Restriction map for
pF1KB6731
(Circular) (Six-base) MAPSORT of: pF1KB6731.seq Check: 8431 from: 1 to: 3398
pF1KB6731 3398 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 338 436 338
Size: 98 3300
Acc65I G'GTAC_C
Cuts at: 414 414
Size: 3398
AclI AA'CG_TT
Cuts at: 3238 3238
Size: 3398
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1223 1655 2402 2670 1223
Size: 432 747 268 1951
Fragments arranged by size:
1951 747 432 268
AflIII A'CryG_T
Cuts at: 266 750 1854 2831 266
Size: 484 1104 977 833
Fragments arranged by size:
1104 977 833 484
AgeI A'CCGG_T
Cuts at: 1786 1786
Size: 3398
AlwNI CAG_nnn'CTG
Cuts at: 250 703 2270 250
Size: 453 1567 1378
Fragments arranged by size:
1567 1378 453
ApaLI G'TGCA_C
Cuts at: 2168 2168
Size: 3398
ApoI r'AATT_y
Cuts at: 402 2751 3007 402
Size: 2349 256 793
Fragments arranged by size:
2349 793 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3398
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3398
AvaI C'yCGr_G
Cuts at: 418 418
Size: 3398
BamHI G'GATC_C
Cuts at: 423 423
Size: 3398
BanI G'GyrC_C
Cuts at: 414 828 1076 1111 414
Size: 414 248 35 2701
Fragments arranged by size:
2701 414 248 35
BanII G_rGCy'C
Cuts at: 301 412 1442 301
Size: 111 1030 2257
Fragments arranged by size:
2257 1030 111
BbeI G_GCGC'C
Cuts at: 832 1080 832
Size: 248 3150
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3364
BciVI GTATCCnnnnn_n'
Cuts at: 1296 2057 3398 1296
Size: 761 1341 1296
Fragments arranged by size:
1341 1296 761
BclI T'GATC_A
Cuts at: 918 918
Size: 3398
BglII A'GATC_T
Cuts at: 913 2950 913
Size: 2037 1361
BlpI GC'TnA_GC
Cuts at: 510 510
Size: 3398
Bme1580I G_kGCm'C
Cuts at: 1023 1116 2172 1023
Size: 93 1056 2249
Fragments arranged by size:
2249 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 610 691 1026 3170 610
Size: 81 335 2144 838
Fragments arranged by size:
2144 838 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1765 1765
Size: 3398
Bpu10I CC'TnA_GC
Cuts at: 175 175
Size: 3398
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 1453 1945 2243 574
Size: 879 492 298 1729
Fragments arranged by size:
1729 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3102 3102
Size: 3398
BsaAI yAC'GTr
Cuts at: 315 751 315
Size: 436 2962
BsaBI GATnn'nnATC
Cuts at: 6 209 912 2949 6
Size: 203 703 2037 455
Fragments arranged by size:
2037 703 455 203
BsaHI Gr'CG_yC
Cuts at: 829 1077 1779 3275 829
Size: 248 702 1496 952
Fragments arranged by size:
1496 952 702 248
BsaWI w'CCGG_w
Cuts at: 587 1108 1786 2060 2207 2618 587
Size: 521 678 274 147 411 1367
Fragments arranged by size:
1367 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 823 1581 2158 2654 823
Size: 758 577 496 1567
Fragments arranged by size:
1567 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 337
Size: 3398
BsiEI CG_ry'CG
Cuts at: 104 940 986 2194 104
Size: 836 46 1208 1308
Fragments arranged by size:
1308 1208 836 46
BsiHKAI G_wGCw'C
Cuts at: 412 1190 1380 2172 412
Size: 778 190 792 1638
Fragments arranged by size:
1638 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 301 412 1023 1116 1190 1380 1442 2172 301
Size: 111 611 93 74 190 62 730 1527
Fragments arranged by size:
1527 730 611 190 111 93 74 62
BspEI T'CCGG_A
Cuts at: 2618 2618
Size: 3398
BspHI T'CATG_A
Cuts at: 2574 2574
Size: 3398
BspMI ACCTGCnnnn'nnnn_
Cuts at: 448 662 964 1345 448
Size: 214 302 381 2501
Fragments arranged by size:
2501 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1690 1744 3228 3393 50
Size: 1640 54 1484 165 55
Fragments arranged by size:
1640 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1310 1310
Size: 3398
BsrFI r'CCGG_y
Cuts at: 1396 1577 1786 1396
Size: 181 209 3008
Fragments arranged by size:
3008 209 181
BssSI C'ACGA_G
Cuts at: 1669 2027 1669
Size: 358 3040
BstAPI GCAn_nnn'nTGC
Cuts at: 775 775
Size: 3398
BstBI TT'CG_AA
Cuts at: 1759 1759
Size: 3398
BstYI r'GATC_y
Cuts at: 1 423 890 913 1248 1494 2506 2592 2604
Size: 422 467 23 335 246 1012 86 12
Cuts at: 2604 2950 1
Size: 346 449
Fragments arranged by size:
1012 467 449 422 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1348 1529 1692 1348
Size: 181 163 3054
Fragments arranged by size:
3054 181 163
BtsI GCAGTG_nn'
Cuts at: 641 641
Size: 3398
DraI TTT'AAA
Cuts at: 397 397
Size: 3398
DrdI GACnn_nn'nnGTC
Cuts at: 238 1104 1962 238
Size: 866 858 1674
Fragments arranged by size:
1674 866 858
EaeI y'GGCC_r
Cuts at: 983 1157 1548 1575 2920 983
Size: 174 391 27 1345 1461
Fragments arranged by size:
1461 1345 391 174 27
EagI C'GGCC_G
Cuts at: 983 983
Size: 3398
EarI CTCTTCn'nnn_
Cuts at: 118 1421 1631 118
Size: 1303 210 1885
Fragments arranged by size:
1885 1303 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1606 1916 2062 3208 1606
Size: 310 146 1146 1796
Fragments arranged by size:
1796 1146 310 146
EcoICRI GAG'CTC
Cuts at: 410 410
Size: 3398
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1223 1655 2402 2670 1223
Size: 432 747 268 1951
Fragments arranged by size:
1951 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 537 537
Size: 3398
EcoRI G'AATT_C
Cuts at: 402 402
Size: 3398
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 303 1417 303
Size: 1114 2284
FspI TGC'GCA
Cuts at: 1179 1179
Size: 3398
HaeII r_GCGC'y
Cuts at: 832 1080 2102 3081 832
Size: 248 1022 979 1149
Fragments arranged by size:
1149 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1240 1270 1272 1302 1240
Size: 30 2 30 3336
Fragments arranged by size:
3336 30 30 2
HincII GTy'rAC
Cuts at: 339 437 339
Size: 98 3300
KasI G'GCGC_C
Cuts at: 828 1076 828
Size: 248 3150
KpnI G_GTAC'C
Cuts at: 418 418
Size: 3398
MluI A'CGCG_T
Cuts at: 2831 2831
Size: 3398
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2069 2253 2069
Size: 184 3214
MscI TGG'CCA
Cuts at: 1159 1159
Size: 3398
MslI CAynn'nnrTG
Cuts at: 1387 1514 1387
Size: 127 3271
MspA1I CmG'CkG
Cuts at: 321 509 823 1183 2196 2441 321
Size: 188 314 360 1013 245 1278
Fragments arranged by size:
1278 1013 360 314 245 188
NaeI GCC'GGC
Cuts at: 1579 1579
Size: 3398
NarI GG'CG_CC
Cuts at: 829 1077 829
Size: 248 3150
NgoMIV G'CCGG_C
Cuts at: 1577 1577
Size: 3398
NspI r_CATG'y
Cuts at: 270 451 951 1858 270
Size: 181 500 907 1810
Fragments arranged by size:
1810 907 500 181
PciI A'CATG_T
Cuts at: 266 1854 266
Size: 1588 1810
PflMI CCAn_nnn'nTGG
Cuts at: 201 201
Size: 3398
PmeI GTTT'AAAC
Cuts at: 397 397
Size: 3398
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2537 2569 2537
Size: 32 3366
PstI C_TGCA'G
Cuts at: 383 445 1130 383
Size: 62 685 2651
Fragments arranged by size:
2651 685 62
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3398
PvuII CAG'CTG
Cuts at: 321 823 1183 321
Size: 502 360 2536
Fragments arranged by size:
2536 502 360
RsrII CG'GwC_CG
Cuts at: 1593 1593
Size: 3398
SacI G_AGCT'C
Cuts at: 412 412
Size: 3398
SalI G'TCGA_C
Cuts at: 337 435 337
Size: 98 3300
SapI GCTCTTCn'nnn_
Cuts at: 118 1421 1631 118
Size: 1303 210 1885
Fragments arranged by size:
1885 1303 210
SbfI CC_TGCA'GG
Cuts at: 445 445
Size: 3398
ScaI AGT'ACT
Cuts at: 2915 2915
Size: 3398
SfcI C'TryA_G
Cuts at: 33 352 379 441 1126 2119 2310 33
Size: 319 27 62 685 993 191 1121
Fragments arranged by size:
1121 993 685 319 191 62 27
SfoI GGC'GCC
Cuts at: 830 1078 830
Size: 248 3150
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3398
SmaI CCC'GGG
Cuts at: 420 420
Size: 3398
SmlI C'TyrA_G
Cuts at: 553 1468 1960 2222 553
Size: 915 492 262 1729
Fragments arranged by size:
1729 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3398
SphI G_CATG'C
Cuts at: 451 951 451
Size: 500 2898
StyI C'CwwG_G
Cuts at: 532 886 532
Size: 354 3044
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 981 1114 1573 1782 981
Size: 133 459 209 2597
Fragments arranged by size:
2597 459 209 133
TatI w'GTAC_w
Cuts at: 2913 2913
Size: 3398
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2915 2947 2915
Size: 32 3366
Tth111I GACn'n_nGTC
Cuts at: 1195 1195
Size: 3398
XbaI T'CTAG_A
Cuts at: 429 429
Size: 3398
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 893 893
Size: 3398
XmaI C'CCGG_G
Cuts at: 418 418
Size: 3398
XmnI GAAnn'nnTTC
Cuts at: 286 286
Size: 3398
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI BpmI BsaXI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI