Restriction map for
pF1KB6911
(Circular) (Six-base) MAPSORT of: pF1KB6911.seq Check: 7856 from: 1 to: 3728
pF1KB6911 3728 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 538 538
Size: 3728
AatII G_ACGT'C
Cuts at: 287 287
Size: 3728
AccI GT'mk_AC
Cuts at: 766 766
Size: 3728
Acc65I G'GTAC_C
Cuts at: 744 744
Size: 3728
AclI AA'CG_TT
Cuts at: 3568 3568
Size: 3728
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 1553 1985 2732 3000 727
Size: 826 432 747 268 1455
Fragments arranged by size:
1455 826 747 432 268
AflIII A'CryG_T
Cuts at: 1080 2184 3161 1080
Size: 1104 977 1647
Fragments arranged by size:
1647 1104 977
AgeI A'CCGG_T
Cuts at: 2116 2116
Size: 3728
AlwNI CAG_nnn'CTG
Cuts at: 1033 2600 1033
Size: 1567 2161
ApaLI G'TGCA_C
Cuts at: 2498 2498
Size: 3728
ApoI r'AATT_y
Cuts at: 732 3081 3337 732
Size: 2349 256 1123
Fragments arranged by size:
2349 1123 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3728
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
AvaI C'yCGr_G
Cuts at: 748 748
Size: 3728
BamHI G'GATC_C
Cuts at: 194 582 753 194
Size: 388 171 3169
Fragments arranged by size:
3169 388 171
BanI G'GyrC_C
Cuts at: 526 744 1158 1406 1441 526
Size: 218 414 248 35 2813
Fragments arranged by size:
2813 414 248 218 35
BanII G_rGCy'C
Cuts at: 275 575 742 1772 275
Size: 300 167 1030 2231
Fragments arranged by size:
2231 1030 300 167
BbeI G_GCGC'C
Cuts at: 1162 1410 1162
Size: 248 3480
BbsI GAAGACnn'nnnn_
Cuts at: 457 457
Size: 3728
BciVI GTATCCnnnnn_n'
Cuts at: 1626 2387 3728 1626
Size: 761 1341 1626
Fragments arranged by size:
1626 1341 761
BclI T'GATC_A
Cuts at: 1248 1248
Size: 3728
BglI GCCn_nnn'nGGC
Cuts at: 142 142
Size: 3728
BglII A'GATC_T
Cuts at: 230 1243 3280 230
Size: 1013 2037 678
Fragments arranged by size:
2037 1013 678
BlpI GC'TnA_GC
Cuts at: 840 840
Size: 3728
Bme1580I G_kGCm'C
Cuts at: 1353 1446 2502 1353
Size: 93 1056 2579
Fragments arranged by size:
2579 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 940 1021 1356 3500 940
Size: 81 335 2144 1168
Fragments arranged by size:
2144 1168 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 3728
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 904 1783 2275 2573 292
Size: 612 879 492 298 1447
Fragments arranged by size:
1447 879 612 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3432 3432
Size: 3728
BsaAI yAC'GTr
Cuts at: 1081 1081
Size: 3728
BsaBI GATnn'nnATC
Cuts at: 6 587 1242 3279 6
Size: 581 655 2037 455
Fragments arranged by size:
2037 655 581 455
BsaHI Gr'CG_yC
Cuts at: 284 1159 1407 2109 3605 284
Size: 875 248 702 1496 407
Fragments arranged by size:
1496 875 702 407 248
BsaWI w'CCGG_w
Cuts at: 585 917 1438 2116 2390 2537 2948 585
Size: 332 521 678 274 147 411 1365
Fragments arranged by size:
1365 678 521 411 332 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 122 152 650 680 122
Size: 30 498 30 3170
Fragments arranged by size:
3170 498 30 30
BseYI C'CCAG_C
Cuts at: 299 1153 1911 2488 2984 299
Size: 854 758 577 496 1043
Fragments arranged by size:
1043 854 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1270 1316 2524 104
Size: 1166 46 1208 1308
Fragments arranged by size:
1308 1208 1166 46
BsiHKAI G_wGCw'C
Cuts at: 742 1520 1710 2502 742
Size: 778 190 792 1968
Fragments arranged by size:
1968 792 778 190
BsmI GAATG_Cn'
Cuts at: 630 630
Size: 3728
Bsp1286I G_dGCh'C
Cuts at: 275 575 742 1353 1446 1520 1710 1772 2502
Size: 300 167 611 93 74 190 62 730
Cuts at: 2502 275
Size: 1501
Fragments arranged by size:
1501 730 611 300 190 167 93 74
62
BspEI T'CCGG_A
Cuts at: 585 2948 585
Size: 2363 1365
BspHI T'CATG_A
Cuts at: 368 2904 368
Size: 2536 1192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 538 778 992 1294 1675 538
Size: 240 214 302 381 2591
Fragments arranged by size:
2591 381 302 240 214
BsrBI CCG'CTC
Cuts at: 50 2020 2074 3558 3723 50
Size: 1970 54 1484 165 55
Fragments arranged by size:
1970 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 431 1640 431
Size: 1209 2519
BsrFI r'CCGG_y
Cuts at: 248 1726 1907 2116 248
Size: 1478 181 209 1860
Fragments arranged by size:
1860 1478 209 181
BsrGI T'GTAC_A
Cuts at: 254 254
Size: 3728
BssSI C'ACGA_G
Cuts at: 1999 2357 1999
Size: 358 3370
BstAPI GCAn_nnn'nTGC
Cuts at: 1105 1105
Size: 3728
BstBI TT'CG_AA
Cuts at: 2089 2089
Size: 3728
BstEII G'GTnAC_C
Cuts at: 210 244 210
Size: 34 3694
BstYI r'GATC_y
Cuts at: 1 194 230 582 753 1220 1243 1578 1824
Size: 193 36 352 171 467 23 335 246
Cuts at: 1824 2836 2922 2934 3280 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 352 346 335 246 193
171 86 36 23 12
BtgI C'CryG_G
Cuts at: 215 239 497 215
Size: 24 258 3446
Fragments arranged by size:
3446 258 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 156 1678 1859 2022 156
Size: 1522 181 163 1862
Fragments arranged by size:
1862 1522 181 163
BtsI GCAGTG_nn'
Cuts at: 650 971 650
Size: 321 3407
DraI TTT'AAA
Cuts at: 727 727
Size: 3728
DrdI GACnn_nn'nnGTC
Cuts at: 517 1434 2292 517
Size: 917 858 1953
Fragments arranged by size:
1953 917 858
EaeI y'GGCC_r
Cuts at: 143 167 266 380 1313 1487 1878 1905 3250
Size: 24 99 114 933 174 391 27 1345
Cuts at: 3250 143
Size: 621
Fragments arranged by size:
1345 933 621 391 174 114 99 27
24
EagI C'GGCC_G
Cuts at: 1313 1313
Size: 3728
EarI CTCTTCn'nnn_
Cuts at: 1751 1961 1751
Size: 210 3518
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1936 2246 2392 3538 1936
Size: 310 146 1146 2126
Fragments arranged by size:
2126 1146 310 146
EcoICRI GAG'CTC
Cuts at: 740 740
Size: 3728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 1553 1985 2732 3000 727
Size: 826 432 747 268 1455
Fragments arranged by size:
1455 826 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 867 867
Size: 3728
EcoRI G'AATT_C
Cuts at: 732 732
Size: 3728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1747 1747
Size: 3728
FspI TGC'GCA
Cuts at: 1509 1509
Size: 3728
HaeII r_GCGC'y
Cuts at: 189 306 1162 1410 2432 3411 189
Size: 117 856 248 1022 979 506
Fragments arranged by size:
1022 979 856 506 248 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 122 154 631 650 663 682 1570 1600 1602
Size: 32 477 19 13 19 888 30 2
Cuts at: 1602 1632 122
Size: 30 2218
Fragments arranged by size:
2218 888 477 32 30 30 19 19
13 2
HincII GTy'rAC
Cuts at: 767 767
Size: 3728
KasI G'GCGC_C
Cuts at: 1158 1406 1158
Size: 248 3480
KpnI G_GTAC'C
Cuts at: 748 748
Size: 3728
MluI A'CGCG_T
Cuts at: 3161 3161
Size: 3728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 422 2399 2583 422
Size: 1977 184 1567
Fragments arranged by size:
1977 1567 184
MscI TGG'CCA
Cuts at: 145 169 382 1489 145
Size: 24 213 1107 2384
Fragments arranged by size:
2384 1107 213 24
MslI CAynn'nnrTG
Cuts at: 145 373 1717 1844 145
Size: 228 1344 127 2029
Fragments arranged by size:
2029 1344 228 127
MspA1I CmG'CkG
Cuts at: 839 1153 1513 2526 2771 839
Size: 314 360 1013 245 1796
Fragments arranged by size:
1796 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1909 1909
Size: 3728
NarI GG'CG_CC
Cuts at: 1159 1407 1159
Size: 248 3480
NcoI C'CATG_G
Cuts at: 239 497 239
Size: 258 3470
NgoMIV G'CCGG_C
Cuts at: 1907 1907
Size: 3728
NspI r_CATG'y
Cuts at: 781 1281 2188 781
Size: 500 907 2321
Fragments arranged by size:
2321 907 500
PciI A'CATG_T
Cuts at: 2184 2184
Size: 3728
PmeI GTTT'AAAC
Cuts at: 727 727
Size: 3728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2867 2899 2867
Size: 32 3696
PstI C_TGCA'G
Cuts at: 180 775 1460 180
Size: 595 685 2448
Fragments arranged by size:
2448 685 595
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3728
PvuII CAG'CTG
Cuts at: 1153 1513 1153
Size: 360 3368
RsrII CG'GwC_CG
Cuts at: 1923 1923
Size: 3728
SacI G_AGCT'C
Cuts at: 742 742
Size: 3728
SalI G'TCGA_C
Cuts at: 765 765
Size: 3728
SapI GCTCTTCn'nnn_
Cuts at: 1751 1961 1751
Size: 210 3518
SbfI CC_TGCA'GG
Cuts at: 775 775
Size: 3728
ScaI AGT'ACT
Cuts at: 3245 3245
Size: 3728
SfcI C'TryA_G
Cuts at: 33 176 771 1456 2449 2640 33
Size: 143 595 685 993 191 1121
Fragments arranged by size:
1121 993 685 595 191 143
SfiI GGCCn_nnn'nGGCC
Cuts at: 142 142
Size: 3728
SfoI GGC'GCC
Cuts at: 1160 1408 1160
Size: 248 3480
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
SmaI CCC'GGG
Cuts at: 750 750
Size: 3728
SmlI C'TyrA_G
Cuts at: 307 883 1798 2290 2552 307
Size: 576 915 492 262 1483
Fragments arranged by size:
1483 915 576 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3728
SphI G_CATG'C
Cuts at: 781 1281 781
Size: 500 3228
StyI C'CwwG_G
Cuts at: 239 497 862 1216 239
Size: 258 365 354 2751
Fragments arranged by size:
2751 365 354 258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 232 250 1311 1444 1903 2112 232
Size: 18 1061 133 459 209 1848
Fragments arranged by size:
1848 1061 459 209 133 18
TatI w'GTAC_w
Cuts at: 254 3243 254
Size: 2989 739
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 147 179 3245 3277 147
Size: 32 3066 32 598
Fragments arranged by size:
3066 598 32 32
Tth111I GACn'n_nGTC
Cuts at: 1525 1525
Size: 3728
XbaI T'CTAG_A
Cuts at: 477 759 477
Size: 282 3446
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1223 1223
Size: 3728
XmaI C'CCGG_G
Cuts at: 748 748
Size: 3728
ZraI GAC'GTC
Cuts at: 285 285
Size: 3728
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BpmI
Bpu10I BseRI BsgI BsiWI BsmBI BssHII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI