Restriction map for
pF1KB6838
(Circular) (Six-base) MAPSORT of: pF1KB6838.seq Check: 250 from: 1 to: 3683
pF1KB6838 3683 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 721 721
Size: 3683
Acc65I G'GTAC_C
Cuts at: 699 699
Size: 3683
AclI AA'CG_TT
Cuts at: 3523 3523
Size: 3683
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1508 1940 2687 2955 1508
Size: 432 747 268 2236
Fragments arranged by size:
2236 747 432 268
AflIII A'CryG_T
Cuts at: 1035 2139 3116 1035
Size: 1104 977 1602
Fragments arranged by size:
1602 1104 977
AgeI A'CCGG_T
Cuts at: 2071 2071
Size: 3683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 254 254
Size: 3683
AlwNI CAG_nnn'CTG
Cuts at: 372 654 988 2555 372
Size: 282 334 1567 1500
Fragments arranged by size:
1567 1500 334 282
ApaLI G'TGCA_C
Cuts at: 2453 2453
Size: 3683
ApoI r'AATT_y
Cuts at: 687 3036 3292 687
Size: 2349 256 1078
Fragments arranged by size:
2349 1078 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3683
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3683
AvaI C'yCGr_G
Cuts at: 703 703
Size: 3683
BamHI G'GATC_C
Cuts at: 708 708
Size: 3683
BanI G'GyrC_C
Cuts at: 699 1113 1361 1396 699
Size: 414 248 35 2986
Fragments arranged by size:
2986 414 248 35
BanII G_rGCy'C
Cuts at: 521 697 1727 521
Size: 176 1030 2477
Fragments arranged by size:
2477 1030 176
BbeI G_GCGC'C
Cuts at: 1117 1365 1117
Size: 248 3435
BbsI GAAGACnn'nnnn_
Cuts at: 169 169
Size: 3683
BciVI GTATCCnnnnn_n'
Cuts at: 1581 2342 3683 1581
Size: 761 1341 1581
Fragments arranged by size:
1581 1341 761
BclI T'GATC_A
Cuts at: 1203 1203
Size: 3683
BglII A'GATC_T
Cuts at: 257 1198 3235 257
Size: 941 2037 705
Fragments arranged by size:
2037 941 705
BlpI GC'TnA_GC
Cuts at: 795 795
Size: 3683
Bme1580I G_kGCm'C
Cuts at: 1308 1401 2457 1308
Size: 93 1056 2534
Fragments arranged by size:
2534 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 218 895 976 1311 3455 218
Size: 677 81 335 2144 446
Fragments arranged by size:
2144 677 446 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2050 2050
Size: 3683
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 1738 2230 2528 859
Size: 879 492 298 2014
Fragments arranged by size:
2014 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 278 3387 278
Size: 3109 574
BsaAI yAC'GTr
Cuts at: 1036 1036
Size: 3683
BsaBI GATnn'nnATC
Cuts at: 6 1197 3234 6
Size: 1191 2037 455
Fragments arranged by size:
2037 1191 455
BsaHI Gr'CG_yC
Cuts at: 1114 1362 2064 3560 1114
Size: 248 702 1496 1237
Fragments arranged by size:
1496 1237 702 248
BsaWI w'CCGG_w
Cuts at: 557 872 1393 2071 2345 2492 2903 557
Size: 315 521 678 274 147 411 1337
Fragments arranged by size:
1337 678 521 411 315 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 541 544 163
Size: 378 3 3302
Fragments arranged by size:
3302 378 3
BseYI C'CCAG_C
Cuts at: 1108 1866 2443 2939 1108
Size: 758 577 496 1852
Fragments arranged by size:
1852 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 667
Size: 3683
BsiEI CG_ry'CG
Cuts at: 104 1225 1271 2479 104
Size: 1121 46 1208 1308
Fragments arranged by size:
1308 1208 1121 46
BsiHKAI G_wGCw'C
Cuts at: 521 697 1475 1665 2457 521
Size: 176 778 190 792 1747
Fragments arranged by size:
1747 792 778 190 176
BsmI GAATG_Cn'
Cuts at: 588 588
Size: 3683
Bsp1286I G_dGCh'C
Cuts at: 521 697 1308 1401 1475 1665 1727 2457 521
Size: 176 611 93 74 190 62 730 1747
Fragments arranged by size:
1747 730 611 190 176 93 74 62
BspEI T'CCGG_A
Cuts at: 2903 2903
Size: 3683
BspHI T'CATG_A
Cuts at: 2859 2859
Size: 3683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 733 947 1249 1630 733
Size: 214 302 381 2786
Fragments arranged by size:
2786 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1975 2029 3513 3678 50
Size: 1925 54 1484 165 55
Fragments arranged by size:
1925 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1595 1595
Size: 3683
BsrFI r'CCGG_y
Cuts at: 1681 1862 2071 1681
Size: 181 209 3293
Fragments arranged by size:
3293 209 181
BssSI C'ACGA_G
Cuts at: 1954 2312 1954
Size: 358 3325
BstAPI GCAn_nnn'nTGC
Cuts at: 1060 1060
Size: 3683
BstBI TT'CG_AA
Cuts at: 2044 2044
Size: 3683
BstEII G'GTnAC_C
Cuts at: 378 378
Size: 3683
BstYI r'GATC_y
Cuts at: 1 257 560 708 1175 1198 1533 1779 2791
Size: 256 303 148 467 23 335 246 1012
Cuts at: 2791 2877 2889 3235 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 303 256 246
148 86 23 12
BtgI C'CryG_G
Cuts at: 107 512 107
Size: 405 3278
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1633 1814 1977 1633
Size: 181 163 3339
Fragments arranged by size:
3339 181 163
BtsI GCAGTG_nn'
Cuts at: 199 926 199
Size: 727 2956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 279 314 279
Size: 35 3648
DraI TTT'AAA
Cuts at: 682 682
Size: 3683
DrdI GACnn_nn'nnGTC
Cuts at: 1389 2247 1389
Size: 858 2825
EaeI y'GGCC_r
Cuts at: 1268 1442 1833 1860 3205 1268
Size: 174 391 27 1345 1746
Fragments arranged by size:
1746 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1268 1268
Size: 3683
EarI CTCTTCn'nnn_
Cuts at: 541 1706 1916 541
Size: 1165 210 2308
Fragments arranged by size:
2308 1165 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1891 2201 2347 3493 1891
Size: 310 146 1146 2081
Fragments arranged by size:
2081 1146 310 146
EcoICRI GAG'CTC
Cuts at: 519 695 519
Size: 176 3507
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1508 1940 2687 2955 1508
Size: 432 747 268 2236
Fragments arranged by size:
2236 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 822 822
Size: 3683
EcoRI G'AATT_C
Cuts at: 687 687
Size: 3683
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 186 288 1702 186
Size: 102 1414 2167
Fragments arranged by size:
2167 1414 102
FspI TGC'GCA
Cuts at: 1464 1464
Size: 3683
HaeII r_GCGC'y
Cuts at: 373 655 1117 1365 2387 3366 373
Size: 282 462 248 1022 979 690
Fragments arranged by size:
1022 979 690 462 282 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1525 1555 1557 1587 1525
Size: 30 2 30 3621
Fragments arranged by size:
3621 30 30 2
HincII GTy'rAC
Cuts at: 722 722
Size: 3683
KasI G'GCGC_C
Cuts at: 1113 1361 1113
Size: 248 3435
KpnI G_GTAC'C
Cuts at: 703 703
Size: 3683
MluI A'CGCG_T
Cuts at: 3116 3116
Size: 3683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 143 2354 2538 143
Size: 2211 184 1288
Fragments arranged by size:
2211 1288 184
MscI TGG'CCA
Cuts at: 1444 1444
Size: 3683
MslI CAynn'nnrTG
Cuts at: 1672 1799 1672
Size: 127 3556
MspA1I CmG'CkG
Cuts at: 234 794 1108 1468 2481 2726 234
Size: 560 314 360 1013 245 1191
Fragments arranged by size:
1191 1013 560 360 314 245
NaeI GCC'GGC
Cuts at: 1864 1864
Size: 3683
NarI GG'CG_CC
Cuts at: 1114 1362 1114
Size: 248 3435
NcoI C'CATG_G
Cuts at: 107 107
Size: 3683
NgoMIV G'CCGG_C
Cuts at: 1862 1862
Size: 3683
NspI r_CATG'y
Cuts at: 736 1236 2143 736
Size: 500 907 2276
Fragments arranged by size:
2276 907 500
PciI A'CATG_T
Cuts at: 2139 2139
Size: 3683
PflMI CCAn_nnn'nTGG
Cuts at: 501 501
Size: 3683
PfoI T'CCnGG_A
Cuts at: 614 614
Size: 3683
PmeI GTTT'AAAC
Cuts at: 682 682
Size: 3683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2822 2854 2822
Size: 32 3651
PstI C_TGCA'G
Cuts at: 608 730 1415 608
Size: 122 685 2876
Fragments arranged by size:
2876 685 122
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3683
PvuII CAG'CTG
Cuts at: 234 1108 1468 234
Size: 874 360 2449
Fragments arranged by size:
2449 874 360
RsrII CG'GwC_CG
Cuts at: 1878 1878
Size: 3683
SacI G_AGCT'C
Cuts at: 521 697 521
Size: 176 3507
SalI G'TCGA_C
Cuts at: 720 720
Size: 3683
SapI GCTCTTCn'nnn_
Cuts at: 1706 1916 1706
Size: 210 3473
SbfI CC_TGCA'GG
Cuts at: 608 730 608
Size: 122 3561
ScaI AGT'ACT
Cuts at: 3200 3200
Size: 3683
SfcI C'TryA_G
Cuts at: 33 604 726 1411 2404 2595 33
Size: 571 122 685 993 191 1121
Fragments arranged by size:
1121 993 685 571 191 122
SfoI GGC'GCC
Cuts at: 1115 1363 1115
Size: 248 3435
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3683
SmaI CCC'GGG
Cuts at: 705 705
Size: 3683
SmlI C'TyrA_G
Cuts at: 838 1753 2245 2507 838
Size: 915 492 262 2014
Fragments arranged by size:
2014 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3683
SphI G_CATG'C
Cuts at: 736 1236 736
Size: 500 3183
StyI C'CwwG_G
Cuts at: 107 626 817 1171 107
Size: 519 191 354 2619
Fragments arranged by size:
2619 519 354 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1266 1399 1858 2067 1266
Size: 133 459 209 2882
Fragments arranged by size:
2882 459 209 133
TatI w'GTAC_w
Cuts at: 3198 3198
Size: 3683
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3200 3232 3200
Size: 32 3651
Tth111I GACn'n_nGTC
Cuts at: 1480 1480
Size: 3683
XbaI T'CTAG_A
Cuts at: 714 714
Size: 3683
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 231 495 1178 231
Size: 264 683 2736
Fragments arranged by size:
2736 683 264
XmaI C'CCGG_G
Cuts at: 703 703
Size: 3683
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII
BstXI BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI