Restriction map for
pF1KB6896
(Circular) (Six-base) MAPSORT of: pF1KB6896.seq Check: 7942 from: 1 to: 3719
pF1KB6896 3719 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 148 148
Size: 3719
AccI GT'mk_AC
Cuts at: 757 757
Size: 3719
Acc65I G'GTAC_C
Cuts at: 735 735
Size: 3719
AclI AA'CG_TT
Cuts at: 3559 3559
Size: 3719
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 643 1544 1976 2723 2991 376
Size: 267 901 432 747 268 1104
Fragments arranged by size:
1104 901 747 432 268 267
AflIII A'CryG_T
Cuts at: 169 583 1071 2175 3152 169
Size: 414 488 1104 977 736
Fragments arranged by size:
1104 977 736 488 414
AgeI A'CCGG_T
Cuts at: 2107 2107
Size: 3719
AlwNI CAG_nnn'CTG
Cuts at: 1024 2591 1024
Size: 1567 2152
ApaLI G'TGCA_C
Cuts at: 2489 2489
Size: 3719
ApoI r'AATT_y
Cuts at: 723 3072 3328 723
Size: 2349 256 1114
Fragments arranged by size:
2349 1114 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3719
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3719
AvaI C'yCGr_G
Cuts at: 739 739
Size: 3719
BamHI G'GATC_C
Cuts at: 744 744
Size: 3719
BanI G'GyrC_C
Cuts at: 735 1149 1397 1432 735
Size: 414 248 35 3022
Fragments arranged by size:
3022 414 248 35
BanII G_rGCy'C
Cuts at: 704 733 1763 704
Size: 29 1030 2660
Fragments arranged by size:
2660 1030 29
BbeI G_GCGC'C
Cuts at: 1153 1401 1153
Size: 248 3471
BbsI GAAGACnn'nnnn_
Cuts at: 364 586 364
Size: 222 3497
BbvCI CC'TCA_GC
Cuts at: 660 660
Size: 3719
BciVI GTATCCnnnnn_n'
Cuts at: 1617 2378 3719 1617
Size: 761 1341 1617
Fragments arranged by size:
1617 1341 761
BclI T'GATC_A
Cuts at: 1239 1239
Size: 3719
BglI GCCn_nnn'nGGC
Cuts at: 445 445
Size: 3719
BglII A'GATC_T
Cuts at: 455 1234 3271 455
Size: 779 2037 903
Fragments arranged by size:
2037 903 779
BlpI GC'TnA_GC
Cuts at: 316 831 316
Size: 515 3204
Bme1580I G_kGCm'C
Cuts at: 450 1344 1437 2493 450
Size: 894 93 1056 1676
Fragments arranged by size:
1676 1056 894 93
BmgBI CAC'GTC
Cuts at: 172 172
Size: 3719
BmrI ACTGGGnnnn_n'
Cuts at: 224 931 1012 1347 3491 224
Size: 707 81 335 2144 452
Fragments arranged by size:
2144 707 452 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 621 2086 621
Size: 1465 2254
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 403 426 166
Size: 237 23 3459
Fragments arranged by size:
3459 237 23
Bpu10I CC'TnA_GC
Cuts at: 572 660 572
Size: 88 3631
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 895 1774 2266 2564 895
Size: 879 492 298 2050
Fragments arranged by size:
2050 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 505 3423 505
Size: 2918 801
BsaAI yAC'GTr
Cuts at: 586 1072 586
Size: 486 3233
BsaBI GATnn'nnATC
Cuts at: 6 1233 3270 6
Size: 1227 2037 455
Fragments arranged by size:
2037 1227 455
BsaHI Gr'CG_yC
Cuts at: 1150 1398 2100 3596 1150
Size: 248 702 1496 1273
Fragments arranged by size:
1496 1273 702 248
BsaWI w'CCGG_w
Cuts at: 908 1429 2107 2381 2528 2939 908
Size: 521 678 274 147 411 1688
Fragments arranged by size:
1688 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 388 418 478 388
Size: 30 60 3629
Fragments arranged by size:
3629 60 30
BseYI C'CCAG_C
Cuts at: 201 285 1144 1902 2479 2975 201
Size: 84 859 758 577 496 945
Fragments arranged by size:
945 859 758 577 496 84
BsiEI CG_ry'CG
Cuts at: 104 1261 1307 2515 104
Size: 1157 46 1208 1308
Fragments arranged by size:
1308 1208 1157 46
BsiHKAI G_wGCw'C
Cuts at: 733 1511 1701 2493 733
Size: 778 190 792 1959
Fragments arranged by size:
1959 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 450 704 733 1344 1437 1511 1701 1763 2493
Size: 254 29 611 93 74 190 62 730
Cuts at: 2493 450
Size: 1676
Fragments arranged by size:
1676 730 611 254 190 93 74 62
29
BspEI T'CCGG_A
Cuts at: 2939 2939
Size: 3719
BspHI T'CATG_A
Cuts at: 2895 2895
Size: 3719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 148 317 545 769 983 1285 1666 148
Size: 169 228 224 214 302 381 2201
Fragments arranged by size:
2201 381 302 228 224 214 169
BsrBI CCG'CTC
Cuts at: 50 2011 2065 3549 3714 50
Size: 1961 54 1484 165 55
Fragments arranged by size:
1961 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1631 1631
Size: 3719
BsrFI r'CCGG_y
Cuts at: 1717 1898 2107 1717
Size: 181 209 3329
Fragments arranged by size:
3329 209 181
BssSI C'ACGA_G
Cuts at: 1990 2348 1990
Size: 358 3361
BstAPI GCAn_nnn'nTGC
Cuts at: 1096 1096
Size: 3719
BstBI TT'CG_AA
Cuts at: 2080 2080
Size: 3719
BstXI CCAn_nnnn'nTGG
Cuts at: 144 144
Size: 3719
BstYI r'GATC_y
Cuts at: 1 179 416 455 744 1211 1234 1569 1815
Size: 178 237 39 289 467 23 335 246
Cuts at: 1815 2827 2913 2925 3271 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 289 246 237
178 86 39 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1669 1850 2013 1669
Size: 181 163 3375
Fragments arranged by size:
3375 181 163
BtsI GCAGTG_nn'
Cuts at: 483 962 483
Size: 479 3240
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 543 578 543
Size: 35 3684
DraI TTT'AAA
Cuts at: 718 718
Size: 3719
DrdI GACnn_nn'nnGTC
Cuts at: 1425 2283 1425
Size: 858 2861
EaeI y'GGCC_r
Cuts at: 122 1304 1478 1869 1896 3241 122
Size: 1182 174 391 27 1345 600
Fragments arranged by size:
1345 1182 600 391 174 27
EagI C'GGCC_G
Cuts at: 1304 1304
Size: 3719
EarI CTCTTCn'nnn_
Cuts at: 1742 1952 1742
Size: 210 3509
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1927 2237 2383 3529 1927
Size: 310 146 1146 2117
Fragments arranged by size:
2117 1146 310 146
EcoICRI GAG'CTC
Cuts at: 731 731
Size: 3719
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 376 403 426 643 1544 1976 2723 2991
Size: 210 27 23 217 901 432 747 268
Cuts at: 2991 166
Size: 894
Fragments arranged by size:
901 894 747 432 268 217 210 27
23
EcoO109I rG'GnC_Cy
Cuts at: 483 642 858 483
Size: 159 216 3344
Fragments arranged by size:
3344 216 159
EcoRI G'AATT_C
Cuts at: 723 723
Size: 3719
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1738 1738
Size: 3719
FspI TGC'GCA
Cuts at: 1500 1500
Size: 3719
HaeII r_GCGC'y
Cuts at: 1153 1401 2423 3402 1153
Size: 248 1022 979 1470
Fragments arranged by size:
1470 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 688 720 1561 1591 1593 1623 688
Size: 32 841 30 2 30 2784
Fragments arranged by size:
2784 841 32 30 30 2
HincII GTy'rAC
Cuts at: 498 758 498
Size: 260 3459
KasI G'GCGC_C
Cuts at: 1149 1397 1149
Size: 248 3471
KpnI G_GTAC'C
Cuts at: 739 739
Size: 3719
MluI A'CGCG_T
Cuts at: 3152 3152
Size: 3719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2390 2574 2390
Size: 184 3535
MscI TGG'CCA
Cuts at: 1480 1480
Size: 3719
MslI CAynn'nnrTG
Cuts at: 1708 1835 1708
Size: 127 3592
MspA1I CmG'CkG
Cuts at: 686 830 1144 1504 2517 2762 686
Size: 144 314 360 1013 245 1643
Fragments arranged by size:
1643 1013 360 314 245 144
NaeI GCC'GGC
Cuts at: 1900 1900
Size: 3719
NarI GG'CG_CC
Cuts at: 1150 1398 1150
Size: 248 3471
NgoMIV G'CCGG_C
Cuts at: 1898 1898
Size: 3719
NspI r_CATG'y
Cuts at: 560 772 1272 2179 560
Size: 212 500 907 2100
Fragments arranged by size:
2100 907 500 212
PciI A'CATG_T
Cuts at: 2175 2175
Size: 3719
PmeI GTTT'AAAC
Cuts at: 718 718
Size: 3719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2858 2890 2858
Size: 32 3687
PpuMI rG'GwC_Cy
Cuts at: 642 642
Size: 3719
PstI C_TGCA'G
Cuts at: 542 691 766 1451 542
Size: 149 75 685 2810
Fragments arranged by size:
2810 685 149 75
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3719
PvuII CAG'CTG
Cuts at: 686 1144 1504 686
Size: 458 360 2901
Fragments arranged by size:
2901 458 360
RsrII CG'GwC_CG
Cuts at: 1914 1914
Size: 3719
SacI G_AGCT'C
Cuts at: 733 733
Size: 3719
SalI G'TCGA_C
Cuts at: 756 756
Size: 3719
SapI GCTCTTCn'nnn_
Cuts at: 1742 1952 1742
Size: 210 3509
SbfI CC_TGCA'GG
Cuts at: 766 766
Size: 3719
ScaI AGT'ACT
Cuts at: 222 3236 222
Size: 3014 705
SfcI C'TryA_G
Cuts at: 33 538 687 762 1447 2440 2631 33
Size: 505 149 75 685 993 191 1121
Fragments arranged by size:
1121 993 685 505 191 149 75
SfoI GGC'GCC
Cuts at: 1151 1399 1151
Size: 248 3471
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3719
SmaI CCC'GGG
Cuts at: 741 741
Size: 3719
SmlI C'TyrA_G
Cuts at: 874 1789 2281 2543 874
Size: 915 492 262 2050
Fragments arranged by size:
2050 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3719
SphI G_CATG'C
Cuts at: 772 1272 772
Size: 500 3219
StyI C'CwwG_G
Cuts at: 853 1207 853
Size: 354 3365
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1302 1435 1894 2103 1302
Size: 133 459 209 2918
Fragments arranged by size:
2918 459 209 133
TatI w'GTAC_w
Cuts at: 220 3234 220
Size: 3014 705
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 3687
Tth111I GACn'n_nGTC
Cuts at: 1516 1516
Size: 3719
XbaI T'CTAG_A
Cuts at: 750 750
Size: 3719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 141 690 1214 141
Size: 549 524 2646
Fragments arranged by size:
2646 549 524
XmaI C'CCGG_G
Cuts at: 739 739
Size: 3719
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BmtI BsaXI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
BtgI ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI