Restriction map for
pF1KB6743
(Circular) (Six-base) MAPSORT of: pF1KB6743.seq Check: 5919 from: 1 to: 3410
pF1KB6743 3410 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 214 214
Size: 3410
AccI GT'mk_AC
Cuts at: 448 448
Size: 3410
Acc65I G'GTAC_C
Cuts at: 426 426
Size: 3410
AclI AA'CG_TT
Cuts at: 3250 3250
Size: 3410
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1235 1667 2414 2682 1235
Size: 432 747 268 1963
Fragments arranged by size:
1963 747 432 268
AflIII A'CryG_T
Cuts at: 762 1866 2843 762
Size: 1104 977 1329
Fragments arranged by size:
1329 1104 977
AgeI A'CCGG_T
Cuts at: 1798 1798
Size: 3410
AlwNI CAG_nnn'CTG
Cuts at: 715 2282 715
Size: 1567 1843
ApaLI G'TGCA_C
Cuts at: 2180 2180
Size: 3410
ApoI r'AATT_y
Cuts at: 414 2763 3019 414
Size: 2349 256 805
Fragments arranged by size:
2349 805 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3410
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3410
AvaI C'yCGr_G
Cuts at: 430 430
Size: 3410
BamHI G'GATC_C
Cuts at: 435 435
Size: 3410
BanI G'GyrC_C
Cuts at: 426 840 1088 1123 426
Size: 414 248 35 2713
Fragments arranged by size:
2713 414 248 35
BanII G_rGCy'C
Cuts at: 173 424 1454 173
Size: 251 1030 2129
Fragments arranged by size:
2129 1030 251
BbeI G_GCGC'C
Cuts at: 844 1092 844
Size: 248 3162
BbsI GAAGACnn'nnnn_
Cuts at: 406 406
Size: 3410
BciVI GTATCCnnnnn_n'
Cuts at: 1308 2069 3410 1308
Size: 761 1341 1308
Fragments arranged by size:
1341 1308 761
BclI T'GATC_A
Cuts at: 930 930
Size: 3410
BglII A'GATC_T
Cuts at: 233 326 925 2962 233
Size: 93 599 2037 681
Fragments arranged by size:
2037 681 599 93
BlpI GC'TnA_GC
Cuts at: 176 522 176
Size: 346 3064
Bme1580I G_kGCm'C
Cuts at: 1035 1128 2184 1035
Size: 93 1056 2261
Fragments arranged by size:
2261 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 193 622 703 1038 3182 193
Size: 429 81 335 2144 421
Fragments arranged by size:
2144 429 421 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1777 1777
Size: 3410
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 1465 1957 2255 586
Size: 879 492 298 1741
Fragments arranged by size:
1741 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3114 3114
Size: 3410
BsaAI yAC'GTr
Cuts at: 763 763
Size: 3410
BsaBI GATnn'nnATC
Cuts at: 6 924 2961 6
Size: 918 2037 455
Fragments arranged by size:
2037 918 455
BsaHI Gr'CG_yC
Cuts at: 841 1089 1791 3287 841
Size: 248 702 1496 964
Fragments arranged by size:
1496 964 702 248
BsaWI w'CCGG_w
Cuts at: 599 1120 1798 2072 2219 2630 599
Size: 521 678 274 147 411 1379
Fragments arranged by size:
1379 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 378 408 378
Size: 30 3380
BseYI C'CCAG_C
Cuts at: 835 1593 2170 2666 835
Size: 758 577 496 1579
Fragments arranged by size:
1579 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 262
Size: 3410
BsiEI CG_ry'CG
Cuts at: 104 952 998 2206 104
Size: 848 46 1208 1308
Fragments arranged by size:
1308 1208 848 46
BsiHKAI G_wGCw'C
Cuts at: 424 1202 1392 2184 424
Size: 778 190 792 1650
Fragments arranged by size:
1650 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 173 424 1035 1128 1202 1392 1454 2184 173
Size: 251 611 93 74 190 62 730 1399
Fragments arranged by size:
1399 730 611 251 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2630 2630
Size: 3410
BspHI T'CATG_A
Cuts at: 2586 2586
Size: 3410
BspMI ACCTGCnnnn'nnnn_
Cuts at: 214 460 674 976 1357 214
Size: 246 214 302 381 2267
Fragments arranged by size:
2267 381 302 246 214
BsrBI CCG'CTC
Cuts at: 50 1702 1756 3240 3405 50
Size: 1652 54 1484 165 55
Fragments arranged by size:
1652 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1322 1322
Size: 3410
BsrFI r'CCGG_y
Cuts at: 1408 1589 1798 1408
Size: 181 209 3020
Fragments arranged by size:
3020 209 181
BssSI C'ACGA_G
Cuts at: 1681 2039 1681
Size: 358 3052
BstAPI GCAn_nnn'nTGC
Cuts at: 193 787 193
Size: 594 2816
BstBI TT'CG_AA
Cuts at: 1771 1771
Size: 3410
BstYI r'GATC_y
Cuts at: 1 233 326 435 902 925 1260 1506 2518
Size: 232 93 109 467 23 335 246 1012
Cuts at: 2518 2604 2616 2962 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 246 232 109
93 86 23 12
BtgI C'CryG_G
Cuts at: 365 365
Size: 3410
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1360 1541 1704 1360
Size: 181 163 3066
Fragments arranged by size:
3066 181 163
BtsI GCAGTG_nn'
Cuts at: 653 653
Size: 3410
DraI TTT'AAA
Cuts at: 409 409
Size: 3410
DrdI GACnn_nn'nnGTC
Cuts at: 1116 1974 1116
Size: 858 2552
EaeI y'GGCC_r
Cuts at: 317 995 1169 1560 1587 2932 317
Size: 678 174 391 27 1345 795
Fragments arranged by size:
1345 795 678 391 174 27
EagI C'GGCC_G
Cuts at: 995 995
Size: 3410
EarI CTCTTCn'nnn_
Cuts at: 1433 1643 1433
Size: 210 3200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1618 1928 2074 3220 1618
Size: 310 146 1146 1808
Fragments arranged by size:
1808 1146 310 146
EcoICRI GAG'CTC
Cuts at: 422 422
Size: 3410
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1235 1667 2414 2682 1235
Size: 432 747 268 1963
Fragments arranged by size:
1963 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 549 549
Size: 3410
EcoRI G'AATT_C
Cuts at: 414 414
Size: 3410
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1429 1429
Size: 3410
FspI TGC'GCA
Cuts at: 1191 1191
Size: 3410
HaeII r_GCGC'y
Cuts at: 844 1092 2114 3093 844
Size: 248 1022 979 1161
Fragments arranged by size:
1161 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1252 1282 1284 1314 1252
Size: 30 2 30 3348
Fragments arranged by size:
3348 30 30 2
HincII GTy'rAC
Cuts at: 449 449
Size: 3410
KasI G'GCGC_C
Cuts at: 840 1088 840
Size: 248 3162
KpnI G_GTAC'C
Cuts at: 430 430
Size: 3410
MluI A'CGCG_T
Cuts at: 2843 2843
Size: 3410
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2081 2265 2081
Size: 184 3226
MscI TGG'CCA
Cuts at: 319 1171 319
Size: 852 2558
MslI CAynn'nnrTG
Cuts at: 1399 1526 1399
Size: 127 3283
MspA1I CmG'CkG
Cuts at: 521 835 1195 2208 2453 521
Size: 314 360 1013 245 1478
Fragments arranged by size:
1478 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1591 1591
Size: 3410
NarI GG'CG_CC
Cuts at: 841 1089 841
Size: 248 3162
NcoI C'CATG_G
Cuts at: 365 365
Size: 3410
NgoMIV G'CCGG_C
Cuts at: 1589 1589
Size: 3410
NruI TCG'CGA
Cuts at: 253 253
Size: 3410
NspI r_CATG'y
Cuts at: 463 963 1870 463
Size: 500 907 2003
Fragments arranged by size:
2003 907 500
PciI A'CATG_T
Cuts at: 1866 1866
Size: 3410
PmeI GTTT'AAAC
Cuts at: 409 409
Size: 3410
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2549 2581 2549
Size: 32 3378
PsiI TTA'TAA
Cuts at: 215 215
Size: 3410
PstI C_TGCA'G
Cuts at: 457 1142 457
Size: 685 2725
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3410
PvuII CAG'CTG
Cuts at: 835 1195 835
Size: 360 3050
RsrII CG'GwC_CG
Cuts at: 1605 1605
Size: 3410
SacI G_AGCT'C
Cuts at: 424 424
Size: 3410
SalI G'TCGA_C
Cuts at: 447 447
Size: 3410
SapI GCTCTTCn'nnn_
Cuts at: 1433 1643 1433
Size: 210 3200
SbfI CC_TGCA'GG
Cuts at: 457 457
Size: 3410
ScaI AGT'ACT
Cuts at: 2927 2927
Size: 3410
SfcI C'TryA_G
Cuts at: 33 258 453 1138 2131 2322 33
Size: 225 195 685 993 191 1121
Fragments arranged by size:
1121 993 685 225 195 191
SfoI GGC'GCC
Cuts at: 842 1090 842
Size: 248 3162
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3410
SmaI CCC'GGG
Cuts at: 432 432
Size: 3410
SmlI C'TyrA_G
Cuts at: 565 1480 1972 2234 565
Size: 915 492 262 1741
Fragments arranged by size:
1741 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3410
SphI G_CATG'C
Cuts at: 463 963 463
Size: 500 2910
StyI C'CwwG_G
Cuts at: 165 320 365 544 898 165
Size: 155 45 179 354 2677
Fragments arranged by size:
2677 354 179 155 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 993 1126 1585 1794 993
Size: 133 459 209 2609
Fragments arranged by size:
2609 459 209 133
TatI w'GTAC_w
Cuts at: 2925 2925
Size: 3410
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 144 176 2927 2959 144
Size: 32 2751 32 595
Fragments arranged by size:
2751 595 32 32
Tth111I GACn'n_nGTC
Cuts at: 1207 1207
Size: 3410
XbaI T'CTAG_A
Cuts at: 441 441
Size: 3410
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 372 905 372
Size: 533 2877
XmaI C'CCGG_G
Cuts at: 430 430
Size: 3410
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PciI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI