Restriction map for
pF1KB6769
(Circular) (Six-base) MAPSORT of: pF1KB6769.seq Check: 7511 from: 1 to: 3461
pF1KB6769 3461 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 499 499
Size: 3461
Acc65I G'GTAC_C
Cuts at: 477 477
Size: 3461
AclI AA'CG_TT
Cuts at: 3301 3301
Size: 3461
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 1286 1718 2465 2733 394
Size: 892 432 747 268 1122
Fragments arranged by size:
1122 892 747 432 268
AflIII A'CryG_T
Cuts at: 813 1917 2894 813
Size: 1104 977 1380
Fragments arranged by size:
1380 1104 977
AgeI A'CCGG_T
Cuts at: 1849 1849
Size: 3461
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 154 154
Size: 3461
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 308 313 340 345 308
Size: 5 27 5 3424
Fragments arranged by size:
3424 27 5 5
AlwNI CAG_nnn'CTG
Cuts at: 396 766 2333 396
Size: 370 1567 1524
Fragments arranged by size:
1567 1524 370
ApaLI G'TGCA_C
Cuts at: 2231 2231
Size: 3461
ApoI r'AATT_y
Cuts at: 465 2814 3070 465
Size: 2349 256 856
Fragments arranged by size:
2349 856 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3461
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3461
AvaI C'yCGr_G
Cuts at: 254 481 254
Size: 227 3234
BamHI G'GATC_C
Cuts at: 486 486
Size: 3461
BanI G'GyrC_C
Cuts at: 477 891 1139 1174 477
Size: 414 248 35 2764
Fragments arranged by size:
2764 414 248 35
BanII G_rGCy'C
Cuts at: 196 261 475 1505 196
Size: 65 214 1030 2152
Fragments arranged by size:
2152 1030 214 65
BbeI G_GCGC'C
Cuts at: 895 1143 895
Size: 248 3213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3427
BciVI GTATCCnnnnn_n'
Cuts at: 1359 2120 3461 1359
Size: 761 1341 1359
Fragments arranged by size:
1359 1341 761
BclI T'GATC_A
Cuts at: 981 981
Size: 3461
BglI GCCn_nnn'nGGC
Cuts at: 439 439
Size: 3461
BglII A'GATC_T
Cuts at: 976 3013 976
Size: 2037 1424
BlpI GC'TnA_GC
Cuts at: 573 573
Size: 3461
Bme1580I G_kGCm'C
Cuts at: 1086 1179 2235 1086
Size: 93 1056 2312
Fragments arranged by size:
2312 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 673 754 1089 3233 673
Size: 81 335 2144 901
Fragments arranged by size:
2144 901 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1828 1828
Size: 3461
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 165
Size: 3461
Bpu10I CC'TnA_GC
Cuts at: 190 190
Size: 3461
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 637 1516 2008 2306 637
Size: 879 492 298 1792
Fragments arranged by size:
1792 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3165 3165
Size: 3461
BsaAI yAC'GTr
Cuts at: 814 814
Size: 3461
BsaBI GATnn'nnATC
Cuts at: 6 975 3012 6
Size: 969 2037 455
Fragments arranged by size:
2037 969 455
BsaHI Gr'CG_yC
Cuts at: 892 1140 1842 3338 892
Size: 248 702 1496 1015
Fragments arranged by size:
1496 1015 702 248
BsaWI w'CCGG_w
Cuts at: 326 650 1171 1849 2123 2270 2681 326
Size: 324 521 678 274 147 411 1106
Fragments arranged by size:
1106 678 521 411 324 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 133
Size: 3461
BseYI C'CCAG_C
Cuts at: 444 886 1644 2221 2717 444
Size: 442 758 577 496 1188
Fragments arranged by size:
1188 758 577 496 442
BsiEI CG_ry'CG
Cuts at: 104 1003 1049 2257 104
Size: 899 46 1208 1308
Fragments arranged by size:
1308 1208 899 46
BsiHKAI G_wGCw'C
Cuts at: 261 475 1253 1443 2235 261
Size: 214 778 190 792 1487
Fragments arranged by size:
1487 792 778 214 190
Bsp1286I G_dGCh'C
Cuts at: 196 261 475 1086 1179 1253 1443 1505 2235
Size: 65 214 611 93 74 190 62 730
Cuts at: 2235 196
Size: 1422
Fragments arranged by size:
1422 730 611 214 190 93 74 65
62
BspEI T'CCGG_A
Cuts at: 2681 2681
Size: 3461
BspHI T'CATG_A
Cuts at: 2637 2637
Size: 3461
BspMI ACCTGCnnnn'nnnn_
Cuts at: 511 725 1027 1408 511
Size: 214 302 381 2564
Fragments arranged by size:
2564 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1753 1807 3291 3456 50
Size: 1703 54 1484 165 55
Fragments arranged by size:
1703 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1373 1373
Size: 3461
BsrFI r'CCGG_y
Cuts at: 1459 1640 1849 1459
Size: 181 209 3071
Fragments arranged by size:
3071 209 181
BssSI C'ACGA_G
Cuts at: 1732 2090 1732
Size: 358 3103
BstAPI GCAn_nnn'nTGC
Cuts at: 838 838
Size: 3461
BstBI TT'CG_AA
Cuts at: 1822 1822
Size: 3461
BstYI r'GATC_y
Cuts at: 1 486 953 976 1311 1557 2569 2655 2667
Size: 485 467 23 335 246 1012 86 12
Cuts at: 2667 3013 1
Size: 346 449
Fragments arranged by size:
1012 485 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 170 170
Size: 3461
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1411 1592 1755 1411
Size: 181 163 3117
Fragments arranged by size:
3117 181 163
BtsI GCAGTG_nn'
Cuts at: 704 704
Size: 3461
DraI TTT'AAA
Cuts at: 460 460
Size: 3461
DrdI GACnn_nn'nnGTC
Cuts at: 1167 2025 1167
Size: 858 2603
EaeI y'GGCC_r
Cuts at: 167 1046 1220 1611 1638 2983 167
Size: 879 174 391 27 1345 645
Fragments arranged by size:
1345 879 645 391 174 27
EagI C'GGCC_G
Cuts at: 1046 1046
Size: 3461
EarI CTCTTCn'nnn_
Cuts at: 422 1484 1694 422
Size: 1062 210 2189
Fragments arranged by size:
2189 1062 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 265 1669 1979 2125 3271 265
Size: 1404 310 146 1146 455
Fragments arranged by size:
1404 1146 455 310 146
EcoICRI GAG'CTC
Cuts at: 259 473 259
Size: 214 3247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 394 1286 1718 2465 2733 165
Size: 229 892 432 747 268 893
Fragments arranged by size:
893 892 747 432 268 229
EcoO109I rG'GnC_Cy
Cuts at: 393 441 600 393
Size: 48 159 3254
Fragments arranged by size:
3254 159 48
EcoRI G'AATT_C
Cuts at: 465 465
Size: 3461
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1480 1480
Size: 3461
FspI TGC'GCA
Cuts at: 1242 1242
Size: 3461
HaeII r_GCGC'y
Cuts at: 895 1143 2165 3144 895
Size: 248 1022 979 1212
Fragments arranged by size:
1212 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1303 1333 1335 1365 1303
Size: 30 2 30 3399
Fragments arranged by size:
3399 30 30 2
HincII GTy'rAC
Cuts at: 500 500
Size: 3461
KasI G'GCGC_C
Cuts at: 891 1139 891
Size: 248 3213
KpnI G_GTAC'C
Cuts at: 481 481
Size: 3461
MluI A'CGCG_T
Cuts at: 2894 2894
Size: 3461
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 330 2132 2316 330
Size: 1802 184 1475
Fragments arranged by size:
1802 1475 184
MscI TGG'CCA
Cuts at: 169 1222 169
Size: 1053 2408
MslI CAynn'nnrTG
Cuts at: 1450 1577 1450
Size: 127 3334
MspA1I CmG'CkG
Cuts at: 572 886 1246 2259 2504 572
Size: 314 360 1013 245 1529
Fragments arranged by size:
1529 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1642 1642
Size: 3461
NarI GG'CG_CC
Cuts at: 892 1140 892
Size: 248 3213
NcoI C'CATG_G
Cuts at: 170 170
Size: 3461
NgoMIV G'CCGG_C
Cuts at: 1640 1640
Size: 3461
NspI r_CATG'y
Cuts at: 153 514 1014 1921 153
Size: 361 500 907 1693
Fragments arranged by size:
1693 907 500 361
PasI CC'CwG_GG
Cuts at: 118 396 118
Size: 278 3183
PciI A'CATG_T
Cuts at: 1917 1917
Size: 3461
PflMI CCAn_nnn'nTGG
Cuts at: 396 396
Size: 3461
PmeI GTTT'AAAC
Cuts at: 460 460
Size: 3461
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2600 2632 2600
Size: 32 3429
PstI C_TGCA'G
Cuts at: 133 251 508 1193 133
Size: 118 257 685 2401
Fragments arranged by size:
2401 685 257 118
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3461
PvuII CAG'CTG
Cuts at: 886 1246 886
Size: 360 3101
RsrII CG'GwC_CG
Cuts at: 1656 1656
Size: 3461
SacI G_AGCT'C
Cuts at: 261 475 261
Size: 214 3247
SalI G'TCGA_C
Cuts at: 498 498
Size: 3461
SapI GCTCTTCn'nnn_
Cuts at: 1484 1694 1484
Size: 210 3251
SbfI CC_TGCA'GG
Cuts at: 508 508
Size: 3461
ScaI AGT'ACT
Cuts at: 2978 2978
Size: 3461
SfcI C'TryA_G
Cuts at: 33 129 247 504 1189 2182 2373 33
Size: 96 118 257 685 993 191 1121
Fragments arranged by size:
1121 993 685 257 191 118 96
SfiI GGCCn_nnn'nGGCC
Cuts at: 439 439
Size: 3461
SfoI GGC'GCC
Cuts at: 893 1141 893
Size: 248 3213
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3461
SmaI CCC'GGG
Cuts at: 483 483
Size: 3461
SmlI C'TyrA_G
Cuts at: 616 1531 2023 2285 616
Size: 915 492 262 1792
Fragments arranged by size:
1792 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3461
SphI G_CATG'C
Cuts at: 153 514 1014 153
Size: 361 500 2600
Fragments arranged by size:
2600 500 361
StyI C'CwwG_G
Cuts at: 170 595 949 170
Size: 425 354 2682
Fragments arranged by size:
2682 425 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1044 1177 1636 1845 1044
Size: 133 459 209 2660
Fragments arranged by size:
2660 459 209 133
TatI w'GTAC_w
Cuts at: 2976 2976
Size: 3461
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2978 3010 2978
Size: 32 3429
Tth111I GACn'n_nGTC
Cuts at: 1258 1258
Size: 3461
XbaI T'CTAG_A
Cuts at: 492 492
Size: 3461
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 956 956
Size: 3461
XmaI C'CCGG_G
Cuts at: 481 481
Size: 3461
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI