Restriction map for
pF1KB6867
(Circular) (Six-base) MAPSORT of: pF1KB6867.seq Check: 8617 from: 1 to: 3653
pF1KB6867 3653 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 691 691
Size: 3653
Acc65I G'GTAC_C
Cuts at: 669 669
Size: 3653
AclI AA'CG_TT
Cuts at: 3493 3493
Size: 3653
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1478 1910 2657 2925 1478
Size: 432 747 268 2206
Fragments arranged by size:
2206 747 432 268
AfeI AGC'GCT
Cuts at: 117 117
Size: 3653
AflIII A'CryG_T
Cuts at: 1005 2109 3086 1005
Size: 1104 977 1572
Fragments arranged by size:
1572 1104 977
AgeI A'CCGG_T
Cuts at: 2041 2041
Size: 3653
AleI CACnn'nnGTG
Cuts at: 245 245
Size: 3653
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 103 135 103
Size: 32 3621
AlwNI CAG_nnn'CTG
Cuts at: 315 958 2525 315
Size: 643 1567 1443
Fragments arranged by size:
1567 1443 643
ApaI G_GGCC'C
Cuts at: 469 493 578 469
Size: 24 85 3544
Fragments arranged by size:
3544 85 24
ApaLI G'TGCA_C
Cuts at: 2423 2423
Size: 3653
ApoI r'AATT_y
Cuts at: 657 3006 3262 657
Size: 2349 256 1048
Fragments arranged by size:
2349 1048 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3653
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
AvaI C'yCGr_G
Cuts at: 673 673
Size: 3653
BamHI G'GATC_C
Cuts at: 678 678
Size: 3653
BanI G'GyrC_C
Cuts at: 612 669 1083 1331 1366 612
Size: 57 414 248 35 2899
Fragments arranged by size:
2899 414 248 57 35
BanII G_rGCy'C
Cuts at: 469 493 578 667 1697 469
Size: 24 85 89 1030 2425
Fragments arranged by size:
2425 1030 89 85 24
BbeI G_GCGC'C
Cuts at: 1087 1335 1087
Size: 248 3405
BciVI GTATCCnnnnn_n'
Cuts at: 1551 2312 3653 1551
Size: 761 1341 1551
Fragments arranged by size:
1551 1341 761
BclI T'GATC_A
Cuts at: 1173 1173
Size: 3653
BglI GCCn_nnn'nGGC
Cuts at: 621 621
Size: 3653
BglII A'GATC_T
Cuts at: 1168 3205 1168
Size: 2037 1616
BlpI GC'TnA_GC
Cuts at: 765 765
Size: 3653
Bme1580I G_kGCm'C
Cuts at: 469 493 578 617 1278 1371 2427 469
Size: 24 85 39 661 93 1056 1695
Fragments arranged by size:
1695 1056 661 93 85 39 24
BmrI ACTGGGnnnn_n'
Cuts at: 865 946 1281 3425 865
Size: 81 335 2144 1093
Fragments arranged by size:
2144 1093 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2020 2020
Size: 3653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 1708 2200 2498 829
Size: 879 492 298 1984
Fragments arranged by size:
1984 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 582 3357 582
Size: 2775 878
BsaAI yAC'GTr
Cuts at: 1006 1006
Size: 3653
BsaBI GATnn'nnATC
Cuts at: 6 131 1167 3204 6
Size: 125 1036 2037 455
Fragments arranged by size:
2037 1036 455 125
BsaHI Gr'CG_yC
Cuts at: 1084 1332 2034 3530 1084
Size: 248 702 1496 1207
Fragments arranged by size:
1496 1207 702 248
BsaWI w'CCGG_w
Cuts at: 842 1363 2041 2315 2462 2873 842
Size: 521 678 274 147 411 1622
Fragments arranged by size:
1622 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 368 371 419 485 488 368
Size: 3 48 66 3 3533
Fragments arranged by size:
3533 66 48 3 3
BseYI C'CCAG_C
Cuts at: 412 1078 1836 2413 2909 412
Size: 666 758 577 496 1156
Fragments arranged by size:
1156 758 666 577 496
BsiEI CG_ry'CG
Cuts at: 104 1195 1241 2449 104
Size: 1091 46 1208 1308
Fragments arranged by size:
1308 1208 1091 46
BsiHKAI G_wGCw'C
Cuts at: 667 1445 1635 2427 667
Size: 778 190 792 1893
Fragments arranged by size:
1893 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 469 493 578 617 667 1278 1371 1445 1635
Size: 24 85 39 50 611 93 74 190
Cuts at: 1635 1697 2427 469
Size: 62 730 1695
Fragments arranged by size:
1695 730 611 190 93 85 74 62
50 39 24
BspEI T'CCGG_A
Cuts at: 2873 2873
Size: 3653
BspHI T'CATG_A
Cuts at: 2829 2829
Size: 3653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 531 703 917 1219 1600 531
Size: 172 214 302 381 2584
Fragments arranged by size:
2584 381 302 214 172
BsrBI CCG'CTC
Cuts at: 50 1945 1999 3483 3648 50
Size: 1895 54 1484 165 55
Fragments arranged by size:
1895 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1565 1565
Size: 3653
BsrFI r'CCGG_y
Cuts at: 1651 1832 2041 1651
Size: 181 209 3263
Fragments arranged by size:
3263 209 181
BssSI C'ACGA_G
Cuts at: 1924 2282 1924
Size: 358 3295
BstAPI GCAn_nnn'nTGC
Cuts at: 1030 1030
Size: 3653
BstBI TT'CG_AA
Cuts at: 2014 2014
Size: 3653
BstYI r'GATC_y
Cuts at: 1 678 1145 1168 1503 1749 2761 2847 2859
Size: 677 467 23 335 246 1012 86 12
Cuts at: 2859 3205 1
Size: 346 449
Fragments arranged by size:
1012 677 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 122 545 122
Size: 423 3230
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 409 1603 1784 1947 409
Size: 1194 181 163 2115
Fragments arranged by size:
2115 1194 181 163
BtsI GCAGTG_nn'
Cuts at: 240 252 896 240
Size: 12 644 2997
Fragments arranged by size:
2997 644 12
DraI TTT'AAA
Cuts at: 652 652
Size: 3653
DrdI GACnn_nn'nnGTC
Cuts at: 1359 2217 1359
Size: 858 2795
EaeI y'GGCC_r
Cuts at: 1238 1412 1803 1830 3175 1238
Size: 174 391 27 1345 1716
Fragments arranged by size:
1716 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1238 1238
Size: 3653
EarI CTCTTCn'nnn_
Cuts at: 163 293 1676 1886 163
Size: 130 1383 210 1930
Fragments arranged by size:
1930 1383 210 130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1861 2171 2317 3463 1861
Size: 310 146 1146 2051
Fragments arranged by size:
2051 1146 310 146
EcoICRI GAG'CTC
Cuts at: 665 665
Size: 3653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1478 1910 2657 2925 1478
Size: 432 747 268 2206
Fragments arranged by size:
2206 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 386 386
Size: 3653
EcoO109I rG'GnC_Cy
Cuts at: 424 465 466 490 520 575 792 424
Size: 41 1 24 30 55 217 3285
Fragments arranged by size:
3285 217 55 41 30 24 1
EcoRI G'AATT_C
Cuts at: 657 657
Size: 3653
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1672 1672
Size: 3653
FspI TGC'GCA
Cuts at: 1434 1434
Size: 3653
HaeII r_GCGC'y
Cuts at: 119 1087 1335 2357 3336 119
Size: 968 248 1022 979 436
Fragments arranged by size:
1022 979 968 436 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 208 240 390 422 477 509 576 608 1495
Size: 32 150 32 55 32 67 32 887
Cuts at: 1495 1525 1527 1557 208
Size: 30 2 30 2304
Fragments arranged by size:
2304 887 150 67 55 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 692 692
Size: 3653
KasI G'GCGC_C
Cuts at: 1083 1331 1083
Size: 248 3405
KpnI G_GTAC'C
Cuts at: 673 673
Size: 3653
MluI A'CGCG_T
Cuts at: 3086 3086
Size: 3653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2324 2508 2324
Size: 184 3469
MscI TGG'CCA
Cuts at: 1414 1414
Size: 3653
MslI CAynn'nnrTG
Cuts at: 137 245 610 1642 1769 137
Size: 108 365 1032 127 2021
Fragments arranged by size:
2021 1032 365 127 108
MspA1I CmG'CkG
Cuts at: 764 1078 1438 2451 2696 764
Size: 314 360 1013 245 1721
Fragments arranged by size:
1721 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1834 1834
Size: 3653
NarI GG'CG_CC
Cuts at: 1084 1332 1084
Size: 248 3405
NcoI C'CATG_G
Cuts at: 545 545
Size: 3653
NdeI CA'TA_TG
Cuts at: 458 557 458
Size: 99 3554
NgoMIV G'CCGG_C
Cuts at: 1832 1832
Size: 3653
NspI r_CATG'y
Cuts at: 706 1206 2113 706
Size: 500 907 2246
Fragments arranged by size:
2246 907 500
PciI A'CATG_T
Cuts at: 2109 2109
Size: 3653
PfoI T'CCnGG_A
Cuts at: 528 528
Size: 3653
PmeI GTTT'AAAC
Cuts at: 652 652
Size: 3653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2792 2824 2792
Size: 32 3621
PpuMI rG'GwC_Cy
Cuts at: 424 520 424
Size: 96 3557
PspOMI G'GGCC_C
Cuts at: 465 489 574 465
Size: 24 85 3544
Fragments arranged by size:
3544 85 24
PstI C_TGCA'G
Cuts at: 247 390 700 1385 247
Size: 143 310 685 2515
Fragments arranged by size:
2515 685 310 143
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3653
PvuII CAG'CTG
Cuts at: 1078 1438 1078
Size: 360 3293
RsrII CG'GwC_CG
Cuts at: 1848 1848
Size: 3653
SacI G_AGCT'C
Cuts at: 667 667
Size: 3653
SalI G'TCGA_C
Cuts at: 690 690
Size: 3653
SapI GCTCTTCn'nnn_
Cuts at: 293 1676 1886 293
Size: 1383 210 2060
Fragments arranged by size:
2060 1383 210
SbfI CC_TGCA'GG
Cuts at: 390 700 390
Size: 310 3343
ScaI AGT'ACT
Cuts at: 3170 3170
Size: 3653
SfcI C'TryA_G
Cuts at: 33 243 386 696 1381 2374 2565 33
Size: 210 143 310 685 993 191 1121
Fragments arranged by size:
1121 993 685 310 210 191 143
SfoI GGC'GCC
Cuts at: 1085 1333 1085
Size: 248 3405
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
SmaI CCC'GGG
Cuts at: 675 675
Size: 3653
SmlI C'TyrA_G
Cuts at: 808 1723 2215 2477 808
Size: 915 492 262 1984
Fragments arranged by size:
1984 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3653
SphI G_CATG'C
Cuts at: 706 1206 706
Size: 500 3153
StyI C'CwwG_G
Cuts at: 545 787 1141 545
Size: 242 354 3057
Fragments arranged by size:
3057 354 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 226 1236 1369 1828 2037 226
Size: 1010 133 459 209 1842
Fragments arranged by size:
1842 1010 459 209 133
TatI w'GTAC_w
Cuts at: 3168 3168
Size: 3653
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3170 3202 3170
Size: 32 3621
Tth111I GACn'n_nGTC
Cuts at: 1450 1450
Size: 3653
XbaI T'CTAG_A
Cuts at: 684 684
Size: 3653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1148 1148
Size: 3653
XmaI C'CCGG_G
Cuts at: 673 673
Size: 3653
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmgBI BmtI BpmI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI