Restriction map for
pF1KB6901
(Circular) (Six-base) MAPSORT of: pF1KB6901.seq Check: 8571 from: 1 to: 3722
pF1KB6901 3722 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AatII G_ACGT'C
Cuts at: 323 323
Size: 3722
AccI GT'mk_AC
Cuts at: 760 760
Size: 3722
Acc65I G'GTAC_C
Cuts at: 738 738
Size: 3722
AclI AA'CG_TT
Cuts at: 567 3562 567
Size: 2995 727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1547 1979 2726 2994 1547
Size: 432 747 268 2275
Fragments arranged by size:
2275 747 432 268
AflIII A'CryG_T
Cuts at: 1074 2178 3155 1074
Size: 1104 977 1641
Fragments arranged by size:
1641 1104 977
AgeI A'CCGG_T
Cuts at: 2110 2110
Size: 3722
AlwNI CAG_nnn'CTG
Cuts at: 1027 2594 1027
Size: 1567 2155
ApaLI G'TGCA_C
Cuts at: 2492 2492
Size: 3722
ApoI r'AATT_y
Cuts at: 347 726 3075 3331 347
Size: 379 2349 256 738
Fragments arranged by size:
2349 738 379 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3722
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3722
AvaI C'yCGr_G
Cuts at: 742 742
Size: 3722
BamHI G'GATC_C
Cuts at: 747 747
Size: 3722
BanI G'GyrC_C
Cuts at: 201 738 1152 1400 1435 201
Size: 537 414 248 35 2488
Fragments arranged by size:
2488 537 414 248 35
BanII G_rGCy'C
Cuts at: 163 736 1766 163
Size: 573 1030 2119
Fragments arranged by size:
2119 1030 573
BbeI G_GCGC'C
Cuts at: 205 1156 1404 205
Size: 951 248 2523
Fragments arranged by size:
2523 951 248
BbsI GAAGACnn'nnnn_
Cuts at: 399 399
Size: 3722
BciVI GTATCCnnnnn_n'
Cuts at: 1620 2381 3722 1620
Size: 761 1341 1620
Fragments arranged by size:
1620 1341 761
BclI T'GATC_A
Cuts at: 669 1242 669
Size: 573 3149
BfrBI ATG'CAT
Cuts at: 457 457
Size: 3722
BglII A'GATC_T
Cuts at: 1237 3274 1237
Size: 2037 1685
BlpI GC'TnA_GC
Cuts at: 834 834
Size: 3722
Bme1580I G_kGCm'C
Cuts at: 1347 1440 2496 1347
Size: 93 1056 2573
Fragments arranged by size:
2573 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 934 1015 1350 3494 934
Size: 81 335 2144 1162
Fragments arranged by size:
2144 1162 335 81
BmtI G_CTAG'C
Cuts at: 707 707
Size: 3722
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2089 2089
Size: 3722
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 898 1777 2269 2567 898
Size: 879 492 298 2053
Fragments arranged by size:
2053 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3426 3426
Size: 3722
BsaAI yAC'GTr
Cuts at: 1075 1075
Size: 3722
BsaBI GATnn'nnATC
Cuts at: 6 1236 3273 6
Size: 1230 2037 455
Fragments arranged by size:
2037 1230 455
BsaHI Gr'CG_yC
Cuts at: 166 202 320 1153 1401 2103 3599 166
Size: 36 118 833 248 702 1496 289
Fragments arranged by size:
1496 833 702 289 248 118 36
BsaWI w'CCGG_w
Cuts at: 911 1432 2110 2384 2531 2942 911
Size: 521 678 274 147 411 1691
Fragments arranged by size:
1691 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 310 340 310
Size: 30 3692
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 315 454 315
Size: 139 3583
BseYI C'CCAG_C
Cuts at: 1147 1905 2482 2978 1147
Size: 758 577 496 1891
Fragments arranged by size:
1891 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 215 239 272 1264 1310 2518 104
Size: 111 24 33 992 46 1208 1308
Fragments arranged by size:
1308 1208 992 111 46 33 24
BsiHKAI G_wGCw'C
Cuts at: 736 1514 1704 2496 736
Size: 778 190 792 1962
Fragments arranged by size:
1962 792 778 190
BsmI GAATG_Cn'
Cuts at: 386 386
Size: 3722
BsmBI CGTCTCn'nnnn_
Cuts at: 327 327
Size: 3722
Bsp1286I G_dGCh'C
Cuts at: 163 736 1347 1440 1514 1704 1766 2496 163
Size: 573 611 93 74 190 62 730 1389
Fragments arranged by size:
1389 730 611 573 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2942 2942
Size: 3722
BspHI T'CATG_A
Cuts at: 2898 2898
Size: 3722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 537 772 986 1288 1669 537
Size: 235 214 302 381 2590
Fragments arranged by size:
2590 381 302 235 214
BsrBI CCG'CTC
Cuts at: 50 2014 2068 3552 3717 50
Size: 1964 54 1484 165 55
Fragments arranged by size:
1964 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1634 1634
Size: 3722
BsrFI r'CCGG_y
Cuts at: 1720 1901 2110 1720
Size: 181 209 3332
Fragments arranged by size:
3332 209 181
BsrGI T'GTAC_A
Cuts at: 353 353
Size: 3722
BssSI C'ACGA_G
Cuts at: 1993 2351 1993
Size: 358 3364
BstAPI GCAn_nnn'nTGC
Cuts at: 1099 1099
Size: 3722
BstBI TT'CG_AA
Cuts at: 2083 2083
Size: 3722
BstYI r'GATC_y
Cuts at: 1 423 747 1214 1237 1572 1818 2830 2916
Size: 422 324 467 23 335 246 1012 86
Cuts at: 2916 2928 3274 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 422 346 335 324 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 239 107
Size: 132 3590
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 198 1672 1853 2016 198
Size: 1474 181 163 1904
Fragments arranged by size:
1904 1474 181 163
BtsI GCAGTG_nn'
Cuts at: 965 965
Size: 3722
DraI TTT'AAA
Cuts at: 721 721
Size: 3722
DrdI GACnn_nn'nnGTC
Cuts at: 1428 2286 1428
Size: 858 2864
EaeI y'GGCC_r
Cuts at: 212 236 242 269 1307 1481 1872 1899 3244
Size: 24 6 27 1038 174 391 27 1345
Cuts at: 3244 212
Size: 690
Fragments arranged by size:
1345 1038 690 391 174 27 27 24
6
EagI C'GGCC_G
Cuts at: 212 236 269 1307 212
Size: 24 33 1038 2627
Fragments arranged by size:
2627 1038 33 24
EarI CTCTTCn'nnn_
Cuts at: 382 581 1745 1955 382
Size: 199 1164 210 2149
Fragments arranged by size:
2149 1164 210 199
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1930 2240 2386 3532 1930
Size: 310 146 1146 2120
Fragments arranged by size:
2120 1146 310 146
EcoICRI GAG'CTC
Cuts at: 734 734
Size: 3722
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1547 1979 2726 2994 1547
Size: 432 747 268 2275
Fragments arranged by size:
2275 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 861 861
Size: 3722
EcoRI G'AATT_C
Cuts at: 726 726
Size: 3722
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1741 1741
Size: 3722
FspI TGC'GCA
Cuts at: 1503 1503
Size: 3722
HaeII r_GCGC'y
Cuts at: 205 1156 1404 2426 3405 205
Size: 951 248 1022 979 522
Fragments arranged by size:
1022 979 951 522 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 310 342 1564 1594 1596 1626 310
Size: 32 1222 30 2 30 2406
Fragments arranged by size:
2406 1222 32 30 30 2
HincII GTy'rAC
Cuts at: 761 761
Size: 3722
KasI G'GCGC_C
Cuts at: 201 1152 1400 201
Size: 951 248 2523
Fragments arranged by size:
2523 951 248
KpnI G_GTAC'C
Cuts at: 742 742
Size: 3722
MluI A'CGCG_T
Cuts at: 3155 3155
Size: 3722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 438 2393 2577 438
Size: 1955 184 1583
Fragments arranged by size:
1955 1583 184
MscI TGG'CCA
Cuts at: 1483 1483
Size: 3722
MslI CAynn'nnrTG
Cuts at: 1711 1838 1711
Size: 127 3595
MspA1I CmG'CkG
Cuts at: 153 217 241 265 833 1147 1507 2520 2765
Size: 64 24 24 568 314 360 1013 245
Cuts at: 2765 153
Size: 1110
Fragments arranged by size:
1110 1013 568 360 314 245 64 24
24
NaeI GCC'GGC
Cuts at: 1903 1903
Size: 3722
NarI GG'CG_CC
Cuts at: 202 1153 1401 202
Size: 951 248 2523
Fragments arranged by size:
2523 951 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3722
NgoMIV G'CCGG_C
Cuts at: 1901 1901
Size: 3722
NheI G'CTAG_C
Cuts at: 703 703
Size: 3722
NotI GC'GGCC_GC
Cuts at: 236 269 236
Size: 33 3689
NsiI A_TGCA'T
Cuts at: 459 459
Size: 3722
NspI r_CATG'y
Cuts at: 457 775 1275 2182 457
Size: 318 500 907 1997
Fragments arranged by size:
1997 907 500 318
PciI A'CATG_T
Cuts at: 2178 2178
Size: 3722
PmeI GTTT'AAAC
Cuts at: 721 721
Size: 3722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2861 2893 2861
Size: 32 3690
PstI C_TGCA'G
Cuts at: 501 769 1454 501
Size: 268 685 2769
Fragments arranged by size:
2769 685 268
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3722
PvuII CAG'CTG
Cuts at: 1147 1507 1147
Size: 360 3362
RsrII CG'GwC_CG
Cuts at: 1917 1917
Size: 3722
SacI G_AGCT'C
Cuts at: 736 736
Size: 3722
SacII CC_GC'GG
Cuts at: 242 242
Size: 3722
SalI G'TCGA_C
Cuts at: 759 759
Size: 3722
SapI GCTCTTCn'nnn_
Cuts at: 1745 1955 1745
Size: 210 3512
SbfI CC_TGCA'GG
Cuts at: 769 769
Size: 3722
ScaI AGT'ACT
Cuts at: 3239 3239
Size: 3722
SfcI C'TryA_G
Cuts at: 33 497 765 1450 2443 2634 33
Size: 464 268 685 993 191 1121
Fragments arranged by size:
1121 993 685 464 268 191
SfoI GGC'GCC
Cuts at: 203 1154 1402 203
Size: 951 248 2523
Fragments arranged by size:
2523 951 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3722
SmaI CCC'GGG
Cuts at: 744 744
Size: 3722
SmlI C'TyrA_G
Cuts at: 877 1792 2284 2546 877
Size: 915 492 262 2053
Fragments arranged by size:
2053 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3722
SphI G_CATG'C
Cuts at: 775 1275 775
Size: 500 3222
SspI AAT'ATT
Cuts at: 617 617
Size: 3722
StyI C'CwwG_G
Cuts at: 107 856 1210 107
Size: 749 354 2619
Fragments arranged by size:
2619 749 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1305 1438 1897 2106 1305
Size: 133 459 209 2921
Fragments arranged by size:
2921 459 209 133
TatI w'GTAC_w
Cuts at: 353 433 3237 353
Size: 80 2804 838
Fragments arranged by size:
2804 838 80
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 546 578 3239 3271 546
Size: 32 2661 32 997
Fragments arranged by size:
2661 997 32 32
Tth111I GACn'n_nGTC
Cuts at: 1519 1519
Size: 3722
XbaI T'CTAG_A
Cuts at: 753 753
Size: 3722
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1217 1217
Size: 3722
XmaI C'CCGG_G
Cuts at: 742 742
Size: 3722
XmnI GAAnn'nnTTC
Cuts at: 347 347
Size: 3722
ZraI GAC'GTC
Cuts at: 321 321
Size: 3722
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NotI NsiI NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BpmI
Bpu10I BsgI BsiWI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NruI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI