Restriction map for
pF1KB6903
(Circular) (Six-base) MAPSORT of: pF1KB6903.seq Check: 119 from: 1 to: 3722
pF1KB6903 3722 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 760 760
Size: 3722
Acc65I G'GTAC_C
Cuts at: 353 738 353
Size: 385 3337
AclI AA'CG_TT
Cuts at: 3562 3562
Size: 3722
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 598 1547 1979 2726 2994 178
Size: 420 949 432 747 268 906
Fragments arranged by size:
949 906 747 432 420 268
AfeI AGC'GCT
Cuts at: 583 583
Size: 3722
AflIII A'CryG_T
Cuts at: 1074 2178 3155 1074
Size: 1104 977 1641
Fragments arranged by size:
1641 1104 977
AgeI A'CCGG_T
Cuts at: 356 2110 356
Size: 1754 1968
AlwNI CAG_nnn'CTG
Cuts at: 168 1027 2594 168
Size: 859 1567 1296
Fragments arranged by size:
1567 1296 859
ApaLI G'TGCA_C
Cuts at: 2492 2492
Size: 3722
ApoI r'AATT_y
Cuts at: 260 407 726 3075 3331 260
Size: 147 319 2349 256 651
Fragments arranged by size:
2349 651 319 256 147
AseI AT'TA_AT
Cuts at: 20 20
Size: 3722
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3722
AvaI C'yCGr_G
Cuts at: 742 742
Size: 3722
BamHI G'GATC_C
Cuts at: 747 747
Size: 3722
BanI G'GyrC_C
Cuts at: 353 738 1152 1400 1435 353
Size: 385 414 248 35 2640
Fragments arranged by size:
2640 414 385 248 35
BanII G_rGCy'C
Cuts at: 736 1766 736
Size: 1030 2692
BbeI G_GCGC'C
Cuts at: 1156 1404 1156
Size: 248 3474
BbsI GAAGACnn'nnnn_
Cuts at: 707 707
Size: 3722
BbvCI CC'TCA_GC
Cuts at: 443 443
Size: 3722
BciVI GTATCCnnnnn_n'
Cuts at: 1620 2381 3722 1620
Size: 761 1341 1620
Fragments arranged by size:
1620 1341 761
BclI T'GATC_A
Cuts at: 1242 1242
Size: 3722
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3722
BglII A'GATC_T
Cuts at: 1237 3274 1237
Size: 2037 1685
BlpI GC'TnA_GC
Cuts at: 834 834
Size: 3722
Bme1580I G_kGCm'C
Cuts at: 1347 1440 2496 1347
Size: 93 1056 2573
Fragments arranged by size:
2573 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 553 652 934 1015 1350 3494 553
Size: 99 282 81 335 2144 781
Fragments arranged by size:
2144 781 335 282 99 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2089 2089
Size: 3722
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 634
Size: 3722
Bpu10I CC'TnA_GC
Cuts at: 443 443
Size: 3722
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 898 1777 2269 2567 898
Size: 879 492 298 2053
Fragments arranged by size:
2053 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3426 3426
Size: 3722
BsaAI yAC'GTr
Cuts at: 1075 1075
Size: 3722
BsaBI GATnn'nnATC
Cuts at: 6 572 1236 3273 6
Size: 566 664 2037 455
Fragments arranged by size:
2037 664 566 455
BsaHI Gr'CG_yC
Cuts at: 1153 1401 2103 3599 1153
Size: 248 702 1496 1276
Fragments arranged by size:
1496 1276 702 248
BsaWI w'CCGG_w
Cuts at: 356 911 1432 2110 2384 2531 2942 356
Size: 555 521 678 274 147 411 1136
Fragments arranged by size:
1136 678 555 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 463 493 463
Size: 30 3692
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 484 202
Size: 282 3440
BseYI C'CCAG_C
Cuts at: 1147 1905 2482 2978 1147
Size: 758 577 496 1891
Fragments arranged by size:
1891 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1264 1310 2518 104
Size: 1160 46 1208 1308
Fragments arranged by size:
1308 1208 1160 46
BsiHKAI G_wGCw'C
Cuts at: 736 1514 1704 2496 736
Size: 778 190 792 1962
Fragments arranged by size:
1962 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 736 1347 1440 1514 1704 1766 2496 736
Size: 611 93 74 190 62 730 1962
Fragments arranged by size:
1962 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2942 2942
Size: 3722
BspHI T'CATG_A
Cuts at: 2898 2898
Size: 3722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 772 986 1288 1669 772
Size: 214 302 381 2825
Fragments arranged by size:
2825 381 302 214
BsrBI CCG'CTC
Cuts at: 50 192 2014 2068 3552 3717 50
Size: 142 1822 54 1484 165 55
Fragments arranged by size:
1822 1484 165 142 55 54
BsrDI GCAATG_nn'
Cuts at: 1634 1634
Size: 3722
BsrFI r'CCGG_y
Cuts at: 356 1720 1901 2110 356
Size: 1364 181 209 1968
Fragments arranged by size:
1968 1364 209 181
BssSI C'ACGA_G
Cuts at: 1993 2351 1993
Size: 358 3364
BstAPI GCAn_nnn'nTGC
Cuts at: 168 338 1099 168
Size: 170 761 2791
Fragments arranged by size:
2791 761 170
BstBI TT'CG_AA
Cuts at: 2083 2083
Size: 3722
BstYI r'GATC_y
Cuts at: 1 747 1214 1237 1572 1818 2830 2916 2928
Size: 746 467 23 335 246 1012 86 12
Cuts at: 2928 3274 1
Size: 346 449
Fragments arranged by size:
1012 746 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3722
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 190 1672 1853 2016 190
Size: 1482 181 163 1896
Fragments arranged by size:
1896 1482 181 163
BtsI GCAGTG_nn'
Cuts at: 965 965
Size: 3722
DraI TTT'AAA
Cuts at: 721 721
Size: 3722
DrdI GACnn_nn'nnGTC
Cuts at: 1428 2286 1428
Size: 858 2864
EaeI y'GGCC_r
Cuts at: 1307 1481 1872 1899 3244 1307
Size: 174 391 27 1345 1785
Fragments arranged by size:
1785 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1307 1307
Size: 3722
EarI CTCTTCn'nnn_
Cuts at: 296 1745 1955 296
Size: 1449 210 2063
Fragments arranged by size:
2063 1449 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1930 2240 2386 3532 1930
Size: 310 146 1146 2120
Fragments arranged by size:
2120 1146 310 146
EcoICRI GAG'CTC
Cuts at: 734 734
Size: 3722
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 598 634 1547 1979 2726 2994 178
Size: 420 36 913 432 747 268 906
Fragments arranged by size:
913 906 747 432 420 268 36
EcoO109I rG'GnC_Cy
Cuts at: 654 861 654
Size: 207 3515
EcoRI G'AATT_C
Cuts at: 726 726
Size: 3722
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1741 1741
Size: 3722
FspI TGC'GCA
Cuts at: 1503 1503
Size: 3722
HaeII r_GCGC'y
Cuts at: 134 585 1156 1404 2426 3405 134
Size: 451 571 248 1022 979 451
Fragments arranged by size:
1022 979 571 451 451 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 320 352 463 482 495 514 1564 1594 1596
Size: 32 111 19 13 19 1050 30 2
Cuts at: 1596 1626 320
Size: 30 2416
Fragments arranged by size:
2416 1050 111 32 30 30 19 19
13 2
HincII GTy'rAC
Cuts at: 710 761 710
Size: 51 3671
KasI G'GCGC_C
Cuts at: 1152 1400 1152
Size: 248 3474
KpnI G_GTAC'C
Cuts at: 357 742 357
Size: 385 3337
MluI A'CGCG_T
Cuts at: 3155 3155
Size: 3722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 348 2393 2577 348
Size: 2045 184 1493
Fragments arranged by size:
2045 1493 184
MscI TGG'CCA
Cuts at: 1483 1483
Size: 3722
MslI CAynn'nnrTG
Cuts at: 379 505 1711 1838 379
Size: 126 1206 127 2263
Fragments arranged by size:
2263 1206 127 126
MspA1I CmG'CkG
Cuts at: 121 715 833 1147 1507 2520 2765 121
Size: 594 118 314 360 1013 245 1078
Fragments arranged by size:
1078 1013 594 360 314 245 118
NaeI GCC'GGC
Cuts at: 1903 1903
Size: 3722
NarI GG'CG_CC
Cuts at: 1153 1401 1153
Size: 248 3474
NcoI C'CATG_G
Cuts at: 107 107
Size: 3722
NgoMIV G'CCGG_C
Cuts at: 1901 1901
Size: 3722
NspI r_CATG'y
Cuts at: 282 775 1275 2182 282
Size: 493 500 907 1822
Fragments arranged by size:
1822 907 500 493
PciI A'CATG_T
Cuts at: 2178 2178
Size: 3722
PflMI CCAn_nnn'nTGG
Cuts at: 517 517
Size: 3722
PfoI T'CCnGG_A
Cuts at: 430 430
Size: 3722
PmeI GTTT'AAAC
Cuts at: 721 721
Size: 3722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2861 2893 2861
Size: 32 3690
PpuMI rG'GwC_Cy
Cuts at: 654 654
Size: 3722
PstI C_TGCA'G
Cuts at: 769 1454 769
Size: 685 3037
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3722
PvuII CAG'CTG
Cuts at: 715 1147 1507 715
Size: 432 360 2930
Fragments arranged by size:
2930 432 360
RsrII CG'GwC_CG
Cuts at: 1917 1917
Size: 3722
SacI G_AGCT'C
Cuts at: 736 736
Size: 3722
SalI G'TCGA_C
Cuts at: 759 759
Size: 3722
SapI GCTCTTCn'nnn_
Cuts at: 1745 1955 1745
Size: 210 3512
SbfI CC_TGCA'GG
Cuts at: 769 769
Size: 3722
ScaI AGT'ACT
Cuts at: 3239 3239
Size: 3722
SfcI C'TryA_G
Cuts at: 33 765 1450 2443 2634 33
Size: 732 685 993 191 1121
Fragments arranged by size:
1121 993 732 685 191
SfoI GGC'GCC
Cuts at: 1154 1402 1154
Size: 248 3474
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3722
SmaI CCC'GGG
Cuts at: 744 744
Size: 3722
SmlI C'TyrA_G
Cuts at: 877 1792 2284 2546 877
Size: 915 492 262 2053
Fragments arranged by size:
2053 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3722
SphI G_CATG'C
Cuts at: 282 775 1275 282
Size: 493 500 2729
Fragments arranged by size:
2729 500 493
StuI AGG'CCT
Cuts at: 475 515 475
Size: 40 3682
StyI C'CwwG_G
Cuts at: 107 516 856 1210 107
Size: 409 340 354 2619
Fragments arranged by size:
2619 409 354 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1305 1438 1897 2106 1305
Size: 133 459 209 2921
Fragments arranged by size:
2921 459 209 133
TatI w'GTAC_w
Cuts at: 3237 3237
Size: 3722
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3239 3271 3239
Size: 32 3690
Tth111I GACn'n_nGTC
Cuts at: 1519 1519
Size: 3722
XbaI T'CTAG_A
Cuts at: 753 753
Size: 3722
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1217 1217
Size: 3722
XmaI C'CCGG_G
Cuts at: 742 742
Size: 3722
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI