Restriction map for
pF1KB6790
(Circular) (Six-base) MAPSORT of: pF1KB6790.seq Check: 1702 from: 1 to: 3578
pF1KB6790 3578 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 616 616
Size: 3578
Acc65I G'GTAC_C
Cuts at: 594 594
Size: 3578
AclI AA'CG_TT
Cuts at: 3418 3418
Size: 3578
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 1403 1835 2582 2850 134
Size: 1269 432 747 268 862
Fragments arranged by size:
1269 862 747 432 268
AflIII A'CryG_T
Cuts at: 930 2034 3011 930
Size: 1104 977 1497
Fragments arranged by size:
1497 1104 977
AgeI A'CCGG_T
Cuts at: 1966 1966
Size: 3578
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 308 308
Size: 3578
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 388 420 388
Size: 32 3546
AlwNI CAG_nnn'CTG
Cuts at: 883 2450 883
Size: 1567 2011
ApaLI G'TGCA_C
Cuts at: 2348 2348
Size: 3578
ApoI r'AATT_y
Cuts at: 582 2931 3187 582
Size: 2349 256 973
Fragments arranged by size:
2349 973 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3578
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3578
AvaI C'yCGr_G
Cuts at: 598 598
Size: 3578
BamHI G'GATC_C
Cuts at: 224 603 224
Size: 379 3199
BanI G'GyrC_C
Cuts at: 594 1008 1256 1291 594
Size: 414 248 35 2881
Fragments arranged by size:
2881 414 248 35
BanII G_rGCy'C
Cuts at: 592 1622 592
Size: 1030 2548
BbeI G_GCGC'C
Cuts at: 1012 1260 1012
Size: 248 3330
BciVI GTATCCnnnnn_n'
Cuts at: 1476 2237 3578 1476
Size: 761 1341 1476
Fragments arranged by size:
1476 1341 761
BclI T'GATC_A
Cuts at: 1098 1098
Size: 3578
BglII A'GATC_T
Cuts at: 1093 3130 1093
Size: 2037 1541
BlpI GC'TnA_GC
Cuts at: 690 690
Size: 3578
Bme1580I G_kGCm'C
Cuts at: 1203 1296 2352 1203
Size: 93 1056 2429
Fragments arranged by size:
2429 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 790 871 1206 3350 790
Size: 81 335 2144 1018
Fragments arranged by size:
2144 1018 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1945 1945
Size: 3578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 439 149
Size: 290 3288
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 754 1633 2125 2423 469
Size: 285 879 492 298 1624
Fragments arranged by size:
1624 879 492 298 285
BsaI GGTCTCn'nnnn_
Cuts at: 3282 3282
Size: 3578
BsaAI yAC'GTr
Cuts at: 931 931
Size: 3578
BsaBI GATnn'nnATC
Cuts at: 6 1092 3129 6
Size: 1086 2037 455
Fragments arranged by size:
2037 1086 455
BsaHI Gr'CG_yC
Cuts at: 1009 1257 1959 3455 1009
Size: 248 702 1496 1132
Fragments arranged by size:
1496 1132 702 248
BsaWI w'CCGG_w
Cuts at: 231 767 1288 1966 2240 2387 2798 231
Size: 536 521 678 274 147 411 1011
Fragments arranged by size:
1011 678 536 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 436 181
Size: 255 3323
BseYI C'CCAG_C
Cuts at: 1003 1761 2338 2834 1003
Size: 758 577 496 1747
Fragments arranged by size:
1747 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1120 1166 2374 104
Size: 1016 46 1208 1308
Fragments arranged by size:
1308 1208 1016 46
BsiHKAI G_wGCw'C
Cuts at: 592 1370 1560 2352 592
Size: 778 190 792 1818
Fragments arranged by size:
1818 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 592 1203 1296 1370 1560 1622 2352 592
Size: 611 93 74 190 62 730 1818
Fragments arranged by size:
1818 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 231 2798 231
Size: 2567 1011
BspHI T'CATG_A
Cuts at: 2754 2754
Size: 3578
BspMI ACCTGCnnnn'nnnn_
Cuts at: 628 842 1144 1525 628
Size: 214 302 381 2681
Fragments arranged by size:
2681 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1870 1924 3408 3573 50
Size: 1820 54 1484 165 55
Fragments arranged by size:
1820 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1490 1490
Size: 3578
BsrFI r'CCGG_y
Cuts at: 1576 1757 1966 1576
Size: 181 209 3188
Fragments arranged by size:
3188 209 181
BssSI C'ACGA_G
Cuts at: 1849 2207 1849
Size: 358 3220
BstAPI GCAn_nnn'nTGC
Cuts at: 955 955
Size: 3578
BstBI TT'CG_AA
Cuts at: 1939 1939
Size: 3578
BstYI r'GATC_y
Cuts at: 1 224 603 1070 1093 1428 1674 2686 2772
Size: 223 379 467 23 335 246 1012 86
Cuts at: 2772 2784 3130 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 379 346 335 246 223
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 220 220
Size: 3578
BtgI C'CryG_G
Cuts at: 107 350 107
Size: 243 3335
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1528 1709 1872 1528
Size: 181 163 3234
Fragments arranged by size:
3234 181 163
BtsI GCAGTG_nn'
Cuts at: 821 821
Size: 3578
DraI TTT'AAA
Cuts at: 577 577
Size: 3578
DrdI GACnn_nn'nnGTC
Cuts at: 1284 2142 1284
Size: 858 2720
EaeI y'GGCC_r
Cuts at: 336 347 528 1163 1337 1728 1755 3100 336
Size: 11 181 635 174 391 27 1345 814
Fragments arranged by size:
1345 814 635 391 181 174 27 11
EagI C'GGCC_G
Cuts at: 1163 1163
Size: 3578
EarI CTCTTCn'nnn_
Cuts at: 570 1601 1811 570
Size: 1031 210 2337
Fragments arranged by size:
2337 1031 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1786 2096 2242 3388 1786
Size: 310 146 1146 1976
Fragments arranged by size:
1976 1146 310 146
EcoICRI GAG'CTC
Cuts at: 590 590
Size: 3578
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 149 439 1403 1835 2582 2850 134
Size: 15 290 964 432 747 268 862
Fragments arranged by size:
964 862 747 432 290 268 15
EcoO109I rG'GnC_Cy
Cuts at: 717 717
Size: 3578
EcoRI G'AATT_C
Cuts at: 582 582
Size: 3578
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1597 1597
Size: 3578
FspI TGC'GCA
Cuts at: 1359 1359
Size: 3578
HaeII r_GCGC'y
Cuts at: 1012 1260 2282 3261 1012
Size: 248 1022 979 1329
Fragments arranged by size:
1329 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1420 1450 1452 1482 1420
Size: 30 2 30 3516
Fragments arranged by size:
3516 30 30 2
HincII GTy'rAC
Cuts at: 617 617
Size: 3578
KasI G'GCGC_C
Cuts at: 1008 1256 1008
Size: 248 3330
KpnI G_GTAC'C
Cuts at: 598 598
Size: 3578
MluI A'CGCG_T
Cuts at: 3011 3011
Size: 3578
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2249 2433 2249
Size: 184 3394
MscI TGG'CCA
Cuts at: 338 349 530 1339 338
Size: 11 181 809 2577
Fragments arranged by size:
2577 809 181 11
MslI CAynn'nnrTG
Cuts at: 1567 1694 1567
Size: 127 3451
MspA1I CmG'CkG
Cuts at: 282 689 1003 1363 2376 2621 282
Size: 407 314 360 1013 245 1239
Fragments arranged by size:
1239 1013 407 360 314 245
NaeI GCC'GGC
Cuts at: 1759 1759
Size: 3578
NarI GG'CG_CC
Cuts at: 1009 1257 1009
Size: 248 3330
NcoI C'CATG_G
Cuts at: 107 350 107
Size: 243 3335
NgoMIV G'CCGG_C
Cuts at: 1757 1757
Size: 3578
NspI r_CATG'y
Cuts at: 460 631 1131 2038 460
Size: 171 500 907 2000
Fragments arranged by size:
2000 907 500 171
PciI A'CATG_T
Cuts at: 2034 2034
Size: 3578
PflMI CCAn_nnn'nTGG
Cuts at: 345 345
Size: 3578
PfoI T'CCnGG_A
Cuts at: 407 407
Size: 3578
PmeI GTTT'AAAC
Cuts at: 577 577
Size: 3578
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2717 2749 2717
Size: 32 3546
PstI C_TGCA'G
Cuts at: 287 625 1310 287
Size: 338 685 2555
Fragments arranged by size:
2555 685 338
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3578
PvuII CAG'CTG
Cuts at: 282 1003 1363 282
Size: 721 360 2497
Fragments arranged by size:
2497 721 360
RsrII CG'GwC_CG
Cuts at: 1773 1773
Size: 3578
SacI G_AGCT'C
Cuts at: 592 592
Size: 3578
SalI G'TCGA_C
Cuts at: 615 615
Size: 3578
SapI GCTCTTCn'nnn_
Cuts at: 1601 1811 1601
Size: 210 3368
SbfI CC_TGCA'GG
Cuts at: 625 625
Size: 3578
ScaI AGT'ACT
Cuts at: 343 3095 343
Size: 2752 826
SfcI C'TryA_G
Cuts at: 33 198 283 621 1306 2299 2490 33
Size: 165 85 338 685 993 191 1121
Fragments arranged by size:
1121 993 685 338 191 165 85
SfoI GGC'GCC
Cuts at: 1010 1258 1010
Size: 248 3330
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3578
SmaI CCC'GGG
Cuts at: 600 600
Size: 3578
SmlI C'TyrA_G
Cuts at: 484 733 1648 2140 2402 484
Size: 249 915 492 262 1660
Fragments arranged by size:
1660 915 492 262 249
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3578
SphI G_CATG'C
Cuts at: 460 631 1131 460
Size: 171 500 2907
Fragments arranged by size:
2907 500 171
StyI C'CwwG_G
Cuts at: 107 350 712 1066 107
Size: 243 362 354 2619
Fragments arranged by size:
2619 362 354 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 160 1161 1294 1753 1962 160
Size: 1001 133 459 209 1776
Fragments arranged by size:
1776 1001 459 209 133
TatI w'GTAC_w
Cuts at: 341 3093 341
Size: 2752 826
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3095 3127 3095
Size: 32 3546
Tth111I GACn'n_nGTC
Cuts at: 1375 1375
Size: 3578
XbaI T'CTAG_A
Cuts at: 609 609
Size: 3578
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1073 1073
Size: 3578
XmaI C'CCGG_G
Cuts at: 598 598
Size: 3578
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI