Restriction map for
pF1KB6875
(Circular) (Six-base) MAPSORT of: pF1KB6875.seq Check: 1829 from: 1 to: 3590
pF1KB6875 3590 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 628 628
Size: 3590
Acc65I G'GTAC_C
Cuts at: 273 606 273
Size: 333 3257
AclI AA'CG_TT
Cuts at: 3430 3430
Size: 3590
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 1415 1847 2594 2862 150
Size: 1265 432 747 268 878
Fragments arranged by size:
1265 878 747 432 268
AfeI AGC'GCT
Cuts at: 418 418
Size: 3590
AflIII A'CryG_T
Cuts at: 942 2046 3023 942
Size: 1104 977 1509
Fragments arranged by size:
1509 1104 977
AgeI A'CCGG_T
Cuts at: 1978 1978
Size: 3590
AhdI GACnn_n'nnGTC
Cuts at: 244 244
Size: 3590
AlwNI CAG_nnn'CTG
Cuts at: 173 895 2462 173
Size: 722 1567 1301
Fragments arranged by size:
1567 1301 722
ApaLI G'TGCA_C
Cuts at: 2360 2360
Size: 3590
ApoI r'AATT_y
Cuts at: 562 594 2943 3199 562
Size: 32 2349 256 953
Fragments arranged by size:
2349 953 256 32
AseI AT'TA_AT
Cuts at: 20 20
Size: 3590
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3590
AvaI C'yCGr_G
Cuts at: 610 610
Size: 3590
BamHI G'GATC_C
Cuts at: 615 615
Size: 3590
BanI G'GyrC_C
Cuts at: 273 606 1020 1268 1303 273
Size: 333 414 248 35 2560
Fragments arranged by size:
2560 414 333 248 35
BanII G_rGCy'C
Cuts at: 604 1634 604
Size: 1030 2560
BbeI G_GCGC'C
Cuts at: 1024 1272 1024
Size: 248 3342
BbsI GAAGACnn'nnnn_
Cuts at: 196 196
Size: 3590
BciVI GTATCCnnnnn_n'
Cuts at: 220 1488 2249 3590 220
Size: 1268 761 1341 220
Fragments arranged by size:
1341 1268 761 220
BclI T'GATC_A
Cuts at: 1110 1110
Size: 3590
BglII A'GATC_T
Cuts at: 1105 3142 1105
Size: 2037 1553
BlpI GC'TnA_GC
Cuts at: 702 702
Size: 3590
Bme1580I G_kGCm'C
Cuts at: 1215 1308 2364 1215
Size: 93 1056 2441
Fragments arranged by size:
2441 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 802 883 1218 3362 802
Size: 81 335 2144 1030
Fragments arranged by size:
2144 1030 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1957 1957
Size: 3590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 388 346
Size: 42 3548
Bpu10I CC'TnA_GC
Cuts at: 391 391
Size: 3590
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 1645 2137 2435 766
Size: 879 492 298 1921
Fragments arranged by size:
1921 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 346 3294 346
Size: 2948 642
BsaAI yAC'GTr
Cuts at: 943 943
Size: 3590
BsaBI GATnn'nnATC
Cuts at: 6 1104 3141 6
Size: 1098 2037 455
Fragments arranged by size:
2037 1098 455
BsaHI Gr'CG_yC
Cuts at: 548 1021 1269 1971 3467 548
Size: 473 248 702 1496 671
Fragments arranged by size:
1496 702 671 473 248
BsaWI w'CCGG_w
Cuts at: 779 1300 1978 2252 2399 2810 779
Size: 521 678 274 147 411 1559
Fragments arranged by size:
1559 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1015 1773 2350 2846 1015
Size: 758 577 496 1759
Fragments arranged by size:
1759 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 477 477
Size: 3590
BsiEI CG_ry'CG
Cuts at: 104 1132 1178 2386 104
Size: 1028 46 1208 1308
Fragments arranged by size:
1308 1208 1028 46
BsiHKAI G_wGCw'C
Cuts at: 604 1382 1572 2364 604
Size: 778 190 792 1830
Fragments arranged by size:
1830 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 112 112
Size: 3590
Bsp1286I G_dGCh'C
Cuts at: 604 1215 1308 1382 1572 1634 2364 604
Size: 611 93 74 190 62 730 1830
Fragments arranged by size:
1830 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2810 2810
Size: 3590
BspHI T'CATG_A
Cuts at: 2766 2766
Size: 3590
BspMI ACCTGCnnnn'nnnn_
Cuts at: 640 854 1156 1537 640
Size: 214 302 381 2693
Fragments arranged by size:
2693 381 302 214
BsrBI CCG'CTC
Cuts at: 50 434 1882 1936 3420 3585 50
Size: 384 1448 54 1484 165 55
Fragments arranged by size:
1484 1448 384 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1502 1502
Size: 3590
BsrFI r'CCGG_y
Cuts at: 1588 1769 1978 1588
Size: 181 209 3200
Fragments arranged by size:
3200 209 181
BssSI C'ACGA_G
Cuts at: 1861 2219 1861
Size: 358 3232
BstAPI GCAn_nnn'nTGC
Cuts at: 967 967
Size: 3590
BstBI TT'CG_AA
Cuts at: 1951 1951
Size: 3590
BstEII G'GTnAC_C
Cuts at: 555 555
Size: 3590
BstYI r'GATC_y
Cuts at: 1 615 1082 1105 1440 1686 2698 2784 2796
Size: 614 467 23 335 246 1012 86 12
Cuts at: 2796 3142 1
Size: 346 449
Fragments arranged by size:
1012 614 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 309 107
Size: 202 3388
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 514 1540 1721 1884 514
Size: 1026 181 163 2220
Fragments arranged by size:
2220 1026 181 163
BtsI GCAGTG_nn'
Cuts at: 833 833
Size: 3590
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 441 476 441
Size: 35 3555
DraI TTT'AAA
Cuts at: 589 589
Size: 3590
DrdI GACnn_nn'nnGTC
Cuts at: 517 547 1296 2154 517
Size: 30 749 858 1953
Fragments arranged by size:
1953 858 749 30
EaeI y'GGCC_r
Cuts at: 1175 1349 1740 1767 3112 1175
Size: 174 391 27 1345 1653
Fragments arranged by size:
1653 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1175 1175
Size: 3590
EarI CTCTTCn'nnn_
Cuts at: 1613 1823 1613
Size: 210 3380
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1798 2108 2254 3400 1798
Size: 310 146 1146 1988
Fragments arranged by size:
1988 1146 310 146
EcoICRI GAG'CTC
Cuts at: 602 602
Size: 3590
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 346 388 1415 1847 2594 2862 150
Size: 196 42 1027 432 747 268 878
Fragments arranged by size:
1027 878 747 432 268 196 42
EcoNI CCTnn'n_nnAGG
Cuts at: 542 542
Size: 3590
EcoO109I rG'GnC_Cy
Cuts at: 729 729
Size: 3590
EcoRI G'AATT_C
Cuts at: 594 594
Size: 3590
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1609 1609
Size: 3590
FspI TGC'GCA
Cuts at: 1371 1371
Size: 3590
HaeII r_GCGC'y
Cuts at: 420 1024 1272 2294 3273 420
Size: 604 248 1022 979 737
Fragments arranged by size:
1022 979 737 604 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 183 215 230 262 1432 1462 1464 1494 183
Size: 32 15 32 1170 30 2 30 2279
Fragments arranged by size:
2279 1170 32 32 30 30 15 2
HincII GTy'rAC
Cuts at: 183 378 629 183
Size: 195 251 3144
Fragments arranged by size:
3144 251 195
HpaI GTT'AAC
Cuts at: 183 378 183
Size: 195 3395
KasI G'GCGC_C
Cuts at: 1020 1268 1020
Size: 248 3342
KpnI G_GTAC'C
Cuts at: 277 610 277
Size: 333 3257
MluI A'CGCG_T
Cuts at: 3023 3023
Size: 3590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2261 2445 2261
Size: 184 3406
MscI TGG'CCA
Cuts at: 1351 1351
Size: 3590
MslI CAynn'nnrTG
Cuts at: 499 532 1579 1706 499
Size: 33 1047 127 2383
Fragments arranged by size:
2383 1047 127 33
MspA1I CmG'CkG
Cuts at: 366 701 1015 1375 2388 2633 366
Size: 335 314 360 1013 245 1323
Fragments arranged by size:
1323 1013 360 335 314 245
NaeI GCC'GGC
Cuts at: 1771 1771
Size: 3590
NarI GG'CG_CC
Cuts at: 1021 1269 1021
Size: 248 3342
NcoI C'CATG_G
Cuts at: 107 309 107
Size: 202 3388
NgoMIV G'CCGG_C
Cuts at: 1769 1769
Size: 3590
NspI r_CATG'y
Cuts at: 643 1143 2050 643
Size: 500 907 2183
Fragments arranged by size:
2183 907 500
PciI A'CATG_T
Cuts at: 2046 2046
Size: 3590
PfoI T'CCnGG_A
Cuts at: 572 572
Size: 3590
PmeI GTTT'AAAC
Cuts at: 589 589
Size: 3590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2729 2761 2729
Size: 32 3558
PstI C_TGCA'G
Cuts at: 637 1322 637
Size: 685 2905
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3590
PvuII CAG'CTG
Cuts at: 366 1015 1375 366
Size: 649 360 2581
Fragments arranged by size:
2581 649 360
RsrII CG'GwC_CG
Cuts at: 1785 1785
Size: 3590
SacI G_AGCT'C
Cuts at: 604 604
Size: 3590
SalI G'TCGA_C
Cuts at: 627 627
Size: 3590
SapI GCTCTTCn'nnn_
Cuts at: 1613 1823 1613
Size: 210 3380
SbfI CC_TGCA'GG
Cuts at: 637 637
Size: 3590
ScaI AGT'ACT
Cuts at: 3107 3107
Size: 3590
SfcI C'TryA_G
Cuts at: 33 633 1318 2311 2502 33
Size: 600 685 993 191 1121
Fragments arranged by size:
1121 993 685 600 191
SfoI GGC'GCC
Cuts at: 1022 1270 1022
Size: 248 3342
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3590
SmaI CCC'GGG
Cuts at: 612 612
Size: 3590
SmlI C'TyrA_G
Cuts at: 745 1660 2152 2414 745
Size: 915 492 262 1921
Fragments arranged by size:
1921 915 492 262
SpeI A'CTAG_T
Cuts at: 67 216 67
Size: 149 3441
SphI G_CATG'C
Cuts at: 643 1143 643
Size: 500 3090
StyI C'CwwG_G
Cuts at: 107 309 724 1078 107
Size: 202 415 354 2619
Fragments arranged by size:
2619 415 354 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1173 1306 1765 1974 1173
Size: 133 459 209 2789
Fragments arranged by size:
2789 459 209 133
TatI w'GTAC_w
Cuts at: 3105 3105
Size: 3590
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3107 3139 3107
Size: 32 3558
Tth111I GACn'n_nGTC
Cuts at: 1387 1387
Size: 3590
XbaI T'CTAG_A
Cuts at: 621 621
Size: 3590
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 363 1085 363
Size: 722 2868
XmaI C'CCGG_G
Cuts at: 610 610
Size: 3590
XmnI GAAnn'nnTTC
Cuts at: 193 193
Size: 3590
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BsaXI BseRI BsiWI BsmI BsrGI BssHII BstXI BstZ17I
Bsu36I ClaI DraIII EcoRV FseI FspAI HindIII MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI