Restriction map for
pF1KB6751
(Circular) (Six-base) MAPSORT of: pF1KB6751.seq Check: 9312 from: 1 to: 3425
pF1KB6751 3425 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 463 463
Size: 3425
Acc65I G'GTAC_C
Cuts at: 441 441
Size: 3425
AclI AA'CG_TT
Cuts at: 3265 3265
Size: 3425
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1250 1682 2429 2697 1250
Size: 432 747 268 1978
Fragments arranged by size:
1978 747 432 268
AfeI AGC'GCT
Cuts at: 135 135
Size: 3425
AflIII A'CryG_T
Cuts at: 311 777 1881 2858 311
Size: 466 1104 977 878
Fragments arranged by size:
1104 977 878 466
AgeI A'CCGG_T
Cuts at: 1813 1813
Size: 3425
AlwNI CAG_nnn'CTG
Cuts at: 156 730 2297 156
Size: 574 1567 1284
Fragments arranged by size:
1567 1284 574
ApaLI G'TGCA_C
Cuts at: 2195 2195
Size: 3425
ApoI r'AATT_y
Cuts at: 429 2778 3034 429
Size: 2349 256 820
Fragments arranged by size:
2349 820 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3425
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3425
AvaI C'yCGr_G
Cuts at: 445 445
Size: 3425
BamHI G'GATC_C
Cuts at: 450 450
Size: 3425
BanI G'GyrC_C
Cuts at: 441 855 1103 1138 441
Size: 414 248 35 2728
Fragments arranged by size:
2728 414 248 35
BanII G_rGCy'C
Cuts at: 439 1469 439
Size: 1030 2395
BbeI G_GCGC'C
Cuts at: 859 1107 859
Size: 248 3177
BbvCI CC'TCA_GC
Cuts at: 137 137
Size: 3425
BciVI GTATCCnnnnn_n'
Cuts at: 1323 2084 3425 1323
Size: 761 1341 1323
Fragments arranged by size:
1341 1323 761
BclI T'GATC_A
Cuts at: 945 945
Size: 3425
BglI GCCn_nnn'nGGC
Cuts at: 274 274
Size: 3425
BglII A'GATC_T
Cuts at: 940 2977 940
Size: 2037 1388
BlpI GC'TnA_GC
Cuts at: 130 337 537 130
Size: 207 200 3018
Fragments arranged by size:
3018 207 200
Bme1580I G_kGCm'C
Cuts at: 1050 1143 2199 1050
Size: 93 1056 2276
Fragments arranged by size:
2276 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 637 718 1053 3197 637
Size: 81 335 2144 865
Fragments arranged by size:
2144 865 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1792 1792
Size: 3425
Bpu10I CC'TnA_GC
Cuts at: 137 137
Size: 3425
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 1480 1972 2270 601
Size: 879 492 298 1756
Fragments arranged by size:
1756 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3129 3129
Size: 3425
BsaAI yAC'GTr
Cuts at: 778 778
Size: 3425
BsaBI GATnn'nnATC
Cuts at: 6 939 2976 6
Size: 933 2037 455
Fragments arranged by size:
2037 933 455
BsaHI Gr'CG_yC
Cuts at: 856 1104 1806 3302 856
Size: 248 702 1496 979
Fragments arranged by size:
1496 979 702 248
BsaWI w'CCGG_w
Cuts at: 614 1135 1813 2087 2234 2645 614
Size: 521 678 274 147 411 1394
Fragments arranged by size:
1394 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 154
Size: 3425
BseYI C'CCAG_C
Cuts at: 850 1608 2185 2681 850
Size: 758 577 496 1594
Fragments arranged by size:
1594 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 967 1013 2221 104
Size: 863 46 1208 1308
Fragments arranged by size:
1308 1208 863 46
BsiHKAI G_wGCw'C
Cuts at: 343 439 1217 1407 2199 343
Size: 96 778 190 792 1569
Fragments arranged by size:
1569 792 778 190 96
Bsp1286I G_dGCh'C
Cuts at: 343 439 1050 1143 1217 1407 1469 2199 343
Size: 96 611 93 74 190 62 730 1569
Fragments arranged by size:
1569 730 611 190 96 93 74 62
BspEI T'CCGG_A
Cuts at: 2645 2645
Size: 3425
BspHI T'CATG_A
Cuts at: 254 2601 254
Size: 2347 1078
BspMI ACCTGCnnnn'nnnn_
Cuts at: 475 689 991 1372 475
Size: 214 302 381 2528
Fragments arranged by size:
2528 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1717 1771 3255 3420 50
Size: 1667 54 1484 165 55
Fragments arranged by size:
1667 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1337 1337
Size: 3425
BsrFI r'CCGG_y
Cuts at: 355 1423 1604 1813 355
Size: 1068 181 209 1967
Fragments arranged by size:
1967 1068 209 181
BsrGI T'GTAC_A
Cuts at: 314 314
Size: 3425
BssSI C'ACGA_G
Cuts at: 1696 2054 1696
Size: 358 3067
BstAPI GCAn_nnn'nTGC
Cuts at: 802 802
Size: 3425
BstBI TT'CG_AA
Cuts at: 1786 1786
Size: 3425
BstYI r'GATC_y
Cuts at: 1 450 917 940 1275 1521 2533 2619 2631
Size: 449 467 23 335 246 1012 86 12
Cuts at: 2631 2977 1
Size: 346 449
Fragments arranged by size:
1012 467 449 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 166 166
Size: 3425
BtgI C'CryG_G
Cuts at: 107 107
Size: 3425
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1375 1556 1719 1375
Size: 181 163 3081
Fragments arranged by size:
3081 181 163
BtsI GCAGTG_nn'
Cuts at: 413 668 413
Size: 255 3170
DraI TTT'AAA
Cuts at: 424 424
Size: 3425
DrdI GACnn_nn'nnGTC
Cuts at: 1131 1989 1131
Size: 858 2567
EaeI y'GGCC_r
Cuts at: 383 1010 1184 1575 1602 2947 383
Size: 627 174 391 27 1345 861
Fragments arranged by size:
1345 861 627 391 174 27
EagI C'GGCC_G
Cuts at: 1010 1010
Size: 3425
EarI CTCTTCn'nnn_
Cuts at: 1448 1658 1448
Size: 210 3215
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1633 1943 2089 3235 1633
Size: 310 146 1146 1823
Fragments arranged by size:
1823 1146 310 146
EcoICRI GAG'CTC
Cuts at: 437 437
Size: 3425
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1250 1682 2429 2697 1250
Size: 432 747 268 1978
Fragments arranged by size:
1978 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 242 564 242
Size: 322 3103
EcoRI G'AATT_C
Cuts at: 429 429
Size: 3425
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1444 1444
Size: 3425
FspI TGC'GCA
Cuts at: 1206 1206
Size: 3425
HaeII r_GCGC'y
Cuts at: 137 859 1107 2129 3108 137
Size: 722 248 1022 979 454
Fragments arranged by size:
1022 979 722 454 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1267 1297 1299 1329 1267
Size: 30 2 30 3363
Fragments arranged by size:
3363 30 30 2
HincII GTy'rAC
Cuts at: 464 464
Size: 3425
KasI G'GCGC_C
Cuts at: 855 1103 855
Size: 248 3177
KpnI G_GTAC'C
Cuts at: 445 445
Size: 3425
MluI A'CGCG_T
Cuts at: 2858 2858
Size: 3425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2096 2280 2096
Size: 184 3241
MscI TGG'CCA
Cuts at: 385 1186 385
Size: 801 2624
MslI CAynn'nnrTG
Cuts at: 350 1414 1541 350
Size: 1064 127 2234
Fragments arranged by size:
2234 1064 127
MspA1I CmG'CkG
Cuts at: 153 536 850 1210 2223 2468 153
Size: 383 314 360 1013 245 1110
Fragments arranged by size:
1110 1013 383 360 314 245
NaeI GCC'GGC
Cuts at: 357 1606 357
Size: 1249 2176
NarI GG'CG_CC
Cuts at: 856 1104 856
Size: 248 3177
NcoI C'CATG_G
Cuts at: 107 107
Size: 3425
NgoMIV G'CCGG_C
Cuts at: 355 1604 355
Size: 1249 2176
NspI r_CATG'y
Cuts at: 478 978 1885 478
Size: 500 907 2018
Fragments arranged by size:
2018 907 500
PciI A'CATG_T
Cuts at: 1881 1881
Size: 3425
PmeI GTTT'AAAC
Cuts at: 424 424
Size: 3425
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2564 2596 2564
Size: 32 3393
PstI C_TGCA'G
Cuts at: 275 472 1157 275
Size: 197 685 2543
Fragments arranged by size:
2543 685 197
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3425
PvuII CAG'CTG
Cuts at: 153 850 1210 153
Size: 697 360 2368
Fragments arranged by size:
2368 697 360
RsrII CG'GwC_CG
Cuts at: 1620 1620
Size: 3425
SacI G_AGCT'C
Cuts at: 439 439
Size: 3425
SalI G'TCGA_C
Cuts at: 462 462
Size: 3425
SapI GCTCTTCn'nnn_
Cuts at: 1448 1658 1448
Size: 210 3215
SbfI CC_TGCA'GG
Cuts at: 275 472 275
Size: 197 3228
ScaI AGT'ACT
Cuts at: 2942 2942
Size: 3425
SfcI C'TryA_G
Cuts at: 33 271 468 1153 2146 2337 33
Size: 238 197 685 993 191 1121
Fragments arranged by size:
1121 993 685 238 197 191
SfoI GGC'GCC
Cuts at: 857 1105 857
Size: 248 3177
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3425
SmaI CCC'GGG
Cuts at: 447 447
Size: 3425
SmlI C'TyrA_G
Cuts at: 580 1495 1987 2249 580
Size: 915 492 262 1756
Fragments arranged by size:
1756 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3425
SphI G_CATG'C
Cuts at: 478 978 478
Size: 500 2925
StyI C'CwwG_G
Cuts at: 107 559 913 107
Size: 452 354 2619
Fragments arranged by size:
2619 452 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 358 1008 1141 1600 1809 358
Size: 650 133 459 209 1974
Fragments arranged by size:
1974 650 459 209 133
TatI w'GTAC_w
Cuts at: 314 2940 314
Size: 2626 799
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2942 2974 2942
Size: 32 3393
Tth111I GACn'n_nGTC
Cuts at: 1222 1222
Size: 3425
XbaI T'CTAG_A
Cuts at: 456 456
Size: 3425
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 920 920
Size: 3425
XmaI C'CCGG_G
Cuts at: 445 445
Size: 3425
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI
BpmI BsaXI BsgI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI