Restriction map for
pF1KB6861
(Circular) (Six-base) MAPSORT of: pF1KB6861.seq Check: 2550 from: 1 to: 3650
pF1KB6861 3650 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 688 688
Size: 3650
Acc65I G'GTAC_C
Cuts at: 323 666 323
Size: 343 3307
AclI AA'CG_TT
Cuts at: 3490 3490
Size: 3650
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 581 1475 1907 2654 2922 319
Size: 262 894 432 747 268 1047
Fragments arranged by size:
1047 894 747 432 268 262
AflIII A'CryG_T
Cuts at: 1002 2106 3083 1002
Size: 1104 977 1569
Fragments arranged by size:
1569 1104 977
AgeI A'CCGG_T
Cuts at: 2038 2038
Size: 3650
AleI CACnn'nnGTG
Cuts at: 606 606
Size: 3650
AlwNI CAG_nnn'CTG
Cuts at: 297 955 2522 297
Size: 658 1567 1425
Fragments arranged by size:
1567 1425 658
ApaLI G'TGCA_C
Cuts at: 2420 2420
Size: 3650
ApoI r'AATT_y
Cuts at: 316 654 3003 3259 316
Size: 338 2349 256 707
Fragments arranged by size:
2349 707 338 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3650
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3650
AvaI C'yCGr_G
Cuts at: 187 629 670 187
Size: 442 41 3167
Fragments arranged by size:
3167 442 41
BamHI G'GATC_C
Cuts at: 675 675
Size: 3650
BanI G'GyrC_C
Cuts at: 323 370 666 1080 1328 1363 323
Size: 47 296 414 248 35 2610
Fragments arranged by size:
2610 414 296 248 47 35
BanII G_rGCy'C
Cuts at: 246 664 1694 246
Size: 418 1030 2202
Fragments arranged by size:
2202 1030 418
BbeI G_GCGC'C
Cuts at: 1084 1332 1084
Size: 248 3402
BciVI GTATCCnnnnn_n'
Cuts at: 461 1548 2309 3650 461
Size: 1087 761 1341 461
Fragments arranged by size:
1341 1087 761 461
BclI T'GATC_A
Cuts at: 1170 1170
Size: 3650
BglII A'GATC_T
Cuts at: 1165 3202 1165
Size: 2037 1613
BlpI GC'TnA_GC
Cuts at: 762 762
Size: 3650
Bme1580I G_kGCm'C
Cuts at: 373 1275 1368 2424 373
Size: 902 93 1056 1599
Fragments arranged by size:
1599 1056 902 93
BmrI ACTGGGnnnn_n'
Cuts at: 862 943 1278 3422 862
Size: 81 335 2144 1090
Fragments arranged by size:
2144 1090 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2017 2017
Size: 3650
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 826 1705 2197 2495 826
Size: 879 492 298 1981
Fragments arranged by size:
1981 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3354 3354
Size: 3650
BsaAI yAC'GTr
Cuts at: 1003 1003
Size: 3650
BsaBI GATnn'nnATC
Cuts at: 6 1164 3201 6
Size: 1158 2037 455
Fragments arranged by size:
2037 1158 455
BsaHI Gr'CG_yC
Cuts at: 121 1081 1329 2031 3527 121
Size: 960 248 702 1496 244
Fragments arranged by size:
1496 960 702 248 244
BsaWI w'CCGG_w
Cuts at: 839 1360 2038 2312 2459 2870 839
Size: 521 678 274 147 411 1619
Fragments arranged by size:
1619 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 290 503 1075 1833 2410 2906 290
Size: 213 572 758 577 496 1034
Fragments arranged by size:
1034 758 577 572 496 213
BsiEI CG_ry'CG
Cuts at: 104 1192 1238 2446 104
Size: 1088 46 1208 1308
Fragments arranged by size:
1308 1208 1088 46
BsiHKAI G_wGCw'C
Cuts at: 664 1442 1632 2424 664
Size: 778 190 792 1890
Fragments arranged by size:
1890 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 246 373 664 1275 1368 1442 1632 1694 2424
Size: 127 291 611 93 74 190 62 730
Cuts at: 2424 246
Size: 1472
Fragments arranged by size:
1472 730 611 291 190 127 93 74
62
BspEI T'CCGG_A
Cuts at: 2870 2870
Size: 3650
BspHI T'CATG_A
Cuts at: 2826 2826
Size: 3650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 700 914 1216 1597 700
Size: 214 302 381 2753
Fragments arranged by size:
2753 381 302 214
BsrBI CCG'CTC
Cuts at: 50 636 1942 1996 3480 3645 50
Size: 586 1306 54 1484 165 55
Fragments arranged by size:
1484 1306 586 165 55 54
BsrDI GCAATG_nn'
Cuts at: 452 1562 452
Size: 1110 2540
BsrFI r'CCGG_y
Cuts at: 1648 1829 2038 1648
Size: 181 209 3260
Fragments arranged by size:
3260 209 181
BssSI C'ACGA_G
Cuts at: 1921 2279 1921
Size: 358 3292
BstAPI GCAn_nnn'nTGC
Cuts at: 1027 1027
Size: 3650
BstBI TT'CG_AA
Cuts at: 2011 2011
Size: 3650
BstXI CCAn_nnnn'nTGG
Cuts at: 608 608
Size: 3650
BstYI r'GATC_y
Cuts at: 1 675 1142 1165 1500 1746 2758 2844 2856
Size: 674 467 23 335 246 1012 86 12
Cuts at: 2856 3202 1
Size: 346 449
Fragments arranged by size:
1012 674 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 641 107
Size: 534 3116
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1600 1781 1944 1600
Size: 181 163 3306
Fragments arranged by size:
3306 181 163
BtsI GCAGTG_nn'
Cuts at: 893 893
Size: 3650
DraI TTT'AAA
Cuts at: 649 649
Size: 3650
DraIII CAC_nnn'GTG
Cuts at: 547 547
Size: 3650
DrdI GACnn_nn'nnGTC
Cuts at: 1356 2214 1356
Size: 858 2792
EaeI y'GGCC_r
Cuts at: 1235 1409 1800 1827 3172 1235
Size: 174 391 27 1345 1713
Fragments arranged by size:
1713 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1235 1235
Size: 3650
EarI CTCTTCn'nnn_
Cuts at: 1673 1883 1673
Size: 210 3440
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1858 2168 2314 3460 1858
Size: 310 146 1146 2048
Fragments arranged by size:
2048 1146 310 146
EcoICRI GAG'CTC
Cuts at: 662 662
Size: 3650
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 581 1475 1907 2654 2922 319
Size: 262 894 432 747 268 1047
Fragments arranged by size:
1047 894 747 432 268 262
EcoO109I rG'GnC_Cy
Cuts at: 127 402 789 127
Size: 275 387 2988
Fragments arranged by size:
2988 387 275
EcoRI G'AATT_C
Cuts at: 316 654 316
Size: 338 3312
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1669 1669
Size: 3650
FspI TGC'GCA
Cuts at: 1431 1431
Size: 3650
HaeII r_GCGC'y
Cuts at: 178 1084 1332 2354 3333 178
Size: 906 248 1022 979 495
Fragments arranged by size:
1022 979 906 495 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1492 1522 1524 1554 1492
Size: 30 2 30 3588
Fragments arranged by size:
3588 30 30 2
HincII GTy'rAC
Cuts at: 689 689
Size: 3650
HindIII A'AGCT_T
Cuts at: 563 563
Size: 3650
KasI G'GCGC_C
Cuts at: 1080 1328 1080
Size: 248 3402
KpnI G_GTAC'C
Cuts at: 327 670 327
Size: 343 3307
MluI A'CGCG_T
Cuts at: 3083 3083
Size: 3650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2321 2505 2321
Size: 184 3466
MscI TGG'CCA
Cuts at: 1411 1411
Size: 3650
MslI CAynn'nnrTG
Cuts at: 263 606 1639 1766 263
Size: 343 1033 127 2147
Fragments arranged by size:
2147 1033 343 127
MspA1I CmG'CkG
Cuts at: 294 525 761 1075 1435 2448 2693 294
Size: 231 236 314 360 1013 245 1251
Fragments arranged by size:
1251 1013 360 314 245 236 231
NaeI GCC'GGC
Cuts at: 1831 1831
Size: 3650
NarI GG'CG_CC
Cuts at: 1081 1329 1081
Size: 248 3402
NcoI C'CATG_G
Cuts at: 107 641 107
Size: 534 3116
NgoMIV G'CCGG_C
Cuts at: 1829 1829
Size: 3650
NspI r_CATG'y
Cuts at: 703 1203 2110 703
Size: 500 907 2243
Fragments arranged by size:
2243 907 500
PciI A'CATG_T
Cuts at: 2106 2106
Size: 3650
PflMI CCAn_nnn'nTGG
Cuts at: 547 547
Size: 3650
PmeI GTTT'AAAC
Cuts at: 649 649
Size: 3650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2789 2821 2789
Size: 32 3618
PpuMI rG'GwC_Cy
Cuts at: 127 402 127
Size: 275 3375
PsiI TTA'TAA
Cuts at: 167 167
Size: 3650
PspXI vC'TCGA_Gb
Cuts at: 187 187
Size: 3650
PstI C_TGCA'G
Cuts at: 530 697 1382 530
Size: 167 685 2798
Fragments arranged by size:
2798 685 167
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3650
PvuII CAG'CTG
Cuts at: 294 525 1075 1435 294
Size: 231 550 360 2509
Fragments arranged by size:
2509 550 360 231
RsrII CG'GwC_CG
Cuts at: 1845 1845
Size: 3650
SacI G_AGCT'C
Cuts at: 664 664
Size: 3650
SalI G'TCGA_C
Cuts at: 687 687
Size: 3650
SanDI GG'GwC_CC
Cuts at: 127 127
Size: 3650
SapI GCTCTTCn'nnn_
Cuts at: 1673 1883 1673
Size: 210 3440
SbfI CC_TGCA'GG
Cuts at: 697 697
Size: 3650
ScaI AGT'ACT
Cuts at: 3167 3167
Size: 3650
SexAI A'CCwGG_T
Cuts at: 543 543
Size: 3650
SfcI C'TryA_G
Cuts at: 33 526 693 1378 2371 2562 33
Size: 493 167 685 993 191 1121
Fragments arranged by size:
1121 993 685 493 191 167
SfoI GGC'GCC
Cuts at: 1082 1330 1082
Size: 248 3402
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3650
SmaI CCC'GGG
Cuts at: 631 672 631
Size: 41 3609
SmlI C'TyrA_G
Cuts at: 187 805 1720 2212 2474 187
Size: 618 915 492 262 1363
Fragments arranged by size:
1363 915 618 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3650
SphI G_CATG'C
Cuts at: 703 1203 703
Size: 500 3150
StyI C'CwwG_G
Cuts at: 107 641 784 1138 107
Size: 534 143 354 2619
Fragments arranged by size:
2619 534 354 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 304 1233 1366 1825 2034 304
Size: 929 133 459 209 1920
Fragments arranged by size:
1920 929 459 209 133
TatI w'GTAC_w
Cuts at: 3165 3165
Size: 3650
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3167 3199 3167
Size: 32 3618
Tth111I GACn'n_nGTC
Cuts at: 1447 1447
Size: 3650
XbaI T'CTAG_A
Cuts at: 681 681
Size: 3650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1145 114
Size: 1031 2619
XhoI C'TCGA_G
Cuts at: 187 187
Size: 3650
XmaI C'CCGG_G
Cuts at: 629 670 629
Size: 41 3609
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PpiI PpuMI PsiI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PshAI PspOMI PsrI SacII SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XmnI ZraI