Restriction map for
pF1KB6805
(Circular) (Six-base) MAPSORT of: pF1KB6805.seq Check: 1553 from: 1 to: 3590
pF1KB6805 3590 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 628 628
Size: 3590
Acc65I G'GTAC_C
Cuts at: 606 606
Size: 3590
AclI AA'CG_TT
Cuts at: 3430 3430
Size: 3590
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1415 1847 2594 2862 1415
Size: 432 747 268 2143
Fragments arranged by size:
2143 747 432 268
AflIII A'CryG_T
Cuts at: 942 2046 3023 942
Size: 1104 977 1509
Fragments arranged by size:
1509 1104 977
AgeI A'CCGG_T
Cuts at: 1978 1978
Size: 3590
AleI CACnn'nnGTG
Cuts at: 508 508
Size: 3590
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 228 260 228
Size: 32 3558
AlwNI CAG_nnn'CTG
Cuts at: 895 2462 895
Size: 1567 2023
ApaLI G'TGCA_C
Cuts at: 2360 2360
Size: 3590
ApoI r'AATT_y
Cuts at: 594 2943 3199 594
Size: 2349 256 985
Fragments arranged by size:
2349 985 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3590
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3590
AvaI C'yCGr_G
Cuts at: 610 610
Size: 3590
BamHI G'GATC_C
Cuts at: 615 615
Size: 3590
BanI G'GyrC_C
Cuts at: 606 1020 1268 1303 606
Size: 414 248 35 2893
Fragments arranged by size:
2893 414 248 35
BanII G_rGCy'C
Cuts at: 604 1634 604
Size: 1030 2560
BbeI G_GCGC'C
Cuts at: 1024 1272 1024
Size: 248 3342
BbsI GAAGACnn'nnnn_
Cuts at: 461 461
Size: 3590
BciVI GTATCCnnnnn_n'
Cuts at: 1488 2249 3590 1488
Size: 761 1341 1488
Fragments arranged by size:
1488 1341 761
BclI T'GATC_A
Cuts at: 1110 1110
Size: 3590
BglII A'GATC_T
Cuts at: 1105 3142 1105
Size: 2037 1553
BlpI GC'TnA_GC
Cuts at: 702 702
Size: 3590
Bme1580I G_kGCm'C
Cuts at: 222 1215 1308 2364 222
Size: 993 93 1056 1448
Fragments arranged by size:
1448 1056 993 93
BmrI ACTGGGnnnn_n'
Cuts at: 802 883 1218 3362 802
Size: 81 335 2144 1030
Fragments arranged by size:
2144 1030 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 339 1957 339
Size: 1618 1972
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 1645 2137 2435 766
Size: 879 492 298 1921
Fragments arranged by size:
1921 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 463 529 3294 463
Size: 66 2765 759
Fragments arranged by size:
2765 759 66
BsaAI yAC'GTr
Cuts at: 943 943
Size: 3590
BsaBI GATnn'nnATC
Cuts at: 6 1104 3141 6
Size: 1098 2037 455
Fragments arranged by size:
2037 1098 455
BsaHI Gr'CG_yC
Cuts at: 1021 1269 1971 3467 1021
Size: 248 702 1496 1144
Fragments arranged by size:
1496 1144 702 248
BsaWI w'CCGG_w
Cuts at: 779 1300 1978 2252 2399 2810 779
Size: 521 678 274 147 411 1559
Fragments arranged by size:
1559 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1015 1773 2350 2846 1015
Size: 758 577 496 1759
Fragments arranged by size:
1759 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1132 1178 2386 104
Size: 1028 46 1208 1308
Fragments arranged by size:
1308 1208 1028 46
BsiHKAI G_wGCw'C
Cuts at: 604 1382 1572 2364 604
Size: 778 190 792 1830
Fragments arranged by size:
1830 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 222 604 1215 1308 1382 1572 1634 2364 222
Size: 382 611 93 74 190 62 730 1448
Fragments arranged by size:
1448 730 611 382 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2810 2810
Size: 3590
BspHI T'CATG_A
Cuts at: 2766 2766
Size: 3590
BspMI ACCTGCnnnn'nnnn_
Cuts at: 481 640 854 1156 1537 481
Size: 159 214 302 381 2534
Fragments arranged by size:
2534 381 302 214 159
BsrBI CCG'CTC
Cuts at: 50 1882 1936 3420 3585 50
Size: 1832 54 1484 165 55
Fragments arranged by size:
1832 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1502 1502
Size: 3590
BsrFI r'CCGG_y
Cuts at: 1588 1769 1978 1588
Size: 181 209 3200
Fragments arranged by size:
3200 209 181
BssSI C'ACGA_G
Cuts at: 1861 2219 1861
Size: 358 3232
BstAPI GCAn_nnn'nTGC
Cuts at: 967 967
Size: 3590
BstBI TT'CG_AA
Cuts at: 1951 1951
Size: 3590
BstXI CCAn_nnnn'nTGG
Cuts at: 546 546
Size: 3590
BstYI r'GATC_y
Cuts at: 1 255 615 1082 1105 1440 1686 2698 2784
Size: 254 360 467 23 335 246 1012 86
Cuts at: 2784 2796 3142 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 360 346 335 254 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1540 1721 1884 1540
Size: 181 163 3246
Fragments arranged by size:
3246 181 163
BtsI GCAGTG_nn'
Cuts at: 833 833
Size: 3590
DraI TTT'AAA
Cuts at: 589 589
Size: 3590
DrdI GACnn_nn'nnGTC
Cuts at: 1296 2154 1296
Size: 858 2732
EaeI y'GGCC_r
Cuts at: 1175 1349 1740 1767 3112 1175
Size: 174 391 27 1345 1653
Fragments arranged by size:
1653 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1175 1175
Size: 3590
EarI CTCTTCn'nnn_
Cuts at: 1613 1823 1613
Size: 210 3380
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1798 2108 2254 3400 1798
Size: 310 146 1146 1988
Fragments arranged by size:
1988 1146 310 146
EcoICRI GAG'CTC
Cuts at: 602 602
Size: 3590
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1415 1847 2594 2862 1415
Size: 432 747 268 2143
Fragments arranged by size:
2143 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 729 729
Size: 3590
EcoRI G'AATT_C
Cuts at: 594 594
Size: 3590
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1609 1609
Size: 3590
FspI TGC'GCA
Cuts at: 1371 1371
Size: 3590
HaeII r_GCGC'y
Cuts at: 1024 1272 2294 3273 1024
Size: 248 1022 979 1341
Fragments arranged by size:
1341 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 319 351 1432 1462 1464 1494 319
Size: 32 1081 30 2 30 2415
Fragments arranged by size:
2415 1081 32 30 30 2
HincII GTy'rAC
Cuts at: 201 629 201
Size: 428 3162
KasI G'GCGC_C
Cuts at: 1020 1268 1020
Size: 248 3342
KpnI G_GTAC'C
Cuts at: 610 610
Size: 3590
MluI A'CGCG_T
Cuts at: 3023 3023
Size: 3590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2261 2445 2261
Size: 184 3406
MscI TGG'CCA
Cuts at: 1351 1351
Size: 3590
MslI CAynn'nnrTG
Cuts at: 508 1579 1706 508
Size: 1071 127 2392
Fragments arranged by size:
2392 1071 127
MspA1I CmG'CkG
Cuts at: 508 701 1015 1375 2388 2633 508
Size: 193 314 360 1013 245 1465
Fragments arranged by size:
1465 1013 360 314 245 193
NaeI GCC'GGC
Cuts at: 1771 1771
Size: 3590
NarI GG'CG_CC
Cuts at: 1021 1269 1021
Size: 248 3342
NgoMIV G'CCGG_C
Cuts at: 1769 1769
Size: 3590
NspI r_CATG'y
Cuts at: 643 1143 2050 643
Size: 500 907 2183
Fragments arranged by size:
2183 907 500
PciI A'CATG_T
Cuts at: 2046 2046
Size: 3590
PfoI T'CCnGG_A
Cuts at: 232 232
Size: 3590
PmeI GTTT'AAAC
Cuts at: 589 589
Size: 3590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2729 2761 2729
Size: 32 3558
PstI C_TGCA'G
Cuts at: 637 1322 637
Size: 685 2905
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3590
PvuII CAG'CTG
Cuts at: 508 1015 1375 508
Size: 507 360 2723
Fragments arranged by size:
2723 507 360
RsrII CG'GwC_CG
Cuts at: 1785 1785
Size: 3590
SacI G_AGCT'C
Cuts at: 604 604
Size: 3590
SalI G'TCGA_C
Cuts at: 627 627
Size: 3590
SapI GCTCTTCn'nnn_
Cuts at: 1613 1823 1613
Size: 210 3380
SbfI CC_TGCA'GG
Cuts at: 637 637
Size: 3590
ScaI AGT'ACT
Cuts at: 3107 3107
Size: 3590
SfcI C'TryA_G
Cuts at: 33 395 464 633 1318 2311 2502 33
Size: 362 69 169 685 993 191 1121
Fragments arranged by size:
1121 993 685 362 191 169 69
SfoI GGC'GCC
Cuts at: 1022 1270 1022
Size: 248 3342
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3590
SmaI CCC'GGG
Cuts at: 612 612
Size: 3590
SmlI C'TyrA_G
Cuts at: 745 1660 2152 2414 745
Size: 915 492 262 1921
Fragments arranged by size:
1921 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3590
SphI G_CATG'C
Cuts at: 643 1143 643
Size: 500 3090
StyI C'CwwG_G
Cuts at: 724 1078 724
Size: 354 3236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1173 1306 1765 1974 1173
Size: 133 459 209 2789
Fragments arranged by size:
2789 459 209 133
TatI w'GTAC_w
Cuts at: 3105 3105
Size: 3590
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3107 3139 3107
Size: 32 3558
Tth111I GACn'n_nGTC
Cuts at: 1387 1387
Size: 3590
XbaI T'CTAG_A
Cuts at: 621 621
Size: 3590
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1085 1085
Size: 3590
XmaI C'CCGG_G
Cuts at: 610 610
Size: 3590
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI