Restriction map for
pF1KB6869
(Circular) (Six-base) MAPSORT of: pF1KB6869.seq Check: 9323 from: 1 to: 3650
pF1KB6869 3650 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 688 688
Size: 3650
Acc65I G'GTAC_C
Cuts at: 308 666 308
Size: 358 3292
AclI AA'CG_TT
Cuts at: 3490 3490
Size: 3650
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 1475 1907 2654 2922 382
Size: 1093 432 747 268 1110
Fragments arranged by size:
1110 1093 747 432 268
AflIII A'CryG_T
Cuts at: 1002 2106 3083 1002
Size: 1104 977 1569
Fragments arranged by size:
1569 1104 977
AgeI A'CCGG_T
Cuts at: 311 2038 311
Size: 1727 1923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 389 389
Size: 3650
AlwNI CAG_nnn'CTG
Cuts at: 955 2522 955
Size: 1567 2083
ApaLI G'TGCA_C
Cuts at: 2420 2420
Size: 3650
ApoI r'AATT_y
Cuts at: 191 204 654 3003 3259 191
Size: 13 450 2349 256 582
Fragments arranged by size:
2349 582 450 256 13
AseI AT'TA_AT
Cuts at: 20 20
Size: 3650
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3650
AvaI C'yCGr_G
Cuts at: 670 670
Size: 3650
BamHI G'GATC_C
Cuts at: 675 675
Size: 3650
BanI G'GyrC_C
Cuts at: 308 666 1080 1328 1363 308
Size: 358 414 248 35 2595
Fragments arranged by size:
2595 414 358 248 35
BanII G_rGCy'C
Cuts at: 436 664 1694 436
Size: 228 1030 2392
Fragments arranged by size:
2392 1030 228
BbeI G_GCGC'C
Cuts at: 1084 1332 1084
Size: 248 3402
BciVI GTATCCnnnnn_n'
Cuts at: 1548 2309 3650 1548
Size: 761 1341 1548
Fragments arranged by size:
1548 1341 761
BclI T'GATC_A
Cuts at: 1170 1170
Size: 3650
BglII A'GATC_T
Cuts at: 1165 3202 1165
Size: 2037 1613
BlpI GC'TnA_GC
Cuts at: 762 762
Size: 3650
Bme1580I G_kGCm'C
Cuts at: 1275 1368 2424 1275
Size: 93 1056 2501
Fragments arranged by size:
2501 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 513 862 943 1278 3422 513
Size: 349 81 335 2144 741
Fragments arranged by size:
2144 741 349 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 522 2017 522
Size: 1495 2155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 247 420 227
Size: 20 173 3457
Fragments arranged by size:
3457 173 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 826 1705 2197 2495 826
Size: 879 492 298 1981
Fragments arranged by size:
1981 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3354 3354
Size: 3650
BsaAI yAC'GTr
Cuts at: 1003 1003
Size: 3650
BsaBI GATnn'nnATC
Cuts at: 6 614 1164 3201 6
Size: 608 550 2037 455
Fragments arranged by size:
2037 608 550 455
BsaHI Gr'CG_yC
Cuts at: 1081 1329 2031 3527 1081
Size: 248 702 1496 1204
Fragments arranged by size:
1496 1204 702 248
BsaWI w'CCGG_w
Cuts at: 311 839 1360 2038 2312 2459 2870 311
Size: 528 521 678 274 147 411 1091
Fragments arranged by size:
1091 678 528 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 490 490
Size: 3650
BseYI C'CCAG_C
Cuts at: 1075 1833 2410 2906 1075
Size: 758 577 496 1819
Fragments arranged by size:
1819 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 494
Size: 3650
BsiEI CG_ry'CG
Cuts at: 104 1192 1238 2446 104
Size: 1088 46 1208 1308
Fragments arranged by size:
1308 1208 1088 46
BsiHKAI G_wGCw'C
Cuts at: 436 482 664 1442 1632 2424 436
Size: 46 182 778 190 792 1662
Fragments arranged by size:
1662 792 778 190 182 46
Bsp1286I G_dGCh'C
Cuts at: 436 482 664 1275 1368 1442 1632 1694 2424
Size: 46 182 611 93 74 190 62 730
Cuts at: 2424 436
Size: 1662
Fragments arranged by size:
1662 730 611 190 182 93 74 62
46
BspEI T'CCGG_A
Cuts at: 2870 2870
Size: 3650
BspHI T'CATG_A
Cuts at: 2826 2826
Size: 3650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 700 914 1216 1597 700
Size: 214 302 381 2753
Fragments arranged by size:
2753 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1942 1996 3480 3645 50
Size: 1892 54 1484 165 55
Fragments arranged by size:
1892 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1562 1562
Size: 3650
BsrFI r'CCGG_y
Cuts at: 311 1648 1829 2038 311
Size: 1337 181 209 1923
Fragments arranged by size:
1923 1337 209 181
BssSI C'ACGA_G
Cuts at: 1921 2279 1921
Size: 358 3292
BstAPI GCAn_nnn'nTGC
Cuts at: 1027 1027
Size: 3650
BstBI TT'CG_AA
Cuts at: 2011 2011
Size: 3650
BstYI r'GATC_y
Cuts at: 1 675 1142 1165 1500 1746 2758 2844 2856
Size: 674 467 23 335 246 1012 86 12
Cuts at: 2856 3202 1
Size: 346 449
Fragments arranged by size:
1012 674 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 153 153
Size: 3650
BtgI C'CryG_G
Cuts at: 107 107
Size: 3650
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1600 1781 1944 1600
Size: 181 163 3306
Fragments arranged by size:
3306 181 163
BtsI GCAGTG_nn'
Cuts at: 119 893 119
Size: 774 2876
DraI TTT'AAA
Cuts at: 649 649
Size: 3650
DraIII CAC_nnn'GTG
Cuts at: 131 131
Size: 3650
DrdI GACnn_nn'nnGTC
Cuts at: 1356 2214 1356
Size: 858 2792
EaeI y'GGCC_r
Cuts at: 1235 1409 1800 1827 3172 1235
Size: 174 391 27 1345 1713
Fragments arranged by size:
1713 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1235 1235
Size: 3650
EarI CTCTTCn'nnn_
Cuts at: 1673 1883 1673
Size: 210 3440
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1858 2168 2314 3460 1858
Size: 310 146 1146 2048
Fragments arranged by size:
2048 1146 310 146
EcoICRI GAG'CTC
Cuts at: 434 662 434
Size: 228 3422
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 247 382 420 1475 1907 2654 2922 227
Size: 20 135 38 1055 432 747 268 955
Fragments arranged by size:
1055 955 747 432 268 135 38 20
EcoO109I rG'GnC_Cy
Cuts at: 149 247 789 149
Size: 98 542 3010
Fragments arranged by size:
3010 542 98
EcoRI G'AATT_C
Cuts at: 654 654
Size: 3650
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1669 1669
Size: 3650
FspI TGC'GCA
Cuts at: 1431 1431
Size: 3650
HaeII r_GCGC'y
Cuts at: 1084 1332 2354 3333 1084
Size: 248 1022 979 1401
Fragments arranged by size:
1401 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 423 455 1492 1522 1524 1554 423
Size: 32 1037 30 2 30 2519
Fragments arranged by size:
2519 1037 32 30 30 2
HincII GTy'rAC
Cuts at: 442 689 442
Size: 247 3403
KasI G'GCGC_C
Cuts at: 1080 1328 1080
Size: 248 3402
KpnI G_GTAC'C
Cuts at: 312 670 312
Size: 358 3292
MluI A'CGCG_T
Cuts at: 3083 3083
Size: 3650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2321 2505 2321
Size: 184 3466
MscI TGG'CCA
Cuts at: 1411 1411
Size: 3650
MslI CAynn'nnrTG
Cuts at: 112 371 1639 1766 112
Size: 259 1268 127 1996
Fragments arranged by size:
1996 1268 259 127
MspA1I CmG'CkG
Cuts at: 761 1075 1435 2448 2693 761
Size: 314 360 1013 245 1718
Fragments arranged by size:
1718 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1831 1831
Size: 3650
NarI GG'CG_CC
Cuts at: 1081 1329 1081
Size: 248 3402
NcoI C'CATG_G
Cuts at: 107 107
Size: 3650
NgoMIV G'CCGG_C
Cuts at: 1829 1829
Size: 3650
NspI r_CATG'y
Cuts at: 703 1203 2110 703
Size: 500 907 2243
Fragments arranged by size:
2243 907 500
PciI A'CATG_T
Cuts at: 2106 2106
Size: 3650
PflMI CCAn_nnn'nTGG
Cuts at: 131 131
Size: 3650
PfoI T'CCnGG_A
Cuts at: 243 243
Size: 3650
PmeI GTTT'AAAC
Cuts at: 649 649
Size: 3650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2789 2821 2789
Size: 32 3618
PpuMI rG'GwC_Cy
Cuts at: 149 247 149
Size: 98 3552
PstI C_TGCA'G
Cuts at: 697 1382 697
Size: 685 2965
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3650
PvuII CAG'CTG
Cuts at: 1075 1435 1075
Size: 360 3290
RsrII CG'GwC_CG
Cuts at: 1845 1845
Size: 3650
SacI G_AGCT'C
Cuts at: 436 664 436
Size: 228 3422
SalI G'TCGA_C
Cuts at: 687 687
Size: 3650
SapI GCTCTTCn'nnn_
Cuts at: 1673 1883 1673
Size: 210 3440
SbfI CC_TGCA'GG
Cuts at: 697 697
Size: 3650
ScaI AGT'ACT
Cuts at: 3167 3167
Size: 3650
SfcI C'TryA_G
Cuts at: 33 693 1378 2371 2562 33
Size: 660 685 993 191 1121
Fragments arranged by size:
1121 993 685 660 191
SfoI GGC'GCC
Cuts at: 1082 1330 1082
Size: 248 3402
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3650
SmaI CCC'GGG
Cuts at: 672 672
Size: 3650
SmlI C'TyrA_G
Cuts at: 805 1720 2212 2474 805
Size: 915 492 262 1981
Fragments arranged by size:
1981 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3650
SphI G_CATG'C
Cuts at: 703 1203 703
Size: 500 3150
StyI C'CwwG_G
Cuts at: 107 784 1138 107
Size: 677 354 2619
Fragments arranged by size:
2619 677 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1233 1366 1825 2034 1233
Size: 133 459 209 2849
Fragments arranged by size:
2849 459 209 133
TatI w'GTAC_w
Cuts at: 3165 3165
Size: 3650
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3167 3199 3167
Size: 32 3618
Tth111I GACn'n_nGTC
Cuts at: 1447 1447
Size: 3650
XbaI T'CTAG_A
Cuts at: 681 681
Size: 3650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1145 1145
Size: 3650
XmaI C'CCGG_G
Cuts at: 670 670
Size: 3650
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI