Restriction map for
pF1KB6924
(Circular) (Six-base) MAPSORT of: pF1KB6924.seq Check: 5101 from: 1 to: 3794
pF1KB6924 3794 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 832 832
Size: 3794
Acc65I G'GTAC_C
Cuts at: 810 810
Size: 3794
AclI AA'CG_TT
Cuts at: 3634 3634
Size: 3794
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1619 2051 2798 3066 1619
Size: 432 747 268 2347
Fragments arranged by size:
2347 747 432 268
AflIII A'CryG_T
Cuts at: 1146 2250 3227 1146
Size: 1104 977 1713
Fragments arranged by size:
1713 1104 977
AgeI A'CCGG_T
Cuts at: 2182 2182
Size: 3794
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 670 702 670
Size: 32 3762
AlwNI CAG_nnn'CTG
Cuts at: 1099 2666 1099
Size: 1567 2227
ApaI G_GGCC'C
Cuts at: 410 410
Size: 3794
ApaLI G'TGCA_C
Cuts at: 2564 2564
Size: 3794
ApoI r'AATT_y
Cuts at: 798 3147 3403 798
Size: 2349 256 1189
Fragments arranged by size:
2349 1189 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3794
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3794
AvaI C'yCGr_G
Cuts at: 327 814 327
Size: 487 3307
BamHI G'GATC_C
Cuts at: 819 819
Size: 3794
BanI G'GyrC_C
Cuts at: 530 705 810 1224 1472 1507 530
Size: 175 105 414 248 35 2817
Fragments arranged by size:
2817 414 248 175 105 35
BanII G_rGCy'C
Cuts at: 410 808 1838 410
Size: 398 1030 2366
Fragments arranged by size:
2366 1030 398
BbeI G_GCGC'C
Cuts at: 534 1228 1476 534
Size: 694 248 2852
Fragments arranged by size:
2852 694 248
BbvCI CC'TCA_GC
Cuts at: 164 164
Size: 3794
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 598 632 90
Size: 34 474 34 3252
Fragments arranged by size:
3252 474 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 339 1692 2453 3794 339
Size: 1353 761 1341 339
Fragments arranged by size:
1353 1341 761 339
BclI T'GATC_A
Cuts at: 1314 1314
Size: 3794
BglII A'GATC_T
Cuts at: 1309 3346 1309
Size: 2037 1757
BlpI GC'TnA_GC
Cuts at: 906 906
Size: 3794
Bme1580I G_kGCm'C
Cuts at: 410 497 662 710 1419 1512 2568 410
Size: 87 165 48 709 93 1056 1636
Fragments arranged by size:
1636 1056 709 165 93 87 48
BmgBI CAC'GTC
Cuts at: 657 657
Size: 3794
BmrI ACTGGGnnnn_n'
Cuts at: 1006 1087 1422 3566 1006
Size: 81 335 2144 1234
Fragments arranged by size:
2144 1234 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2161 2161
Size: 3794
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 519 649 519
Size: 130 3664
Bpu10I CC'TnA_GC
Cuts at: 164 164
Size: 3794
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 261 970 1849 2341 2639 261
Size: 709 879 492 298 1416
Fragments arranged by size:
1416 879 709 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3498 3498
Size: 3794
BsaAI yAC'GTr
Cuts at: 1147 1147
Size: 3794
BsaBI GATnn'nnATC
Cuts at: 6 1308 3345 6
Size: 1302 2037 455
Fragments arranged by size:
2037 1302 455
BsaHI Gr'CG_yC
Cuts at: 364 531 1225 1473 2175 3671 364
Size: 167 694 248 702 1496 487
Fragments arranged by size:
1496 702 694 487 248 167
BsaWI w'CCGG_w
Cuts at: 333 596 983 1504 2182 2456 2603 3014 333
Size: 263 387 521 678 274 147 411 1113
Fragments arranged by size:
1113 678 521 411 387 274 263 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 670 700 670
Size: 30 3764
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 164 646 649 763 164
Size: 482 3 114 3195
Fragments arranged by size:
3195 482 114 3
BseYI C'CCAG_C
Cuts at: 701 1219 1977 2554 3050 701
Size: 518 758 577 496 1445
Fragments arranged by size:
1445 758 577 518 496
BsiEI CG_ry'CG
Cuts at: 104 226 597 1336 1382 2590 104
Size: 122 371 739 46 1208 1308
Fragments arranged by size:
1308 1208 739 371 122 46
BsiHKAI G_wGCw'C
Cuts at: 808 1586 1776 2568 808
Size: 778 190 792 2034
Fragments arranged by size:
2034 792 778 190
BsmI GAATG_Cn'
Cuts at: 184 184
Size: 3794
Bsp1286I G_dGCh'C
Cuts at: 410 497 662 710 808 1419 1512 1586 1776
Size: 87 165 48 98 611 93 74 190
Cuts at: 1776 1838 2568 410
Size: 62 730 1636
Fragments arranged by size:
1636 730 611 190 165 98 93 87
74 62 48
BspEI T'CCGG_A
Cuts at: 3014 3014
Size: 3794
BspHI T'CATG_A
Cuts at: 647 2970 647
Size: 2323 1471
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 1058 1360 1741 844
Size: 214 302 381 2897
Fragments arranged by size:
2897 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2086 2140 3624 3789 50
Size: 2036 54 1484 165 55
Fragments arranged by size:
2036 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1706 1706
Size: 3794
BsrFI r'CCGG_y
Cuts at: 1792 1973 2182 1792
Size: 181 209 3404
Fragments arranged by size:
3404 209 181
BssSI C'ACGA_G
Cuts at: 2065 2423 2065
Size: 358 3436
BstAPI GCAn_nnn'nTGC
Cuts at: 384 1171 384
Size: 787 3007
BstBI TT'CG_AA
Cuts at: 2155 2155
Size: 3794
BstYI r'GATC_y
Cuts at: 1 819 1286 1309 1644 1890 2902 2988 3000
Size: 818 467 23 335 246 1012 86 12
Cuts at: 3000 3346 1
Size: 346 449
Fragments arranged by size:
1012 818 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 265 768 265
Size: 503 3291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 546 1744 1925 2088 546
Size: 1198 181 163 2252
Fragments arranged by size:
2252 1198 181 163
BtsI GCAGTG_nn'
Cuts at: 1037 1037
Size: 3794
DraI TTT'AAA
Cuts at: 793 793
Size: 3794
DrdI GACnn_nn'nnGTC
Cuts at: 1500 2358 1500
Size: 858 2936
EaeI y'GGCC_r
Cuts at: 464 1379 1553 1944 1971 3316 464
Size: 915 174 391 27 1345 942
Fragments arranged by size:
1345 942 915 391 174 27
EagI C'GGCC_G
Cuts at: 1379 1379
Size: 3794
EarI CTCTTCn'nnn_
Cuts at: 1817 2027 1817
Size: 210 3584
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 190 276 313 380 2002 2312 2458 3604 190
Size: 86 37 67 1622 310 146 1146 380
Fragments arranged by size:
1622 1146 380 310 146 86 67 37
EcoICRI GAG'CTC
Cuts at: 806 806
Size: 3794
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 519 649 1619 2051 2798 3066 519
Size: 130 970 432 747 268 1247
Fragments arranged by size:
1247 970 747 432 268 130
EcoNI CCTnn'n_nnAGG
Cuts at: 631 631
Size: 3794
EcoO109I rG'GnC_Cy
Cuts at: 602 933 602
Size: 331 3463
EcoRI G'AATT_C
Cuts at: 798 798
Size: 3794
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1813 1813
Size: 3794
FspI TGC'GCA
Cuts at: 739 1575 739
Size: 836 2958
HaeII r_GCGC'y
Cuts at: 534 1228 1476 2498 3477 534
Size: 694 248 1022 979 851
Fragments arranged by size:
1022 979 851 694 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1636 1666 1668 1698 1636
Size: 30 2 30 3732
Fragments arranged by size:
3732 30 30 2
HincII GTy'rAC
Cuts at: 833 833
Size: 3794
KasI G'GCGC_C
Cuts at: 530 1224 1472 530
Size: 694 248 2852
Fragments arranged by size:
2852 694 248
KpnI G_GTAC'C
Cuts at: 814 814
Size: 3794
MluI A'CGCG_T
Cuts at: 3227 3227
Size: 3794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 206 2465 2649 206
Size: 2259 184 1351
Fragments arranged by size:
2259 1351 184
MscI TGG'CCA
Cuts at: 466 1555 466
Size: 1089 2705
MslI CAynn'nnrTG
Cuts at: 304 646 1783 1910 304
Size: 342 1137 127 2188
Fragments arranged by size:
2188 1137 342 127
MspA1I CmG'CkG
Cuts at: 168 267 701 905 1219 1579 2592 2837 168
Size: 99 434 204 314 360 1013 245 1125
Fragments arranged by size:
1125 1013 434 360 314 245 204 99
NaeI GCC'GGC
Cuts at: 1975 1975
Size: 3794
NarI GG'CG_CC
Cuts at: 531 1225 1473 531
Size: 694 248 2852
Fragments arranged by size:
2852 694 248
NgoMIV G'CCGG_C
Cuts at: 1973 1973
Size: 3794
NspI r_CATG'y
Cuts at: 387 462 847 1347 2254 387
Size: 75 385 500 907 1927
Fragments arranged by size:
1927 907 500 385 75
PasI CC'CwG_GG
Cuts at: 194 194
Size: 3794
PciI A'CATG_T
Cuts at: 2250 2250
Size: 3794
PfoI T'CCnGG_A
Cuts at: 245 245
Size: 3794
PmeI GTTT'AAAC
Cuts at: 793 793
Size: 3794
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 670 702 2933 2965 670
Size: 32 2231 32 1499
Fragments arranged by size:
2231 1499 32 32
PpuMI rG'GwC_Cy
Cuts at: 602 602
Size: 3794
PspOMI G'GGCC_C
Cuts at: 406 406
Size: 3794
PstI C_TGCA'G
Cuts at: 173 841 1526 173
Size: 668 685 2441
Fragments arranged by size:
2441 685 668
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3794
PvuII CAG'CTG
Cuts at: 168 1219 1579 168
Size: 1051 360 2383
Fragments arranged by size:
2383 1051 360
RsrII CG'GwC_CG
Cuts at: 1989 1989
Size: 3794
SacI G_AGCT'C
Cuts at: 808 808
Size: 3794
SacII CC_GC'GG
Cuts at: 268 268
Size: 3794
SalI G'TCGA_C
Cuts at: 831 831
Size: 3794
SapI GCTCTTCn'nnn_
Cuts at: 1817 2027 1817
Size: 210 3584
SbfI CC_TGCA'GG
Cuts at: 841 841
Size: 3794
ScaI AGT'ACT
Cuts at: 3311 3311
Size: 3794
SfcI C'TryA_G
Cuts at: 33 169 453 837 1522 2515 2706 33
Size: 136 284 384 685 993 191 1121
Fragments arranged by size:
1121 993 685 384 284 191 136
SfoI GGC'GCC
Cuts at: 532 1226 1474 532
Size: 694 248 2852
Fragments arranged by size:
2852 694 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3794
SmaI CCC'GGG
Cuts at: 329 816 329
Size: 487 3307
SmlI C'TyrA_G
Cuts at: 276 949 1864 2356 2618 276
Size: 673 915 492 262 1452
Fragments arranged by size:
1452 915 673 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3794
SphI G_CATG'C
Cuts at: 462 847 1347 462
Size: 385 500 2909
Fragments arranged by size:
2909 500 385
StyI C'CwwG_G
Cuts at: 118 928 1282 118
Size: 810 354 2630
Fragments arranged by size:
2630 810 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 693 1377 1510 1969 2178 693
Size: 684 133 459 209 2309
Fragments arranged by size:
2309 684 459 209 133
TatI w'GTAC_w
Cuts at: 3309 3309
Size: 3794
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 323 355 3311 3343 323
Size: 32 2956 32 774
Fragments arranged by size:
2956 774 32 32
Tth111I GACn'n_nGTC
Cuts at: 1591 1591
Size: 3794
XbaI T'CTAG_A
Cuts at: 825 825
Size: 3794
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 297 1289 297
Size: 992 2802
XmaI C'CCGG_G
Cuts at: 327 814 327
Size: 487 3307
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbsI BfrBI BglI BmtI BsgI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PshAI PsiI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI