 39 332 181 500 907 1847
Fragments arranged by size:
1847 907 500 332 181 39
PciI A'CATG_T
Cuts at: 674 2262 674
Size: 1588 2218
PmeI GTTT'AAAC
Cuts at: 805 805
Size: 3806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2945 2977 2945
Size: 32 3774
PspOMI G'GGCC_C
Cuts at: 611 611
Size: 3806
PstI C_TGCA'G
Cuts at: 853 1538 853
Size: 685 3121
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3806
PvuII CAG'CTG
Cuts at: 1231 1591 1231
Size: 360 3446
RsrII CG'GwC_CG
Cuts at: 2001 2001
Size: 3806
SacI G_AGCT'C
Cuts at: 290 820 290
Size: 530 3276
SalI G'TCGA_C
Cuts at: 843 843
Size: 3806
SapI GCTCTTCn'nnn_
Cuts at: 295 1829 2039 295
Size: 1534 210 2062
Fragments arranged by size:
2062 1534 210
SbfI CC_TGCA'GG
Cuts at: 853 853
Size: 3806
ScaI AGT'ACT
Cuts at: 3323 3323
Size: 3806
SfcI C'TryA_G
Cuts at: 33 849 1534 2527 2718 33
Size: 816 685 993 191 1121
Fragments arranged by size:
1121 993 816 685 191
SfoI GGC'GCC
Cuts at: 1238 1486 1238
Size: 248 3558
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3806
SgrAI Cr'CCGG_yG
Cuts at: 144 144
Size: 3806
SmaI CCC'GGG
Cuts at: 502 828 502
Size: 326 3480
SmlI C'TyrA_G
Cuts at: 961 1876 2368 2630 961
Size: 915 492 262 2137
Fragments arranged by size:
2137 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3806
SphI G_CATG'C
Cuts at: 307 859 1359 307
Size: 552 500 2754
Fragments arranged by size:
2754 552 500
StyI C'CwwG_G
Cuts at: 248 771 940 1294 248
Size: 523 169 354 2760
Fragments arranged by size:
2760 523 354 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 396 1389 1522 1981 2190 396
Size: 993 133 459 209 2012
Fragments arranged by size:
2012 993 459 209 133
TatI w'GTAC_w
Cuts at: 345 3321 345
Size: 2976 830
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3323 3355 3323
Size: 32 3774
Tth111I GACn'n_nGTC
Cuts at: 1603 1603
Size: 3806
XbaI T'CTAG_A
Cuts at: 837 837
Size: 3806
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 396 471 1301 396
Size: 75 830 2901
Fragments arranged by size:
2901 830 75
XmaI C'CCGG_G
Cuts at: 500 826 500
Size: 326 3480
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI