Restriction map for
pF1KB6876
(Circular) (Six-base) MAPSORT of: pF1KB6876.seq Check: 9592 from: 1 to: 3713
pF1KB6876 3713 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 751 751
Size: 3713
Acc65I G'GTAC_C
Cuts at: 200 729 200
Size: 529 3184
AclI AA'CG_TT
Cuts at: 3553 3553
Size: 3713
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 1538 1970 2717 2985 235
Size: 1303 432 747 268 963
Fragments arranged by size:
1303 963 747 432 268
AflIII A'CryG_T
Cuts at: 1065 2169 3146 1065
Size: 1104 977 1632
Fragments arranged by size:
1632 1104 977
AgeI A'CCGG_T
Cuts at: 2101 2101
Size: 3713
AlwNI CAG_nnn'CTG
Cuts at: 411 1018 2585 411
Size: 607 1567 1539
Fragments arranged by size:
1567 1539 607
ApaI G_GGCC'C
Cuts at: 132 132
Size: 3713
ApaLI G'TGCA_C
Cuts at: 2483 2483
Size: 3713
ApoI r'AATT_y
Cuts at: 619 650 717 3066 3322 619
Size: 31 67 2349 256 1010
Fragments arranged by size:
2349 1010 256 67 31
AseI AT'TA_AT
Cuts at: 20 20
Size: 3713
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3713
AvaI C'yCGr_G
Cuts at: 511 733 511
Size: 222 3491
BamHI G'GATC_C
Cuts at: 738 738
Size: 3713
BanI G'GyrC_C
Cuts at: 200 343 729 1143 1391 1426 200
Size: 143 386 414 248 35 2487
Fragments arranged by size:
2487 414 386 248 143 35
BanII G_rGCy'C
Cuts at: 132 727 1757 132
Size: 595 1030 2088
Fragments arranged by size:
2088 1030 595
BbeI G_GCGC'C
Cuts at: 1147 1395 1147
Size: 248 3465
BciVI GTATCCnnnnn_n'
Cuts at: 1611 2372 3713 1611
Size: 761 1341 1611
Fragments arranged by size:
1611 1341 761
BclI T'GATC_A
Cuts at: 503 1233 503
Size: 730 2983
BglII A'GATC_T
Cuts at: 569 1228 3265 569
Size: 659 2037 1017
Fragments arranged by size:
2037 1017 659
BlpI GC'TnA_GC
Cuts at: 825 825
Size: 3713
Bme1580I G_kGCm'C
Cuts at: 132 1338 1431 2487 132
Size: 1206 93 1056 1358
Fragments arranged by size:
1358 1206 1056 93
BmgBI CAC'GTC
Cuts at: 438 519 438
Size: 81 3632
BmrI ACTGGGnnnn_n'
Cuts at: 925 1006 1341 3485 925
Size: 81 335 2144 1153
Fragments arranged by size:
2144 1153 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 210 2080 210
Size: 1870 1843
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 433 496 169
Size: 264 63 3386
Fragments arranged by size:
3386 264 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 1768 2260 2558 889
Size: 879 492 298 2044
Fragments arranged by size:
2044 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3417 3417
Size: 3713
BsaAI yAC'GTr
Cuts at: 1066 1066
Size: 3713
BsaBI GATnn'nnATC
Cuts at: 6 1227 3264 6
Size: 1221 2037 455
Fragments arranged by size:
2037 1221 455
BsaHI Gr'CG_yC
Cuts at: 1144 1392 2094 3590 1144
Size: 248 702 1496 1267
Fragments arranged by size:
1496 1267 702 248
BsaWI w'CCGG_w
Cuts at: 902 1423 2101 2375 2522 2933 902
Size: 521 678 274 147 411 1682
Fragments arranged by size:
1682 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 175 205 175
Size: 30 3683
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 166 196 199 166
Size: 30 3 3680
Fragments arranged by size:
3680 30 3
BseYI C'CCAG_C
Cuts at: 242 305 1138 1896 2473 2969 242
Size: 63 833 758 577 496 986
Fragments arranged by size:
986 833 758 577 496 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 361
Size: 3713
BsiEI CG_ry'CG
Cuts at: 104 234 1255 1301 2509 104
Size: 130 1021 46 1208 1308
Fragments arranged by size:
1308 1208 1021 130 46
BsiHKAI G_wGCw'C
Cuts at: 196 727 1505 1695 2487 196
Size: 531 778 190 792 1422
Fragments arranged by size:
1422 792 778 531 190
Bsp1286I G_dGCh'C
Cuts at: 132 196 727 1338 1431 1505 1695 1757 2487
Size: 64 531 611 93 74 190 62 730
Cuts at: 2487 132
Size: 1358
Fragments arranged by size:
1358 730 611 531 190 93 74 64
62
BspEI T'CCGG_A
Cuts at: 2933 2933
Size: 3713
BspHI T'CATG_A
Cuts at: 2889 2889
Size: 3713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 763 977 1279 1660 763
Size: 214 302 381 2816
Fragments arranged by size:
2816 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2005 2059 3543 3708 50
Size: 1955 54 1484 165 55
Fragments arranged by size:
1955 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1625 1625
Size: 3713
BsrFI r'CCGG_y
Cuts at: 229 1711 1892 2101 229
Size: 1482 181 209 1841
Fragments arranged by size:
1841 1482 209 181
BssSI C'ACGA_G
Cuts at: 1984 2342 1984
Size: 358 3355
BstAPI GCAn_nnn'nTGC
Cuts at: 1090 1090
Size: 3713
BstBI TT'CG_AA
Cuts at: 2074 2074
Size: 3713
BstYI r'GATC_y
Cuts at: 1 143 569 738 1205 1228 1563 1809 2821
Size: 142 426 169 467 23 335 246 1012
Cuts at: 2821 2907 2919 3265 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 426 346 335 246 169
142 86 23 12
BtgI C'CryG_G
Cuts at: 107 389 107
Size: 282 3431
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 354 1663 1844 2007 354
Size: 1309 181 163 2060
Fragments arranged by size:
2060 1309 181 163
BtsI GCAGTG_nn'
Cuts at: 956 956
Size: 3713
DraI TTT'AAA
Cuts at: 712 712
Size: 3713
DrdI GACnn_nn'nnGTC
Cuts at: 1419 2277 1419
Size: 858 2855
EaeI y'GGCC_r
Cuts at: 231 638 1298 1472 1863 1890 3235 231
Size: 407 660 174 391 27 1345 709
Fragments arranged by size:
1345 709 660 407 391 174 27
EagI C'GGCC_G
Cuts at: 231 1298 231
Size: 1067 2646
EarI CTCTTCn'nnn_
Cuts at: 1736 1946 1736
Size: 210 3503
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1921 2231 2377 3523 1921
Size: 310 146 1146 2111
Fragments arranged by size:
2111 1146 310 146
EcoICRI GAG'CTC
Cuts at: 725 725
Size: 3713
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 235 433 496 1538 1970 2717 2985 169
Size: 66 198 63 1042 432 747 268 897
Fragments arranged by size:
1042 897 747 432 268 198 66 63
EcoNI CCTnn'n_nnAGG
Cuts at: 136 136
Size: 3713
EcoO109I rG'GnC_Cy
Cuts at: 128 129 852 128
Size: 1 723 2989
Fragments arranged by size:
2989 723 1
EcoRI G'AATT_C
Cuts at: 619 717 619
Size: 98 3615
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1732 1732
Size: 3713
FspI TGC'GCA
Cuts at: 1494 1494
Size: 3713
HaeII r_GCGC'y
Cuts at: 1147 1395 2417 3396 1147
Size: 248 1022 979 1464
Fragments arranged by size:
1464 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1555 1585 1587 1617 1555
Size: 30 2 30 3651
Fragments arranged by size:
3651 30 30 2
HincII GTy'rAC
Cuts at: 752 752
Size: 3713
KasI G'GCGC_C
Cuts at: 1143 1391 1143
Size: 248 3465
KpnI G_GTAC'C
Cuts at: 204 733 204
Size: 529 3184
MluI A'CGCG_T
Cuts at: 3146 3146
Size: 3713
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 356 2384 2568 356
Size: 2028 184 1501
Fragments arranged by size:
2028 1501 184
MscI TGG'CCA
Cuts at: 640 1474 640
Size: 834 2879
MslI CAynn'nnrTG
Cuts at: 1702 1829 1702
Size: 127 3586
MspA1I CmG'CkG
Cuts at: 162 391 824 1138 1498 2511 2756 162
Size: 229 433 314 360 1013 245 1119
Fragments arranged by size:
1119 1013 433 360 314 245 229
NaeI GCC'GGC
Cuts at: 1894 1894
Size: 3713
NarI GG'CG_CC
Cuts at: 1144 1392 1144
Size: 248 3465
NcoI C'CATG_G
Cuts at: 107 107
Size: 3713
NgoMIV G'CCGG_C
Cuts at: 1892 1892
Size: 3713
NspI r_CATG'y
Cuts at: 313 766 1266 2173 313
Size: 453 500 907 1853
Fragments arranged by size:
1853 907 500 453
PciI A'CATG_T
Cuts at: 2169 2169
Size: 3713
PflMI CCAn_nnn'nTGG
Cuts at: 411 411
Size: 3713
PfoI T'CCnGG_A
Cuts at: 146 146
Size: 3713
PmeI GTTT'AAAC
Cuts at: 712 712
Size: 3713
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2852 2884 2852
Size: 32 3681
PspOMI G'GGCC_C
Cuts at: 128 128
Size: 3713
PstI C_TGCA'G
Cuts at: 161 760 1445 161
Size: 599 685 2429
Fragments arranged by size:
2429 685 599
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3713
PvuII CAG'CTG
Cuts at: 162 1138 1498 162
Size: 976 360 2377
Fragments arranged by size:
2377 976 360
RsrII CG'GwC_CG
Cuts at: 1908 1908
Size: 3713
SacI G_AGCT'C
Cuts at: 727 727
Size: 3713
SacII CC_GC'GG
Cuts at: 392 392
Size: 3713
SalI G'TCGA_C
Cuts at: 750 750
Size: 3713
SapI GCTCTTCn'nnn_
Cuts at: 1736 1946 1736
Size: 210 3503
SbfI CC_TGCA'GG
Cuts at: 760 760
Size: 3713
ScaI AGT'ACT
Cuts at: 580 3230 580
Size: 2650 1063
SfcI C'TryA_G
Cuts at: 33 157 756 1441 2434 2625 33
Size: 124 599 685 993 191 1121
Fragments arranged by size:
1121 993 685 599 191 124
SfoI GGC'GCC
Cuts at: 1145 1393 1145
Size: 248 3465
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3713
SmaI CCC'GGG
Cuts at: 735 735
Size: 3713
SmlI C'TyrA_G
Cuts at: 868 1783 2275 2537 868
Size: 915 492 262 2044
Fragments arranged by size:
2044 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3713
SphI G_CATG'C
Cuts at: 766 1266 766
Size: 500 3213
StuI AGG'CCT
Cuts at: 298 298
Size: 3713
StyI C'CwwG_G
Cuts at: 107 137 293 847 1201 107
Size: 30 156 554 354 2619
Fragments arranged by size:
2619 554 354 156 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 256 1296 1429 1888 2097 256
Size: 1040 133 459 209 1872
Fragments arranged by size:
1872 1040 459 209 133
TatI w'GTAC_w
Cuts at: 578 3228 578
Size: 2650 1063
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 175 207 3230 3262 175
Size: 32 3023 32 626
Fragments arranged by size:
3023 626 32 32
Tth111I GACn'n_nGTC
Cuts at: 1510 1510
Size: 3713
XbaI T'CTAG_A
Cuts at: 744 744
Size: 3713
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 144 408 1208 144
Size: 264 800 2649
Fragments arranged by size:
2649 800 264
XmaI C'CCGG_G
Cuts at: 733 733
Size: 3713
XmnI GAAnn'nnTTC
Cuts at: 619 619
Size: 3713
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PpuMI PshAI PsiI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI