Restriction map for
pF1KB6826
(Circular) (Six-base) MAPSORT of: pF1KB6826.seq Check: 5985 from: 1 to: 3674
pF1KB6826 3674 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AccI GT'mk_AC
Cuts at: 712 712
Size: 3674
Acc65I G'GTAC_C
Cuts at: 690 690
Size: 3674
AclI AA'CG_TT
Cuts at: 668 3514 668
Size: 2846 828
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 589 1499 1931 2678 2946 484
Size: 105 910 432 747 268 1212
Fragments arranged by size:
1212 910 747 432 268 105
AflIII A'CryG_T
Cuts at: 1026 2130 3107 1026
Size: 1104 977 1593
Fragments arranged by size:
1593 1104 977
AgeI A'CCGG_T
Cuts at: 2062 2062
Size: 3674
AlwNI CAG_nnn'CTG
Cuts at: 590 979 2546 590
Size: 389 1567 1718
Fragments arranged by size:
1718 1567 389
ApaLI G'TGCA_C
Cuts at: 2444 2444
Size: 3674
ApoI r'AATT_y
Cuts at: 678 3027 3283 678
Size: 2349 256 1069
Fragments arranged by size:
2349 1069 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3674
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3674
AvaI C'yCGr_G
Cuts at: 694 694
Size: 3674
BamHI G'GATC_C
Cuts at: 699 699
Size: 3674
BanI G'GyrC_C
Cuts at: 690 1104 1352 1387 690
Size: 414 248 35 2977
Fragments arranged by size:
2977 414 248 35
BanII G_rGCy'C
Cuts at: 688 1718 688
Size: 1030 2644
BbeI G_GCGC'C
Cuts at: 1108 1356 1108
Size: 248 3426
BbsI GAAGACnn'nnnn_
Cuts at: 472 472
Size: 3674
BciVI GTATCCnnnnn_n'
Cuts at: 1572 2333 3674 1572
Size: 761 1341 1572
Fragments arranged by size:
1572 1341 761
BclI T'GATC_A
Cuts at: 1194 1194
Size: 3674
BglII A'GATC_T
Cuts at: 1189 3226 1189
Size: 2037 1637
BlpI GC'TnA_GC
Cuts at: 786 786
Size: 3674
Bme1580I G_kGCm'C
Cuts at: 1299 1392 2448 1299
Size: 93 1056 2525
Fragments arranged by size:
2525 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 886 967 1302 3446 886
Size: 81 335 2144 1114
Fragments arranged by size:
2144 1114 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2041 2041
Size: 3674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 508 343
Size: 165 3509
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 850 1729 2221 2519 250
Size: 600 879 492 298 1405
Fragments arranged by size:
1405 879 600 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3378 3378
Size: 3674
BsaAI yAC'GTr
Cuts at: 1027 1027
Size: 3674
BsaBI GATnn'nnATC
Cuts at: 6 1188 3225 6
Size: 1182 2037 455
Fragments arranged by size:
2037 1182 455
BsaHI Gr'CG_yC
Cuts at: 1105 1353 2055 3551 1105
Size: 248 702 1496 1228
Fragments arranged by size:
1496 1228 702 248
BsaWI w'CCGG_w
Cuts at: 863 1384 2062 2336 2483 2894 863
Size: 521 678 274 147 411 1643
Fragments arranged by size:
1643 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 115 310 115
Size: 195 3479
BseYI C'CCAG_C
Cuts at: 1099 1857 2434 2930 1099
Size: 758 577 496 1843
Fragments arranged by size:
1843 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 514
Size: 3674
BsiEI CG_ry'CG
Cuts at: 104 1216 1262 2470 104
Size: 1112 46 1208 1308
Fragments arranged by size:
1308 1208 1112 46
BsiHKAI G_wGCw'C
Cuts at: 688 1466 1656 2448 688
Size: 778 190 792 1914
Fragments arranged by size:
1914 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 688 1299 1392 1466 1656 1718 2448 688
Size: 611 93 74 190 62 730 1914
Fragments arranged by size:
1914 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2894 2894
Size: 3674
BspHI T'CATG_A
Cuts at: 518 2850 518
Size: 2332 1342
BspMI ACCTGCnnnn'nnnn_
Cuts at: 724 938 1240 1621 724
Size: 214 302 381 2777
Fragments arranged by size:
2777 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1966 2020 3504 3669 50
Size: 1916 54 1484 165 55
Fragments arranged by size:
1916 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1586 1586
Size: 3674
BsrFI r'CCGG_y
Cuts at: 1672 1853 2062 1672
Size: 181 209 3284
Fragments arranged by size:
3284 209 181
BssSI C'ACGA_G
Cuts at: 1945 2303 1945
Size: 358 3316
BstAPI GCAn_nnn'nTGC
Cuts at: 1051 1051
Size: 3674
BstBI TT'CG_AA
Cuts at: 2035 2035
Size: 3674
BstEII G'GTnAC_C
Cuts at: 655 655
Size: 3674
BstYI r'GATC_y
Cuts at: 1 699 1166 1189 1524 1770 2782 2868 2880
Size: 698 467 23 335 246 1012 86 12
Cuts at: 2880 3226 1
Size: 346 449
Fragments arranged by size:
1012 698 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 293 622 293
Size: 329 3345
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1624 1805 1968 1624
Size: 181 163 3330
Fragments arranged by size:
3330 181 163
BtsI GCAGTG_nn'
Cuts at: 917 917
Size: 3674
DraI TTT'AAA
Cuts at: 673 673
Size: 3674
DrdI GACnn_nn'nnGTC
Cuts at: 1380 2238 1380
Size: 858 2816
EaeI y'GGCC_r
Cuts at: 1259 1433 1824 1851 3196 1259
Size: 174 391 27 1345 1737
Fragments arranged by size:
1737 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1259 1259
Size: 3674
EarI CTCTTCn'nnn_
Cuts at: 1697 1907 1697
Size: 210 3464
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1882 2192 2338 3484 1882
Size: 310 146 1146 2072
Fragments arranged by size:
2072 1146 310 146
EcoICRI GAG'CTC
Cuts at: 686 686
Size: 3674
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 484 508 589 1499 1931 2678 2946 343
Size: 141 24 81 910 432 747 268 1071
Fragments arranged by size:
1071 910 747 432 268 141 81 24
EcoO109I rG'GnC_Cy
Cuts at: 813 813
Size: 3674
EcoRI G'AATT_C
Cuts at: 678 678
Size: 3674
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1693 1693
Size: 3674
FspI TGC'GCA
Cuts at: 1455 1455
Size: 3674
HaeII r_GCGC'y
Cuts at: 1108 1356 2378 3357 1108
Size: 248 1022 979 1425
Fragments arranged by size:
1425 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 444 476 602 634 1516 1546 1548 1578 444
Size: 32 126 32 882 30 2 30 2540
Fragments arranged by size:
2540 882 126 32 32 30 30 2
HincII GTy'rAC
Cuts at: 713 713
Size: 3674
KasI G'GCGC_C
Cuts at: 1104 1352 1104
Size: 248 3426
KpnI G_GTAC'C
Cuts at: 694 694
Size: 3674
MfeI C'AATT_G
Cuts at: 238 238
Size: 3674
MluI A'CGCG_T
Cuts at: 3107 3107
Size: 3674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2345 2529 2345
Size: 184 3490
MscI TGG'CCA
Cuts at: 1435 1435
Size: 3674
MslI CAynn'nnrTG
Cuts at: 1663 1790 1663
Size: 127 3547
MspA1I CmG'CkG
Cuts at: 309 785 1099 1459 2472 2717 309
Size: 476 314 360 1013 245 1266
Fragments arranged by size:
1266 1013 476 360 314 245
NaeI GCC'GGC
Cuts at: 1855 1855
Size: 3674
NarI GG'CG_CC
Cuts at: 1105 1353 1105
Size: 248 3426
NgoMIV G'CCGG_C
Cuts at: 1853 1853
Size: 3674
NspI r_CATG'y
Cuts at: 727 1227 2134 727
Size: 500 907 2267
Fragments arranged by size:
2267 907 500
PciI A'CATG_T
Cuts at: 2130 2130
Size: 3674
PfoI T'CCnGG_A
Cuts at: 473 473
Size: 3674
PmeI GTTT'AAAC
Cuts at: 673 673
Size: 3674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2813 2845 2813
Size: 32 3642
PshAI GACnn'nnGTC
Cuts at: 604 604
Size: 3674
PstI C_TGCA'G
Cuts at: 314 721 1406 314
Size: 407 685 2582
Fragments arranged by size:
2582 685 407
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3674
PvuII CAG'CTG
Cuts at: 309 1099 1459 309
Size: 790 360 2524
Fragments arranged by size:
2524 790 360
RsrII CG'GwC_CG
Cuts at: 1869 1869
Size: 3674
SacI G_AGCT'C
Cuts at: 688 688
Size: 3674
SalI G'TCGA_C
Cuts at: 711 711
Size: 3674
SapI GCTCTTCn'nnn_
Cuts at: 1697 1907 1697
Size: 210 3464
SbfI CC_TGCA'GG
Cuts at: 721 721
Size: 3674
ScaI AGT'ACT
Cuts at: 3191 3191
Size: 3674
SfcI C'TryA_G
Cuts at: 33 158 310 717 1402 2395 2586 33
Size: 125 152 407 685 993 191 1121
Fragments arranged by size:
1121 993 685 407 191 152 125
SfoI GGC'GCC
Cuts at: 1106 1354 1106
Size: 248 3426
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3674
SmaI CCC'GGG
Cuts at: 696 696
Size: 3674
SmlI C'TyrA_G
Cuts at: 265 829 1744 2236 2498 265
Size: 564 915 492 262 1441
Fragments arranged by size:
1441 915 564 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3674
SphI G_CATG'C
Cuts at: 727 1227 727
Size: 500 3174
StyI C'CwwG_G
Cuts at: 575 808 1162 575
Size: 233 354 3087
Fragments arranged by size:
3087 354 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1257 1390 1849 2058 1257
Size: 133 459 209 2873
Fragments arranged by size:
2873 459 209 133
TatI w'GTAC_w
Cuts at: 581 3189 581
Size: 2608 1066
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 3642
Tth111I GACn'n_nGTC
Cuts at: 1471 1471
Size: 3674
XbaI T'CTAG_A
Cuts at: 705 705
Size: 3674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 609 1169 609
Size: 560 3114
XmaI C'CCGG_G
Cuts at: 694 694
Size: 3674
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI
PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstXI BstZ17I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI