Restriction map for
pF1KB6807
(Circular) (Six-base) MAPSORT of: pF1KB6807.seq Check: 8683 from: 1 to: 3590
pF1KB6807 3590 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 628 628
Size: 3590
Acc65I G'GTAC_C
Cuts at: 606 606
Size: 3590
AclI AA'CG_TT
Cuts at: 3430 3430
Size: 3590
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 563 1415 1847 2594 2862 515
Size: 48 852 432 747 268 1243
Fragments arranged by size:
1243 852 747 432 268 48
AflII C'TTAA_G
Cuts at: 202 202
Size: 3590
AflIII A'CryG_T
Cuts at: 942 2046 3023 942
Size: 1104 977 1509
Fragments arranged by size:
1509 1104 977
AgeI A'CCGG_T
Cuts at: 1978 1978
Size: 3590
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 205 237 205
Size: 32 3558
AlwNI CAG_nnn'CTG
Cuts at: 895 2462 895
Size: 1567 2023
ApaLI G'TGCA_C
Cuts at: 305 2360 305
Size: 2055 1535
ApoI r'AATT_y
Cuts at: 594 2943 3199 594
Size: 2349 256 985
Fragments arranged by size:
2349 985 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3590
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3590
AvaI C'yCGr_G
Cuts at: 610 610
Size: 3590
BamHI G'GATC_C
Cuts at: 615 615
Size: 3590
BanI G'GyrC_C
Cuts at: 606 1020 1268 1303 606
Size: 414 248 35 2893
Fragments arranged by size:
2893 414 248 35
BanII G_rGCy'C
Cuts at: 455 604 1634 455
Size: 149 1030 2411
Fragments arranged by size:
2411 1030 149
BbeI G_GCGC'C
Cuts at: 1024 1272 1024
Size: 248 3342
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 376 410 376
Size: 34 3556
BciVI GTATCCnnnnn_n'
Cuts at: 1488 2249 3590 1488
Size: 761 1341 1488
Fragments arranged by size:
1488 1341 761
BclI T'GATC_A
Cuts at: 1110 1110
Size: 3590
BglII A'GATC_T
Cuts at: 1105 3142 1105
Size: 2037 1553
BlpI GC'TnA_GC
Cuts at: 702 702
Size: 3590
Bme1580I G_kGCm'C
Cuts at: 309 438 1215 1308 2364 309
Size: 129 777 93 1056 1535
Fragments arranged by size:
1535 1056 777 129 93
BmrI ACTGGGnnnn_n'
Cuts at: 802 883 1218 3362 802
Size: 81 335 2144 1030
Fragments arranged by size:
2144 1030 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1957 1957
Size: 3590
Bpu10I CC'TnA_GC
Cuts at: 454 542 454
Size: 88 3502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 1645 2137 2435 766
Size: 879 492 298 1921
Fragments arranged by size:
1921 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3294 3294
Size: 3590
BsaAI yAC'GTr
Cuts at: 310 943 310
Size: 633 2957
BsaBI GATnn'nnATC
Cuts at: 6 195 1104 3141 6
Size: 189 909 2037 455
Fragments arranged by size:
2037 909 455 189
BsaHI Gr'CG_yC
Cuts at: 1021 1269 1971 3467 1021
Size: 248 702 1496 1144
Fragments arranged by size:
1496 1144 702 248
BsaWI w'CCGG_w
Cuts at: 779 1300 1978 2252 2399 2810 779
Size: 521 678 274 147 411 1559
Fragments arranged by size:
1559 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 525 1015 1773 2350 2846 525
Size: 490 758 577 496 1269
Fragments arranged by size:
1269 758 577 496 490
BsiEI CG_ry'CG
Cuts at: 104 390 1132 1178 2386 104
Size: 286 742 46 1208 1308
Fragments arranged by size:
1308 1208 742 286 46
BsiHKAI G_wGCw'C
Cuts at: 309 432 604 1382 1572 2364 309
Size: 123 172 778 190 792 1535
Fragments arranged by size:
1535 792 778 190 172 123
Bsp1286I G_dGCh'C
Cuts at: 309 432 438 455 604 1215 1308 1382 1572
Size: 123 6 17 149 611 93 74 190
Cuts at: 1572 1634 2364 309
Size: 62 730 1535
Fragments arranged by size:
1535 730 611 190 149 123 93 74
62 17 6
BspEI T'CCGG_A
Cuts at: 2810 2810
Size: 3590
BspHI T'CATG_A
Cuts at: 2766 2766
Size: 3590
BspMI ACCTGCnnnn'nnnn_
Cuts at: 640 854 1156 1537 640
Size: 214 302 381 2693
Fragments arranged by size:
2693 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1882 1936 3420 3585 50
Size: 1832 54 1484 165 55
Fragments arranged by size:
1832 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 401 1502 401
Size: 1101 2489
BsrFI r'CCGG_y
Cuts at: 464 1588 1769 1978 464
Size: 1124 181 209 2076
Fragments arranged by size:
2076 1124 209 181
BssSI C'ACGA_G
Cuts at: 431 1861 2219 431
Size: 1430 358 1802
Fragments arranged by size:
1802 1430 358
BstAPI GCAn_nnn'nTGC
Cuts at: 967 967
Size: 3590
BstBI TT'CG_AA
Cuts at: 1951 1951
Size: 3590
BstYI r'GATC_y
Cuts at: 1 359 615 1082 1105 1440 1686 2698 2784
Size: 358 256 467 23 335 246 1012 86
Cuts at: 2784 2796 3142 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 358 346 335 256 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 226 226
Size: 3590
BtgI C'CryG_G
Cuts at: 107 107
Size: 3590
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1540 1721 1884 1540
Size: 181 163 3246
Fragments arranged by size:
3246 181 163
BtsI GCAGTG_nn'
Cuts at: 833 833
Size: 3590
DraI TTT'AAA
Cuts at: 589 589
Size: 3590
DrdI GACnn_nn'nnGTC
Cuts at: 1296 2154 1296
Size: 858 2732
EaeI y'GGCC_r
Cuts at: 110 466 1175 1349 1740 1767 3112 110
Size: 356 709 174 391 27 1345 588
Fragments arranged by size:
1345 709 588 391 356 174 27
EagI C'GGCC_G
Cuts at: 1175 1175
Size: 3590
EarI CTCTTCn'nnn_
Cuts at: 1613 1823 1613
Size: 210 3380
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1798 2108 2254 3400 1798
Size: 310 146 1146 1988
Fragments arranged by size:
1988 1146 310 146
EcoICRI GAG'CTC
Cuts at: 602 602
Size: 3590
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 563 1415 1847 2594 2862 515
Size: 48 852 432 747 268 1243
Fragments arranged by size:
1243 852 747 432 268 48
EcoO109I rG'GnC_Cy
Cuts at: 264 327 564 729 264
Size: 63 237 165 3125
Fragments arranged by size:
3125 237 165 63
EcoRI G'AATT_C
Cuts at: 594 594
Size: 3590
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1609 1609
Size: 3590
FspI TGC'GCA
Cuts at: 1371 1371
Size: 3590
HaeII r_GCGC'y
Cuts at: 1024 1272 2294 3273 1024
Size: 248 1022 979 1341
Fragments arranged by size:
1341 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1432 1462 1464 1494 1432
Size: 30 2 30 3528
Fragments arranged by size:
3528 30 30 2
HincII GTy'rAC
Cuts at: 629 629
Size: 3590
HindIII A'AGCT_T
Cuts at: 415 415
Size: 3590
KasI G'GCGC_C
Cuts at: 1020 1268 1020
Size: 248 3342
KpnI G_GTAC'C
Cuts at: 610 610
Size: 3590
MluI A'CGCG_T
Cuts at: 3023 3023
Size: 3590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2261 2445 2261
Size: 184 3406
MscI TGG'CCA
Cuts at: 112 1351 112
Size: 1239 2351
MslI CAynn'nnrTG
Cuts at: 161 1579 1706 161
Size: 1418 127 2045
Fragments arranged by size:
2045 1418 127
MspA1I CmG'CkG
Cuts at: 701 1015 1375 2388 2633 701
Size: 314 360 1013 245 1658
Fragments arranged by size:
1658 1013 360 314 245
NaeI GCC'GGC
Cuts at: 466 1771 466
Size: 1305 2285
NarI GG'CG_CC
Cuts at: 1021 1269 1021
Size: 248 3342
NcoI C'CATG_G
Cuts at: 107 107
Size: 3590
NgoMIV G'CCGG_C
Cuts at: 464 1769 464
Size: 1305 2285
NspI r_CATG'y
Cuts at: 237 643 1143 2050 237
Size: 406 500 907 1777
Fragments arranged by size:
1777 907 500 406
PciI A'CATG_T
Cuts at: 2046 2046
Size: 3590
PmeI GTTT'AAAC
Cuts at: 589 589
Size: 3590
PmlI CAC'GTG
Cuts at: 310 310
Size: 3590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2729 2761 2729
Size: 32 3558
PstI C_TGCA'G
Cuts at: 522 637 1322 522
Size: 115 685 2790
Fragments arranged by size:
2790 685 115
PvuI CG_AT'CG
Cuts at: 104 390 104
Size: 286 3304
PvuII CAG'CTG
Cuts at: 1015 1375 1015
Size: 360 3230
RsrII CG'GwC_CG
Cuts at: 1785 1785
Size: 3590
SacI G_AGCT'C
Cuts at: 604 604
Size: 3590
SalI G'TCGA_C
Cuts at: 627 627
Size: 3590
SapI GCTCTTCn'nnn_
Cuts at: 1613 1823 1613
Size: 210 3380
SbfI CC_TGCA'GG
Cuts at: 637 637
Size: 3590
ScaI AGT'ACT
Cuts at: 3107 3107
Size: 3590
SfcI C'TryA_G
Cuts at: 33 518 633 1318 2311 2502 33
Size: 485 115 685 993 191 1121
Fragments arranged by size:
1121 993 685 485 191 115
SfoI GGC'GCC
Cuts at: 1022 1270 1022
Size: 248 3342
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3590
SmaI CCC'GGG
Cuts at: 612 612
Size: 3590
SmlI C'TyrA_G
Cuts at: 202 745 1660 2152 2414 202
Size: 543 915 492 262 1378
Fragments arranged by size:
1378 915 543 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3590
SphI G_CATG'C
Cuts at: 643 1143 643
Size: 500 3090
StyI C'CwwG_G
Cuts at: 107 293 341 446 476 482 488 494 506
Size: 186 48 105 30 6 6 6 12
Cuts at: 506 724 1078 107
Size: 218 354 2619
Fragments arranged by size:
2619 354 218 186 105 48 30 12
6 6 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1173 1306 1765 1974 1173
Size: 133 459 209 2789
Fragments arranged by size:
2789 459 209 133
TatI w'GTAC_w
Cuts at: 3105 3105
Size: 3590
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 127 159 3107 3139 127
Size: 32 2948 32 578
Fragments arranged by size:
2948 578 32 32
Tth111I GACn'n_nGTC
Cuts at: 1387 1387
Size: 3590
XbaI T'CTAG_A
Cuts at: 621 621
Size: 3590
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 415 1085 415
Size: 670 2920
XmaI C'CCGG_G
Cuts at: 610 610
Size: 3590
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PmlI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI
BpmI BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI