Restriction map for
pF1KB6862
(Circular) (Six-base) MAPSORT of: pF1KB6862.seq Check: 1515 from: 1 to: 3698
pF1KB6862 3698 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 736 736
Size: 3698
Acc65I G'GTAC_C
Cuts at: 714 714
Size: 3698
AclI AA'CG_TT
Cuts at: 491 3538 491
Size: 3047 651
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 579 1523 1955 2702 2970 202
Size: 377 944 432 747 268 930
Fragments arranged by size:
944 930 747 432 377 268
AflIII A'CryG_T
Cuts at: 1050 2154 3131 1050
Size: 1104 977 1617
Fragments arranged by size:
1617 1104 977
AgeI A'CCGG_T
Cuts at: 2086 2086
Size: 3698
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 482 649 482
Size: 167 3531
AlwNI CAG_nnn'CTG
Cuts at: 1003 2570 1003
Size: 1567 2131
ApaLI G'TGCA_C
Cuts at: 2468 2468
Size: 3698
ApoI r'AATT_y
Cuts at: 702 3051 3307 702
Size: 2349 256 1093
Fragments arranged by size:
2349 1093 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3698
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3698
AvaI C'yCGr_G
Cuts at: 718 718
Size: 3698
BamHI G'GATC_C
Cuts at: 404 723 404
Size: 319 3379
BanI G'GyrC_C
Cuts at: 195 301 714 1128 1376 1411 195
Size: 106 413 414 248 35 2482
Fragments arranged by size:
2482 414 413 248 106 35
BanII G_rGCy'C
Cuts at: 167 311 385 566 712 1742 167
Size: 144 74 181 146 1030 2123
Fragments arranged by size:
2123 1030 181 146 144 74
BbeI G_GCGC'C
Cuts at: 305 1132 1380 305
Size: 827 248 2623
Fragments arranged by size:
2623 827 248
BciVI GTATCCnnnnn_n'
Cuts at: 1596 2357 3698 1596
Size: 761 1341 1596
Fragments arranged by size:
1596 1341 761
BclI T'GATC_A
Cuts at: 1218 1218
Size: 3698
BglII A'GATC_T
Cuts at: 1213 3250 1213
Size: 2037 1661
BlpI GC'TnA_GC
Cuts at: 810 810
Size: 3698
Bme1580I G_kGCm'C
Cuts at: 1323 1416 2472 1323
Size: 93 1056 2549
Fragments arranged by size:
2549 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 654 910 991 1326 3470 654
Size: 256 81 335 2144 882
Fragments arranged by size:
2144 882 335 256 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2065 2065
Size: 3698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 310 407 625 703 163
Size: 147 97 218 78 3158
Fragments arranged by size:
3158 218 147 97 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 874 1753 2245 2543 151
Size: 723 879 492 298 1306
Fragments arranged by size:
1306 879 723 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 286 3402 286
Size: 3116 582
BsaAI yAC'GTr
Cuts at: 1051 1051
Size: 3698
BsaBI GATnn'nnATC
Cuts at: 6 1212 3249 6
Size: 1206 2037 455
Fragments arranged by size:
2037 1206 455
BsaHI Gr'CG_yC
Cuts at: 125 251 302 1129 1377 2079 3575 125
Size: 126 51 827 248 702 1496 248
Fragments arranged by size:
1496 827 702 248 248 126 51
BsaWI w'CCGG_w
Cuts at: 887 1408 2086 2360 2507 2918 887
Size: 521 678 274 147 411 1667
Fragments arranged by size:
1667 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 646 676 646
Size: 30 3668
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 407 628 175
Size: 232 221 3245
Fragments arranged by size:
3245 232 221
BseYI C'CCAG_C
Cuts at: 400 1123 1881 2458 2954 400
Size: 723 758 577 496 1144
Fragments arranged by size:
1144 758 723 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 427
Size: 3698
BsiEI CG_ry'CG
Cuts at: 104 1240 1286 2494 104
Size: 1136 46 1208 1308
Fragments arranged by size:
1308 1208 1136 46
BsiHKAI G_wGCw'C
Cuts at: 167 311 712 1490 1680 2472 167
Size: 144 401 778 190 792 1393
Fragments arranged by size:
1393 792 778 401 190 144
Bsp1286I G_dGCh'C
Cuts at: 167 311 385 566 712 1323 1416 1490 1680
Size: 144 74 181 146 611 93 74 190
Cuts at: 1680 1742 2472 167
Size: 62 730 1393
Fragments arranged by size:
1393 730 611 190 181 146 144 93
74 74 62
BspEI T'CCGG_A
Cuts at: 2918 2918
Size: 3698
BspHI T'CATG_A
Cuts at: 2874 2874
Size: 3698
BspMI ACCTGCnnnn'nnnn_
Cuts at: 631 748 962 1264 1645 631
Size: 117 214 302 381 2684
Fragments arranged by size:
2684 381 302 214 117
BsrBI CCG'CTC
Cuts at: 50 687 1990 2044 3528 3693 50
Size: 637 1303 54 1484 165 55
Fragments arranged by size:
1484 1303 637 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1610 1610
Size: 3698
BsrFI r'CCGG_y
Cuts at: 1696 1877 2086 1696
Size: 181 209 3308
Fragments arranged by size:
3308 209 181
BssHII G'CGCG_C
Cuts at: 114 116 118 233 114
Size: 2 2 115 3579
Fragments arranged by size:
3579 115 2 2
BssSI C'ACGA_G
Cuts at: 1969 2327 1969
Size: 358 3340
BstAPI GCAn_nnn'nTGC
Cuts at: 1075 1075
Size: 3698
BstBI TT'CG_AA
Cuts at: 2059 2059
Size: 3698
BstXI CCAn_nnnn'nTGG
Cuts at: 385 385
Size: 3698
BstYI r'GATC_y
Cuts at: 1 404 723 1190 1213 1548 1794 2806 2892
Size: 403 319 467 23 335 246 1012 86
Cuts at: 2892 2904 3250 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 403 346 335 319 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 649 649
Size: 3698
BtgI C'CryG_G
Cuts at: 107 226 245 107
Size: 119 19 3560
Fragments arranged by size:
3560 119 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1648 1829 1992 1648
Size: 181 163 3354
Fragments arranged by size:
3354 181 163
BtsI GCAGTG_nn'
Cuts at: 557 941 557
Size: 384 3314
DraI TTT'AAA
Cuts at: 697 697
Size: 3698
DrdI GACnn_nn'nnGTC
Cuts at: 274 1404 2262 274
Size: 1130 858 1710
Fragments arranged by size:
1710 1130 858
EaeI y'GGCC_r
Cuts at: 350 368 1283 1457 1848 1875 3220 350
Size: 18 915 174 391 27 1345 828
Fragments arranged by size:
1345 915 828 391 174 27 18
EagI C'GGCC_G
Cuts at: 1283 1283
Size: 3698
EarI CTCTTCn'nnn_
Cuts at: 598 1721 1931 598
Size: 1123 210 2365
Fragments arranged by size:
2365 1123 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1906 2216 2362 3508 1906
Size: 310 146 1146 2096
Fragments arranged by size:
2096 1146 310 146
EcoICRI GAG'CTC
Cuts at: 165 309 710 165
Size: 144 401 3153
Fragments arranged by size:
3153 401 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 202 310 407 579 625 703 1523 1955
Size: 39 108 97 172 46 78 820 432
Cuts at: 1955 2702 2970 163
Size: 747 268 891
Fragments arranged by size:
891 820 747 432 268 172 108 97
78 46 39
EcoNI CCTnn'n_nnAGG
Cuts at: 673 673
Size: 3698
EcoO109I rG'GnC_Cy
Cuts at: 411 525 645 678 837 411
Size: 114 120 33 159 3272
Fragments arranged by size:
3272 159 120 114 33
EcoRI G'AATT_C
Cuts at: 702 702
Size: 3698
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1717 1717
Size: 3698
FspI TGC'GCA
Cuts at: 1479 1479
Size: 3698
HaeII r_GCGC'y
Cuts at: 305 1132 1380 2402 3381 305
Size: 827 248 1022 979 622
Fragments arranged by size:
1022 979 827 622 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 646 678 1540 1570 1572 1602 646
Size: 32 862 30 2 30 2742
Fragments arranged by size:
2742 862 32 30 30 2
HincII GTy'rAC
Cuts at: 737 737
Size: 3698
KasI G'GCGC_C
Cuts at: 301 1128 1376 301
Size: 827 248 2623
Fragments arranged by size:
2623 827 248
KpnI G_GTAC'C
Cuts at: 718 718
Size: 3698
MluI A'CGCG_T
Cuts at: 3131 3131
Size: 3698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2369 2553 2369
Size: 184 3514
MscI TGG'CCA
Cuts at: 1459 1459
Size: 3698
MslI CAynn'nnrTG
Cuts at: 1687 1814 1687
Size: 127 3571
MspA1I CmG'CkG
Cuts at: 201 228 360 400 809 1123 1483 2496 2741
Size: 27 132 40 409 314 360 1013 245
Cuts at: 2741 201
Size: 1158
Fragments arranged by size:
1158 1013 409 360 314 245 132 40
27
NaeI GCC'GGC
Cuts at: 1879 1879
Size: 3698
NarI GG'CG_CC
Cuts at: 302 1129 1377 302
Size: 827 248 2623
Fragments arranged by size:
2623 827 248
NcoI C'CATG_G
Cuts at: 107 245 107
Size: 138 3560
NgoMIV G'CCGG_C
Cuts at: 1877 1877
Size: 3698
NruI TCG'CGA
Cuts at: 478 478
Size: 3698
NspI r_CATG'y
Cuts at: 751 1251 2158 751
Size: 500 907 2291
Fragments arranged by size:
2291 907 500
PciI A'CATG_T
Cuts at: 2154 2154
Size: 3698
PfoI T'CCnGG_A
Cuts at: 131 131
Size: 3698
PmeI GTTT'AAAC
Cuts at: 697 697
Size: 3698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2837 2869 2837
Size: 32 3666
PpuMI rG'GwC_Cy
Cuts at: 525 678 525
Size: 153 3545
PstI C_TGCA'G
Cuts at: 344 365 745 1430 344
Size: 21 380 685 2612
Fragments arranged by size:
2612 685 380 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3698
PvuII CAG'CTG
Cuts at: 360 400 1123 1483 360
Size: 40 723 360 2575
Fragments arranged by size:
2575 723 360 40
RsrII CG'GwC_CG
Cuts at: 1893 1893
Size: 3698
SacI G_AGCT'C
Cuts at: 167 311 712 167
Size: 144 401 3153
Fragments arranged by size:
3153 401 144
SacII CC_GC'GG
Cuts at: 229 229
Size: 3698
SalI G'TCGA_C
Cuts at: 735 735
Size: 3698
SapI GCTCTTCn'nnn_
Cuts at: 598 1721 1931 598
Size: 1123 210 2365
Fragments arranged by size:
2365 1123 210
SbfI CC_TGCA'GG
Cuts at: 344 745 344
Size: 401 3297
ScaI AGT'ACT
Cuts at: 3215 3215
Size: 3698
SexAI A'CCwGG_T
Cuts at: 668 668
Size: 3698
SfcI C'TryA_G
Cuts at: 33 340 361 741 1426 2419 2610 33
Size: 307 21 380 685 993 191 1121
Fragments arranged by size:
1121 993 685 380 307 191 21
SfoI GGC'GCC
Cuts at: 303 1130 1378 303
Size: 827 248 2623
Fragments arranged by size:
2623 827 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3698
SmaI CCC'GGG
Cuts at: 720 720
Size: 3698
SmlI C'TyrA_G
Cuts at: 166 853 1768 2260 2522 166
Size: 687 915 492 262 1342
Fragments arranged by size:
1342 915 687 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3698
SphI G_CATG'C
Cuts at: 751 1251 751
Size: 500 3198
StuI AGG'CCT
Cuts at: 440 440
Size: 3698
StyI C'CwwG_G
Cuts at: 107 245 254 832 1186 107
Size: 138 9 578 354 2619
Fragments arranged by size:
2619 578 354 138 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1281 1414 1873 2082 1281
Size: 133 459 209 2897
Fragments arranged by size:
2897 459 209 133
TatI w'GTAC_w
Cuts at: 3213 3213
Size: 3698
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3215 3247 3215
Size: 32 3666
Tth111I GACn'n_nGTC
Cuts at: 1495 1495
Size: 3698
XbaI T'CTAG_A
Cuts at: 729 729
Size: 3698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 252 614 1193 252
Size: 362 579 2757
Fragments arranged by size:
2757 579 362
XmaI C'CCGG_G
Cuts at: 718 718
Size: 3698
XmnI GAAnn'nnTTC
Cuts at: 592 592
Size: 3698
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PciI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BstEII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI