Restriction map for
pF1KB6905
(Circular) (Six-base) MAPSORT of: pF1KB6905.seq Check: 3971 from: 1 to: 3725
pF1KB6905 3725 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 763 763
Size: 3725
Acc65I G'GTAC_C
Cuts at: 741 741
Size: 3725
AclI AA'CG_TT
Cuts at: 3565 3565
Size: 3725
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1550 1982 2729 2997 1550
Size: 432 747 268 2278
Fragments arranged by size:
2278 747 432 268
AflII C'TTAA_G
Cuts at: 553 553
Size: 3725
AflIII A'CryG_T
Cuts at: 1077 2181 3158 1077
Size: 1104 977 1644
Fragments arranged by size:
1644 1104 977
AgeI A'CCGG_T
Cuts at: 2113 2113
Size: 3725
AlwNI CAG_nnn'CTG
Cuts at: 1030 2597 1030
Size: 1567 2158
ApaLI G'TGCA_C
Cuts at: 2495 2495
Size: 3725
ApoI r'AATT_y
Cuts at: 637 729 3078 3334 637
Size: 92 2349 256 1028
Fragments arranged by size:
2349 1028 256 92
AseI AT'TA_AT
Cuts at: 20 20
Size: 3725
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3725
AvaI C'yCGr_G
Cuts at: 745 745
Size: 3725
BamHI G'GATC_C
Cuts at: 750 750
Size: 3725
BanI G'GyrC_C
Cuts at: 741 1155 1403 1438 741
Size: 414 248 35 3028
Fragments arranged by size:
3028 414 248 35
BanII G_rGCy'C
Cuts at: 739 1769 739
Size: 1030 2695
BbeI G_GCGC'C
Cuts at: 1159 1407 1159
Size: 248 3477
BciVI GTATCCnnnnn_n'
Cuts at: 303 1623 2384 3725 303
Size: 1320 761 1341 303
Fragments arranged by size:
1341 1320 761 303
BclI T'GATC_A
Cuts at: 1245 1245
Size: 3725
BglII A'GATC_T
Cuts at: 495 1240 3277 495
Size: 745 2037 943
Fragments arranged by size:
2037 943 745
BlpI GC'TnA_GC
Cuts at: 837 837
Size: 3725
Bme1580I G_kGCm'C
Cuts at: 1350 1443 2499 1350
Size: 93 1056 2576
Fragments arranged by size:
2576 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 937 1018 1353 3497 937
Size: 81 335 2144 1165
Fragments arranged by size:
2144 1165 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2092 2092
Size: 3725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 1780 2272 2570 901
Size: 879 492 298 2056
Fragments arranged by size:
2056 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3429 3429
Size: 3725
BsaAI yAC'GTr
Cuts at: 1078 1078
Size: 3725
BsaBI GATnn'nnATC
Cuts at: 6 236 500 1239 3276 6
Size: 230 264 739 2037 455
Fragments arranged by size:
2037 739 455 264 230
BsaHI Gr'CG_yC
Cuts at: 1156 1404 2106 3602 1156
Size: 248 702 1496 1279
Fragments arranged by size:
1496 1279 702 248
BsaWI w'CCGG_w
Cuts at: 914 1435 2113 2387 2534 2945 914
Size: 521 678 274 147 411 1694
Fragments arranged by size:
1694 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1150 1908 2485 2981 1150
Size: 758 577 496 1894
Fragments arranged by size:
1894 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 119 1267 1313 2521 104
Size: 15 1148 46 1208 1308
Fragments arranged by size:
1308 1208 1148 46 15
BsiHKAI G_wGCw'C
Cuts at: 739 1517 1707 2499 739
Size: 778 190 792 1965
Fragments arranged by size:
1965 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 739 1350 1443 1517 1707 1769 2499 739
Size: 611 93 74 190 62 730 1965
Fragments arranged by size:
1965 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2945 2945
Size: 3725
BspHI T'CATG_A
Cuts at: 2901 2901
Size: 3725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 775 989 1291 1672 775
Size: 214 302 381 2828
Fragments arranged by size:
2828 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2017 2071 3555 3720 50
Size: 1967 54 1484 165 55
Fragments arranged by size:
1967 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1637 1637
Size: 3725
BsrFI r'CCGG_y
Cuts at: 118 152 1723 1904 2113 118
Size: 34 1571 181 209 1730
Fragments arranged by size:
1730 1571 209 181 34
BssSI C'ACGA_G
Cuts at: 157 1996 2354 157
Size: 1839 358 1528
Fragments arranged by size:
1839 1528 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1102 1102
Size: 3725
BstBI TT'CG_AA
Cuts at: 2086 2086
Size: 3725
BstYI r'GATC_y
Cuts at: 1 495 750 1217 1240 1575 1821 2833 2919
Size: 494 255 467 23 335 246 1012 86
Cuts at: 2919 2931 3277 1
Size: 12 346 449
Fragments arranged by size:
1012 494 467 449 346 335 255 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 113 107
Size: 6 3719
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1675 1856 2019 1675
Size: 181 163 3381
Fragments arranged by size:
3381 181 163
BtsI GCAGTG_nn'
Cuts at: 274 968 274
Size: 694 3031
DraI TTT'AAA
Cuts at: 724 724
Size: 3725
DrdI GACnn_nn'nnGTC
Cuts at: 1431 2289 1431
Size: 858 2867
EaeI y'GGCC_r
Cuts at: 110 116 138 1310 1484 1875 1902 3247 110
Size: 6 22 1172 174 391 27 1345 588
Fragments arranged by size:
1345 1172 588 391 174 27 22 6
EagI C'GGCC_G
Cuts at: 116 1310 116
Size: 1194 2531
EarI CTCTTCn'nnn_
Cuts at: 320 1748 1958 320
Size: 1428 210 2087
Fragments arranged by size:
2087 1428 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1933 2243 2389 3535 1933
Size: 310 146 1146 2123
Fragments arranged by size:
2123 1146 310 146
EcoICRI GAG'CTC
Cuts at: 737 737
Size: 3725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1550 1982 2729 2997 1550
Size: 432 747 268 2278
Fragments arranged by size:
2278 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 864 864
Size: 3725
EcoRI G'AATT_C
Cuts at: 729 729
Size: 3725
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1744 1744
Size: 3725
FspI TGC'GCA
Cuts at: 1506 1506
Size: 3725
HaeII r_GCGC'y
Cuts at: 196 1159 1407 2429 3408 196
Size: 963 248 1022 979 513
Fragments arranged by size:
1022 979 963 513 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 425 457 1567 1597 1599 1629 425
Size: 32 1110 30 2 30 2521
Fragments arranged by size:
2521 1110 32 30 30 2
HincII GTy'rAC
Cuts at: 764 764
Size: 3725
HindIII A'AGCT_T
Cuts at: 605 605
Size: 3725
KasI G'GCGC_C
Cuts at: 1155 1403 1155
Size: 248 3477
KpnI G_GTAC'C
Cuts at: 745 745
Size: 3725
MluI A'CGCG_T
Cuts at: 3158 3158
Size: 3725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 265 2396 2580 265
Size: 2131 184 1410
Fragments arranged by size:
2131 1410 184
MscI TGG'CCA
Cuts at: 1486 1486
Size: 3725
MslI CAynn'nnrTG
Cuts at: 1714 1841 1714
Size: 127 3598
MspA1I CmG'CkG
Cuts at: 115 836 1150 1510 2523 2768 115
Size: 721 314 360 1013 245 1072
Fragments arranged by size:
1072 1013 721 360 314 245
NaeI GCC'GGC
Cuts at: 120 1906 120
Size: 1786 1939
NarI GG'CG_CC
Cuts at: 1156 1404 1156
Size: 248 3477
NcoI C'CATG_G
Cuts at: 107 107
Size: 3725
NgoMIV G'CCGG_C
Cuts at: 118 1904 118
Size: 1786 1939
NspI r_CATG'y
Cuts at: 778 1278 2185 778
Size: 500 907 2318
Fragments arranged by size:
2318 907 500
PciI A'CATG_T
Cuts at: 2181 2181
Size: 3725
PmeI GTTT'AAAC
Cuts at: 724 724
Size: 3725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2864 2896 2864
Size: 32 3693
PstI C_TGCA'G
Cuts at: 772 1457 772
Size: 685 3040
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3725
PvuII CAG'CTG
Cuts at: 1150 1510 1150
Size: 360 3365
RsrII CG'GwC_CG
Cuts at: 1920 1920
Size: 3725
SacI G_AGCT'C
Cuts at: 739 739
Size: 3725
SacII CC_GC'GG
Cuts at: 116 116
Size: 3725
SalI G'TCGA_C
Cuts at: 762 762
Size: 3725
SapI GCTCTTCn'nnn_
Cuts at: 1748 1958 1748
Size: 210 3515
SbfI CC_TGCA'GG
Cuts at: 772 772
Size: 3725
ScaI AGT'ACT
Cuts at: 3242 3242
Size: 3725
SfcI C'TryA_G
Cuts at: 33 768 1453 2446 2637 33
Size: 735 685 993 191 1121
Fragments arranged by size:
1121 993 735 685 191
SfoI GGC'GCC
Cuts at: 1157 1405 1157
Size: 248 3477
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3725
SmaI CCC'GGG
Cuts at: 747 747
Size: 3725
SmlI C'TyrA_G
Cuts at: 553 880 1795 2287 2549 553
Size: 327 915 492 262 1729
Fragments arranged by size:
1729 915 492 327 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3725
SphI G_CATG'C
Cuts at: 778 1278 778
Size: 500 3225
StyI C'CwwG_G
Cuts at: 107 859 1213 107
Size: 752 354 2619
Fragments arranged by size:
2619 752 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1308 1441 1900 2109 1308
Size: 133 459 209 2924
Fragments arranged by size:
2924 459 209 133
TatI w'GTAC_w
Cuts at: 3240 3240
Size: 3725
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3242 3274 3242
Size: 32 3693
Tth111I GACn'n_nGTC
Cuts at: 1522 1522
Size: 3725
XbaI T'CTAG_A
Cuts at: 756 756
Size: 3725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1220 1220
Size: 3725
XmaI C'CCGG_G
Cuts at: 745 745
Size: 3725
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI