Restriction map for
pF1KB6768
(Circular) (Six-base) MAPSORT of: pF1KB6768.seq Check: 4214 from: 1 to: 3557
pF1KB6768 3557 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 595 595
Size: 3557
Acc65I G'GTAC_C
Cuts at: 573 573
Size: 3557
AclI AA'CG_TT
Cuts at: 3397 3397
Size: 3557
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1382 1814 2561 2829 1382
Size: 432 747 268 2110
Fragments arranged by size:
2110 747 432 268
AflIII A'CryG_T
Cuts at: 909 2013 2990 909
Size: 1104 977 1476
Fragments arranged by size:
1476 1104 977
AgeI A'CCGG_T
Cuts at: 1945 1945
Size: 3557
AleI CACnn'nnGTG
Cuts at: 283 283
Size: 3557
AlwNI CAG_nnn'CTG
Cuts at: 862 2429 862
Size: 1567 1990
ApaLI G'TGCA_C
Cuts at: 2327 2327
Size: 3557
ApoI r'AATT_y
Cuts at: 358 561 2910 3166 358
Size: 203 2349 256 749
Fragments arranged by size:
2349 749 256 203
AseI AT'TA_AT
Cuts at: 20 20
Size: 3557
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3557
AvaI C'yCGr_G
Cuts at: 170 213 577 170
Size: 43 364 3150
Fragments arranged by size:
3150 364 43
BamHI G'GATC_C
Cuts at: 582 582
Size: 3557
BanI G'GyrC_C
Cuts at: 277 573 987 1235 1270 277
Size: 296 414 248 35 2564
Fragments arranged by size:
2564 414 296 248 35
BanII G_rGCy'C
Cuts at: 274 571 1601 274
Size: 297 1030 2230
Fragments arranged by size:
2230 1030 297
BbeI G_GCGC'C
Cuts at: 991 1239 991
Size: 248 3309
BciVI GTATCCnnnnn_n'
Cuts at: 1455 2216 3557 1455
Size: 761 1341 1455
Fragments arranged by size:
1455 1341 761
BclI T'GATC_A
Cuts at: 197 1077 197
Size: 880 2677
BglII A'GATC_T
Cuts at: 1072 3109 1072
Size: 2037 1520
BlpI GC'TnA_GC
Cuts at: 669 669
Size: 3557
Bme1580I G_kGCm'C
Cuts at: 1182 1275 2331 1182
Size: 93 1056 2408
Fragments arranged by size:
2408 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 769 850 1185 3329 769
Size: 81 335 2144 997
Fragments arranged by size:
2144 997 335 81
BmtI G_CTAG'C
Cuts at: 127 127
Size: 3557
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1924 1924
Size: 3557
Bpu10I CC'TnA_GC
Cuts at: 273 273
Size: 3557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 1612 2104 2402 733
Size: 879 492 298 1888
Fragments arranged by size:
1888 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3261 3261
Size: 3557
BsaAI yAC'GTr
Cuts at: 910 910
Size: 3557
BsaBI GATnn'nnATC
Cuts at: 6 1071 3108 6
Size: 1065 2037 455
Fragments arranged by size:
2037 1065 455
BsaHI Gr'CG_yC
Cuts at: 988 1236 1938 3434 988
Size: 248 702 1496 1111
Fragments arranged by size:
1496 1111 702 248
BsaWI w'CCGG_w
Cuts at: 746 1267 1945 2219 2366 2777 746
Size: 521 678 274 147 411 1526
Fragments arranged by size:
1526 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 982 1740 2317 2813 982
Size: 758 577 496 1726
Fragments arranged by size:
1726 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 471 1099 1145 2353 104
Size: 367 628 46 1208 1308
Fragments arranged by size:
1308 1208 628 367 46
BsiHKAI G_wGCw'C
Cuts at: 571 1349 1539 2331 571
Size: 778 190 792 1797
Fragments arranged by size:
1797 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 274 571 1182 1275 1349 1539 1601 2331 274
Size: 297 611 93 74 190 62 730 1500
Fragments arranged by size:
1500 730 611 297 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2777 2777
Size: 3557
BspHI T'CATG_A
Cuts at: 2733 2733
Size: 3557
BspMI ACCTGCnnnn'nnnn_
Cuts at: 607 821 1123 1504 607
Size: 214 302 381 2660
Fragments arranged by size:
2660 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1849 1903 3387 3552 50
Size: 1799 54 1484 165 55
Fragments arranged by size:
1799 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1469 1469
Size: 3557
BsrFI r'CCGG_y
Cuts at: 1555 1736 1945 1555
Size: 181 209 3167
Fragments arranged by size:
3167 209 181
BssSI C'ACGA_G
Cuts at: 1828 2186 1828
Size: 358 3199
BstAPI GCAn_nnn'nTGC
Cuts at: 934 934
Size: 3557
BstBI TT'CG_AA
Cuts at: 1918 1918
Size: 3557
BstXI CCAn_nnnn'nTGG
Cuts at: 345 345
Size: 3557
BstYI r'GATC_y
Cuts at: 1 582 1049 1072 1407 1653 2665 2751 2763
Size: 581 467 23 335 246 1012 86 12
Cuts at: 2763 3109 1
Size: 346 449
Fragments arranged by size:
1012 581 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 284 284
Size: 3557
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1507 1688 1851 1507
Size: 181 163 3213
Fragments arranged by size:
3213 181 163
BtsI GCAGTG_nn'
Cuts at: 800 800
Size: 3557
DraI TTT'AAA
Cuts at: 556 556
Size: 3557
DrdI GACnn_nn'nnGTC
Cuts at: 1263 2121 1263
Size: 858 2699
EaeI y'GGCC_r
Cuts at: 143 1142 1316 1707 1734 3079 143
Size: 999 174 391 27 1345 621
Fragments arranged by size:
1345 999 621 391 174 27
EagI C'GGCC_G
Cuts at: 1142 1142
Size: 3557
EarI CTCTTCn'nnn_
Cuts at: 1580 1790 1580
Size: 210 3347
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1765 2075 2221 3367 1765
Size: 310 146 1146 1955
Fragments arranged by size:
1955 1146 310 146
EcoICRI GAG'CTC
Cuts at: 569 569
Size: 3557
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1382 1814 2561 2829 1382
Size: 432 747 268 2110
Fragments arranged by size:
2110 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 696 696
Size: 3557
EcoRI G'AATT_C
Cuts at: 561 561
Size: 3557
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1576 1576
Size: 3557
FspI TGC'GCA
Cuts at: 1338 1338
Size: 3557
HaeII r_GCGC'y
Cuts at: 991 1239 2261 3240 991
Size: 248 1022 979 1308
Fragments arranged by size:
1308 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1399 1429 1431 1461 1399
Size: 30 2 30 3495
Fragments arranged by size:
3495 30 30 2
HincII GTy'rAC
Cuts at: 596 596
Size: 3557
KasI G'GCGC_C
Cuts at: 987 1235 987
Size: 248 3309
KpnI G_GTAC'C
Cuts at: 577 577
Size: 3557
MluI A'CGCG_T
Cuts at: 2990 2990
Size: 3557
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2228 2412 2228
Size: 184 3373
MscI TGG'CCA
Cuts at: 1318 1318
Size: 3557
MslI CAynn'nnrTG
Cuts at: 283 399 1546 1673 283
Size: 116 1147 127 2167
Fragments arranged by size:
2167 1147 127 116
MspA1I CmG'CkG
Cuts at: 158 353 668 982 1342 2355 2600 158
Size: 195 315 314 360 1013 245 1115
Fragments arranged by size:
1115 1013 360 315 314 245 195
NaeI GCC'GGC
Cuts at: 1738 1738
Size: 3557
NarI GG'CG_CC
Cuts at: 988 1236 988
Size: 248 3309
NgoMIV G'CCGG_C
Cuts at: 1736 1736
Size: 3557
NheI G'CTAG_C
Cuts at: 123 123
Size: 3557
NspI r_CATG'y
Cuts at: 610 1110 2017 610
Size: 500 907 2150
Fragments arranged by size:
2150 907 500
PciI A'CATG_T
Cuts at: 2013 2013
Size: 3557
PmeI GTTT'AAAC
Cuts at: 556 556
Size: 3557
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2696 2728 2696
Size: 32 3525
PstI C_TGCA'G
Cuts at: 604 1289 604
Size: 685 2872
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3557
PvuII CAG'CTG
Cuts at: 353 982 1342 353
Size: 629 360 2568
Fragments arranged by size:
2568 629 360
RsrII CG'GwC_CG
Cuts at: 1752 1752
Size: 3557
SacI G_AGCT'C
Cuts at: 571 571
Size: 3557
SalI G'TCGA_C
Cuts at: 594 594
Size: 3557
SapI GCTCTTCn'nnn_
Cuts at: 1580 1790 1580
Size: 210 3347
SbfI CC_TGCA'GG
Cuts at: 604 604
Size: 3557
ScaI AGT'ACT
Cuts at: 3074 3074
Size: 3557
SfcI C'TryA_G
Cuts at: 33 423 600 1285 2278 2469 33
Size: 390 177 685 993 191 1121
Fragments arranged by size:
1121 993 685 390 191 177
SfoI GGC'GCC
Cuts at: 989 1237 989
Size: 248 3309
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3557
SmaI CCC'GGG
Cuts at: 215 579 215
Size: 364 3193
SmlI C'TyrA_G
Cuts at: 712 1627 2119 2381 712
Size: 915 492 262 1888
Fragments arranged by size:
1888 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3557
SphI G_CATG'C
Cuts at: 610 1110 610
Size: 500 3057
StuI AGG'CCT
Cuts at: 451 451
Size: 3557
StyI C'CwwG_G
Cuts at: 691 1045 691
Size: 354 3203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 397 1140 1273 1732 1941 397
Size: 743 133 459 209 2013
Fragments arranged by size:
2013 743 459 209 133
TatI w'GTAC_w
Cuts at: 3072 3072
Size: 3557
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3074 3106 3074
Size: 32 3525
Tth111I GACn'n_nGTC
Cuts at: 1354 1354
Size: 3557
XbaI T'CTAG_A
Cuts at: 588 588
Size: 3557
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1052 1052
Size: 3557
XmaI C'CCGG_G
Cuts at: 213 577 213
Size: 364 3193
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NheI NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BpmI BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI