Restriction map for
pF1KB6912
(Circular) (Six-base) MAPSORT of: pF1KB6912.seq Check: 9691 from: 1 to: 3767
pF1KB6912 3767 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AatII G_ACGT'C
Cuts at: 281 281
Size: 3767
AccI GT'mk_AC
Cuts at: 805 805
Size: 3767
Acc65I G'GTAC_C
Cuts at: 783 783
Size: 3767
AclI AA'CG_TT
Cuts at: 3607 3607
Size: 3767
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1592 2024 2771 3039 1592
Size: 432 747 268 2320
Fragments arranged by size:
2320 747 432 268
AflIII A'CryG_T
Cuts at: 504 1119 2223 3200 504
Size: 615 1104 977 1071
Fragments arranged by size:
1104 1071 977 615
AgeI A'CCGG_T
Cuts at: 2155 2155
Size: 3767
AleI CACnn'nnGTG
Cuts at: 403 403
Size: 3767
AlwNI CAG_nnn'CTG
Cuts at: 1072 2639 1072
Size: 1567 2200
ApaLI G'TGCA_C
Cuts at: 2537 2537
Size: 3767
ApoI r'AATT_y
Cuts at: 771 3120 3376 771
Size: 2349 256 1162
Fragments arranged by size:
2349 1162 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3767
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3767
AvaI C'yCGr_G
Cuts at: 787 787
Size: 3767
BamHI G'GATC_C
Cuts at: 792 792
Size: 3767
BanI G'GyrC_C
Cuts at: 392 531 573 751 783 1197 1445 1480 392
Size: 139 42 178 32 414 248 35 2679
Fragments arranged by size:
2679 414 248 178 139 42 35 32
BanII G_rGCy'C
Cuts at: 674 781 1811 674
Size: 107 1030 2630
Fragments arranged by size:
2630 1030 107
BbeI G_GCGC'C
Cuts at: 396 577 1201 1449 396
Size: 181 624 248 2714
Fragments arranged by size:
2714 624 248 181
BbsI GAAGACnn'nnnn_
Cuts at: 481 587 737 481
Size: 106 150 3511
Fragments arranged by size:
3511 150 106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 741 775 741
Size: 34 3733
BciVI GTATCCnnnnn_n'
Cuts at: 686 1665 2426 3767 686
Size: 979 761 1341 686
Fragments arranged by size:
1341 979 761 686
BclI T'GATC_A
Cuts at: 1287 1287
Size: 3767
BglII A'GATC_T
Cuts at: 1282 3319 1282
Size: 2037 1730
BlpI GC'TnA_GC
Cuts at: 879 879
Size: 3767
Bme1580I G_kGCm'C
Cuts at: 536 754 1392 1485 2541 536
Size: 218 638 93 1056 1762
Fragments arranged by size:
1762 1056 638 218 93
BmgBI CAC'GTC
Cuts at: 507 507
Size: 3767
BmrI ACTGGGnnnn_n'
Cuts at: 979 1060 1395 3539 979
Size: 81 335 2144 1207
Fragments arranged by size:
2144 1207 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2134 2134
Size: 3767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 1822 2314 2612 943
Size: 879 492 298 2098
Fragments arranged by size:
2098 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 217 3471 217
Size: 3254 513
BsaAI yAC'GTr
Cuts at: 1120 1120
Size: 3767
BsaBI GATnn'nnATC
Cuts at: 6 1281 3318 6
Size: 1275 2037 455
Fragments arranged by size:
2037 1275 455
BsaHI Gr'CG_yC
Cuts at: 249 278 393 574 1198 1446 2148 3644 249
Size: 29 115 181 624 248 702 1496 372
Fragments arranged by size:
1496 702 624 372 248 181 115 29
BsaWI w'CCGG_w
Cuts at: 956 1477 2155 2429 2576 2987 956
Size: 521 678 274 147 411 1736
Fragments arranged by size:
1736 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 559 562 604 682 559
Size: 3 42 78 3644
Fragments arranged by size:
3644 78 42 3
BseYI C'CCAG_C
Cuts at: 200 1192 1950 2527 3023 200
Size: 992 758 577 496 944
Fragments arranged by size:
992 944 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 706
Size: 3767
BsiEI CG_ry'CG
Cuts at: 104 238 315 1309 1355 2563 104
Size: 134 77 994 46 1208 1308
Fragments arranged by size:
1308 1208 994 134 77 46
BsiHKAI G_wGCw'C
Cuts at: 781 1559 1749 2541 781
Size: 778 190 792 2007
Fragments arranged by size:
2007 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 536 674 754 781 1392 1485 1559 1749 1811
Size: 138 80 27 611 93 74 190 62
Cuts at: 1811 2541 536
Size: 730 1762
Fragments arranged by size:
1762 730 611 190 138 93 80 74
62 27
BspEI T'CCGG_A
Cuts at: 2987 2987
Size: 3767
BspHI T'CATG_A
Cuts at: 2943 2943
Size: 3767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 817 1031 1333 1714 817
Size: 214 302 381 2870
Fragments arranged by size:
2870 381 302 214
BsrBI CCG'CTC
Cuts at: 50 324 2059 2113 3597 3762 50
Size: 274 1735 54 1484 165 55
Fragments arranged by size:
1735 1484 274 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1679 1679
Size: 3767
BsrFI r'CCGG_y
Cuts at: 251 258 570 1765 1946 2155 251
Size: 7 312 1195 181 209 1863
Fragments arranged by size:
1863 1195 312 209 181 7
BssHII G'CGCG_C
Cuts at: 162 162
Size: 3767
BssSI C'ACGA_G
Cuts at: 541 2038 2396 541
Size: 1497 358 1912
Fragments arranged by size:
1912 1497 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1144 1144
Size: 3767
BstBI TT'CG_AA
Cuts at: 2128 2128
Size: 3767
BstYI r'GATC_y
Cuts at: 1 792 1259 1282 1617 1863 2875 2961 2973
Size: 791 467 23 335 246 1012 86 12
Cuts at: 2973 3319 1
Size: 346 449
Fragments arranged by size:
1012 791 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 130 438 107
Size: 23 308 3436
Fragments arranged by size:
3436 308 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1717 1898 2061 1717
Size: 181 163 3423
Fragments arranged by size:
3423 181 163
BtsI GCAGTG_nn'
Cuts at: 1010 1010
Size: 3767
DraI TTT'AAA
Cuts at: 766 766
Size: 3767
DrdI GACnn_nn'nnGTC
Cuts at: 1473 2331 1473
Size: 858 2909
EaeI y'GGCC_r
Cuts at: 235 312 1352 1526 1917 1944 3289 235
Size: 77 1040 174 391 27 1345 713
Fragments arranged by size:
1345 1040 713 391 174 77 27
EagI C'GGCC_G
Cuts at: 235 312 1352 235
Size: 77 1040 2650
Fragments arranged by size:
2650 1040 77
EarI CTCTTCn'nnn_
Cuts at: 331 1790 2000 331
Size: 1459 210 2098
Fragments arranged by size:
2098 1459 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 271 1975 2285 2431 3577 271
Size: 1704 310 146 1146 461
Fragments arranged by size:
1704 1146 461 310 146
EcoICRI GAG'CTC
Cuts at: 779 779
Size: 3767
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1592 2024 2771 3039 1592
Size: 432 747 268 2320
Fragments arranged by size:
2320 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 906 906
Size: 3767
EcoRI G'AATT_C
Cuts at: 771 771
Size: 3767
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1786 1786
Size: 3767
FspI TGC'GCA
Cuts at: 1548 1548
Size: 3767
HaeII r_GCGC'y
Cuts at: 396 577 1201 1449 2471 3450 396
Size: 181 624 248 1022 979 713
Fragments arranged by size:
1022 979 713 624 248 181
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1609 1639 1641 1671 1609
Size: 30 2 30 3705
Fragments arranged by size:
3705 30 30 2
HincII GTy'rAC
Cuts at: 806 806
Size: 3767
KasI G'GCGC_C
Cuts at: 392 573 1197 1445 392
Size: 181 624 248 2714
Fragments arranged by size:
2714 624 248 181
KpnI G_GTAC'C
Cuts at: 787 787
Size: 3767
MluI A'CGCG_T
Cuts at: 3200 3200
Size: 3767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2438 2622 2438
Size: 184 3583
MscI TGG'CCA
Cuts at: 1528 1528
Size: 3767
MslI CAynn'nnrTG
Cuts at: 403 1756 1883 403
Size: 1353 127 2287
Fragments arranged by size:
2287 1353 127
MspA1I CmG'CkG
Cuts at: 132 306 878 1192 1552 2565 2810 132
Size: 174 572 314 360 1013 245 1089
Fragments arranged by size:
1089 1013 572 360 314 245 174
NaeI GCC'GGC
Cuts at: 253 572 1948 253
Size: 319 1376 2072
Fragments arranged by size:
2072 1376 319
NarI GG'CG_CC
Cuts at: 393 574 1198 1446 393
Size: 181 624 248 2714
Fragments arranged by size:
2714 624 248 181
NcoI C'CATG_G
Cuts at: 107 107
Size: 3767
NgoMIV G'CCGG_C
Cuts at: 251 570 1946 251
Size: 319 1376 2072
Fragments arranged by size:
2072 1376 319
NspI r_CATG'y
Cuts at: 820 1320 2227 820
Size: 500 907 2360
Fragments arranged by size:
2360 907 500
PasI CC'CwG_GG
Cuts at: 707 707
Size: 3767
PciI A'CATG_T
Cuts at: 2223 2223
Size: 3767
PmeI GTTT'AAAC
Cuts at: 766 766
Size: 3767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2906 2938 2906
Size: 32 3735
PstI C_TGCA'G
Cuts at: 814 1499 814
Size: 685 3082
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3767
PvuII CAG'CTG
Cuts at: 306 1192 1552 306
Size: 886 360 2521
Fragments arranged by size:
2521 886 360
RsrII CG'GwC_CG
Cuts at: 261 1962 261
Size: 1701 2066
SacI G_AGCT'C
Cuts at: 781 781
Size: 3767
SacII CC_GC'GG
Cuts at: 133 133
Size: 3767
SalI G'TCGA_C
Cuts at: 804 804
Size: 3767
SapI GCTCTTCn'nnn_
Cuts at: 331 1790 2000 331
Size: 1459 210 2098
Fragments arranged by size:
2098 1459 210
SbfI CC_TGCA'GG
Cuts at: 814 814
Size: 3767
ScaI AGT'ACT
Cuts at: 3284 3284
Size: 3767
SfcI C'TryA_G
Cuts at: 33 810 1495 2488 2679 33
Size: 777 685 993 191 1121
Fragments arranged by size:
1121 993 777 685 191
SfoI GGC'GCC
Cuts at: 394 575 1199 1447 394
Size: 181 624 248 2714
Fragments arranged by size:
2714 624 248 181
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3767
SgrAI Cr'CCGG_yG
Cuts at: 570 570
Size: 3767
SmaI CCC'GGG
Cuts at: 789 789
Size: 3767
SmlI C'TyrA_G
Cuts at: 922 1837 2329 2591 922
Size: 915 492 262 2098
Fragments arranged by size:
2098 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3767
SphI G_CATG'C
Cuts at: 820 1320 820
Size: 500 3267
StuI AGG'CCT
Cuts at: 448 553 448
Size: 105 3662
StyI C'CwwG_G
Cuts at: 107 746 901 1255 107
Size: 639 155 354 2619
Fragments arranged by size:
2619 639 354 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1350 1483 1942 2151 1350
Size: 133 459 209 2966
Fragments arranged by size:
2966 459 209 133
TatI w'GTAC_w
Cuts at: 3282 3282
Size: 3767
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3284 3316 3284
Size: 32 3735
Tth111I GACn'n_nGTC
Cuts at: 1564 1564
Size: 3767
XbaI T'CTAG_A
Cuts at: 798 798
Size: 3767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1262 1262
Size: 3767
XmaI C'CCGG_G
Cuts at: 787 787
Size: 3767
ZraI GAC'GTC
Cuts at: 279 279
Size: 3767
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmtI BpmI Bpu10I
BsaXI BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SfiI SnaBI SrfI SspI
SwaI XhoI XmnI