Restriction map for
pF1KB6795
(Circular) (Six-base) MAPSORT of: pF1KB6795.seq Check: 3115 from: 1 to: 3497
pF1KB6795 3497 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 286 286
Size: 3497
AccI GT'mk_AC
Cuts at: 115 535 115
Size: 420 3077
Acc65I G'GTAC_C
Cuts at: 513 513
Size: 3497
AclI AA'CG_TT
Cuts at: 3337 3337
Size: 3497
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1322 1754 2501 2769 1322
Size: 432 747 268 2050
Fragments arranged by size:
2050 747 432 268
AflIII A'CryG_T
Cuts at: 356 849 1953 2930 356
Size: 493 1104 977 923
Fragments arranged by size:
1104 977 923 493
AgeI A'CCGG_T
Cuts at: 1885 1885
Size: 3497
AlwNI CAG_nnn'CTG
Cuts at: 217 802 2369 217
Size: 585 1567 1345
Fragments arranged by size:
1567 1345 585
ApaLI G'TGCA_C
Cuts at: 2267 2267
Size: 3497
ApoI r'AATT_y
Cuts at: 501 2850 3106 501
Size: 2349 256 892
Fragments arranged by size:
2349 892 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3497
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3497
AvaI C'yCGr_G
Cuts at: 154 517 154
Size: 363 3134
BamHI G'GATC_C
Cuts at: 522 522
Size: 3497
BanI G'GyrC_C
Cuts at: 513 927 1175 1210 513
Size: 414 248 35 2800
Fragments arranged by size:
2800 414 248 35
BanII G_rGCy'C
Cuts at: 511 1541 511
Size: 1030 2467
BbeI G_GCGC'C
Cuts at: 931 1179 931
Size: 248 3249
BciVI GTATCCnnnnn_n'
Cuts at: 1395 2156 3497 1395
Size: 761 1341 1395
Fragments arranged by size:
1395 1341 761
BclI T'GATC_A
Cuts at: 1017 1017
Size: 3497
BfrBI ATG'CAT
Cuts at: 195 195
Size: 3497
BglII A'GATC_T
Cuts at: 1012 3049 1012
Size: 2037 1460
BlpI GC'TnA_GC
Cuts at: 609 609
Size: 3497
Bme1580I G_kGCm'C
Cuts at: 376 403 1122 1215 2271 376
Size: 27 719 93 1056 1602
Fragments arranged by size:
1602 1056 719 93 27
BmrI ACTGGGnnnn_n'
Cuts at: 709 790 1125 3269 709
Size: 81 335 2144 937
Fragments arranged by size:
2144 937 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1864 1864
Size: 3497
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 1552 2044 2342 673
Size: 879 492 298 1828
Fragments arranged by size:
1828 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 250 434 3201 250
Size: 184 2767 546
Fragments arranged by size:
2767 546 184
BsaAI yAC'GTr
Cuts at: 377 850 377
Size: 473 3024
BsaBI GATnn'nnATC
Cuts at: 6 1011 3048 6
Size: 1005 2037 455
Fragments arranged by size:
2037 1005 455
BsaHI Gr'CG_yC
Cuts at: 928 1176 1878 3374 928
Size: 248 702 1496 1051
Fragments arranged by size:
1496 1051 702 248
BsaWI w'CCGG_w
Cuts at: 686 1207 1885 2159 2306 2717 686
Size: 521 678 274 147 411 1466
Fragments arranged by size:
1466 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 325 325
Size: 3497
BseYI C'CCAG_C
Cuts at: 922 1680 2257 2753 922
Size: 758 577 496 1666
Fragments arranged by size:
1666 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 440
Size: 3497
BsiEI CG_ry'CG
Cuts at: 104 1039 1085 2293 104
Size: 935 46 1208 1308
Fragments arranged by size:
1308 1208 935 46
BsiHKAI G_wGCw'C
Cuts at: 511 1289 1479 2271 511
Size: 778 190 792 1737
Fragments arranged by size:
1737 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 376 403 511 1122 1215 1289 1479 1541 2271
Size: 27 108 611 93 74 190 62 730
Cuts at: 2271 376
Size: 1602
Fragments arranged by size:
1602 730 611 190 108 93 74 62
27
BspEI T'CCGG_A
Cuts at: 2717 2717
Size: 3497
BspHI T'CATG_A
Cuts at: 2673 2673
Size: 3497
BspMI ACCTGCnnnn'nnnn_
Cuts at: 286 547 761 1063 1444 286
Size: 261 214 302 381 2339
Fragments arranged by size:
2339 381 302 261 214
BsrBI CCG'CTC
Cuts at: 50 1789 1843 3327 3492 50
Size: 1739 54 1484 165 55
Fragments arranged by size:
1739 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 206 290 1409 206
Size: 84 1119 2294
Fragments arranged by size:
2294 1119 84
BsrFI r'CCGG_y
Cuts at: 362 1495 1676 1885 362
Size: 1133 181 209 1974
Fragments arranged by size:
1974 1133 209 181
BssSI C'ACGA_G
Cuts at: 1768 2126 1768
Size: 358 3139
BstAPI GCAn_nnn'nTGC
Cuts at: 874 874
Size: 3497
BstBI TT'CG_AA
Cuts at: 1858 1858
Size: 3497
BstXI CCAn_nnnn'nTGG
Cuts at: 276 460 276
Size: 184 3313
BstYI r'GATC_y
Cuts at: 1 522 989 1012 1347 1593 2605 2691 2703
Size: 521 467 23 335 246 1012 86 12
Cuts at: 2703 3049 1
Size: 346 449
Fragments arranged by size:
1012 521 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1447 1628 1791 1447
Size: 181 163 3153
Fragments arranged by size:
3153 181 163
BtsI GCAGTG_nn'
Cuts at: 740 740
Size: 3497
DraI TTT'AAA
Cuts at: 496 496
Size: 3497
DraIII CAC_nnn'GTG
Cuts at: 140 140
Size: 3497
DrdI GACnn_nn'nnGTC
Cuts at: 286 1203 2061 286
Size: 917 858 1722
Fragments arranged by size:
1722 917 858
EaeI y'GGCC_r
Cuts at: 238 314 1082 1256 1647 1674 3019 238
Size: 76 768 174 391 27 1345 716
Fragments arranged by size:
1345 768 716 391 174 76 27
EagI C'GGCC_G
Cuts at: 1082 1082
Size: 3497
EarI CTCTTCn'nnn_
Cuts at: 343 1520 1730 343
Size: 1177 210 2110
Fragments arranged by size:
2110 1177 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1705 2015 2161 3307 1705
Size: 310 146 1146 1895
Fragments arranged by size:
1895 1146 310 146
EcoICRI GAG'CTC
Cuts at: 509 509
Size: 3497
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1322 1754 2501 2769 1322
Size: 432 747 268 2050
Fragments arranged by size:
2050 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 221 221
Size: 3497
EcoO109I rG'GnC_Cy
Cuts at: 636 636
Size: 3497
EcoRI G'AATT_C
Cuts at: 501 501
Size: 3497
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 501 1516 501
Size: 1015 2482
FspI TGC'GCA
Cuts at: 1278 1278
Size: 3497
HaeII r_GCGC'y
Cuts at: 931 1179 2201 3180 931
Size: 248 1022 979 1248
Fragments arranged by size:
1248 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1339 1369 1371 1401 1339
Size: 30 2 30 3435
Fragments arranged by size:
3435 30 30 2
HincII GTy'rAC
Cuts at: 116 536 116
Size: 420 3077
KasI G'GCGC_C
Cuts at: 927 1175 927
Size: 248 3249
KpnI G_GTAC'C
Cuts at: 517 517
Size: 3497
MluI A'CGCG_T
Cuts at: 2930 2930
Size: 3497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2168 2352 2168
Size: 184 3313
MscI TGG'CCA
Cuts at: 316 1258 316
Size: 942 2555
MslI CAynn'nnrTG
Cuts at: 190 274 386 1486 1613 190
Size: 84 112 1100 127 2074
Fragments arranged by size:
2074 1100 127 112 84
MspA1I CmG'CkG
Cuts at: 608 922 1282 2295 2540 608
Size: 314 360 1013 245 1565
Fragments arranged by size:
1565 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1678 1678
Size: 3497
NarI GG'CG_CC
Cuts at: 928 1176 928
Size: 248 3249
NgoMIV G'CCGG_C
Cuts at: 1676 1676
Size: 3497
NsiI A_TGCA'T
Cuts at: 197 197
Size: 3497
NspI r_CATG'y
Cuts at: 360 550 1050 1957 360
Size: 190 500 907 1900
Fragments arranged by size:
1900 907 500 190
PciI A'CATG_T
Cuts at: 356 1953 356
Size: 1597 1900
PmeI GTTT'AAAC
Cuts at: 496 496
Size: 3497
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2636 2668 2636
Size: 32 3465
PspXI vC'TCGA_Gb
Cuts at: 154 154
Size: 3497
PstI C_TGCA'G
Cuts at: 437 544 1229 437
Size: 107 685 2705
Fragments arranged by size:
2705 685 107
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3497
PvuII CAG'CTG
Cuts at: 922 1282 922
Size: 360 3137
RsrII CG'GwC_CG
Cuts at: 1692 1692
Size: 3497
SacI G_AGCT'C
Cuts at: 511 511
Size: 3497
SalI G'TCGA_C
Cuts at: 114 534 114
Size: 420 3077
SapI GCTCTTCn'nnn_
Cuts at: 1520 1730 1520
Size: 210 3287
SbfI CC_TGCA'GG
Cuts at: 437 544 437
Size: 107 3390
ScaI AGT'ACT
Cuts at: 484 3014 484
Size: 2530 967
SfcI C'TryA_G
Cuts at: 33 433 540 1225 2218 2409 33
Size: 400 107 685 993 191 1121
Fragments arranged by size:
1121 993 685 400 191 107
SfoI GGC'GCC
Cuts at: 929 1177 929
Size: 248 3249
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3497
SmaI CCC'GGG
Cuts at: 519 519
Size: 3497
SmlI C'TyrA_G
Cuts at: 154 652 1567 2059 2321 154
Size: 498 915 492 262 1330
Fragments arranged by size:
1330 915 498 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3497
SphI G_CATG'C
Cuts at: 550 1050 550
Size: 500 2997
StyI C'CwwG_G
Cuts at: 395 631 985 395
Size: 236 354 2907
Fragments arranged by size:
2907 354 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1080 1213 1672 1881 1080
Size: 133 459 209 2696
Fragments arranged by size:
2696 459 209 133
TatI w'GTAC_w
Cuts at: 482 3012 482
Size: 2530 967
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3014 3046 3014
Size: 32 3465
Tth111I GACn'n_nGTC
Cuts at: 283 1294 283
Size: 1011 2486
XbaI T'CTAG_A
Cuts at: 528 528
Size: 3497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 992 992
Size: 3497
XhoI C'TCGA_G
Cuts at: 154 154
Size: 3497
XmaI C'CCGG_G
Cuts at: 517 517
Size: 3497
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NsiI NspI PciI
PmeI PpiI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI