Restriction map for
pF1KB6756
(Circular) (Six-base) MAPSORT of: pF1KB6756.seq Check: 6013 from: 1 to: 3551
pF1KB6756 3551 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 589 589
Size: 3551
Acc65I G'GTAC_C
Cuts at: 567 567
Size: 3551
AclI AA'CG_TT
Cuts at: 545 3391 545
Size: 2846 705
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1376 1808 2555 2823 1376
Size: 432 747 268 2104
Fragments arranged by size:
2104 747 432 268
AflIII A'CryG_T
Cuts at: 365 903 2007 2984 365
Size: 538 1104 977 932
Fragments arranged by size:
1104 977 932 538
AgeI A'CCGG_T
Cuts at: 1939 1939
Size: 3551
AlwNI CAG_nnn'CTG
Cuts at: 214 856 2423 214
Size: 642 1567 1342
Fragments arranged by size:
1567 1342 642
ApaLI G'TGCA_C
Cuts at: 2321 2321
Size: 3551
ApoI r'AATT_y
Cuts at: 555 2904 3160 555
Size: 2349 256 946
Fragments arranged by size:
2349 946 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3551
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3551
AvaI C'yCGr_G
Cuts at: 571 571
Size: 3551
BamHI G'GATC_C
Cuts at: 576 576
Size: 3551
BanI G'GyrC_C
Cuts at: 567 981 1229 1264 567
Size: 414 248 35 2854
Fragments arranged by size:
2854 414 248 35
BanII G_rGCy'C
Cuts at: 245 565 1595 245
Size: 320 1030 2201
Fragments arranged by size:
2201 1030 320
BbeI G_GCGC'C
Cuts at: 985 1233 985
Size: 248 3303
BbsI GAAGACnn'nnnn_
Cuts at: 397 397
Size: 3551
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 366 400 90
Size: 34 242 34 3241
Fragments arranged by size:
3241 242 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1449 2210 3551 1449
Size: 761 1341 1449
Fragments arranged by size:
1449 1341 761
BclI T'GATC_A
Cuts at: 359 1071 359
Size: 712 2839
BfrBI ATG'CAT
Cuts at: 111 111
Size: 3551
BglI GCCn_nnn'nGGC
Cuts at: 194 194
Size: 3551
BglII A'GATC_T
Cuts at: 1066 3103 1066
Size: 2037 1514
BlpI GC'TnA_GC
Cuts at: 406 663 406
Size: 257 3294
Bme1580I G_kGCm'C
Cuts at: 1176 1269 2325 1176
Size: 93 1056 2402
Fragments arranged by size:
2402 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 151 480 763 844 1179 3323 151
Size: 329 283 81 335 2144 379
Fragments arranged by size:
2144 379 335 329 283 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1918 1918
Size: 3551
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 1606 2098 2396 727
Size: 879 492 298 1882
Fragments arranged by size:
1882 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 435 3255 435
Size: 2820 731
BsaAI yAC'GTr
Cuts at: 904 904
Size: 3551
BsaBI GATnn'nnATC
Cuts at: 6 1065 3102 6
Size: 1059 2037 455
Fragments arranged by size:
2037 1059 455
BsaHI Gr'CG_yC
Cuts at: 982 1230 1932 3428 982
Size: 248 702 1496 1105
Fragments arranged by size:
1496 1105 702 248
BsaWI w'CCGG_w
Cuts at: 740 1261 1939 2213 2360 2771 740
Size: 521 678 274 147 411 1520
Fragments arranged by size:
1520 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 109 265 109
Size: 156 3395
BseYI C'CCAG_C
Cuts at: 436 976 1734 2311 2807 436
Size: 540 758 577 496 1180
Fragments arranged by size:
1180 758 577 540 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 362
Size: 3551
BsiEI CG_ry'CG
Cuts at: 104 1093 1139 2347 104
Size: 989 46 1208 1308
Fragments arranged by size:
1308 1208 989 46
BsiHKAI G_wGCw'C
Cuts at: 245 565 1343 1533 2325 245
Size: 320 778 190 792 1471
Fragments arranged by size:
1471 792 778 320 190
Bsp1286I G_dGCh'C
Cuts at: 245 565 1176 1269 1343 1533 1595 2325 245
Size: 320 611 93 74 190 62 730 1471
Fragments arranged by size:
1471 730 611 320 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2771 2771
Size: 3551
BspHI T'CATG_A
Cuts at: 2727 2727
Size: 3551
BspMI ACCTGCnnnn'nnnn_
Cuts at: 509 601 815 1117 1498 509
Size: 92 214 302 381 2562
Fragments arranged by size:
2562 381 302 214 92
BsrBI CCG'CTC
Cuts at: 50 117 288 410 1843 1897 3381 3546 50
Size: 67 171 122 1433 54 1484 165 55
Fragments arranged by size:
1484 1433 171 165 122 67 55 54
BsrDI GCAATG_nn'
Cuts at: 173 300 1463 173
Size: 127 1163 2261
Fragments arranged by size:
2261 1163 127
BsrFI r'CCGG_y
Cuts at: 1549 1730 1939 1549
Size: 181 209 3161
Fragments arranged by size:
3161 209 181
BssSI C'ACGA_G
Cuts at: 1822 2180 1822
Size: 358 3193
BstAPI GCAn_nnn'nTGC
Cuts at: 928 928
Size: 3551
BstBI TT'CG_AA
Cuts at: 1912 1912
Size: 3551
BstYI r'GATC_y
Cuts at: 1 576 1043 1066 1401 1647 2659 2745 2757
Size: 575 467 23 335 246 1012 86 12
Cuts at: 2757 3103 1
Size: 346 449
Fragments arranged by size:
1012 575 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 235 235
Size: 3551
BtgI C'CryG_G
Cuts at: 167 167
Size: 3551
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1501 1682 1845 1501
Size: 181 163 3207
Fragments arranged by size:
3207 181 163
BtsI GCAGTG_nn'
Cuts at: 384 794 384
Size: 410 3141
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 156 191 156
Size: 35 3516
DraI TTT'AAA
Cuts at: 515 550 515
Size: 35 3516
DrdI GACnn_nn'nnGTC
Cuts at: 1257 2115 1257
Size: 858 2693
EaeI y'GGCC_r
Cuts at: 1136 1310 1701 1728 3073 1136
Size: 174 391 27 1345 1614
Fragments arranged by size:
1614 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1136 1136
Size: 3551
EarI CTCTTCn'nnn_
Cuts at: 1574 1784 1574
Size: 210 3341
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1759 2069 2215 3361 1759
Size: 310 146 1146 1949
Fragments arranged by size:
1949 1146 310 146
EcoICRI GAG'CTC
Cuts at: 243 563 243
Size: 320 3231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1376 1808 2555 2823 1376
Size: 432 747 268 2104
Fragments arranged by size:
2104 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 211 498 690 211
Size: 287 192 3072
Fragments arranged by size:
3072 287 192
EcoRI G'AATT_C
Cuts at: 555 555
Size: 3551
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1570 1570
Size: 3551
FspI TGC'GCA
Cuts at: 1332 1332
Size: 3551
HaeII r_GCGC'y
Cuts at: 157 985 1233 2255 3234 157
Size: 828 248 1022 979 474
Fragments arranged by size:
1022 979 828 474 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1393 1423 1425 1455 1393
Size: 30 2 30 3489
Fragments arranged by size:
3489 30 30 2
HincII GTy'rAC
Cuts at: 164 261 590 164
Size: 97 329 3125
Fragments arranged by size:
3125 329 97
KasI G'GCGC_C
Cuts at: 981 1229 981
Size: 248 3303
KpnI G_GTAC'C
Cuts at: 571 571
Size: 3551
MluI A'CGCG_T
Cuts at: 2984 2984
Size: 3551
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2222 2406 2222
Size: 184 3367
MscI TGG'CCA
Cuts at: 1312 1312
Size: 3551
MslI CAynn'nnrTG
Cuts at: 1540 1667 1540
Size: 127 3424
MspA1I CmG'CkG
Cuts at: 328 436 662 976 1336 2349 2594 328
Size: 108 226 314 360 1013 245 1285
Fragments arranged by size:
1285 1013 360 314 245 226 108
NaeI GCC'GGC
Cuts at: 1732 1732
Size: 3551
NarI GG'CG_CC
Cuts at: 982 1230 982
Size: 248 3303
NgoMIV G'CCGG_C
Cuts at: 1730 1730
Size: 3551
NruI TCG'CGA
Cuts at: 530 530
Size: 3551
NsiI A_TGCA'T
Cuts at: 113 113
Size: 3551
NspI r_CATG'y
Cuts at: 336 460 604 1104 2011 336
Size: 124 144 500 907 1876
Fragments arranged by size:
1876 907 500 144 124
PciI A'CATG_T
Cuts at: 2007 2007
Size: 3551
PmeI GTTT'AAAC
Cuts at: 550 550
Size: 3551
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 523 555 2690 2722 523
Size: 32 2135 32 1352
Fragments arranged by size:
2135 1352 32 32
PpuMI rG'GwC_Cy
Cuts at: 498 498
Size: 3551
PshAI GACnn'nnGTC
Cuts at: 169 169
Size: 3551
PstI C_TGCA'G
Cuts at: 379 598 1283 379
Size: 219 685 2647
Fragments arranged by size:
2647 685 219
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3551
PvuII CAG'CTG
Cuts at: 328 436 976 1336 328
Size: 108 540 360 2543
Fragments arranged by size:
2543 540 360 108
RsrII CG'GwC_CG
Cuts at: 1746 1746
Size: 3551
SacI G_AGCT'C
Cuts at: 245 565 245
Size: 320 3231
SalI G'TCGA_C
Cuts at: 588 588
Size: 3551
SapI GCTCTTCn'nnn_
Cuts at: 1574 1784 1574
Size: 210 3341
SbfI CC_TGCA'GG
Cuts at: 598 598
Size: 3551
ScaI AGT'ACT
Cuts at: 3068 3068
Size: 3551
SfcI C'TryA_G
Cuts at: 33 227 375 594 1279 2272 2463 33
Size: 194 148 219 685 993 191 1121
Fragments arranged by size:
1121 993 685 219 194 191 148
SfoI GGC'GCC
Cuts at: 983 1231 983
Size: 248 3303
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3551
SmaI CCC'GGG
Cuts at: 573 573
Size: 3551
SmlI C'TyrA_G
Cuts at: 706 1621 2113 2375 706
Size: 915 492 262 1882
Fragments arranged by size:
1882 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3551
SphI G_CATG'C
Cuts at: 604 1104 604
Size: 500 3051
StyI C'CwwG_G
Cuts at: 189 685 1039 189
Size: 496 354 2701
Fragments arranged by size:
2701 496 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 283 1134 1267 1726 1935 283
Size: 851 133 459 209 1899
Fragments arranged by size:
1899 851 459 209 133
TatI w'GTAC_w
Cuts at: 335 3066 335
Size: 2731 820
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3068 3100 3068
Size: 32 3519
Tth111I GACn'n_nGTC
Cuts at: 1348 1348
Size: 3551
XbaI T'CTAG_A
Cuts at: 582 582
Size: 3551
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 477 1046 477
Size: 569 2982
XmaI C'CCGG_G
Cuts at: 571 571
Size: 3551
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NruI
NsiI NspI PciI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BmgBI BmtI BpmI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI PacI
PasI PflMI PfoI PmlI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI