Restriction map for
pF1KB6874
(Circular) (Six-base) MAPSORT of: pF1KB6874.seq Check: 753 from: 1 to: 3707
pF1KB6874 3707 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 745 745
Size: 3707
Acc65I G'GTAC_C
Cuts at: 723 723
Size: 3707
AclI AA'CG_TT
Cuts at: 3547 3547
Size: 3707
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 356 1532 1964 2711 2979 356
Size: 1176 432 747 268 1084
Fragments arranged by size:
1176 1084 747 432 268
AflIII A'CryG_T
Cuts at: 1059 2163 3140 1059
Size: 1104 977 1626
Fragments arranged by size:
1626 1104 977
AgeI A'CCGG_T
Cuts at: 2095 2095
Size: 3707
AlwNI CAG_nnn'CTG
Cuts at: 391 1012 2579 391
Size: 621 1567 1519
Fragments arranged by size:
1567 1519 621
ApaI G_GGCC'C
Cuts at: 260 260
Size: 3707
ApaLI G'TGCA_C
Cuts at: 2477 2477
Size: 3707
ApoI r'AATT_y
Cuts at: 711 3060 3316 711
Size: 2349 256 1102
Fragments arranged by size:
2349 1102 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3707
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3707
AvaI C'yCGr_G
Cuts at: 259 727 259
Size: 468 3239
BamHI G'GATC_C
Cuts at: 732 732
Size: 3707
BanI G'GyrC_C
Cuts at: 354 723 1137 1385 1420 354
Size: 369 414 248 35 2641
Fragments arranged by size:
2641 414 369 248 35
BanII G_rGCy'C
Cuts at: 231 260 721 1751 231
Size: 29 461 1030 2187
Fragments arranged by size:
2187 1030 461 29
BbeI G_GCGC'C
Cuts at: 1141 1389 1141
Size: 248 3459
BbsI GAAGACnn'nnnn_
Cuts at: 323 585 323
Size: 262 3445
BciVI GTATCCnnnnn_n'
Cuts at: 1605 2366 3707 1605
Size: 761 1341 1605
Fragments arranged by size:
1605 1341 761
BclI T'GATC_A
Cuts at: 1227 1227
Size: 3707
BglII A'GATC_T
Cuts at: 300 1222 3259 300
Size: 922 2037 748
Fragments arranged by size:
2037 922 748
BlpI GC'TnA_GC
Cuts at: 819 819
Size: 3707
Bme1580I G_kGCm'C
Cuts at: 260 1332 1425 2481 260
Size: 1072 93 1056 1486
Fragments arranged by size:
1486 1072 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 919 1000 1335 3479 919
Size: 81 335 2144 1147
Fragments arranged by size:
2144 1147 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2074 2074
Size: 3707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 317
Size: 3707
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 1762 2254 2552 883
Size: 879 492 298 2038
Fragments arranged by size:
2038 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3411 3411
Size: 3707
BsaAI yAC'GTr
Cuts at: 1060 1060
Size: 3707
BsaBI GATnn'nnATC
Cuts at: 6 1221 3258 6
Size: 1215 2037 455
Fragments arranged by size:
2037 1215 455
BsaHI Gr'CG_yC
Cuts at: 1138 1386 2088 3584 1138
Size: 248 702 1496 1261
Fragments arranged by size:
1496 1261 702 248
BsaWI w'CCGG_w
Cuts at: 223 896 1417 2095 2369 2516 2927 223
Size: 673 521 678 274 147 411 1003
Fragments arranged by size:
1003 678 673 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 442 442
Size: 3707
BseYI C'CCAG_C
Cuts at: 1132 1890 2467 2963 1132
Size: 758 577 496 1876
Fragments arranged by size:
1876 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 532
Size: 3707
BsiEI CG_ry'CG
Cuts at: 104 1249 1295 2503 104
Size: 1145 46 1208 1308
Fragments arranged by size:
1308 1208 1145 46
BsiHKAI G_wGCw'C
Cuts at: 721 1499 1689 2481 721
Size: 778 190 792 1947
Fragments arranged by size:
1947 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 231 260 721 1332 1425 1499 1689 1751 2481
Size: 29 461 611 93 74 190 62 730
Cuts at: 2481 231
Size: 1457
Fragments arranged by size:
1457 730 611 461 190 93 74 62
29
BspEI T'CCGG_A
Cuts at: 223 2927 223
Size: 2704 1003
BspHI T'CATG_A
Cuts at: 2883 2883
Size: 3707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 757 971 1273 1654 757
Size: 214 302 381 2810
Fragments arranged by size:
2810 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1999 2053 3537 3702 50
Size: 1949 54 1484 165 55
Fragments arranged by size:
1949 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1619 1619
Size: 3707
BsrFI r'CCGG_y
Cuts at: 1705 1886 2095 1705
Size: 181 209 3317
Fragments arranged by size:
3317 209 181
BssSI C'ACGA_G
Cuts at: 1978 2336 1978
Size: 358 3349
BstAPI GCAn_nnn'nTGC
Cuts at: 1084 1084
Size: 3707
BstBI TT'CG_AA
Cuts at: 2068 2068
Size: 3707
BstYI r'GATC_y
Cuts at: 1 300 732 1199 1222 1557 1803 2815 2901
Size: 299 432 467 23 335 246 1012 86
Cuts at: 2901 2913 3259 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 432 346 335 299 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3707
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1657 1838 2001 1657
Size: 181 163 3363
Fragments arranged by size:
3363 181 163
BtsI GCAGTG_nn'
Cuts at: 950 950
Size: 3707
DraI TTT'AAA
Cuts at: 706 706
Size: 3707
DrdI GACnn_nn'nnGTC
Cuts at: 1413 2271 1413
Size: 858 2849
EaeI y'GGCC_r
Cuts at: 1292 1466 1857 1884 3229 1292
Size: 174 391 27 1345 1770
Fragments arranged by size:
1770 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1292 1292
Size: 3707
EarI CTCTTCn'nnn_
Cuts at: 148 461 1730 1940 148
Size: 313 1269 210 1915
Fragments arranged by size:
1915 1269 313 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 167 1915 2225 2371 3517 167
Size: 1748 310 146 1146 357
Fragments arranged by size:
1748 1146 357 310 146
EcoICRI GAG'CTC
Cuts at: 719 719
Size: 3707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 356 1532 1964 2711 2979 317
Size: 39 1176 432 747 268 1045
Fragments arranged by size:
1176 1045 747 432 268 39
EcoO109I rG'GnC_Cy
Cuts at: 198 256 543 846 198
Size: 58 287 303 3059
Fragments arranged by size:
3059 303 287 58
EcoRI G'AATT_C
Cuts at: 711 711
Size: 3707
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1726 1726
Size: 3707
FspI TGC'GCA
Cuts at: 1488 1488
Size: 3707
HaeII r_GCGC'y
Cuts at: 1141 1389 2411 3390 1141
Size: 248 1022 979 1458
Fragments arranged by size:
1458 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 466 498 1549 1579 1581 1611 466
Size: 32 1051 30 2 30 2562
Fragments arranged by size:
2562 1051 32 30 30 2
HincII GTy'rAC
Cuts at: 746 746
Size: 3707
KasI G'GCGC_C
Cuts at: 1137 1385 1137
Size: 248 3459
KpnI G_GTAC'C
Cuts at: 727 727
Size: 3707
MluI A'CGCG_T
Cuts at: 3140 3140
Size: 3707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 509 2378 2562 509
Size: 1869 184 1654
Fragments arranged by size:
1869 1654 184
MscI TGG'CCA
Cuts at: 1468 1468
Size: 3707
MslI CAynn'nnrTG
Cuts at: 1696 1823 1696
Size: 127 3580
MspA1I CmG'CkG
Cuts at: 818 1132 1492 2505 2750 818
Size: 314 360 1013 245 1775
Fragments arranged by size:
1775 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1888 1888
Size: 3707
NarI GG'CG_CC
Cuts at: 1138 1386 1138
Size: 248 3459
NcoI C'CATG_G
Cuts at: 107 107
Size: 3707
NgoMIV G'CCGG_C
Cuts at: 1886 1886
Size: 3707
NspI r_CATG'y
Cuts at: 760 1260 2167 760
Size: 500 907 2300
Fragments arranged by size:
2300 907 500
PciI A'CATG_T
Cuts at: 2163 2163
Size: 3707
PflMI CCAn_nnn'nTGG
Cuts at: 351 351
Size: 3707
PmeI GTTT'AAAC
Cuts at: 706 706
Size: 3707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2846 2878 2846
Size: 32 3675
PpuMI rG'GwC_Cy
Cuts at: 198 543 198
Size: 345 3362
PshAI GACnn'nnGTC
Cuts at: 181 181
Size: 3707
PspOMI G'GGCC_C
Cuts at: 256 256
Size: 3707
PstI C_TGCA'G
Cuts at: 754 1439 754
Size: 685 3022
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3707
PvuII CAG'CTG
Cuts at: 1132 1492 1132
Size: 360 3347
RsrII CG'GwC_CG
Cuts at: 1902 1902
Size: 3707
SacI G_AGCT'C
Cuts at: 721 721
Size: 3707
SalI G'TCGA_C
Cuts at: 744 744
Size: 3707
SapI GCTCTTCn'nnn_
Cuts at: 148 1730 1940 148
Size: 1582 210 1915
Fragments arranged by size:
1915 1582 210
SbfI CC_TGCA'GG
Cuts at: 754 754
Size: 3707
ScaI AGT'ACT
Cuts at: 3224 3224
Size: 3707
SfcI C'TryA_G
Cuts at: 33 750 1435 2428 2619 33
Size: 717 685 993 191 1121
Fragments arranged by size:
1121 993 717 685 191
SfoI GGC'GCC
Cuts at: 1139 1387 1139
Size: 248 3459
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3707
SmaI CCC'GGG
Cuts at: 261 729 261
Size: 468 3239
SmlI C'TyrA_G
Cuts at: 862 1777 2269 2531 862
Size: 915 492 262 2038
Fragments arranged by size:
2038 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3707
SphI G_CATG'C
Cuts at: 760 1260 760
Size: 500 3207
SrfI GCCC'GGGC
Cuts at: 261 261
Size: 3707
StyI C'CwwG_G
Cuts at: 107 350 841 1195 107
Size: 243 491 354 2619
Fragments arranged by size:
2619 491 354 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 553 1290 1423 1882 2091 553
Size: 737 133 459 209 2169
Fragments arranged by size:
2169 737 459 209 133
TatI w'GTAC_w
Cuts at: 3222 3222
Size: 3707
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3224 3256 3224
Size: 32 3675
Tth111I GACn'n_nGTC
Cuts at: 1504 1504
Size: 3707
XbaI T'CTAG_A
Cuts at: 360 738 360
Size: 378 3329
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1202 1202
Size: 3707
XmaI C'CCGG_G
Cuts at: 259 727 259
Size: 468 3239
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SspI StuI SwaI XhoI XmnI ZraI