Restriction map for
pF1KB6811
(Circular) (Six-base) MAPSORT of: pF1KB6811.seq Check: 1875 from: 1 to: 3593
pF1KB6811 3593 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 631 631
Size: 3593
Acc65I G'GTAC_C
Cuts at: 609 609
Size: 3593
AclI AA'CG_TT
Cuts at: 3433 3433
Size: 3593
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 1418 1850 2597 2865 562
Size: 856 432 747 268 1290
Fragments arranged by size:
1290 856 747 432 268
AflIII A'CryG_T
Cuts at: 585 945 2049 3026 585
Size: 360 1104 977 1152
Fragments arranged by size:
1152 1104 977 360
AgeI A'CCGG_T
Cuts at: 1981 1981
Size: 3593
AlwNI CAG_nnn'CTG
Cuts at: 399 411 530 898 2465 399
Size: 12 119 368 1567 1527
Fragments arranged by size:
1567 1527 368 119 12
ApaLI G'TGCA_C
Cuts at: 2363 2363
Size: 3593
ApoI r'AATT_y
Cuts at: 597 2946 3202 597
Size: 2349 256 988
Fragments arranged by size:
2349 988 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3593
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3593
AvaI C'yCGr_G
Cuts at: 449 613 449
Size: 164 3429
BamHI G'GATC_C
Cuts at: 618 618
Size: 3593
BanI G'GyrC_C
Cuts at: 436 609 1023 1271 1306 436
Size: 173 414 248 35 2723
Fragments arranged by size:
2723 414 248 173 35
BanII G_rGCy'C
Cuts at: 125 607 1637 125
Size: 482 1030 2081
Fragments arranged by size:
2081 1030 482
BbeI G_GCGC'C
Cuts at: 1027 1275 1027
Size: 248 3345
BciVI GTATCCnnnnn_n'
Cuts at: 1491 2252 3593 1491
Size: 761 1341 1491
Fragments arranged by size:
1491 1341 761
BclI T'GATC_A
Cuts at: 1113 1113
Size: 3593
BglII A'GATC_T
Cuts at: 1108 3145 1108
Size: 2037 1556
BlpI GC'TnA_GC
Cuts at: 197 705 197
Size: 508 3085
Bme1580I G_kGCm'C
Cuts at: 439 1218 1311 2367 439
Size: 779 93 1056 1665
Fragments arranged by size:
1665 1056 779 93
BmrI ACTGGGnnnn_n'
Cuts at: 805 886 1221 3365 805
Size: 81 335 2144 1033
Fragments arranged by size:
2144 1033 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1960 1960
Size: 3593
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 1648 2140 2438 769
Size: 879 492 298 1924
Fragments arranged by size:
1924 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 421 446 3297 421
Size: 25 2851 717
Fragments arranged by size:
2851 717 25
BsaAI yAC'GTr
Cuts at: 946 946
Size: 3593
BsaBI GATnn'nnATC
Cuts at: 6 1107 3144 6
Size: 1101 2037 455
Fragments arranged by size:
2037 1101 455
BsaHI Gr'CG_yC
Cuts at: 491 1024 1272 1974 3470 491
Size: 533 248 702 1496 614
Fragments arranged by size:
1496 702 614 533 248
BsaWI w'CCGG_w
Cuts at: 782 1303 1981 2255 2402 2813 782
Size: 521 678 274 147 411 1562
Fragments arranged by size:
1562 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 310 313 316 193
Size: 117 3 3 3470
Fragments arranged by size:
3470 117 3 3
BseYI C'CCAG_C
Cuts at: 1018 1776 2353 2849 1018
Size: 758 577 496 1762
Fragments arranged by size:
1762 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 425 1135 1181 2389 104
Size: 321 710 46 1208 1308
Fragments arranged by size:
1308 1208 710 321 46
BsiHKAI G_wGCw'C
Cuts at: 125 607 1385 1575 2367 125
Size: 482 778 190 792 1351
Fragments arranged by size:
1351 792 778 482 190
Bsp1286I G_dGCh'C
Cuts at: 125 439 607 1218 1311 1385 1575 1637 2367
Size: 314 168 611 93 74 190 62 730
Cuts at: 2367 125
Size: 1351
Fragments arranged by size:
1351 730 611 314 190 168 93 74
62
BspEI T'CCGG_A
Cuts at: 2813 2813
Size: 3593
BspHI T'CATG_A
Cuts at: 2769 2769
Size: 3593
BspMI ACCTGCnnnn'nnnn_
Cuts at: 464 643 857 1159 1540 464
Size: 179 214 302 381 2517
Fragments arranged by size:
2517 381 302 214 179
BsrBI CCG'CTC
Cuts at: 50 196 1885 1939 3423 3588 50
Size: 146 1689 54 1484 165 55
Fragments arranged by size:
1689 1484 165 146 55 54
BsrDI GCAATG_nn'
Cuts at: 1505 1505
Size: 3593
BsrFI r'CCGG_y
Cuts at: 420 1591 1772 1981 420
Size: 1171 181 209 2032
Fragments arranged by size:
2032 1171 209 181
BssSI C'ACGA_G
Cuts at: 124 1864 2222 124
Size: 1740 358 1495
Fragments arranged by size:
1740 1495 358
BstAPI GCAn_nnn'nTGC
Cuts at: 153 970 153
Size: 817 2776
BstBI TT'CG_AA
Cuts at: 1954 1954
Size: 3593
BstEII G'GTnAC_C
Cuts at: 162 162
Size: 3593
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3593
BstYI r'GATC_y
Cuts at: 1 618 1085 1108 1443 1689 2701 2787 2799
Size: 617 467 23 335 246 1012 86 12
Cuts at: 2799 3145 1
Size: 346 449
Fragments arranged by size:
1012 617 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 170 504 170
Size: 334 3259
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 297 1543 1724 1887 297
Size: 1246 181 163 2003
Fragments arranged by size:
2003 1246 181 163
BtsI GCAGTG_nn'
Cuts at: 836 836
Size: 3593
DraI TTT'AAA
Cuts at: 592 592
Size: 3593
DrdI GACnn_nn'nnGTC
Cuts at: 268 1299 2157 268
Size: 1031 858 1704
Fragments arranged by size:
1704 1031 858
EaeI y'GGCC_r
Cuts at: 191 236 284 422 521 1178 1352 1743 1770
Size: 45 48 138 99 657 174 391 27
Cuts at: 1770 3115 191
Size: 1345 669
Fragments arranged by size:
1345 669 657 391 174 138 99 48
45 27
EagI C'GGCC_G
Cuts at: 422 1178 422
Size: 756 2837
EarI CTCTTCn'nnn_
Cuts at: 113 493 581 1616 1826 113
Size: 380 88 1035 210 1880
Fragments arranged by size:
1880 1035 380 210 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1801 2111 2257 3403 1801
Size: 310 146 1146 1991
Fragments arranged by size:
1991 1146 310 146
EcoICRI GAG'CTC
Cuts at: 123 605 123
Size: 482 3111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 1418 1850 2597 2865 562
Size: 856 432 747 268 1290
Fragments arranged by size:
1290 856 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 561 732 561
Size: 171 3422
EcoRI G'AATT_C
Cuts at: 597 597
Size: 3593
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1612 1612
Size: 3593
FspI TGC'GCA
Cuts at: 1374 1374
Size: 3593
HaeII r_GCGC'y
Cuts at: 204 399 1027 1275 2297 3276 204
Size: 195 628 248 1022 979 521
Fragments arranged by size:
1022 979 628 521 248 195
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1435 1465 1467 1497 1435
Size: 30 2 30 3531
Fragments arranged by size:
3531 30 30 2
HincII GTy'rAC
Cuts at: 632 632
Size: 3593
KasI G'GCGC_C
Cuts at: 1023 1271 1023
Size: 248 3345
KpnI G_GTAC'C
Cuts at: 613 613
Size: 3593
MluI A'CGCG_T
Cuts at: 585 3026 585
Size: 2441 1152
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2264 2448 2264
Size: 184 3409
MscI TGG'CCA
Cuts at: 286 523 1354 286
Size: 237 831 2525
Fragments arranged by size:
2525 831 237
MslI CAynn'nnrTG
Cuts at: 1582 1709 1582
Size: 127 3466
MspA1I CmG'CkG
Cuts at: 172 704 1018 1378 2391 2636 172
Size: 532 314 360 1013 245 1129
Fragments arranged by size:
1129 1013 532 360 314 245
NaeI GCC'GGC
Cuts at: 422 1774 422
Size: 1352 2241
NarI GG'CG_CC
Cuts at: 1024 1272 1024
Size: 248 3345
NgoMIV G'CCGG_C
Cuts at: 420 1772 420
Size: 1352 2241
NspI r_CATG'y
Cuts at: 393 646 1146 2053 393
Size: 253 500 907 1933
Fragments arranged by size:
1933 907 500 253
PasI CC'CwG_GG
Cuts at: 432 432
Size: 3593
PciI A'CATG_T
Cuts at: 2049 2049
Size: 3593
PflMI CCAn_nnn'nTGG
Cuts at: 510 510
Size: 3593
PmeI GTTT'AAAC
Cuts at: 592 592
Size: 3593
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2732 2764 2732
Size: 32 3561
PpuMI rG'GwC_Cy
Cuts at: 561 561
Size: 3593
PstI C_TGCA'G
Cuts at: 640 1325 640
Size: 685 2908
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3593
PvuII CAG'CTG
Cuts at: 1018 1378 1018
Size: 360 3233
RsrII CG'GwC_CG
Cuts at: 1788 1788
Size: 3593
SacI G_AGCT'C
Cuts at: 125 607 125
Size: 482 3111
SacII CC_GC'GG
Cuts at: 173 173
Size: 3593
SalI G'TCGA_C
Cuts at: 630 630
Size: 3593
SanDI GG'GwC_CC
Cuts at: 561 561
Size: 3593
SapI GCTCTTCn'nnn_
Cuts at: 113 493 1616 1826 113
Size: 380 1123 210 1880
Fragments arranged by size:
1880 1123 380 210
SbfI CC_TGCA'GG
Cuts at: 640 640
Size: 3593
ScaI AGT'ACT
Cuts at: 3110 3110
Size: 3593
SfcI C'TryA_G
Cuts at: 33 636 1321 2314 2505 33
Size: 603 685 993 191 1121
Fragments arranged by size:
1121 993 685 603 191
SfoI GGC'GCC
Cuts at: 1025 1273 1025
Size: 248 3345
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3593
SmaI CCC'GGG
Cuts at: 615 615
Size: 3593
SmlI C'TyrA_G
Cuts at: 748 1663 2155 2417 748
Size: 915 492 262 1924
Fragments arranged by size:
1924 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3593
SphI G_CATG'C
Cuts at: 393 646 1146 393
Size: 253 500 2840
Fragments arranged by size:
2840 500 253
StyI C'CwwG_G
Cuts at: 727 1081 727
Size: 354 3239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1176 1309 1768 1977 1176
Size: 133 459 209 2792
Fragments arranged by size:
2792 459 209 133
TatI w'GTAC_w
Cuts at: 3108 3108
Size: 3593
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3110 3142 3110
Size: 32 3561
Tth111I GACn'n_nGTC
Cuts at: 1390 1390
Size: 3593
XbaI T'CTAG_A
Cuts at: 624 624
Size: 3593
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1088 1088
Size: 3593
XmaI C'CCGG_G
Cuts at: 613 613
Size: 3593
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI