Restriction map for
pF1KB6877
(Circular) (Six-base) MAPSORT of: pF1KB6877.seq Check: 2104 from: 1 to: 3653
pF1KB6877 3653 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 103 430 103
Size: 327 3326
AccI GT'mk_AC
Cuts at: 176 691 176
Size: 515 3138
Acc65I G'GTAC_C
Cuts at: 308 669 308
Size: 361 3292
AclI AA'CG_TT
Cuts at: 3493 3493
Size: 3653
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1478 1910 2657 2925 1478
Size: 432 747 268 2206
Fragments arranged by size:
2206 747 432 268
AflIII A'CryG_T
Cuts at: 1005 2109 3086 1005
Size: 1104 977 1572
Fragments arranged by size:
1572 1104 977
AgeI A'CCGG_T
Cuts at: 617 2041 617
Size: 1424 2229
AlwNI CAG_nnn'CTG
Cuts at: 422 958 2525 422
Size: 536 1567 1550
Fragments arranged by size:
1567 1550 536
ApaLI G'TGCA_C
Cuts at: 2423 2423
Size: 3653
ApoI r'AATT_y
Cuts at: 657 3006 3262 657
Size: 2349 256 1048
Fragments arranged by size:
2349 1048 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3653
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
AvaI C'yCGr_G
Cuts at: 126 673 126
Size: 547 3106
BamHI G'GATC_C
Cuts at: 516 678 516
Size: 162 3491
BanI G'GyrC_C
Cuts at: 308 669 1083 1331 1366 308
Size: 361 414 248 35 2595
Fragments arranged by size:
2595 414 361 248 35
BanII G_rGCy'C
Cuts at: 667 1697 667
Size: 1030 2623
BbeI G_GCGC'C
Cuts at: 1087 1335 1087
Size: 248 3405
BbsI GAAGACnn'nnnn_
Cuts at: 574 574
Size: 3653
BciVI GTATCCnnnnn_n'
Cuts at: 1551 2312 3653 1551
Size: 761 1341 1551
Fragments arranged by size:
1551 1341 761
BclI T'GATC_A
Cuts at: 1173 1173
Size: 3653
BglII A'GATC_T
Cuts at: 1168 3205 1168
Size: 2037 1616
BlpI GC'TnA_GC
Cuts at: 765 765
Size: 3653
Bme1580I G_kGCm'C
Cuts at: 1278 1371 2427 1278
Size: 93 1056 2504
Fragments arranged by size:
2504 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 865 946 1281 3425 865
Size: 81 335 2144 1093
Fragments arranged by size:
2144 1093 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2020 2020
Size: 3653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 472 192
Size: 280 3373
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 1708 2200 2498 829
Size: 879 492 298 1984
Fragments arranged by size:
1984 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3357 3357
Size: 3653
BsaAI yAC'GTr
Cuts at: 1006 1006
Size: 3653
BsaBI GATnn'nnATC
Cuts at: 6 1167 3204 6
Size: 1161 2037 455
Fragments arranged by size:
2037 1161 455
BsaHI Gr'CG_yC
Cuts at: 1084 1332 2034 3530 1084
Size: 248 702 1496 1207
Fragments arranged by size:
1496 1207 702 248
BsaWI w'CCGG_w
Cuts at: 617 842 1363 2041 2315 2462 2873 617
Size: 225 521 678 274 147 411 1397
Fragments arranged by size:
1397 678 521 411 274 225 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 148 178 190 220 148
Size: 30 12 30 3581
Fragments arranged by size:
3581 30 30 12
BseYI C'CCAG_C
Cuts at: 1078 1836 2413 2909 1078
Size: 758 577 496 1822
Fragments arranged by size:
1822 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 439
Size: 3653
BsiEI CG_ry'CG
Cuts at: 104 1195 1241 2449 104
Size: 1091 46 1208 1308
Fragments arranged by size:
1308 1208 1091 46
BsiHKAI G_wGCw'C
Cuts at: 667 1445 1635 2427 667
Size: 778 190 792 1893
Fragments arranged by size:
1893 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 667 1278 1371 1445 1635 1697 2427 667
Size: 611 93 74 190 62 730 1893
Fragments arranged by size:
1893 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2873 2873
Size: 3653
BspHI T'CATG_A
Cuts at: 2829 2829
Size: 3653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 103 430 566 703 917 1219 1600 103
Size: 327 136 137 214 302 381 2156
Fragments arranged by size:
2156 381 327 302 214 137 136
BsrBI CCG'CTC
Cuts at: 50 1945 1999 3483 3648 50
Size: 1895 54 1484 165 55
Fragments arranged by size:
1895 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 585 1565 585
Size: 980 2673
BsrFI r'CCGG_y
Cuts at: 617 1651 1832 2041 617
Size: 1034 181 209 2229
Fragments arranged by size:
2229 1034 209 181
BssSI C'ACGA_G
Cuts at: 390 511 1924 2282 390
Size: 121 1413 358 1761
Fragments arranged by size:
1761 1413 358 121
BstAPI GCAn_nnn'nTGC
Cuts at: 1030 1030
Size: 3653
BstBI TT'CG_AA
Cuts at: 2014 2014
Size: 3653
BstEII G'GTnAC_C
Cuts at: 469 469
Size: 3653
BstXI CCAn_nnnn'nTGG
Cuts at: 348 348
Size: 3653
BstYI r'GATC_y
Cuts at: 1 516 678 1145 1168 1503 1749 2761 2847
Size: 515 162 467 23 335 246 1012 86
Cuts at: 2847 2859 3205 1
Size: 12 346 449
Fragments arranged by size:
1012 515 467 449 346 335 246 162
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3653
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1603 1784 1947 1603
Size: 181 163 3309
Fragments arranged by size:
3309 181 163
BtsI GCAGTG_nn'
Cuts at: 896 896
Size: 3653
DraI TTT'AAA
Cuts at: 652 652
Size: 3653
DrdI GACnn_nn'nnGTC
Cuts at: 1359 2217 1359
Size: 858 2795
EaeI y'GGCC_r
Cuts at: 545 1238 1412 1803 1830 3175 545
Size: 693 174 391 27 1345 1023
Fragments arranged by size:
1345 1023 693 391 174 27
EagI C'GGCC_G
Cuts at: 1238 1238
Size: 3653
EarI CTCTTCn'nnn_
Cuts at: 1676 1886 1676
Size: 210 3443
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1861 2171 2317 3463 1861
Size: 310 146 1146 2051
Fragments arranged by size:
2051 1146 310 146
EcoICRI GAG'CTC
Cuts at: 665 665
Size: 3653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 472 1478 1910 2657 2925 192
Size: 280 1006 432 747 268 920
Fragments arranged by size:
1006 920 747 432 280 268
EcoNI CCTnn'n_nnAGG
Cuts at: 463 463
Size: 3653
EcoO109I rG'GnC_Cy
Cuts at: 135 483 603 625 792 135
Size: 348 120 22 167 2996
Fragments arranged by size:
2996 348 167 120 22
EcoRI G'AATT_C
Cuts at: 657 657
Size: 3653
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1672 1672
Size: 3653
FspI TGC'GCA
Cuts at: 1434 1434
Size: 3653
HaeII r_GCGC'y
Cuts at: 1087 1335 2357 3336 1087
Size: 248 1022 979 1404
Fragments arranged by size:
1404 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1495 1525 1527 1557 1495
Size: 30 2 30 3591
Fragments arranged by size:
3591 30 30 2
HincII GTy'rAC
Cuts at: 177 692 177
Size: 515 3138
KasI G'GCGC_C
Cuts at: 1083 1331 1083
Size: 248 3405
KpnI G_GTAC'C
Cuts at: 312 673 312
Size: 361 3292
MluI A'CGCG_T
Cuts at: 3086 3086
Size: 3653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2324 2508 2324
Size: 184 3469
MscI TGG'CCA
Cuts at: 547 1414 547
Size: 867 2786
MslI CAynn'nnrTG
Cuts at: 1642 1769 1642
Size: 127 3526
MspA1I CmG'CkG
Cuts at: 764 1078 1438 2451 2696 764
Size: 314 360 1013 245 1721
Fragments arranged by size:
1721 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1834 1834
Size: 3653
NarI GG'CG_CC
Cuts at: 1084 1332 1084
Size: 248 3405
NcoI C'CATG_G
Cuts at: 107 107
Size: 3653
NgoMIV G'CCGG_C
Cuts at: 1832 1832
Size: 3653
NspI r_CATG'y
Cuts at: 706 1206 2113 706
Size: 500 907 2246
Fragments arranged by size:
2246 907 500
PasI CC'CwG_GG
Cuts at: 465 465
Size: 3653
PciI A'CATG_T
Cuts at: 2109 2109
Size: 3653
PfoI T'CCnGG_A
Cuts at: 251 251
Size: 3653
PmeI GTTT'AAAC
Cuts at: 652 652
Size: 3653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 394 426 2792 2824 394
Size: 32 2366 32 1223
Fragments arranged by size:
2366 1223 32 32
PpuMI rG'GwC_Cy
Cuts at: 135 483 603 625 135
Size: 348 120 22 3163
Fragments arranged by size:
3163 348 120 22
PstI C_TGCA'G
Cuts at: 540 700 1385 540
Size: 160 685 2808
Fragments arranged by size:
2808 685 160
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3653
PvuII CAG'CTG
Cuts at: 1078 1438 1078
Size: 360 3293
RsrII CG'GwC_CG
Cuts at: 1848 1848
Size: 3653
SacI G_AGCT'C
Cuts at: 667 667
Size: 3653
SalI G'TCGA_C
Cuts at: 175 690 175
Size: 515 3138
SanDI GG'GwC_CC
Cuts at: 625 625
Size: 3653
SapI GCTCTTCn'nnn_
Cuts at: 1676 1886 1676
Size: 210 3443
SbfI CC_TGCA'GG
Cuts at: 700 700
Size: 3653
ScaI AGT'ACT
Cuts at: 3170 3170
Size: 3653
SexAI A'CCwGG_T
Cuts at: 479 644 479
Size: 165 3488
SfcI C'TryA_G
Cuts at: 33 189 536 696 1381 2374 2565 33
Size: 156 347 160 685 993 191 1121
Fragments arranged by size:
1121 993 685 347 191 160 156
SfoI GGC'GCC
Cuts at: 1085 1333 1085
Size: 248 3405
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
SmaI CCC'GGG
Cuts at: 128 675 128
Size: 547 3106
SmlI C'TyrA_G
Cuts at: 808 1723 2215 2477 808
Size: 915 492 262 1984
Fragments arranged by size:
1984 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3653
SphI G_CATG'C
Cuts at: 706 1206 706
Size: 500 3153
StyI C'CwwG_G
Cuts at: 107 787 1141 107
Size: 680 354 2619
Fragments arranged by size:
2619 680 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1236 1369 1828 2037 1236
Size: 133 459 209 2852
Fragments arranged by size:
2852 459 209 133
TatI w'GTAC_w
Cuts at: 3168 3168
Size: 3653
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 190 222 3170 3202 190
Size: 32 2948 32 641
Fragments arranged by size:
2948 641 32 32
Tth111I GACn'n_nGTC
Cuts at: 1450 1450
Size: 3653
XbaI T'CTAG_A
Cuts at: 684 684
Size: 3653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1148 1148
Size: 3653
XmaI C'CCGG_G
Cuts at: 126 673 126
Size: 547 3106
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI